test Investigation of Bacteriocin and Probiotic Properties of Lactic Acid Bacteria from Pastrami|Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας

Investigation of Bacteriocin and Probiotic Properties of Lactic Acid Bacteria from Pastrami

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Δημοσιευμένα: Νοε 6, 2025
DOI: https://doi.org/10.12681/jhvms.39177
HB Disli
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Erciyes University, Turkey
Z Gonulalan
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Erciyes University, Turkey
Περίληψη

The aim of this study was to determine the microbiological quality, LAB profile, phylogenetics of Lactobacillaceae and bacteriocin synthesis potential of pastrami isolates produced in Turkey, Kayseri. The probiotic properties of bacteriocinogen strains were investigated in the second stage of the research. To identify the isolates, MALDI-TOF MS and 16S rRNA sequence analysis methods were used. The bacteriocinogenic properties of the strains were evaluated both phenotypically, using the well diffusion method, and at the gene level, using conventional and RT-PCR. To control the stability of cell-free supernatants of bacteriocinogen strains, tolerance tests for enzyme, temperature and pH were performed. To evaluate the probiotic properties of the strains, we investigated their tolerance to gastric juice and bile salts, susceptibility to antibiotics, and ability to synthesize EPSs. The identification process revealed that the dominant species in pastrami was Latilactobacillus sakei. Based on the analyses performed, it was determined that 15 strains (14.56%) exhibited phenotypic antimicrobial activity against at least one of the selected indicator bacteria. Five strains were found to be positive for at least one of the sakacin P and sakacin Q synthesis genes on molecular analysis, which were confirmed by sequence analysis. Cell-free supernatants of bacteriocinogen strains were found to be tolerant of low pH and different temperature conditions. In conclusion, it has been determined that the Latilactobacillus sakei 2 and Latilactobacillus curvatus 48 strains have significant potential in terms of probiotic and starter culture properties.

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Αναφορές
Albedwawi AS, Turner MS, Olaimat AN, Osaili TM, Al-Nabulsi AA,
Liu SQ, Shah NP, Ayyash MM. (2021). An overview of microbial
mitigation strategies for acrylamide: LAB, yeast, and cell-free extracts.
Lebensmittel-Wissenschaft Und Technologie [Food Science
and Technology], 143(111159), 111159. https://doi.org/10.1016/j.
lwt.2021.111159
Castellano P, Pérez Ibarreche, M, Blanco Massani M, Fontana, C,
Vignolo G (2017) Strategies for pathogen biocontrol using LAB
and their metabolites: A focus on meat ecosystems and industrial
environments Microorganisms, 5(3), 38. https://doi.org/10.3390/
microorganisms5030038
Chen C, Chen X, Jiang M, Rui X, Li W, Dong M (2014) A newly discovered
bacteriocin from Weissella hellenica D1501 associated
with Chinese Dong fermented meat (Nanx Wudl). Food Control,
, 116–124. https://doi.org/10.1016/j.foodcont.2014.01.031
Clinical and Laboratory Standards Institute (CLSI) (2022) M100 performance
standards for antimicrobial susceptibility testing, 28th ed.
Cocolin L, Rantsiou K (2007) Sequencing and expression analysis of
sakacin genes in Lactobacillus curvatus strains. Applied Microbiology
and Biotechnology, 76(6), 1403–1411. https://doi.org/10.1007/
s00253-007-1120-8
da Costa RJ, Voloski FLS, Mondadori RG, Duval EH, Fiorentini ÂM
(2019) Preservation of meat products with bacteriocins produced
by LAB isolated from meat. Journal of Food Quality, 2019, 1–12.
https://doi.org/10.1155/2019/4726510
de Souza Barbosa M, Todorov SD, Ivanova I, Chobert JM, Haertlé
T, de Melo Franco BDG (2015) Improving safety of salami by
application of bacteriocins produced by an autochthonous Lactobacillus
curvatus isolate. Food Microbiology, 46, 254–262. https://
doi.org/10.1016/j.fm.2014.08.004
Dec M, Urban-Chmiel R, Gnat S, Puchalski A, Wernicki A (2014)
Identification of Lactobacillus strains of goose origin using MALDI-
TOF mass spectrometry and 16S–23S rDNA intergenic spacer
PCR analysis. Research in Microbiology, 165(3), 190–201. https://
doi.org/10.1016/j.resmic.2014.02.003
Dobson A, Cotter DP, Ross RP, Hill G (2012) Bacteriocin production:
A probiotic trait? Appl. Environ. Microbiol, 78, 1–6. 10.1128/
AEM.05576-11
Ertekin Ö, Kaban G, Kaya M (2023) Genotypic diversity and antagonistic
activities of enterococci isolated from pastırma. Journal
of Food Science and Technology. https://doi.org/10.1007/s13197-
-05895-w
Gontijo MTP, Silva J. de S, Vidigal PMP, Martin JGP (2020) Phylogenetic
distribution of the bacteriocin repertoire of LAB species
associated with artisanal cheese. Food Research International
(Ottawa, Ont.), 128(108783), 108783. https://doi.org/10.1016/j.
foodres.2019.108783
Hsiao E. McBride, S. Hsien, S, Sharon, G, Hyde, E, McCue T, Codelli
J, Chow J, Reisman S, Petrosino J, et al. (2013) Microbiota modulate
behavioral and physiological abnormalities associated with
neurodevelopmental disorders. Cell, 155, 1451–1463. 10.1016/j.
cell.2013.11.024
Janßen D, Dworschak L, Ludwig C, Ehrmann MA, Vogel RF (2020)
Interspecies assertiveness of Lactobacillus curvatus and Lactobacillus
sakei in sausage fermentations. International Journal of Food
Microbiology, 331(108689), 108689. https://doi.org/10.1016/j.ijfoodmicro.
108689
Jeon EB, Son SH, Jeewanthi RKC, Lee NK, Paik HD (2016) Characterization
of Lactobacillus plantarum Lb41, an isolate from kimchi
and its application as a probiotic in cottage cheese. Food Science
and Biotechnology, 25(4), 1129–1133. https://doi.org/10.1007/
s10068-016-0181-9
Lee Y, Cho Y, Kim E, Kim HJ, Kim HY (2018) Identification of LAB
in galchi- and myeolchi-jeotgalby 16S rRNA sequencing, MALDI
TOF mass spectrometry, and PCR-DGGE. Journal of Microbiology
and Biotechnology, 28(7), 1112–1121. https://doi.org/10.4014/
jmb.1803.03034
Ma Q, Fu Y, Sun H, Huang Y, Li L, Yu Q, Dinnyes A, Sun, Q (2017)
Antimicrobial resistance of Lactobacillus spp. from fermented foods
and human gut. Lebensmittel-Wissenschaft Und Technologie [Food
Science and Technology], 86, 201–208. https://doi.org/10.1016/j.
lwt.2017.07.059
Macwana SJ, Muriana PM (2012) A ‘bacteriocin PCR array’ for identification
of bacteriocin-related structural genes in LAB. Journal of
Microbiological Methods, 88(2), 197–204. https://doi.org/10.1016/j.
mimet.2011.11.008
Mathiesen G, Huehne K, Kroeckel L, Axelsson L, Eijsink VGH (2005)
Characterization of a new bacteriocin operon in sakacin P-producing
Lactobacillus sakei, showing strong translational coupling
between the bacteriocin and immunity genes. Applied and Environmental
Microbiology, 71(7), 3565–3574. https://doi.org/10.1128/
aem.71.7.3565-3574.2005
Moradi M, Kousheh SA, Almasi H, Alizadeh A, Guimarães JT, Yılmaz
N, Lotfi A (2020) Postbiotics produced by lactic acid bacteria:
The next frontier in food safety. Comprehensive Reviews
in Food Science and Food Safety, 19(6), 3390–3415. https://doi.
org/10.1111/1541-4337.12613
Novik G, Savich V (2020) Beneficial microbiota. Probiotics and pharmaceutical
products in functional nutrition and medicine. Microbes and
Infection, 22(1), 8–18. https://doi.org/10.1016/j.micinf.2019.06.004
Öz E, Kaban G, Barış Ö, Kaya M (2017) Isolation and identification
of LAB from pastırma. Food Control, 77, 158–162. https://doi.
org/10.1016/j.foodcont.2017.02.017
Özlü H, Atasever M (2019) Bacteriocinogenic bacteria isolated from
Civil, Kashar and White cheeses in Erzurum, Turkey. Ankara
Üniversitesi Veteriner Fakültesi Dergisi, 66(2), 147-153. https://
doi.org/10.33988/auvfd.547483
Prechtl RM, Janßen D, Behr J, Ludwig C, Küster B, Vogel RF, Jakob
F (2018) Sucrose-Induced Proteomic Response and Carbohydrate
Utilization of Lactobacillus sakei TMW 1.411 During Dextran
Formation. Frontiers in Microbiology, 9. https://doi.org/10.3389/
fmicb.2018.02796
Qiao N, Wittouck S, Mattarelli P, Zheng J, Lebeer S, Felis GE, Gänzle
MG (2022) After the storm Perspectives on the taxonomy of Lactobacillaceae.
JDS Communications, 3(3), 222–227. https://doi.
org/10.3168/jdsc.2021-0183
Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh
Jahromi M, Ho YW (2014) Probiotic potential of Lactobacillus
strains with antimicrobial activity against some human pathogenic
strains. BioMed Research International, 2014, 1–16. https://doi.
org/10.1155/2014/927268
Son SH, Jeon HL, Yang SJ, Sim MH, Kim YJ, Lee NK, Paik HD (2018)
Probiotic LAB isolated from traditional Korean fermented foods
based on β-glucosidase activity. Food Science and Biotechnology,
(1), 123–129. https://doi.org/10.1007/s10068-017-0212-1
Stecher G, Tamura K, Kumar S (2020) Molecular evolutionary genetics
analysis (MEGA) for macOS. Molecular Biology and Evolution,
(4), 1237–1239. https://doi.org/10.1093/molbev/msz312
Todorov SD, Rachman C. Fourrier A, Dicks LMT, van Reenen CA, Prévost
H, Dousset X (2011) Characterization of a bacteriocin produced
by Lactobacillus sakei R1333 isolated from smoked salmon. Anaerobe,
(1), 23–31. https://doi.org/10.1016/j.anaerobe.2010.01.004
Todorov SD, Stojanovski S, Iliev I, Moncheva P, Nero LA, Ivanova IV
(2017) Technology and safety assessment for LAB isolated from traditional Bulgarian fermented meat product “lukanka.” Brazilian
Journal of Microbiology, 48(3), 576–586. https://doi.org/10.1016/j.
bjm.2017.02.005
Todorov SD. Vaz-Velho M, de Melo Franco BDG, Holzapfel WH (2013)
Partial characterization of bacteriocins produced by three strains
of Lactobacillus sakei, isolated from salpicao, a fermented meat
product from North-West of Portugal. Food Control, 30(1), 111–121.
https://doi.org/10.1016/j.foodcont.2012.07.022
Topcu KC, Kaya M, Kaban G (2020) Probiotic properties of LAB strains
isolated from pastırma. Lebensmittel-Wissenschaft Und Technologie
[Food Science and Technology], 134(110216), 110216. https://
doi.org/10.1016/j.lwt.2020.110216
Voidarou C, Antoniadou Μ, Rozos G, Tzora A, Skoufos I, Varzakasm
T, Lagiou A, Bezirtzoglou E (2020) Fermentative foods: Microbiology,
biochemistry, potential human health benefits and public
health issues. Foods (Basel, Switzerland), 10(1), 69. https://doi.
org/10.3390/foods10010069
Wang B, Song Q, Zhao F, Zhang L, Han Y, Zhou Z (2019) Isolation
and characterization of dextran produced by Lactobacillus sakei L3
from Hubei sausage. Carbohydrate Polymers, 223(115111), 115111.
https://doi.org/10.1016/j.carbpol.2019.115111
Won SM, Chen S, Lee SY, Lee KE, Park KW, Yoon JH (2020) Lactobacillus
sakei ADM14 induces anti-obesity effects and changes
in gut microbiome in high-fat diet-induced obese mice. Nutrients,
(12), 3703. https://doi.org/10.3390/nu12123703
Xu HQ, Gao L, Jiang YS, Tian Y, Peng J, Xa QQ, Chen Y (2015)
Transcriptome response of Lactobacillus sakei to meat protein environment:
Transcriptome response of Lactobacillus sakei. Journal
of Basic Microbiology, 55(4), 490–499. https://doi.org/10.1002/
jobm.201400540
Yildiran H, Başyi̇ği̇t Kiliç G, Karahan Çakmakçi AG (2019) Characterization
and comparison of yeasts from different sources for some
probiotic properties and exopolysaccharide production. Food Science
and Technology, 39(suppl 2), 646–653. https://doi.org/10.1590/
fst.29818
Zheng J, Wittouck S, Salvetti E, Franz CMAP, Harris HMB, Mattarelli
P, O’Toole PW, Pot B, Vandamme P, Walter J, Watanabe K, Wuyts
S, Felis GE, Gänzle MG, Lebeer S (2020) A taxonomic note on
the genus Lactobacillus: Description of 23 novel genera, emended
description of the genus Lactobacillus Beijerinck 1901, and union
of Lactobacillaceae and Leuconostocaceae. International Journal
of Systematic and Evolutionary Microbiology, 70(4), 2782–2858.
https://doi.org/10.1099/ijsem.0.004107
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