The The Therapeutic Effects of Autologous Platelet-Rich Plasma Gel on Cutaneous Wound Healing in Rescued Horses The Effects of Autologous Platelet-Rich Plasma Gel on skin Wound Healing in Horses

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Δημοσιευμένα: Apr 12, 2023
Εκδόσεις:
2023-04-12 (1)
DOI: https://doi.org/10.12681/jhvms.29654
MT Sajjad
a:1:{s:5:"en_US";s:45:"University of Veterinary and Animal Sciences ";}
H Akbar
University of Veterinary and Animal Sciences
MA Khan
University of Veterinary and Animal Sciences
MH Mushtaq
University of Veterinary and Animal Sciences
SG Bokhari
University of Veterinary and Animal Sciences
MA Hayat
Northeast Agricultural University
G Mustafa
University of Veterinary and Animal Sciences
Περίληψη

This study explored the effect of platelet-rich plasma gel on skin wound healing in rescued horses. A total of twenty horses were used and allocated into two groups: PRP-treated Group A (n=10) and ASD-treated Group B (n=10). Group A animals were treated with autologous PRP gel, while group B animals were treated with sterile saline. A full-thickness (1.5 mm2) skin wounds were selected in each horse. Histopathological examination of wound tissue in horses was performed on days 8, 40, and 60. Wound epithelialization was assessed by HE staining, and collagen re-establishment was assessed by Masson’s trichrome staining. The CAT activity and MDA concentration were assessed in blood samples on days 8, 40, and 60. All data were subjected to statistical analysis. We observed a highly significant increase (P<0.01) in re-epithelization at days 40 to 60 in the PRP wounds compared to the ASD wounds. Collagen was well organized (P<0.01) in the PRP wounds compared with the ASD wounds at day 40 to day 60. MDA concentration was significantly decreased from day 40 to day 60 (P<0.05) in the PRP wounds than the ASD wounds. CAT activity showed no difference (P>0.05) between PRP wounds and ASD wounds. In conclusion, compared to ASD wounds, PRP wounds promoted cutaneous wound healing in horses by suppressing oxidative stress levels, accelerating wound epithelialization, and generating organized tissue, interlocking collagen bundles of dermal collagen. However, ultrasonographical assessment level study is needed to further investigate the effect of PRP gel on skin wound healing in rescued horses.


Keywords: Horses, Oxidative stress, Platelet-Rich Plasma, Sterile saline, Skin Wound Healing.

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Βιογραφικά Συγγραφέων
H Akbar, University of Veterinary and Animal Sciences

Assistant Prof. of Veterinary Surgery, Department of Veterinary Surgery and Pets Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

MA Khan, University of Veterinary and Animal Sciences

Prof. of Vet. surgery, Department of Veterinary Surgery and Pets Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

MH Mushtaq, University of Veterinary and Animal Sciences

Associate Prof. Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

SG Bokhari, University of Veterinary and Animal Sciences

Asoociate Prof. of Vet. Surgery, Department of Veterinary Surgery and Pets Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

MA Hayat, Northeast Agricultural University

Phd student of Veterinary Surgery, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University 150030, Harbin, China

G Mustafa, University of Veterinary and Animal Sciences

Lecturer, Department of Veterinary Pathology, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Αναφορές
Abood WN, Al-Henhena NA, Abood AN, Al-Obaidi MM, Ismail S, Abdulla
MA, Al Batran R (2015) Wound-healing potential of the fruit extract of
Phaleriamacrocarpa. Bosn J Basic Med Sci 15:25.
Abu-Seida AM (2015) Regenerative therapy for equine osteoarthritis: a concise review. Asian J Anim Vet Adv 10:500-508.
Aebi H (1984) Catalase in Vitro. Meth Enzymol 105:121-126.
Andia I, Maffulli N (2015) Muscle and tendon injuries: the role of biological
interventions to promote and assist healing and recovery. Arthroscopy
:999-1015.
Bigliardi PL, Alsagoff SA, El-Kafrawi HY, Pyon JK, Wa CT, Villa MA (2017)
Povidone iodine in wound healing: A review of current concepts and
practices. Int J Surg 44:260-8.
Broughton G, Rohrich RJ (2005) Wounds and scars. SRPS 10 (Part 1):1-54.
Camini FC, da Silva Caetano CC, Almeida LT, da Costa Guerra JF, de Mello
Silva B, de Queiroz Silva S, de Magalhães JC, de Brito Magalhães CL
(2017) Oxidative stress in Mayaro virus infection. Virus Res 236:1-8.
Carter AC, Jolly DG, Worden CE, Hendren DG, Kane CJM (2003) Platelet-richplasma gel promotes differentiation and regeneration during
equine woundhealing. ExpMolPathol 74:244-55.
Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B,
Moroni M, Carabelli A (2004) Platelet gel for healing cutaneous chronic
wounds. Tran ApherSci 30:145-51.
De la Fuente M (2014) “The immune system, a marker and modulator of the
rate of aging,” in Immunology of Aging, Massoud A and Rezaei N, Eds
Springer-Verlag Berlin Heidelberg, Germany:3-23
DeRossi R, Coelho AC, Mello GS, Frazílio FO, Leal CR, Facco GG, Brum
KB (2009) Effects of platelet-rich plasma gel on skin healing in surgical
wound in horses.ActaCirúrgBrasil 24:276-81.
Dole M, Wilson F, Fife W (1975) Hyperbaric hydrogen therapy: A possible
treatment for cancer. Sci 190:152-154.
Dugrillon A, Kluter H (2002) Current use of platelet concentrates for topical
application in tissue repair. TherTransfus Med 29:67-70.
Gawel S, Wardas M, Niedworok E, Wardas P (2004) MDA as a lipid peroxidation marker. WiadLek 57:453-455.
Griffiths HR, Møller L, Bartosz G, Bast A, Bertoni-Freddari C, Collins A,
Cooke M, Coolen S, Haenen G, Hoberg AM, Loft S (2002) Biomarkers.
MolAspects Med 23:101-208.
Halliwell B (1999) Antioxidant defence mechanism: from the beginning to
the end (of the beginning). Free Rad Res 31:261-272.
Halliwell B, Chirico S (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am J ClinNutr 57:715-725.
Hassan WU, Greiser U, Wang W (2014) Role of adipose-derived stem cells in
wound healing. Wound Rep Reg 22:313-25.
Huang S, Jiao J, Yan H (2016) Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a
rabbit model. ExpTher Med 11:177-182.
Iuchi Y, Roy D, Okada F, Kibe N, Tsunoda S, Suzuki S, Takahashi M, Yokoyama H, Yoshitake J, Kondo S, Fujii J (2010) Spontaneous skin damage and delayed wound healing in SOD1-deficient mice. Mol Cell Biochem 341:181-94.
Jee CH, Eom NY, Jang HM, Jung HW, Choi ES, Won JH, Hong IH, Kang
BT, Jeong DW, Jung DI (2016) Effect of autologous platelet-rich plasma
application on cutaneous wound healing in dogs. J Vet Sci 17:79-87.
Keller auf dem U, Ku¨min A, Braun S, Werner S (2006) Reactive oxygen
species and their detoxification in healing skin wounds. J InvestigDermatolSymp Proc 11:106-111.
Komarcevic A (2000) The modern approach to wound treatment. Med Pregl
:363-368.
Li J, Chen J, Kirsner R (2007) Pathophysiology of acute wound healing. Clinic Dermatol 25:9-18.
Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofacial Surg 62:489-96.
Mazzocca AD, McCarthy MB, Intravia J, Beitzel K, Apostolakos J, Cote MP,
Bradley J, Arciero RA (2013) An in vitro evaluation of the anti-inflammatory effects of platelet-rich plasma, ketorolac, and methylprednisolone. Arthroscopy: J ArthrRelSurg 29:675-83.
Miquel J (2009) An update of the oxidation-inflammation theory of aging: the
involvement of the immune system in oxi-inflamm-aging. Currpharmaceut Des 15:3003-26.
Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB (2014) Reactive oxygen
species in inflammation and tissue injury. Antioxi Red Sign 20:1126-67.
Ohkawa H, Ohishi W, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-358.
Palmieri B, Sblendorio V (2007) Oxidative stress tests: overview on reability
and use part I. Eur Rev Med PharmacolSci 11:309-342.
Rizzo M, Arfuso F, Giannetto C, Giudice E, Longo F, Di Pietro S, Piccione
G (2017) Cortisol levels and leukocyte population values in transported
and exercised horses after acupuncture needle stimulation. J Vet Behav
:56-61.
Robson MC (1997) The role of growth factors in the healing of chronic
wounds. Wound Rep Regenerat 5:12-7.
Sabol F, Dancakova L, Gal P, Vasilenko T, Novotny M, Smetana K, Lenhardt
L (2012) Immunohistological changes in skin wounds during the early
periods of healing in a rat model. Vet Med 57:77-82.
Sadek Y, Elballal S, Sharshar A, Elsunsafty M (2020) Platelets Rich Plasma
Accelerates Wound Healing: Histopathological Study. J Cur Vet Res 2:
-24.
Sen CK, Roy S (2008) Redox signals in wound healing. BiochimBiophysActa 1780:1348-1361.
Smith RG, Gassmann CJ, Campbell MS (2007) Platelet-rich plasma: properties and clinical applications. JLGH 2:73-78.
Soundia A, Hadaya D, Esfandi N, Gkouveris I, Christensen R, Dry SM,
Bezouglaia O, Pirih F, Nikitakis N, Aghaloo T, Tetradis S (2018) Zoledronate impairs socket healing after extraction of teeth with experimental
periodontitis. J Den Res 97:312-20.
Soundia A, Hadaya D, Esfandi N, Gkouveris I, Christensen R, Dry SM,
Bezouglaia O, Pirih F, Nikitakis N, Aghaloo T, Tetradis S (2018). Zoledronate impairs socket healing after extraction of teeth with experimental
periodontitis. J Dent Res 97:312-20.
Su L, Zheng J, Wang Y, Zhang W, Hu D (2019) Emerging progress on the
mechanism and technology in wound repair. Biomed Pharmacotherap
:109191.
Teng M, Huang Y, Zhang H (2014) Application of stems cells in wound healing-an update. Wound Rep Reg 22:151-60.
Toru I (2013) Molecular hydrogen: New antioxidant and anti-inflammatory
therapy for rheumatoid arthritis and related diseases. Curr Pharm Des
:6375-6381.
Van Meurs SJ, Gawlitta D, Heemstra KA, Poolman RW, Vogely HC, Kruyt
MC (2014) Selection of an optimal antiseptic solution for intraoperative
irrigation: an in vitro study. J Bone Joint Surg Am 96:285-291.
Velnar T, Bailey T, Smrkolj V (2009) The wound healing process: an overview
of the cellular and molecular mechanisms. J Int Med Res 37: 1528-1542.
Vendramin FS, Franco D, Schamall RF, Franco TR (2010) Utilização do plasma ricoemplaquetas (PRP) autólogoemenxertoscutâneosemcoelhos. Rev
Bras Cir Plast 25:4-10.
Vermeulen H, Westerbos SJ, Ubbink DT (2010) Benefit and harm of iodine in
wound care: a systematic review. J Hosp Infect 76:191-199.
Wang JH, Nirmala X (2016) Application of tendon stem/progenitor cells and
platelet-rich plasma to treat tendon injuries. Oper Tech Orthop 26:68-72.
Wang L, Qin W, Zhou Y, Chen B, Zhao X, Zhao H, Mi E, Wang Q, Ning J
(2017) Transforming growth factor β plays an important role in enhancing wound healing by topical application of Povidone-iodine. Sci Rep
:991.
Xue M, Jackson CJ (2015) Extracellular matrix reorganization during wound
healing and its impact on abnormal scarring. Adv Wound Care (New Rochelle) 4:119-136.
Yip WL (2015) Influence of oxygen on wound healing. Int Wound J 12:620-
Yolanda MM, Maria AV, Amaia FG, Marcos PB, Silvia PL, Dolores E, Jesús
OH (2014) Adult stem cell therapy in chronic wound healing. J Stem Cell
Res Ther 162:2
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