Detection of Staphylococcal Enterotoxin, Methicillin-Resistant, Panton- Valentine Leukocidin Genes and Antibiotic Susceptibility in Staphylococci and Current Bacteria Associated with Subclinical Mastitis in Cattle
Περίληψη
Bovine mastitis is a significant infectious disease affecting dairy animals, resulting in substantial economic losses. Staphylococci are the predominant causative agents of mastitis. This study aimed to identify the bacteria isolated from cow's milk with subclinical mastitis using MALDI-TOF mass spectrometry, assess their antibiotic susceptibility, and examine the presence of staphylococcal enterotoxin, pvl (Panton-Valentine Leukocidin), and mecA genes in Staphylococcus spp. Out of 301 milk samples, 73 isolates were identified as bacteria, with 40 (54.8%) being Staphylococci. The prevalence of antibiotic resistance among Staphylococcus spp. was as follows: benzylpenicillin 57.5% (n:23), tigecycline 37.5% (n:15), tetracycline 22.5% (n:9), erythromycin 17.5% (n:7), gentamicin 12.5% (n:5), cefoxitin 10.0% (n:4), trimethoprim/sulfamethoxazole 5.0% (n:2), ciprofloxacin 2.5% (n:1), and rifampicin 2.5% (n:1). Among the Staphylococcus haemolyticus isolates, 5 (11.62%) carried the mecA gene, and interestingly, 3 of these were phenotypically sensitive to cefoxitin despite harboring the mecA gene. However, none of the isolates carried the enterotoxin and pvl genes. This study was emphasized that antibiotic resistance of staphylococci isolated from subclinical with mastitis cows raised but these isolates were absent staphylococcal enterotoxin and pvl genes.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Ünal, N., Şavluk, M., & Kıymacı, M. (2025). Detection of Staphylococcal Enterotoxin, Methicillin-Resistant, Panton- Valentine Leukocidin Genes and Antibiotic Susceptibility in Staphylococci and Current Bacteria Associated with Subclinical Mastitis in Cattle. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 75(4), 8363–8370. https://doi.org/10.12681/jhvms.37159
- Τεύχος
- Τόμ. 75 Αρ. 4 (2024)
- Ενότητα
- Research Articles
Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.
Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.
Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.
Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc
Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Abed AH, Menshawy AMS, Zeinhom MMA, et al (2021) Subclinical
Mastitis in Selected Bovine Dairy Herds in North Upper Egypt: Assessment
of Prevalence, Causative Bacterial Pathogens, Antimicrobial
Resistance and Virulence-Associated Genes. Microorganisms 9(6).
Ali T, Kamran RA, Wazir I, et al (2021) Prevalence of Mastitis Pathogens
and Antimicrobial Susceptibility of Isolates From Cattle and Buffaloes
in Northwest of Pakistan. Front Vet Sci 8:746755. https://doi.
org/10.3389/fvets.2021.746755
Bal EB, Bayar S, Bal MA, (2010) Antimicrobial susceptibilities of coagulase-
negative staphylococci (CNS) and streptococci from bovine
subclinical mastitis cases. J Microbiol 48(3): 267-274. https://doi.
org/10.1007/s12275-010-9373-9
Bentayeb L, Akkou M, Zennia SS, et al (2023) Impacts of subclinical
mastitis on milk quality, clotting ability and microbial resistance of
the causative Staphylococci, LAR, 29: 105-111. https://www.largeanimalreview.
com/index.php/lar/article/view/633
Chambers HF, (1997) Methicillin resistance in staphylococci: molecular
and biochemical basis and clinical implications. Clin Microbiol Rev
(4): 781-791. https://doi.org/10.1128/CMR.10.4.781
Constable PD, Hinchcliff KW, Done SH, et al (2017): Veterinary Medicine-
e-Book: A Textbook of the Diseases of Cattle, Horses, Sheep,
Pigs and Goats 11th ed.,Elsevier, Amsterdam, The Netherlands, pp.
-465.
Demil E, Teshome L, Kerie Y, et al (2022) Prevalence of subclinical mastitis,
associated risk factors and antimicrobial susceptibility of the
pathogens isolated from milk samples of dairy cows in Northwest
Ethiopia. Prev Vet Med 205, 105680. https://doi.org/10.1016/j.prevetmed.
105680
Deng J, Liu K, Wang K, et al (2023) The prevalence of coagulase-negative
staphylococcus associated with bovine mastitis in China and its
antimicrobial resistance rate: a meta-analysis. J Dairy Res 90(2): 158-
Erskine RJ (2020) Mastitis in cattle. In: MSD Manual. Veterinary Manual.
Available from: https://www.msdvetmanual.com/reproductive-system/
mastitis-in-large-animals/mastitis-in-cattle?query=mastitis%20
in%20cattle [accessed 14 August 2023].
Harkins CP, Pichon B, Doumith M, et al (2017) Methicillin-resistant
Staphylococcus aureus emerged long before the introduction of
methicillin into clinical practice. Genome Biol 18(1): 130. https://doi.
org/10.1186/s13059-017-1252-9
Hoque MN, Das ZC, Rahman A, et al (2018) Molecular characterization
of Staphylococcus aureus strains in bovine mastitis milk in
Bangladesh. Int J Vet Sci Med 6(1):53-60. https://doi.org/10.1016/j.
ijvsm.2018.03.008
Ibrahim N, Regassa F, Yilmaz T, et al (2023) Impact of subclinical mastitis
on uterine health, reproductive performances and hormonal profile
of Zebu x Friesian crossbred dairy cows in and around Jimma town
dairy farms, Ethiopia. Heliyon 9(6):e16793. https://doi.org/10.1016/j.
heliyon.2023.e16793
Kulangara V, Nair N, Sivasailam A, et al (2017) Genotypic and phenotypic
beta-lactam resistance and presence of PVL gene in Staphylococci
from dry bovine udder. PLoS One, 12(11): e0187277. https://doi.
org/10.1371/journal.pone.0187277
Lee AS, de Lencastre H, Garau J, et al (2018) Methicillin-resistant
Staphylococcus aureus. Nat Rev Dis Primers 4: 18033. https://doi.
org/10.1038/nrdp.2018.33
Lina G, Piémont Y, Godail-Gamot F, et al (1999) Involvement of Panton-
Valentine leukocidin-producing Staphylococcus aureus in primary
skin infections and pneumonia. Clin Infect Dis 29(5): 1128-1132.
Lovseth A, Loncarevic S, Berdal KG (2004) Modified multiplex PCR
method for detection of pyrogenic exotoxin genes in staphylococcal
isolates. J Clin Microbiol 42(8): 3869-3872. https://doi.org/10.1128/
JCM.42.8.3869-3872.2004
Malik S, Peng H, Barton MD (2006) Partial nucleotide sequencing of
the mecA genes of Staphylococcus aureus isolates from cats and
dogs. J Clin Microbiol 44(2): 413-416. https://doi.org/10.1128/
JCM.44.2.413-416.2006
Monday SR, Bohach GA (1999) Use of multiplex PCR to detect classical
and newly described pyrogenic toxin genes in staphylococcal
isolates. J Clin Microbiol 37(10): 3411-3414. https://doi.org/10.1128/
JCM.37.10.3411-3414.1999
Morgan M (2008) Methicillin-resistant Staphylococcus aureus and animals:
zoonosis or humanosis?. J Antimicrob Chemother 62(6): 1181-
Munive Nuñez, KV, d Silva Abreu AC, Gonçalves JL, et al (2023) Virulence
and antimicrobial resistance genes profiles of spa type t605
methicillin-susceptible Staphylococcus aureus isolated from subclinical
bovine mastitis. J Appl Microbiol 134(4). https://doi.org/10.1093/
jambio/lxad057
Paramasivam R, Gopal DR, Dhandapani R, et al (2023) Is AMR in Dairy
Products a Threat to Human Health? An Updated Review on the Origin,
Prevention, Treatment, and Economic Impacts of Subclinical
Mastitis. Infect Drug Resist 16: 155-178. https://doi.org/10.2147/
IDR.S384776
Patel BR, Vagh AA, Vala KB, et al (2023): Prevalence of Subclinical Mastitis
in Gir Cows in and around Junagadh District of Gujarat. Indian
Vet J 19(4): 50-54. https://doi.org/10.48165/ijvsbt.19.4.10
Phophi L, Petzer IM, Qekwana DN, et al (2019) Antimicrobial resistance
patterns and biofilm formation of coagulase-negative Staphylococcus
species isolated from subclinical mastitis cow milk samples submitted
to the Onderstepoort Milk Laboratory. BMC Vet Res 15(1): 420.
Raspanti CG, Bonetto CC, Vissio C, et al (2016) Prevalence and antibiotic
susceptibility of coagulase-negative Staphylococcus species from
bovine subclinical mastitis in dairy herds in the central region of Argentina.
Rev Argent Microbiol 48(1):50-56. https://doi.org/10.1016/j.
ram.2015.12.001
Ren Q, Liao G, Wu Z, et al (2020) Prevalence and characterization of
Staphylococcus aureus isolates from subclinical bovine mastitis in
southern Xinjiang, China. J Dairy Sci 103(4): 3368-3380. https://doi.
org/10.3168/jds.2019-17420
Roshan M, Parmanand Arora D, Behera M, et al (2022) Virulence and enterotoxin
gene profile of methicillin-resistant Staphylococcus aureus
isolates from bovine mastitis. Comp Immunol Microbiol Infect Dis
Rossi BF, Bonsaglia ECR, Castilho IG, et al (2019) Genotyping of
long term persistent Staphylococcus aureus in bovine subclinical
mastitis. Microb Pathog 132, 45-50. https://doi.org/10.1016/j.micpath.
04.031
Saini V, McClure JT, Leger D, et al (2012) Antimicrobial resistance profiles
of common mastitis pathogens on Canadian dairy farms. J Dairy
Sci 95(8): 4319-4332. https://doi.org/10.3168/jds.2012-5373
Singh I, Roshan M, Vats A, et al (2023) Evaluation of Virulence, Antimicrobial
Resistance and Biofilm Forming Potential of Methicillin-
Resistant Staphylococcus aureus (MRSA) Isolates from Bovine
Suspected with Mastitis. Curr Microbiol 80(6): 198. https://doi.
org/10.1007/s00284-023-03303-2
Song X, Huang X, Xu H, et al (2020): The prevalence of pathogens causing
bovine mastitis and their associated risk factors in 15 large dairy
farms in China: An observational study. Vet Microbiol 247, 108757.
Sun M, Gao J, Ali T, et al (2017) Characteristics of Aerococcus viridans
isolated from bovine subclinical mastitis and its effect on milk SCC,
yield, and composition. Trop Anim Health Prod 49(4): 843-849.
Şeker E, Özenç E, Baki A, et al (2019) Prevalence of Methicillin Resistance
and Panton-Valentine Leukocidin Genes in Staphylococci Isolated
from Pirlak Sheep with Subclinical Mastitis in Turkey. Kocatepe
Vet J 1-1. https://doi.org/10.30607/kvj.617025
Ünal N, (2013) Subklinik mastitisli inek sütlerinden izole edilen Staphylococcus aureus izolatlarında bazı toksin genleri ve metisilin direnç
geninin araştırılması. Vet J Ankara Univ, 60, 21-26.
Ünal N, Askar S, Macun HC, et al (2012) Panton-Valentine leukocidin
and some exotoxins of Staphylococcus aureus and antimicrobial
susceptibility profiles of staphylococci isolated from milks of small
ruminants, Trop. Anim. Health. Prod., 44(3), 573-579. https://doi.
org/10.1007/s11250-011-9937-7
Ünal N, Cinar OD, (2012) Detection of stapylococcal enterotoxin, methicillin-
resistant and Panton-Valentine leukocidin genes in coagulase-
negative staphylococci isolated from cows and ewes with subclinical
mastitis. Trop Anim Health Prod 44(2):369-375. https://doi.
org/10.1007/s11250-011-0032-x
Unal S, Hoskins J, Flokowitsch JE, et al (1992) Detection of methicillin-
resistant staphylococci by using the polymerase chain reaction.
J Clin Microbiol 30(7): 1685-1691. https://doi.org/10.1128/
jcm.30.7.1685-1691.1992
Wang SC, Wu CM, Xia SC, et al (2009) Distribution of superantigenic
toxin genes in Staphylococcus aureus isolates from milk samples
of bovine subclinical mastitis cases in two major diary production
regions of China. Vet Microbiol 137(3-4): 276-281. https://doi.
org/10.1016/j.vetmic.2009.01.007
Xu J, Tan X, Zhang X, (2015) The diversities of staphylococcal species,
virulence and antibiotic resistance genes in the subclinical mastitis
milk from a single Chinese cow herd. Microb Pathog 88, 29-38.
Yang F, Shi W, Meng N, et al (2023): Antimicrobial resistance and
virulence profiles of staphylococci isolated from clinical bovine
mastitis. Front microbiol 14:1190790. https://doi.org/10.3389/
fmicb.2023.1190790
Yilmaz M, Şeker E, (2022) Investigation of mecA, vanA and pvl genes in
Staphylococcus aureus strains isolated from bovine mastitis in smallholder
dairy farms. Etlik Vet Mikrobiyol Derg 33(1): 50-55. https://
doi.org/10.35864/evmd.1008728
