Influence of lavender essential oil (Lavandula angustifolia) and oxytetracycline in nutrition of honey bees, prevention of American foulbrood and overall welfare

PDF (English)
Publicado: Apr 29, 2022
DOI: https://doi.org/10.12681/jhvms.25747
Nikola Puvača
University Business Academy in Novi Sad, Faculty of Economics and Engineering Management in Novi Sad, Department of Engineering Management in Biotechnology
https://orcid.org/0000-0002-5500-7010
Resumen
Recently, there has been an increasing demand for natural, healthy, and safe products without residual antibiotics for human consumption, particularly bee products. Beekeepers have been struggling with this problem many years, having in mind often occurrence of American foulbrood, which is one of the most severe honey bee brood diseases, and till now have been successfully eradicated with heavy usage of antibiotics. Such controlled, or mostly uncontrolled usage of antibiotics in fighting against American foulbrood lead to a residual quantity of antibiotic in honey. To overcome this problem, this research aimed to investigate the influence of single essential oil (Lavandula angustifolia) compared to antibiotics in honey bee production on the prevention of American foulbrood. Totally three treatments were formed artificially infected with P. larvae spore suspension, at concentration 2×109 spore/ml. The course of the disease was regularly monitored. Treatment one (T1) did not receive antibiotic therapy. Treatment two (T2) was given lavender essential oil at a concentration of 0.1% of sugar syrup. The treatment was applied for 30 days, at 48h intervals. Treatment three (T3) received antibiotics in the sugar syrup at a concentration of 0.1%, respectively. Clinical and laboratory examinations were performed on days 10, 20, 40 and 60, respectively. Besides, L. angustifolia essential oil rich in Ethanol, 2-(2-ethoxyethoxy)- (13.05%), linalool (10.71%), α-Terpinyl acetate (10.93%) and linalool acetate (9.60%), showed its positive effects against antibiotics in combat of American foulbrood, further research with a specifically designed qualitative and quantitative mixture of essential oils are more than necessary because single essential oil is not enough and didn’t show expected results.
Article Details
  • Sección
  • Research Articles
Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Descargas
Los datos de descargas todavía no están disponibles.
Biografía del autor/a
Nikola Puvača, University Business Academy in Novi Sad, Faculty of Economics and Engineering Management in Novi Sad, Department of Engineering Management in Biotechnology

Department of Engineering Management in Biotechnology

Associate Professor and Research Associate

Citas
Ács, K., Balázs, V. L., Kocsis, B., Bencsik, T., Böszörményi, A., & Horváth, G. (2018). Antibacterial activity evaluation of selected essential oils in liquid and vapor phase on respiratory tract pathogens. BMC Complementary and Alternative Medicine, 18(1), 227. https://doi.org/10.1186/s12906-018-2291-9
Albo, G. N., Henning, C., Ringuelet, J., Reynaldi, F. J., De Giusti, M. R., & Alippi, A. M. (2003). Evaluation of some essential oils for the control and prevention of American Foulbrood disease in honey bees. Apidologie, 34(5), 417–427. https://doi.org/10.1051/apido:2003040
Al-Waili, N., Salom, K., Al-Ghamdi, A., & Ansari, M. J. (2012). Antibiotic, Pesticide, and Microbial Contaminants of Honey: Human Health Hazards. The Scientific World Journal, 2012, 1–9. https://doi.org/10.1100/2012/930849
Antúnez, K., Anido, M., Arredondo, D., Evans, J. D., & Zunino, P. (2011). Paenibacillus larvae enolase as a virulence factor in honeybee larvae infection. Veterinary Microbiology, 147(1–2), 83–89. https://doi.org/10.1016/j.vetmic.2010.06.004
Bogdanov, S. (2006). Contaminants of bee products. Apidologie, 37(1), 1–18. https://doi.org/10.1051/apido:2005043
Carson, C. F., & Riley, T. V. (1995). Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. Journal of Applied Bacteriology, 78(3), 264–269. https://doi.org/10.1111/j.1365-2672.1995.tb05025.x
Copping, L. G., & Duke, S. O. (2007). Natural products that have been used commercially as crop protection agents. Pest Management Science, 63(6), 524–554. https://doi.org/10.1002/ps.1378
Cristina, R., Kovačević, Z., Cincović, M., Dumitrescu, E., Muselin, F., Imre, K., Militaru, D., Mederle, N., Radulov, I., Hădărugă, N., & Puvača, N. (2020). Composition of a Natural Phytotherapeutic Association Beneficially Used to Combat Nosemosis in Honey Bees. Sustainability, in press.
Cristina, R. T., Mederle, N., Soreanu, M., Moruzi, R. F., Dumitrescu, E., Muselin, F., Dar, A. P., Imre, K., & Militaru, D. (2020). THE EFFICIENCY OF A PHYTOTHERAPEUTIC ASSOCIATION TO COMBAT NOSEMOSIS IN HONEY BEES IN ROMANIA. Revista Romana de Medicina Veterinara, 30(1), 13–18.
Da Porto, C., Decorti, D., & Kikic, I. (2009). Flavour compounds of Lavandula angustifolia L. to use in food manufacturing: Comparison of three different extraction methods. Food Chemistry, 112(4), 1072–1078. https://doi.org/10.1016/j.foodchem.2008.07.015
de Graaf, D. C., Alippi, A. M., Antúnez, K., Aronstein, K. A., Budge, G., De Koker, D., De Smet, L., Dingman, D. W., Evans, J. D., Foster, L. J., Fünfhaus, A., Garcia-Gonzalez, E., Gregore, A., Human, H., Murray, K. D., Nguyen, B. K., Poppinga, L., Spivak, M., van Engelsdorp, D., … Genersch, E. (2013). Standard methods for American foulbrood research. Journal of Apicultural Research, 52(1), 1–28. https://doi.org/10.3896/IBRA.1.52.1.11
Durand, C., & Fournier, S. (2017). Can Geographical Indications Modernize Indonesian and Vietnamese Agriculture? Analyzing the Role of National and Local Governments and Producers’ Strategies. World Development, 98, 93–104. https://doi.org/10.1016/j.worlddev.2015.11.022
Easton-Calabria, A., Demary, K. C., & Oner, N. J. (2019). Beyond Pollination: Honey Bees (Apis mellifera) as Zootherapy Keystone Species. Frontiers in Ecology and Evolution, 6, 161. https://doi.org/10.3389/fevo.2018.00161
Ebert, T. A., Kevan, P. G., Bishop, B. L., Kevan, S. D., & Downer, R. A. (2007). Oral toxicity of essential oils and organic acids fed to honey bees ( Apis mellifera ). Journal of Apicultural Research, 46(4), 220–224. https://doi.org/10.1080/00218839.2007.11101398
Eteraf-Oskouei, T., & Najafi, M. (2013). Traditional and modern uses of natural honey in human diseases: A review. Iranian Journal of Basic Medical Sciences, 16(6), 731–742.
Facchini, E., Bijma, P., Pagnacco, G., Rizzi, R., & Brascamp, E. W. (2019). Hygienic behaviour in honeybees: A comparison of two recording methods and estimation of genetic parameters. Apidologie, 50(2), 163–172. https://doi.org/10.1007/s13592-018-0627-6
Forsgren, E. (2010). European foulbrood in honey bees. Journal of Invertebrate Pathology, 103, S5–S9. https://doi.org/10.1016/j.jip.2009.06.016
Fuselli, S. R., de la Rosa, S. B. G., Gende, L. B., Eguaras, M. J., & Fritz, R. (2006). Antimicrobial activity of some Argentinean wild plant essential oils against Paenibacillus larvae larvae, causal agent of American foulbrood (AFB). Journal of Apicultural Research, 45(1), 2–7. https://doi.org/10.1080/00218839.2006.11101304
Genersch, E. (2008). Paenibacillus larvae and American Foulbrood – long since known and still surprising. Journal Für Verbraucherschutz Und Lebensmittelsicherheit, 3(4), 429–434. https://doi.org/10.1007/s00003-008-0379-8
Genersch, Elke. (2007). Paenibacillus larvae and American foulbrood in honeybees. Berliner Und Munchener Tierarztliche Wochenschrift, 120(1–2), 26–33.
González-Rivera, J., Duce, C., Falconieri, D., Ferrari, C., Ghezzi, L., Piras, A., & Tine, M. R. (2016). Coaxial microwave assisted hydrodistillation of essential oils from five different herbs (lavender, rosemary, sage, fennel seeds and clove buds): Chemical composition and thermal analysis. Innovative Food Science & Emerging Technologies, 33, 308–318. https://doi.org/10.1016/j.ifset.2015.12.011
Hansen, H., & Brødsgaard, C. J. (1999). American foulbrood: A review of its biology, diagnosis and control. Bee World, 80(1), 5–23. https://doi.org/10.1080/0005772X.1999.11099415
Hau-Yama, N. E., Magaña-Ortiz, D., Oliva, A. I., & Ortiz-Vázquez, E. (2020). Antifungal activity of honey from stingless bee Melipona beecheii against Candida albicans. Journal of Apicultural Research, 59(1), 12–18. https://doi.org/10.1080/00218839.2019.1665247
Hitokoto, H., Morozumi, S., Wauke, T., Sakai, S., & Kurata, H. (1980). Inhibitory effects of spices on growth and toxin production of toxigenic fungi. Applied and Environmental Microbiology, 39(4), 818–822.
Ignjatijević, S., Prodanović, R., Bošković, J., Puvača, N., Tomaš-Simin, M., Peulić, T., & Đuragić, O. (2019). Comparative analysis of honey consumption in Romania, Italy and Serbia. Food and Feed Research, 46(1), 125–136. https://doi.org/10.5937/FFR1901125I
Jenkins, R. (2016). A review of selected bee products as potential anti-bacterial, anti-fungal, and anti-viral agents. Medical Research Archives, 4(8). https://doi.org/10.18103/mra.v4i8.887
Kevan, P. G., & Menzel, R. (2012). The plight of pollination and the interface of neurobiology, ecology and food security. The Environmentalist, 32(3), 300–310. https://doi.org/10.1007/s10669-012-9394-5
Khan, S. U., Anjum, S. I., Ansari, M. J., Khan, M. H. U., Kamal, S., Rahman, K., Shoaib, M., Man, S., Khan, A. J., Khan, S. U., & Khan, D. (2019). Antimicrobial potentials of medicinal plant’s extract and their derived silver nanoparticles: A focus on honey bee pathogen. Saudi Journal of Biological Sciences, 26(7), 1815–1834. https://doi.org/10.1016/j.sjbs.2018.02.010
Kieliszek, M., Piwowarek, K., Kot, A. M., Błażejak, S., Chlebowska-Śmigiel, A., & Wolska, I. (2018). Pollen and bee bread as new health-oriented products: A review. Trends in Food Science & Technology, 71, 170–180. https://doi.org/10.1016/j.tifs.2017.10.021
Koulivand, P. H., Khaleghi Ghadiri, M., & Gorji, A. (2013). Lavender and the Nervous System. Evidence-Based Complementary and Alternative Medicine, 2013, 1–10. https://doi.org/10.1155/2013/681304
Kütükoğlu, F., Gi̇Ri̇Şgi̇N, A. O., & Aydin, L. (2012). Varroacidal efficacies of essential oils extracted from Lavandula officinalis, Foeniculum vulgare, and Laurus nobilis in naturally infested honeybee (Apis mellifera L.) colonies. Turk. J. Vet. Anim. Sci, 36(5), 554–559.
Kuzyšinová, K., Mudroňová, D., Toporčák, J., Molnár, L., & Javorský, P. (2016). The use of probiotics, essential oils and fatty acids in the control of American foulbrood and other bee diseases. Journal of Apicultural Research, 55(5), 386–395. https://doi.org/10.1080/00218839.2016.1252067
Laird, K., & Phillips, C. (2012). Vapour phase: A potential future use for essential oils as antimicrobials?: Essential oil vapours and their antimicrobial activity. Letters in Applied Microbiology, 54(3), 169–174. https://doi.org/10.1111/j.1472-765X.2011.03190.x
López-Romero, D., Izquierdo-Vega, J., Morales-González, J., Madrigal-Bujaidar, E., Chamorro-Cevallos, G., Sánchez-Gutiérrez, M., Betanzos-Cabrera, G., Alvarez-Gonzalez, I., Morales-González, Á., & Madrigal-Santillán, E. (2018). Evidence of Some Natural Products with Antigenotoxic Effects. Part 2: Plants, Vegetables, and Natural Resin. Nutrients, 10(12), 1954. https://doi.org/10.3390/nu10121954
Nizar, H., Alaa, A., Noureddine, A., Fares, K., & Quddoumi, S. (2015). Diagnosis of Paenibacillus larvae from Honeybees in Jordan According to Microbiological and Chemicals Techniques. Asian Journal of Animal Sciences, 9(6), 318–329. https://doi.org/10.3923/ajas.2015.318.329
Nozal, M. J., Bernal, J. L., Jiménez, J. J., González, M. J., & Higes, M. (2002). Extraction of thymol, eucalyptol, menthol, and camphor residues from honey and beeswax. Journal of Chromatography A, 954(1–2), 207–215. https://doi.org/10.1016/S0021-9673(02)00153-X
OIE - World Organisation for Animal Health (Ed.). (2008). Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). 2: Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees) (6. ed). OIE.
Özkök, D., & Akyol, E. (2017). The Effects of Some Drugs Used to Treat Honeybee (Apis mellifera L.) Diseases and Pests on Lifespan of Honeybees. Turkish Journal of Agriculture - Food Science and Technology, 5(7), 720. https://doi.org/10.24925/turjaf.v5i7.720-723.1092
Plavša, N., Stojanović, D., Stojanov, I., Puvača, N., Stanaćev, V., & Đuričić, B. (2011). Evaluation of oxytetracycline in the prevention of American foulbrood in bee colonies. African Journal of Agricultural Research, 6(6), 1621–1626. https://doi.org/10.5897/AJAR10.1060
Prodanović, R., Ignjatijević, S., & Bošković, J. (2019). Innovative potential of beekeeping production in AP Vojvodina. Journal of Agronomy, Technology and Engineering Management, 2(3), 268–277.
Puvača, N., Čabarkapa, I., Petrović, A., Bursić, V., Prodanović, R., Soleša, D., & Lević, J. (2019). Tea tree (Melaleuca alternifolia ) and its essential oil: Antimicrobial, antioxidant and acaricidal effects in poultry production. World’s Poultry Science Journal, 75(2), 235–246. https://doi.org/10.1017/S0043933919000229
Puvača, N., Stanaćev, V., Glamočić, D., Lević, J., Perić, L., Stanaćev, V., & Milić, D. (2013). Beneficial effects of phytoadditives in broiler nutrition. World’s Poultry Science Journal, 69(1), 27–34. https://doi.org/10.1017/S0043933913000032
Puvača, Nikola, Lika, E., Tufarelli, V., Bursić, V., Ljubojević Pelić, D., Nikolova, N., Petrović, A., Prodanović, R., Vuković, G., Lević, J., & Giannenas, I. (2020). Influence of different tetracycline antimicrobial therapy of Mycoplasma (Mycoplasma synoviae) in laying hens compared to tea tree essential oil on table egg quality and antibiotics residues. Foods, 9(5), 612. https://doi.org/10.3390/foods9050612
Roller, S., Ernest, N., & Buckle, J. (2009). The Antimicrobial Activity of High-Necrodane and Other Lavender Oils on Methicillin-Sensitive and -Resistant Staphylococcus aureus (MSSA and MRSA). The Journal of Alternative and Complementary Medicine, 15(3), 275–279. https://doi.org/10.1089/acm.2008.0268
Roussenova, N. (2011). Antibacterial activity of essential oils against the etiological agent of American foulbrood disease (Paenibacillus larvae). Bulgarian Journal of Veterinary Medicine, 14(1), 17–24.
Roxana Nicoleta, L., & Silvia, P. (2020). Use of Essential Oils in Bees. Scientific Papers: Animal Science & Biotechnologies, 53(1), 74–79.
Sammataro, D., Finley, J., LeBlanc, B., Wardell, G., Ahumada-Segura, F., & Carroll, M. J. (2009). Feeding essential oils and 2-heptanone in sugar syrup and liquid protein diets to honey bees ( Apis mellifera L.) as potential Varroa mite ( Varroa destructor ) controls. Journal of Apicultural Research, 48(4), 256–262. https://doi.org/10.3896/IBRA.1.48.4.05
Sinha, R. K. (1997). Embarking on the Second Green Revolution for Sustainable Agriculture in India: A Judicious Mix of Traditional Wisdom and Modern Knowledge in Ecological Farming. Journal of Agricultural and Environmental Ethics, 10(2), 183–197. https://doi.org/10.1023/A:1007796609378
Spivak, M., & Downey, D. L. (1998). Field Assays for Hygienic Behavior in Honey Bees (Hymenoptera: Apidae). Journal of Economic Entomology, 91(1), 64–70. https://doi.org/10.1093/jee/91.1.64
Srivastava, A., Srivastava, P., Pandey, A., Khanna, V. K., & Pant, A. B. (2019). Phytomedicine. In New Look to Phytomedicine (pp. 625–655). Elsevier. https://doi.org/10.1016/B978-0-12-814619-4.00025-2
Sturtevant, A. P., & Revell, I. L. (1953). Reduction of Bacillus Larvae Spores in Liquid Food of Honey Bees by Action of the Honey Stopper, and Its Relation to the Development of American Foulbrood. Journal of Economic Entomology, 46(5), 855–860. https://doi.org/10.1093/jee/46.5.855
Tagboto, S., & Townson, S. (2001). Antiparasitic properties of medicinal plants and other naturally occurring products. In Advances in Parasitology (Vol. 50, pp. 199–295). Elsevier. https://doi.org/10.1016/S0065-308X(01)50032-9
Thompson, H. M., Waite, R. J., Wilkins, S., Brown, M. A., Bigwood, T., Shaw, M., Ridgway, C., & Sharman, M. (2005). Effects of European foulbrood treatment regime on oxytetracycline levels in honey extracted from treated honeybee ( Apis mellifera ) colonies and toxicity to brood. Food Additives and Contaminants, 22(6), 573–578. https://doi.org/10.1080/02652030500089986
van den Heever, J. P., Thompson, T. S., Curtis, J. M., Ibrahim, A., & Pernal, S. F. (2014). Fumagillin: An Overview of Recent Scientific Advances and Their Significance for Apiculture. Journal of Agricultural and Food Chemistry, 62(13), 2728–2737. https://doi.org/10.1021/jf4055374
Vapa-Tankosić, J., Ignjatijević, S., Kiurski, J., Milenković, J., & Milojević, I. (2020). Analysis of Consumers’ Willingness to Pay for Organic and Local Honey in Serbia. Sustainability, 12(11), 4686. https://doi.org/10.3390/su12114686
Wiese, N., Fischer, J., Heidler, J., Lewkowski, O., Degenhardt, J., & Erler, S. (2018). The terpenes of leaves, pollen, and nectar of thyme (Thymus vulgaris) inhibit growth of bee disease-associated microbes. Scientific Reports, 8(1), 14634. https://doi.org/10.1038/s41598-018-32849-6
Yoshiyama, M., & Kimura, K. (2009). Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood. Journal of Invertebrate Pathology, 102(2), 91–96. https://doi.org/10.1016/j.jip.2009.07.005
Živanović, S., Pavlović, D., Stojanović, N., & Veljković, M. (2019). Attitudes to and prevalence of bee product usage in pediatric pulmonology patients. European Journal of Integrative Medicine, 27, 1–6. https://doi.org/10.1016/j.eujim.2019.02.001
Artículos más leídos del mismo autor/a