Effects of Proanthocyanidin on Freezability of Ram Sperm
Περίληψη
This study aimed to investigate the effect of different concentrations of proanthocyanidin (PA) added to the semen diluent of Sönmez rams on semen quality. Ejaculates were gathered from three rams once a week with an artificial vagina. The ejaculates were separated into five groups with diluents without (control, C) and containing (10, 25, 50, and 100 µg/ml) antioxidants. Samples were equilibrated for 3 hours at 5 0C and then frozen in liquid nitrogen vapor. The increase in motility and the decrease in the head, tail, and total abnormal spermatozoa rates in the 10 µg/ml PA group were statistically important (p<0.05) compared to the C. The increase in H+/E- was statistically important (p<0.05) in the group 10 µg/ml PA compared to the C group. The decrease in tail length, tail DNA in the groups with 10, 25 µg/ml PA added, and the decrease in tail moment in all antioxidant-added groups was statistically important (p<0.001) compared to the C. The increase in TOS was statistically significant (p<0.05) in the groups with 10, 25, and 100 µg/ml PA added compared to the C. In conclusion, it was observed that the 10 µg/ml dose of PA added to the semen provided the best protection on semen quality compared to other groups in storing ram semen.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
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Avdatek, F., & Berk, S. (2025). Effects of Proanthocyanidin on Freezability of Ram Sperm. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 76(3), 9721–9728. https://doi.org/10.12681/jhvms.39931
- Τεύχος
- Τόμ. 76 Αρ. 3 (2025)
- Ενότητα
- Research Articles
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Λήψεις
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Αναφορές
Aitken R J, Jones K T, Robertson S A (2012) Reactive oxygen species
and sperm function-in sickness and in health. Journal of Andrology,
, 1096-1106.
Al-Daraji H J (2012) The use of certain grape constituents for improve
semen quality and storage ability of diluted Roosters’ semen. Am
J Pharm Tech Res., 2, 308-322.
Attia S M, Bakheet S A, Al-Rasheed N M (2010) Proanthocyanidins
produce significant attenuation of doxorubicin-induced mutagenicity
via suppression of oxidative stress. Oxid Med Cell Longev, 3,
-413.
Avdatek F, Birdane Y O, Türkmen R, Demirel H H (2018) Ameliorative
effect of resveratrol on testicular oxidative stress, spermatological
parameters and DNA damage in glyphosate-based herbicide-exposed
rats. Andrologia, 50(7), e13036.
Avdatek F, Gündoğan M (2018) Effects of some antioxidant additives on
spermatological parameters, oxidative stress and DNA damage after
freezing-thawing process in ram semen. Sağlık Bilimleri Veteriner
Dergisi, Fırat Üniversitesi, 32, 135-142.
Avdatek F, Yeni D, Taşdemir U (2020) Influence of proanthocyanidin
on motility and osmotic resistance parameters of merino ram sperm
during short term storage. Kocatepe Veterinary Journal, 13, 362-367.
Avdatek F, İnanç ME, Gülhan MF, Güngör Ş, Yeni D, Olğaç KT, Denk
B, Taşdemir U (2023). Investigation of the effects of syringic acid
supplemented to Tris semen diluent on ram semen freezability.
Reproduction in Domestic Animals, 58(7), 997-1004.
Bagchi D, Bagchi M, Stohs S J, Das D K, Ray, S D, Kuszynski C A,
Pruess H G (2000) Free radicals and grape seed proanthocyanidin
extract: importance in human health and disease prevention. Toxicology,
, 187-197.
Bai H, Wang Z, Cui J, Yun K, Zhang H, Liu R H, Cheng C (2014)
Synergistic radiation protective effect of purified Auricularia auricular-
judae polysaccharide (AAP IV) with grape seed procyanidins.
Molecules, 19, 20675-20694.
Bashir N, Shagirtha K, Manoharan V, Miltonprabu S (2019) The molecular
and biochemical insight view of grape seed proanthocyanidins
in ameliorating cadmium-induced testes-toxicity in rat
model: implication of PI3K/Akt/Nrf-2 signaling. Biosci Rep., 39
(1), BSR20180515.
Bucak M N, Tekin N (2006) Protective effect of taurine, glutathione
and trehalose on the liquid storage of ram semen. Small Ruminant
Res., 73, 103-108.
Draper H H, Hadley M (1990) Malondialdehyde determination as index
of lipid peroxidation. Methods Enzymol., 186, 421-430.
Ellıot J G (1999) Application of antioxidant vitamins in foods and
beverages. Food Tech., 53, 46-48.
Evans G, Maxwell W M C (1987) Collection of semen; Handling and
examination of semen; Dilution of semen; Frozen storage of semen;
Insemination. Salmon’s Artificial Insemination of Sheep and Goats,
-166, Butterworths, Sydney, Australia.
Fine A M (2000) Oligomeric proanthocyanidin complexes: history,
structure, and phytopharmaceutical applications. Alter Med Rev.,
, 144-151.
Fracassetti D, Costa C, Moulay L, Tomás-Barberán F A (2013) Ellagic
acid derivatives, ellagitannins, proanthocyanidins and other phenolics,
vitamin C and antioxidant capacity of two powder products
from camu-camu fruit (Myrciaria dubia). Food Chemistry., 139,
-588.
Fraczek M, Szkutnik D, Sanocka D, Kurpisz M (2001) Peroxidation
components of sperm lipid membranes in male infertility. Ginekologia
Polska., 72, 73-79.
Güngör Ş, İnanç M E, Ata A (2019) Effect of gallic acid on ram semen
spermatological parameters at +4 °C storage. Eurasian Journal of
Veterinary Sciences., 35, 87-92.
Hala A H, Khattab Z A, Abdallah G, Kamel M (2010) Grape seed extract
alleviate reproductive toxicity caused by aluminium chloride in
male rats. J Am Sci., 6, 352-361.
He L, Li P, Yu L H, Li L, Zhang Y, Guo Y, Yang S H (2018) Protective
effects of proanthocyanidins against cadmium-induced testicular
injury through the modification of Nrf2-Keap1 signal path in
rats. Environmental Toxicology and Pharmacology., 57, 1-8.
Inanç M E, Çil B, Yeni D, Avdatek F, Orakçı D, Tuncer P B, Türkmen
R, Taşdemir U (2019) The effect of green teaextract supplementation
in bull semen cryopreservation.Kafkas Üniv Vet Fak Derg.,
, 703-708.
Li Q, Shaoyong W, Li Y, Chen M, Hu Y, Liu B, Hu J (2018) Effects of
oligomeric proanthocyanidins on quality of boar semen during liquid
preservation at 17 C. Animal Reproduction Science., 198, 47-56.
Long M Yang S, Zhang Y, Li P, Han J, Dong S, He J (2017) Proanthocyanidin
protects against acute zearalenone-induced testicular oxidative
damage in male mice. Environmental Science and Pollution
Research, 24, 938-946.
Lü Song Jie L, Wu Cai Feng WC, Fan Wen Hua FW, Zhang Shu Shan
ZS, Dai Jian Jun DJ, Sun Ling Wei SL, Zhang De Fu ZD (2019)
Effect of procyanidins addition on antioxidant ability of Hu sheep
freezing semen. Acta Agriculturae Shanghai, 35(3), 95-99.
Medeiros C M O, Forell F, Oliveira A T D, Rodrigues J L (2002) Current
status of sperm cryopreservation: why isn’t it better?. Theriogenology.,
, 327-344.
Mu CT, Yang WJ, Wang PJ, Ren GD, Ju H, Ren YS, Hao XY, Zhang J X
(2020) Effects of grape seed procyanidins on growth performance,
semen quality, antioxidant indexes in testis and epididymis of ram.
Chinese Journal of Animal Nutrition, 32(5), 2241-2250.
Nie Y, Stürzenbaum S R (2019) Proanthocyanidins of natural origin:
molecular mechanisms and implications for lipid disorder and aging-
associated diseases. Advances in Nutrition., 10, 464-478.
Qamar AY, Naveed MI, Raza S, Fang X, Roy PK, Bang S, Tanga BM,
Saadeldin IM, Lee S, Cho J (2022) Role of antioxidants in fertility
preservation of sperm—A narrative review. Animal bioscience,
(3), 385.
Ruane J (2000). A framework for prioritizing domestic animal breeds
for conservation purposes at the national level: a Norwegian case
study. Conservation Biology., 14, 1385-1393.
Salami S A, Guinguina A, Agboola J O, Omede A A, Agbonlahor E M,
Tayyab U (2016) In vivo and postmortem effects of feed antioxidants
in livestock: a review of the implications on authorization of
antioxidant feed additives. Animal., 10, 1375-1390.
Salamon S, Maxwell W M C (1995) Frozen storage of ram semen I.
Processing, freezing, thawing and fertility after cervical insemination.
Animal Reproduction Science., 37, 185-249. I
Sedlak J, Lindsay R H C (1968) Estimation of total, protein bound and
nonprotein sulfhydryl groups in tissue with Ellmann’s reagent. Anal
Biochem., 25, 192-205.
Sönmez M F, Tascioglu S (2016) Protective effects of grape seed extract
on cadmium-induced testicular damage, apoptosis, and endothelial
nitric oxide synthases expression in rats. Toxicol Ind Health., 32,
-1494.
Su L, Deng Y, Zhang Y, Li C, Zhang R, Sun Y, Yao S (2011) Protective
effects of grape seed procyanidin extract against nickel sulfate-induced
apoptosis and oxidative stress in rat testes. Toxicology Mechanisms
and Methods., 21, 487-494.
Taşdemir U, Yeni D, İnanç M E, Avdatek F, Çil B, Türkmen R (2020)
Red pine (Pinus brutia Ten) bark tree extract preserves sperm quality
by reducing oxidative stress and preventing chromatin damage.
Andrologia., 52, 1-7.
Tuncer P B, Bucak M N, Büyükleblebici S, Sarıözkan S, Yeni D, Eken A,
Gündoğan M (2010) The effect of cysteine and glutathione on sperm
and oxidative stress parameters of post-thawed bull semen. Cryobiology.,
, 303-307.
Türk G (2015) Reaktif oksijen türlerinin spermatozoon fonksiyonları
üzerindeki fizyolojik ve patolojik etkileri. Turkiye Klinikleri J Reprod
Artif Insemin-Special Topics., 1, 26-34.
Watson P F (1975) Use of a Giemsa stain to detect changes in acrosomes
of frozen ram spermatozoa. Veterinary Record., 97, 12-15.
Wen F, Li Y, Feng T, Du Y, Ren F, Zhang Li, Hu J (2019) Grape seed
procyanidin extract (GSPE) improves goat sperm quality when
preserved at 4 C. Animals., 9, 810.
Wood J E, Senthilmohan S T, Peskin A V (2002) Antioxidant activity of
procyanidin-containing plant extracts at di_erent phs. Food Chem.,
, 155-161.
Zhao J, Jin Y, Du M, Liu W, Ren Y, Zhang C, Zhang J (2017) The effect
of dietary grape pomace supplementation on epididymal sperm
quality and testicular antioxidant ability in ram lambs. Theriogenology.,
, 50-56.
Zhao Y M, Gao L P, Zhang H L, Guo J X, Guo P P (2014) Grape seed
proanthocyanidin extract prevents DDP-induced testicular toxicity
in rats. Food Function, 5, 605-611.
