Detection of Staphylococcal Enterotoxin, Methicillin-Resistant, Panton- Valentine Leukocidin Genes and Antibiotic Susceptibility in Staphylococci and Current Bacteria Associated with Subclinical Mastitis in Cattle
Аннотация
Bovine mastitis is a significant infectious disease affecting dairy animals, resulting in substantial economic losses. Staphylococci are the predominant causative agents of mastitis. This study aimed to identify the bacteria isolated from cow's milk with subclinical mastitis using MALDI-TOF mass spectrometry, assess their antibiotic susceptibility, and examine the presence of staphylococcal enterotoxin, pvl (Panton-Valentine Leukocidin), and mecA genes in Staphylococcus spp. Out of 301 milk samples, 73 isolates were identified as bacteria, with 40 (54.8%) being Staphylococci. The prevalence of antibiotic resistance among Staphylococcus spp. was as follows: benzylpenicillin 57.5% (n:23), tigecycline 37.5% (n:15), tetracycline 22.5% (n:9), erythromycin 17.5% (n:7), gentamicin 12.5% (n:5), cefoxitin 10.0% (n:4), trimethoprim/sulfamethoxazole 5.0% (n:2), ciprofloxacin 2.5% (n:1), and rifampicin 2.5% (n:1). Among the Staphylococcus haemolyticus isolates, 5 (11.62%) carried the mecA gene, and interestingly, 3 of these were phenotypically sensitive to cefoxitin despite harboring the mecA gene. However, none of the isolates carried the enterotoxin and pvl genes. This study was emphasized that antibiotic resistance of staphylococci isolated from subclinical with mastitis cows raised but these isolates were absent staphylococcal enterotoxin and pvl genes.
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Ünal, N., Şavluk, M., & Kıymacı, M. (2025). Detection of Staphylococcal Enterotoxin, Methicillin-Resistant, Panton- Valentine Leukocidin Genes and Antibiotic Susceptibility in Staphylococci and Current Bacteria Associated with Subclinical Mastitis in Cattle. Journal of the Hellenic Veterinary Medical Society, 75(4), 8363–8370. https://doi.org/10.12681/jhvms.37159
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- Том 75 № 4 (2024)
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- Research Articles
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