Feeding with heat-killed Gordonia bronchialis affects growth performance, intestinal morphology and immunomodulation in Japanese quail (Coturnix coturnix japonica)

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DOI: https://doi.org/10.12681/jhvms.27522
Keywords:
Japanese quail Growth Newcastle disease Interferon Gordonia bronchialis
K. NOFOUZI
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
M. ZEHTABNAJAFI
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
M. KHORDADMEHR
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
M.S. MADADI
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
V.R. RANJBAR JAMALABADI
Clinic of poultry disease, Yazd Province, Yazd, Iran
Abstract

Studies have shown that bacterial immunomodulators based on aerobic Actinomycetales such can switch off pre-existing Th2 preponderance and prompt Th1-mediated mechanisms, whatever the previous environmental immune priming of the individual. Gordonia bronchialis (G. bronchialis) is a Gram-positive, weakly acid-fast species of the genus Gordonia within the sub-order Corynebacteriaceae of the order Actinomycetales. In this study, the efficacy of heat-killed Gordonia bronchialis (G. bronchialis) on growth performance, immune system and intestinal structure in quail (Corurnix coturnix japonica) was evaluated. Quails (mean weight 7.8 g) were fed basal diet (control), or treatment diets containing 105 (low dose) or 106 (high dose) CFU per bird per day in food of heat-killed G. Bronchialis continuously (for 42 days). Body weight gain (BWG) and feed consumption was recorded during grower period and finisher period. On days 7, 14, 28, 35 and 42 some of the quails were sampled for analysis of Newcastle antibody titer.Interleukin-4 (IL-4), Interferon- α (IFN-α), and interferon- γ (IFN-γ) concentrations were analyzed using ELISA kits. An indirect ELISA was performed to quantifying IgA. At the end of 14, 28 and 42 days old, three chicks from each group were selected for histopathological and histomorphometrical studies. Results showed that growth performance was significantly enhanced in both treatment groups compared with the control group. Serum anti Newcastle disease virus, IL-4 and IFN-α titers were higher in low dose treatment group compared with the control group. The length of the intestinal and pyloric caeca folds was increased in the high-dose group. Meanwhile, jejunum and ileum showed the most significant morphological changes in different days of sampling, particularly in high dose group. Among the evaluated factors, villous length and intestinal crypt depth demonstrated more significant differences. This study suggests that heat-killed G. bronchialis has the potential to enhance growth, immunological parameters and the intestinal structure in Japanese quail.

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