Effect of hCG administration on ovulation and estrus in Saanen goats subjected to short-term estrus synchronization protocol during the breeding season


Δημοσιευμένα: Jul 9, 2023
Ενημερώθηκε: 2023-07-09
Εκδόσεις:
2023-07-09 (2)
I Dogan
https://orcid.org/0000-0003-1976-1814
MB Toker
A Aktar
MM Yilmaz
D Udum
Περίληψη

This study aimed to compare the effect of hCG administered at 24 or 36 h following a short-term estrus synchronization treatment on the ovulation time and estrus parameters in non-lactating Saanen goats during the breeding season. The estrous cycles of does were synchronized with an intravaginal sponge containing 60 mg of medroxyprogesterone acetate (MAP) for six days, and an injection of 125 µg of d-cloprostenol at the time of sponge insertion in addition to an injection of 300 IU of eCG 24h before sponge removal. After removal of the sponges, does were injected intramuscularly either 1 ml physiological saline (0.9% NaCl) solution after 12 h (Group1/Control; n=10), 100 IU hCG after 24 h (Group2; n=9) or 100 IU hCG after 36 h (Group3; n=9).  Estrus behavior after sponge removal was observed twice daily for 84 h using teaser bucks and transrectal ovarian ultrasonography was performed twice a day for seven days to determine small, medium and large follicle numbers, luteal development and the time of ovulation. Blood samples were collected on the same days to determine serum progesterone (P4) and estradiol (E2) concentrations. No significant differences were observed in terms of estrus parameters, ovarian structure and serum P4 and E2 concentrations between the hCG-treated groups and the control group. Average values observed for all groups: estrous response (53.57%), the interval from sponge removal to estrus and ovulation (35.2 h and 67.86 h, respectively), duration of estrus (18.4 h), the interval from estrus onset to ovulation (50.37 h), ovulation rate (96.43%), number of ovulations (1.36), ovulatory follicle diameter (6.86 mm), corpus luteum diameter (8.22 mm), follicle and luteal growth rate (1.17 and 0.68 mm/day, respectively). In conclusion, administration of hCG at 24 or 36 h following a short-term estrous synchronization protocol does not affect ovulation time, estrus parameters, and serum P4 and E2 concentrations in goats during the breeding season.

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Αναφορές
Abecia, J.A., Forcada, F., González-Bulnes, A., 2012.Hormonal control of
reproduction in small ruminants. Animal Reproduction Science 130:
-179.
Alvarado-Espino, A.S., Menchaca, A., Meza-Herrera, C.A., Carrillo-Moreno, D.I., Zúñiga-García, S., Arellano-Rodríguez, F., Mellado,
M., Véliz, F.G., 2019b. Ovarian response is not affected by the stage
of seasonal anestrus or breed of goats when using a progesterone injection plus human chorionic gonadotropin-based protocol. Animal
Reproduction Science 204: 60-65.
Alvarado-Espino, A.S., Meza-Herrera, C.A., Carrillo, E., González-Álvarez, V.H., Guillen-Muñoz, J.M., Ángel-García, O., Mellado, M.,
Reproductive outcomes of Alpine goats primed with progesterone and treated with human chorionic gonadotropin during the
anestrus-to-estrus transition season. Animal Reproduction Science
: 133-138.
Alvarado-Espino, A.S., Menchaca, A., Meza-Herrera, C.A., Mellado, M.,
Arellano, F., Véliz, F., 2019a. Use of injectable progesterone and hCG
for fixed-time artificial insemination during the non-breeding season
in goats. Theriogenology 127: 21-25.
Chemineau, P., Martin, G.B., Saumande, J., Normant, E., 1988. Seasonalandhormonalcontrol of pulsatile LH secretion in thedairygoat (Caprahircus). Journal of ReproductionandFertility 83: 91-98.
Dogan, I., Konyali, A., Gunay, U., Yurdabak, S.,2008a. Comparison of
the effect of cronolone sponges and PMSG or cloprostenol on estrous
induction in Turkish Saanen goats. Polish Journal of Veterinary Sciences11: 29-34.
Dogan, I., Konyali, A., Tolu, C., Yurdabak, S., 2008b. Different estrous
induction protocols during the transition period in lactating Turkish
Saanen does following AI. ActaVeterinaria (Beograd)58: 259-266.
Dogan, I., Nur, Z., Dogan, S., 2016. Different progestagen treatment duration on estrous synchronization during the natural breeding season
in non-lactating Anatolian black goats. Animal Reproduction13: 806-
Dogan, I., Toker, M.B., Alcay, S., UdumKucuksen, D., 2020a. Comparison and assessment of ovarian follicular dynamics during the
breeding and non-breeding season in Saanen goats.Wiener TierärztlicheMonatsschrift107: 63-71.
Dogan, I., Toker, M.B., Alcay, S., UdumKucuksen, D., 2020b. Effect of
GnRH treatment following a short-term estrous induction protocol on
estrus and ovulation in Saanen goats, during the transitional period.
Journal of the Hellenic Veterinary Medical Society71: 2569-2576.
Fatet, A., Pellicer-Rubio, M.T., Leboeuf,B., 2011. Reproductive cycle of
goats. Animal Reproduction Science 124: 211-219.
Fonseca, J.F., Alvim, G.P., Souza-Fabjan, J.M.G., Oliveira, M.E.F., Brair,
V.L., Brandão, F.Z., Facó, O., 2017a. Reproductive features and of
an anti-inflammatory drug in estrus-induced dairy goats artificially
inseminated in a standing position with cervix immobilization. Reproductive Biology 17: 268-273.
Fonseca, J.F., Maffili, V.V., Rodrigues, M.T., Santos,A.D.F., Rovay, H.,
Pinto Neto, A., Brandão, F.Z., Torres, C.A.A., 2006. Effects of hCG
on progesterone concentrations an fertility in cyclic, lactating Alpine
goats. Animal Reproduction 3: 410-414.
Fonseca, J.F., Souza-Fabjan, J.M.G., Oliveira, M.E.F., Cruz, R.C., Esteves, L.V., Matos de Paiva, M.P.S.L., Brandão, F.Z., Mancio, A.B.,
b. Evaluation of cervical mucus and reproductive efficiency of
seasonally anovular dairy goats after short-term progestagen-based
estrous induction protocols with different gonadotropins. Reproductive Biology17: 363-369.
Fonseca, J.F., Torres, C.A.A., 2005. Administration of hCG 5 days after
breeding and reproductive performance in nulliparous dairy goats.
Reproduction in Domestic Animals 40: 495-499.
Ginther, O.J., Kot, K., 1994. Follicular dynamics during the ovulatory season in goats. Theriogenology 42: 987-1001.
González-Álvarez, V.H., Meza-Herrera, C.A., Leyva, C., Alvarado-Espino, A.S., Guillén-Muñoz, J.M., Rodríguez-Martínez, R., Véliz-Deras,
F.G., 2016. Effectiveness of different hCG and GnRH based protocols
in progesterone primed goats on estrus induction and reproductive
outcomes in out-off-season goats. Journal of Animal Research 6: 177-
Gonzalez-Bulnes A, Menchaca A, Martin GB, Martinez-Ros P, 2020. Seventy years of progestogen treatments for management of the sheep
estrous cycle: where we are and where we should go. Reproduction, Fertility and Development 32: 441-452.
Hameed, N., Khan, M.I.U.R., Ahmad,W., Abbas, M., Murtaza, A.,
Shahzad, M., Ahmad, N., 2020. Follicular dynamics, estrous response
and pregnancy rate following GnRH and progesterone priming with
or without eCG during non-breeding season in anestrous Beetal
goats. Small Ruminant Research 182: 73-77.
Hunter, M.G., Robinson, R.S., Mann, G.E., Webb, R., 2004. Endocrine
and paracrine control of follicular development and ovulation rate in
farm species. Animal Reproduction Science 82-83: 461-477.
Mani, A.U., McKelvey, W.A.C., Watson, E.D., 1996. Effect of undernutrition on gonadotropin profiles in non-pregnant, cycling goats. Animal
Reproduction Science 43: 25-33.
Morand-Fehr, P., Hervieu, J., Santucci, P., 1989. Notation de I’etatcorporel: a vosstylos. La Chèvre 175: 39-42.
Orita, J., Tanaka, T., Kamomae, H., Kaneda, Y., 2000. Ultrasonographic
observation of follicular and luteal dynamics during the estrous cycle
in Shiba goats. Journalof Reproduction and Development 46: 31-37.
Pietroski, A.C.C.A., Brandão, F.Z., de Souza, J.M.G., da Fonseca, J.F.,
Short, medium or long-term hormonal treatments for induction
of synchronized estrus and ovulation in Saanen goats during the nonbreeding season. RevistaBrasileira deZootecnia 42: 168-173.
Rodríguez-Martínez, R., Meza-Herrera, C.A., Tapia-Robles, K.I., Alvarado-Espino, A.S., Luna-Orozco, J.R., Leyva, C., Mellado, M.,
Véliz-Deras, F.G., 2018. Effect of two routes of administration of
human chorionic gonadotropin upon oestrus induction and reproductive outcomes in adult acyclic mix-breed goats. Journal of Applied
Animal Research 46: 190-194.
Rubianes, E., Menchaca, A., 2003. The pattern and manipulation of ovarian follicular growth in goats. Animal Reproduction Science 78: 271-
Saleh, M., Shahin, M., Wuttke, W., Gauly, M., Holtz, W., 2012. Pharmacokinetics of human chorionic gonadotropin R., 2007. Assessment of luteal function by ultrasonographic appearance and measurement of corpora lutea in goats. Animal Reproduction Science 97: 36-46.
Sun, S., Liu, S., Luo, J., Chen, Z., Li, C., Loor, J.J., Cao, Y., 2019. Repeated pregnant mare serum gonadotropin-mediated oestrous synchronization alters gene expression in the ovaries and reduces reproductive
performance in dairy goats. Reproduction in Domestic Animals 54:
-881.
Vilariño, M., Rubianes, E., Menchaca, A., 2011. Re-use of intravaginal
progesterone devices associated with the Short-term Protocol for
timed artificial insemination in goats. Theriogenology 75: 1195-1200.
Wildeus, S., 2000. Current concepts in synchronization of estrus: Sheep
and goats. Journal of Animal Science 77: 1-14.
Zarazaga, L.A., Gatica, M.C., Gallego-Calvo, I., Celi, L., Guzmán, J.L.,
The timing of oestrus, the preovulatory LH surge and ovulation
in BlanceAndaluza goats synchronized by intravaginal progestagen
sponge treatment is modified by season but not by body condition
score. Animal Reproduction Science 146: 170-175
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