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Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation
Published:
Jul 10, 2024
Versions:
2024-07-10 (1)
Keywords:
Goat ß-glucan mitochondrial membrane potential DNA fragmentation antioxidant activity
Abstract
This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen.
In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentrations of 1 mM (βG1), 2 mM (βG2), and 4 mM (βG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation, and determination of total antioxidant activity.
The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the βG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P<0.05). While the DNA fragmentation rate of the control group was higher than βG4 group (P<0.05), it was similar to βG1 and βG2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates.
In line with our results, supplementation of 1mM, 2 mM and 4 mM β-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.
Article Details
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Ustuner, B., Yılmaz, M., Huraydin, O., Cimen, T., Ak Sonat, F., Aktar, A., Gokce, E., Karakci, D., Sengul, E., Soylu, M., & Nur, Z. (2024). Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation. Journal of the Hellenic Veterinary Medical Society, 75(2). https://doi.org/10.12681/jhvms.34504
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- Vol. 75 No. 2 (2024)
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- Research Articles
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