Serologic and bioinformatics analysis of the Brucella cross reacting antigens

PDF (English)
Δημοσιευμένα: avr. 20, 2024
DOI: https://doi.org/10.12681/jhvms.33876
M Pourmahdi Borujeni
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
M Khosravi
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
D Gharibi
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
F Khademi Moghadam
Graduated Student of biology, Faculty of Basic Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
S Yousefinejad
Student of Bacteriology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Περίληψη

Brucella species are the cause of a zoonotic disease in humans and various species of animals. The accurate diagnosis of brucellosis could limit the prevalence of the disease. The current study aimed to identify the cross reacting antigens among Brucella and some of the bacteria that possessing antigens of this sort. A total of 36 positive and negative sheep serum samples in Rose Bengal test, enrolled in the current research. The antibody titers against Brucella abortus, Salmonella typhimurium, Pasteurella multocida, Yersinia enterocolitica, Proteus vulgaris and Escherichia coli were determined using the indirect and absorbed indirect ELISA. The cross reacting antigens were defined using the bioinformatics and western-blotting assay. The positive samples have significant IgG antibody titer against all of the tested bacterial strains. There was a significant difference (P≤0.05) between the results of the direct and absorbed ELISA for delineating the anti- Brucella antibodies. Immunoblot assays revealed reactivity of the antibodies with the several protein bands in the other bacteria. Also, there was a significant correlation between % sequence and the structural identity (P≤0.001). Two proteins including Cu/Zn superoxide dismutase and methionine aminopeptidase of the mentioned bacteria showed a significantly higher similarity to Brucella proteins (P ≤0.001). These observations suggested the existence of variable IgM and IgG antibodies titer against other bacteria in Brucella positive samples; additionally, the anti-Brucella antibody in each of the positive samples had different cross reactivity against the common antigens of the other bacteria.

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Creative Commons License

Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.

Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.

Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.

Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc

Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.

Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Alaidan AA, Saba I, Alaiya AA, Al-Hokail AA, Al-Azragi TA, Elfaki MG (2019) Diagnosis of Human Brucellosis by Immunoblotting of Serum IgG Antibodies. EC Microbiol. 15, 198-205.
Al-Dahouk S, Nockler K, Scholz HC, Tomaso H, Bogumi lR, Neubauer H (2006) Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections. J. Immunol. Methods. 309, 34-47.
Bounaadja L, Albert D, Chénais B, Hénault S, Zygmunt MS, Poliak S, Garin-Bastuji B (2009) Real-time PCR for identification of Brucella spp.: a comparative study of IS711, bcsp31 and per target genes. Vet Microbiol. 137, 156-164. doi: 10.1016/j.vetmic.2008.12.023.
Buzgan T, Karahocagil MK, Irmak H, Baran AI, Karsen H, Evirgen O, Akdeniz H (2010) Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int. J. Infect. Dis., 14, e469-78. doi: 10.1016/j.ijid.2009.06.031.
Cassataro J, Delpino MV, Velikovsky CA, Bruno L, Fossati CA, Baldi PC (2002) Diagnostic usefulness of antibodies against ribosome recycling factor from Brucella melitensis in human or canine brucellosis. Clin. Diagn. Lab Immunol. 9, 366-369. doi: 10.1128/cdli.9.2.366-369.2002.
Chaudhuri P, Prasad R, Kumar V, Gangaplara A (2010) Recombinant OMP28 antigen-based indirect ELISA for serodiagnosis of bovine brucellosis. Mol. Cell. Probes.,24, 142-145. doi: 10.1016/j.mcp.2009.12.002.
Chirhart-Gilleland RL, Kovach ME, Elzer PH, Jennings SR, Roop RM (1998) Identification and characterization of a 14-kilodalton Brucella abortus protein reactive with antibodies from naturally and experimentally infected hosts and T lymphocytes from experimentally infected BALB/c mice. Infect Immun. 66, 4000-4003. doi: 10.1128/IAI.66.8.4000-4003.1998.
Corbel MJ (1985) Recent advances of Brucella antigens and their serological cross-reactions. Vet Bull., 55, 927-942.
Debbarh HS, Cloeckaert A, Zygmunt MS, Dubray G (1995) Identification of sero-reactive Brucella melitensis cytosoluble proteins which discriminate between antibodies elicited by infection and Rev.1 vaccination in sheep. Vet Microbiol. 44, 37-48. doi: 10.1016/0378-1135(94)00058-5.
Delpino MV, Cassataro J, Fossati CA, Baldi PC (2003) Antibodies to the CP24 protein of Brucella melitensis lack diagnostic usefulness in ovine brucellosis. Vet Microbiol. 93, 101-107.doi: 10.1016/s0378-1135(03)00015-4.
Edmonds MD, WardFM, O’HaraTM, Elzer PH (1999) Use of western immunoblot analysis for testing moose serum for Brucella suis biovar 4 specific antibodies. J. Wildl. Dis. 35, 591-595. doi: 10.7589/0090-3558-35.3.591.
Falcao MVD, Santana VLA, Correa FN, Tenorio JAB, Mota RA (2019) Development and standardization of a western blotting test for detection of antibodies against B. abortus. Arq Bras Med Vet Zootec.71, 160-6.
Godfroid J, Nielsen K, Saegerman C (2010) Diagnosis of brucellosis in livestock and wildlife. Croat. Med. J., 51, 296-305. doi:10.3325/cmj.2010.51.296.
Goldbaum FA, Velikovsky CA, Baldi PC, Mörtl S, Bacher A, Fossati CA (1999) The 18-kDa cytoplasmic protein of Brucella species -an antigen useful for diagnosis--is a lumazine synthase. J. Med Microbiol.48, 833-839. doi: 10.1099/00222615-48-9-833.
Gomez G, Pei J, Mwangi W, Adams LG, Rice-Ficht A, Ficht TA (2013) Immunogenic and invasive properties of Brucella melitensis 16M outer membrane protein vaccine candidates identified via a reverse vaccinology approach. PLoS One. 8, e59751. doi: 10.1371/journal.pone.0059751.
Hans R, Yadav PK, Sharma PK, Boopathi M, Thavaselvam D(2020) Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis. Sci Rep. 10, 8543. doi: 10.1038/s41598-020-65347-9.
Kim JY, Sung SR, Lee K, Lee HK, Kang SI, Lee JJ, Jung SC, Park YH, Her M (2014). Immunoproteomics of Brucella abortus RB51 as candidate antigens in serological diagnosis of brucellosis. Vet Immunol Immunopathol, 160, 218-224. doi: 10.1016/j.vetimm.2014.05.009. Epub 2014 May 21.
Ko KY, Kim JW, Her M, Kang SI, Jung SC, Cho DH, Kim JY (2012) Immunogenic proteins of Brucella abortus to minimize cross reactions in brucellosis diagnosis. Vet Microbiol. 156, 374-380. doi: 10.1016/j.vetmic.2011.11.011. Epub 2011 Nov 22.
Lindler LE, Hadfield TL, Tall BD, Snellings NJ, Rubin FA, Van De Verg LL, Hoover D, Warren RL (1996) Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infect Immun. 64, 2490-2499. doi: 10.1128/iai.64.7.2490-2499.1996.
Lotfi Z, Pourmahdi Borujeni M, Ghorbanpoor M, GhadrdanMashhadi AR (2020) Seroprevalence and risk factors of brucellosis in Arabian horses. Vet. Med. Sci.2, 1-7. doi.org/10.1002/vms3.759
Mahajan NK, Kulshreshtha RC, Malik G, Dahiya JP (2005) Immunogenicity of major cell surface protein(s) of Brucella melitensis Rev 1. Vet. Res. Commun.29, 189-199. doi: 10.1023/b:verc.0000047500.20855.7a.
Manat Y, Shustov AV, Evtehova E, Eskendirova SZ (2016) Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species. Open Vet J. 6, 71-77. doi: 10.4314/ovj.v6i2.1. Epub 2016 May 20.
Mostafaie A, ABD Alalizadeh J, Noumanpour B, Karimi R,Bahrami Y (2005) Immunogens of Brucella abortus S19 identified by two-dimensional gel electrophoresis and immunoblotting. Iran. J. Med. Sci, 30, 10-15.
Nielsen K, Heck F, Wagner G, Stiller J, Rosenbaum B, Pugh R, Flores E (1984) Comparative assessment of antibody isotypes to Brucella abortus by primary and secondary binding assays. Prev Vet Med. 2, 197-204.
Nielsen K, Smith P, Widdison J, Gall D, Kelly L, Kelly W, Nicoletti P (2004) Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7.
Vet Microbiol. 100, 25-30. doi: 10.1016/j.vetmic.2003.12.010.Nielsen K, YuWL (2010) Serological diagnosis of brucellosis. Prilozi.31, 65-89.PMID: 20703184.
Pajuaba AC, Silva DA, Almeida KC, Cunha‐Junior JP, Pirovani CP, Camillo LR, Mineo JR (2012) Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle. Proteomics. 12, 820-831.doi: 10.1002/pmic.201100185.
Patra KP, Saito M, Atluri VL, Rolan HG, Young B, Kerrinnes T, Smits H, Ricaldi JN, Gotuzzo E, Gilman RH, Tsolis RM (2014) A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLOS Negl. Trop. Dis. 8, e2926. doi: 10.1371/journal.pntd.0002926.
Poester FP, Nielsen K, Samartino LE, Yu WL (2010) Diagnosis of Brucellosis. Open Vet. J. 4, 46-60. doi: 10.2174/1874318801004010046.
Robles CA, NielsenK, Gall D, Willems P (2009) Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers. Vet. Immunol. Immunopathol.127, 153-155. doi: 10.1016/j.vetimm.2008.09.007.
Rossetti OL, Arese AI, Boschiroli ML, Cravero SL (1996) Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis. J. Clin. Microbiol. 34, 165-169. doi: 10.1128/jcm.34.1.165-169.1996.
Salih-AljDebbarh H, Cloeckaert A, Bezard G, Dubray G, Zygmunt MS (1996) Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep. ClinDiagn Lab Immunol. 3, 305-308. doi: 10.1128 cdli.3.3.305-308.1996.
Schumaker BA, Mazet JA, Gonzales BJ, Elzer PH, Hietala SK, Ziccardi MH (2010): Evaluation of the Western immunoblot as a detection method for Brucella abortus exposure in elk. J. Wildl. Dis. 46, 87-94. doi: 10.7589/0090-3558-46.1.87.
Shemesh AA,Yagupsky P (2011) Limitations of the standard agglutination test for detecting patients with Brucella melitensis bacteremia. Vector-Borne Zoonotic Dis. 11, 1599-1601. doi: 10.1089/vbz.2011.0704.
World Organisation for Animal Health (OIE), (2021a) Chapter 1.1.6; Principles and methods of validation of diagnostic assays for infectious diseases (version adopted in May 2013). In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2021, 72-87.https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/1.01.06_VALIDATION.pdf
World Organisation for Animal Health (OIE), (2021b) Chapter 2.4.3; Brucellosis (Brucellaabortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis) (version adopted in May 2016). In:manual ofdiagnostictests and vaccines for terrestrial animals 2021, 355-398. https://www.oie.int/fileadmin/Home/eng/ ealth_standards/tahm/3.01.04_BRUCELLOSIS.pdf
Zygmunt MS, Cloeckaert A, Dubray G (1994) Brucella melitensis cell envelope protein and lipopolysaccharide epitopes involved in humoral immune responses of naturally and experimentally infected sheep. J. Clin. Microbiol. 32, 2514-2522. doi: 10.1128/jcm.32.10.2514-2522.1994
Τα περισσότερο διαβασμένα άρθρα του ίδιου συγγραφέα(s)