Detection of highly potent Stx2a-encoding gene in pig fecal DNA in Serbia: Could commercial pig farms in Serbia act as source of human STEC infections?
Περίληψη
Shiga toxin-producing Escherichia coli (STEC) strains are major zoonotic food-borne pathogens of public health importance. Although cattle are recognized as a major reservoir of STEC, these strains have been frequently isolated from the intestinal content of swine, therefore swine shed STEC in the feces. Shiga toxin genes were subtyped to assess public health implications of STEC. By subtyping the stx2 gene subtypes in swine we established the presence of the stx2e gene in 9 out of 82 stx2 positive samples. Furthermore, the zoonotically significant stx2a gene in 3 samples, which, although with a low prevalence, is of great importance for public health, enabling completion of the epidemiological picture of STEC in Serbia.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Vračar, V., Mitrović, J., Lalošević, V., Kozoderović, G., & Petrovic, D. (2025). Detection of highly potent Stx2a-encoding gene in pig fecal DNA in Serbia: Could commercial pig farms in Serbia act as source of human STEC infections?. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 76(1), 8665–8670. https://doi.org/10.12681/jhvms.36705
- Τεύχος
- Τόμ. 76 Αρ. 1 (2025)
- Ενότητα
- Research Articles
Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.
Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.
Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.
Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc
Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Altwegg M (1995) General problems associated with diagnostic applications
of amplification methods. J Microbiol Methods 23: 21-30.
Arancia S, Iurescia M, Lorenzetti S, Stravino F, Buccella C, Caprioli A,
Franco A, Battisti A, Morabito S, Tozzoli R (2019) Detection and isolation
of Shiga Toxin-producing Escherichia coli (STEC) strains in
caecal samples from pigs at slaughter in Italy. Vet Med Sci 5:462-469.
Ateba CN, Mbewe M (2011) Detection of Escherichia coli O157:H7 virulence
genes in isolates from beef, pork, water, human and animal
species in the northwest province, South Africa: public health implications.
Res. Microbiol 162:240-248.
Bai X, Fu S, Zhang J, Fan R, Xu Y, Sun H, He X, Xu J, Xiong Y (2018)
Identification and pathogenomic analysis of an Escherichia coli strain
producing a novel Shiga toxin 2 subtype. Sci Rep 8: 6756.
Bai X, Scheutz F, Dahlgren HM, Hedenström I, Jernberg C (2021) Characterization
of clinical Escherichia coli strains producing a novel Shiga
toxin 2 subtype in Sweden and Denmark. Microorganisms 9(11):
Baldo V, Salogni C, Giovannini S, D’Incau M, Boniotti MB, Birbes L, Pitozzi
A, Formenti N, Grassi A, Pasquali P, Alborali GL (2020) Pathogenicity
of Shiga toxin type 2e Escherichia coli in pig colibacillosis.
Front Vet Sci (7): 545818.
Baranzoni GM, Fratamico PM, Gangiredla J, Patel I, Bagi LK, Delannoy
S, Fach P, Boccia F, Anastasio A, Pepe T (2016) Characterization of
Shiga toxin subtypes and virulence genes in porcine Shiga toxin-producing
Escherichia coli. Front Microbiol 7:574
Beutin L, Martin A (2012) Outbreak of Shiga toxin-producing Escherichia
coli (STEC) O104: H4 infection in Germany causes a paradigm
shift with regard to human pathogenicity of STEC strains. J Food
Prot 75(2): 408-418.
Bielaszewska M, Mellmann A, Bletz S, Zhang W, Köck R, Kossow A,
Prager R, Fruth A, Orth-Höller D, Marejková M, Morabito S (2013)
Enterohemorrhagic Escherichia coli O26: H11/H−: a new virulent
clone emerges in Europe. Clin Infect Dis 56(10): 1373-1381.
Bosilevac JM, Koohmaraie M (2011) Prevalence and characterization of
non-O157 Shigatoxin-producing Escherichia coli isolates from commercial
ground beef in the United States. Appl Environ Microbiol
:2103-2112.
Capps KM, Ludwig JB, Shridhar PB, Shi X, Roberts E, DebRoy C, Cernicchiaro
N, Phebus RK, Bai J, Nagaraja TG (2021) Identification,
Shiga toxin subtypes and prevalence of minor serogroups of Shiga
toxin-producing Escherichia coli in feedlot cattle feces. Sci Rep 11:
Cha W, Fratamico PM, Ruth LE, Bowman AS, Nolting JM, Manning SD,
Funk JA (2018) Prevalence and characteristics of Shiga toxin-producing
Escherichia coli in finishing pigs: implications on public health.
Int J Food Microbiol 264: 8-15.
Cobeljic M, Dimic B, Opacic D, Lepsanovic Z, Stojanovic V, Lazic S
(2005) The prevalence of Shiga toxin-producing Escherichia coli in
domestic animals and food in Serbia. Epidemiol Infect 133: 359-366.
Cobeljic M, Lepsanovic Z, Velimirovic S (1995) Infrequent finding of
verotoxin-producing Escherichia coli in diarrheal stools in Belgrade,
Serbia. Scand J Infect Dis 27: 427-428
Colello R, Cáceres ME, Ruiz MJ, Sanz M, Etcheverría AI, Padola NL
(2016) From farm to table: follow-up of Shiga toxin-producing Escherichia
coli throughout the pork production chain in Argentina. Front
Microbiol 7: 93.
Ercoli L, Farneti S, Zicavo A, Mencaroni G, Blasi G, Striano G, Scuota
S (2016) Prevalence and characteristics of verotoxigenic Escherichia
coli strains isolated from pigs and pork products in Umbria and
Marche regions of Italy. Int J Food Microbiol 232: 7-14.
Fadel HM, Afifi R, Al-Qabili DM (2017) Characterization and zoonotic
impact of Shiga toxin producing Escherichia coli in some wild bird
species. Vet World. 10:1118-1128.
Feng PC, Jinneman K, Scheutz F and Monday SR 2011. Specificity of
PCR and serological assays in the detection of Escherichia coli Shiga
toxin subtypes. Appl Environ Microbiol 77(18): 6699-6702.
Fitzgerald SF, Beckett AE, Palarea-Albaladejo J, McAteer S, Shaaban S,
Morgan J, Ahmad NI, Young R, Mabbott NA, Morrison L, Bono JL,
Gally DL, McNeilly TN (2019) Shiga toxin sub-type 2a increases the
efficiency of Escherichia coli O157 transmission between animals
and restricts epithelial regeneration in bovine enteroids. PLOS Pathogens
(10): e1008003.
Gill A, Dussault F, McMahon T, Petronella N, Wang X, Cebelinski E,
Scheutz F, Weedmark K, Blais B, Carrillo C (2022) Characterization
of atypical Shiga toxin gene sequences and description of Stx2j, a
new subtype. J Clin Microbiol 60(3): 02229-21.
Goma MKE, Indraswari A, Haryanto A, Widiasih DA (2019) Detection of
Escherichia coli O157:H7 and Shiga toxin 2a gene in pork, pig feces,
and clean water at Jagalan slaughterhouse in Surakarta, Central Java
Province, Indonesia. Vet World 12:1584-1590.
Haque M, Bosilevac JM, Chaves BD (2022) A review of Shiga-toxin producing
Escherichia coli (STEC) contamination in the raw pork production
chain. Int J Food Microbiol 109832.
Karve SS, Weiss AA (2014) Glycolipid binding preferences of Shiga toxin
variants. PLOS One 9(7): 101173.
Lindsey RL, Prasad A, Feldgarden M, Gonzalez-Escalona N, Kapsak C,
Klimke W, Melton-Celsa A, Smith P, Souvorov A, Truong J, Scheutz
F (2023) Identification and Characterization of ten Escherichia coli
Strains Encoding Novel Shiga Toxin 2 Subtypes, Stx2n as Well as
Stx2j, Stx2m, and Stx2o, in the United States. Microorganisms
(10): 2561.
Lodato PB (2021) The effect of two ribonucleases on the production of
Shiga toxin and stx-bearing bacteriophages in Enterohaemorrhagic
Escherichia coli. Sci Rep 11: 18372.
Meng Q, Bai X, Zhao A, Lan R, Du H, Wang T, Shi C, Yuan X, Bai X, Ji S,
Jin D (2014) Characterization of Shiga toxin-producing Escherichia
coli isolated from healthy pigs in China. BMC Microbiol 14(1): 1-14.
Nastasijevic I, Schmidt JW, Boskovic M, Glisic M, Kalchayanand N,
Shackelford SD, Wheeler TL, Koohmaraie M, Bosilevac JM (2020)
Seasonal prevalence of Shiga toxin-producing Escherichia coli on
pork carcasses for three steps of the harvest process at two commercial
processing plants in the United States. Appl Environ Microbiol
(1): 01711-20.
Rahn K, Wilson JB, McFadden KA, Read SC, Ellis AG, Renwick SA,
Clarke RC, Johnson RP (1996) Comparison of Vero cell assay and
PCR as indicators of the presence of verocytotoxigenic Escherichia
coli in bovine and human fecal samples. Appl Environ Microbiol
(12): 4314-4317.
Remfry SE, Amachawadi RG, Shi X, Bai J, Tokach MD, Dritz SS, Goodband
RD, Derouchey JM, Woodworth JC, Nagaraja TG (2021) Shiga
Toxin-Producing Escherichia coli in Feces of Finisher Pigs: Isolation,
Identification, and Public Health Implications of Major and Minor
Serogroupsdagger. J Food Prot 84: 169-180.
Scheutz F, Teel LD, Beutin L, Pierard D, Buvens G, Karch H, Mellmann
A, Caprioli A, Tozzoli R, Morabito S, Strockbine NA (2012) Multicenter
evaluation of a sequence-based protocol for subtyping Shiga
toxins and standardizing Stx nomenclature. J Clin Microbiol 50:
-2963.
Skinner C, Patfield S, Khalil R, Kong Q, He X (2016) New Monoclonal
Antibodies against a Novel Subtype of Shiga Toxin 1 Produced by
Enterobacter cloacae and Their Use in Analysis of Human Serum.
mSphere 1(1): 10-1128.
Smith JL, Fratamico PM, Gunther IV NW (2014) Shiga toxin-producing
Escherichia coli. Adv Appl Microbiol 86: 145-197.
Soborg B, Lassen SG, Muller L, Jensen T, Ethelberg S, Mølbak K,
Scheutz F (2013) A verocytotoxin-producing E. coli outbreak with
a surprisingly high risk of haemolytic uraemic syndrome, Denmark,
September-October 2012. Euro Surveill 18(2): 20350.
Tseng M, Fratamico PM, Bagi L, Manzinger D, Funk JA (2015) Shiga
toxin-producing E. coli (STEC) in swine: prevalence over the finishing
period and characteristics of the STEC isolates. Epidemiol Infect
(3): 505-514.
Tseng M, Fratamico PM, Manning SD, Funk JA (2014) Shiga toxin-producing
Escherichia coli in swine: the public health perspective. Anim
Health Res Rev 15:63-75.
Yang X, Bai X, Zhang J, Sun H, Fu S, Fan R, He X, Scheutz F, Matussek
A, Xiong Y. (2020) Escherichia coli strains producing a novel Shiga
toxin 2 subtype circulate in China. Int J Med Microbiol 310: 151377.
