test Effects of Proanthocyanidin on Freezability of Ram Sperm|Journal of the Hellenic Veterinary Medical Society

Effects of Proanthocyanidin on Freezability of Ram Sperm

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Publicado: Nov 7, 2025
DOI: https://doi.org/10.12681/jhvms.39931
F Avdatek
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
https://orcid.org/0000-0003-2345-8826
SB Berk
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
Resumen

This study aimed to investigate the effect of different concentrations of proanthocyanidin (PA) added to the semen diluent of Sönmez rams on semen quality. Ejaculates were gathered from three rams once a week with an artificial vagina. The ejaculates were separated into five groups with diluents without (control, C) and containing (10, 25, 50, and 100 µg/ml) antioxidants. Samples were equilibrated for 3 hours at 5 0C and then frozen in liquid nitrogen vapor. The increase in motility and the decrease in the head, tail, and total abnormal spermatozoa rates in the 10 µg/ml PA group were statistically important (p<0.05) compared to the C. The increase in H+/E- was statistically important (p<0.05) in the group 10 µg/ml PA compared to the C group. The decrease in tail length, tail DNA in the groups with 10, 25 µg/ml PA added, and the decrease in tail moment in all antioxidant-added groups was statistically important (p<0.001) compared to the C. The increase in TOS was statistically significant (p<0.05) in the groups with 10, 25, and 100 µg/ml PA added compared to the C. In conclusion, it was observed that the 10 µg/ml dose of PA added to the semen provided the best protection on semen quality compared to other groups in storing ram semen.

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Biografía del autor/a
F Avdatek, Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey

Department of Reproduction and Artificial Insemination

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