Dynamic changes of psychrotrophic bacterial populations in Algerian refrigerated raw cow milk

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DOI: https://doi.org/10.12681/jhvms.26764
K BOUCHAIR
Laboratory of Natural Sciences and Materials, University Centre Abdelhafid Boussouf, Mila, Algeria
A BOUBENDIR
Laboratory of Natural Sciences and Materials, University Centre Abdelhafid Boussouf, Mila, Algeria
A SERRER
Laboratory of Natural Sciences and Materials, University Centre Abdelhafid Boussouf, Mila, Algeria
Resumen

The aim of the present study is to evaluate the microbial dynamic changes in raw cow milk during refrigeration. Milk samples were collected from two dairy farms situated in basin and mountain areas in the region of Mila, Algeria. Cold storage of milk samples at 4 °C was used to promote psychrotrophic microbial growth during 10 days. The microbial flora of milk was determined by culture methods and the identification of bacterial isolates was carried using cultural, morphological and biochemical criteria. The variations in total viable count and kinetic parameters of microbial growth were analyzed during refrigeration using Baranyi and Roberts Model. The initial microbial counts ranged from 2.94 ±0.04 to 3.87 ±0.11 log CFU * mL-1 and the maximum growth did not exceed 7.00 ±0.12 log CFU * mL-1. The hygienic limit was surpassed after 2 days in the basin farm and later after 6 days in mountain farm. The generation time varied from 1.20 ±0.55 to 4.18 ±0.22 days in the basin farm and mountain farm respectively. The psychrotrophic bacterial populations were identified as Acinetobacter, Pseudomonas, Aeromonas, Chryseobacterium, Enterococcus and Lactobacillus. The notable delay in microbial growth in milk from the mountain farm compared to the one from basin could be related to indoor/outdoor feeding mode adopted in mountain opposed to indoor feeding in the basin farm. This prolonged shelf-life suggests the possible presence of antimicrobial molecules coming from plants grazed around the farm and the selection in the microbiota of some microbial species with antagonist potential.

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Ananda Baskaran S, Kazmer GW, Hinckley L, Andrew SM, and Venkitanarayanan K (2009) Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro. J Dairy Sci 92:1423-1429.
Baranyi J, Roberts TA (1995) Mathematics of predictive food microbiology. Int J Food Microbiol 26: 199-218.
Bekker A (2011) Growth and spoilage characteristics of Chryseobacterium species in milk. Magister Scientiae thesis -Food Microbiology University of the Free State, South Africa.
Boubendir A, Hamidechi MA, Mostakim M, El Abed S, Ibnsouda S, Koraich I (2011) Incidence of Listeria spp. and other psychrotrophic bacteria in raw bovine milk in the North East of Algeria. Rev Med Vet-Toulouse 162: 265-269.
Boubendir A, Serrazanetti DI, Hamidechi MA, Vannini L, Guerzoni ME (2016) Changes in bacterial populations in refrigerated raw milk collected from a semi-arid area of Algeria. Ann Microbiol 66: 777-783.
Cempírková R, Mikulová M (2009) Incidence of psychrotrophic lipolytic bacteria in cow’s raw milk. Czech J Anim Sci 54: 65-73.
Citak S, Yucel N, Mendi A (2005) Antibiotic resistance of enterococcal isolates in raw milk. J Food Process Pres 29: 183-195.
Darsanaki RK, Aliabadi MA, Rokhi ML, Nourbakhsh M, Raeisi G (2012) Antimicrobial activity of olive leaf aqueous extract. Ann Biol Res 3: 4189-4191.
Decimo M, Morandi S, Silvetti T, Brasca M (2014) Characterization of gram-negative psychrotrophic bacteria isolated from Italian bulk tank milk. J Food Sci 79: 2081-2090.
De Garnica ML, Santos JA, Gonzalo C (2011) Influence of storage and preservation on microbiological quality of silo ovine milk. J Dairy Sci 94: 1922-1927.
De Jonghe V, Coorevits A, Van Hoorde K, Messens W, Van Landschoot A, De Vos P, Heyndrickx M (2011) Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Appl Environ Microbiol 77: 460-470.
FAO (2013) Milk and Dairy Products in Human Nutrition. Food and Agricultural Organization of the United Nations, Rome, Italy.
Franciosi E, De Sabbata G, Gardini F, Cavazza A, Poznanski E (2011) Changes in psychrotrophic microbial populations during milk creaming to produce Grana Trentino cheese. Food Microbiol 28: 43-51.
Franz CM, van Belkum MJ, Holzapfel WH, Abriouel H, Gálvez A (2007) Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol Rev 31: 293-310.
Gargouri A, Hamed H, Elfeki A (2013) Analysis of raw milk quality at reception and during cold storage: combined effects of somatic cell counts and psychrotrophic bacteria on lipolysis. J Food Sci 78:M1405-M1411.
Gaysinsky S, Taylor TM, Davidson PM, Bruce BD, Weiss J (2007) Antimicrobial efficacy of eugenol microemulsions in milk against Listeria monocytogenes and Escherichia coli O157:H7. J Food Protect 70:231-237.
Giannino ML, Marzotto M, Dellaglio F, Feligini M (2009) Study of microbial diversity in raw milk and fresh curd used for Fontina cheese production by culture-independent methods. Int J Food Microbiol 130:188-195.
Gislene B, Favarin L, Luchese RH, Mcintosh D (2015) Psychrotrophic bacteria in milk: How much do we really know?. Braz J Microbiol 46:313-321.
Gleeson D, O’connell A, Jordan K (2013) Review of potential sources and control of thermoduric bacteria in bulk tank milk. Irish J Agr Food Res 52: 217-227.
Gutiérrez-Cortés C, Suárez H, Buitrago G, Moreno CD (2017) Isolation and evaluation of the antagonist activity of lactic acid bacteria in raw cow milk. Agronomía Colombiana 35: 357-364.
Hantsis-Zacharov E, Halpern M (2007a) Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. Appl Environ Microbiol 73: 7162-7168.
Hantsis-Zacharov E, Halpern M (2007b) Chryseobacterium haifense sp. nov., a psychrotolerant bacterium isolated from raw milk. Int J Syst Evol Microbiol 57: 2344-2348.
Higginbotham KL, Burris K, Zivanovic S, Davidson PM, Stewart CN (2013) Antimicrobial activity of Hibiscus sabdariffa aqueous extracts against Escherichia coli O157:H7 and Staphylococcus aureus in a microbiological medium and milk of various fat concentrations. J Food Protect 77: 262-268.
Lafarge V, Ogier JC, Girard V, Maladen V, Leveau JY, Gruss A, Delacroix-Buchet A (2004) Raw cow milk bacterial population shifts attributable to refrigeration. Appl Environ Microbiol 70: 5644-5650.
Larsen MK, Hymøller L, Brask-Pedersen DB, Weisbjerg MR (2012) Milk fatty acid composition and production performance of Danish Holstein and Danish Jersey cows fed different amounts of linseed and rapeseed. J Dairy Sci 95: 3569-3578.
Lim CS, Oh YS, Lee JK, Park AR, Yoo JS, Rhee SK, Roh DH (2011) Flavobacterium chungbukense sp. nov., isolated from soil. Int J Syst Evol Microbiol 61: 2734-2739.
Lopez MJ, NICHOLS NN, DIEN BS, MORENO J, BOTHAST RJ (2004) Isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates. Appl Microbiol Biotechnol 64: 125-131.
Machado SG, Bazzolli DMS, Vanetti MCD (2013) Development of a PCR method for detecting proteolytic psychrotrophic bacteria in raw milk. Int Dairy J 29: 8-14.
Machado SG, DA Silvf LA, Bazzolli DMS, Heyndrickx M, Costa PM, Vanetti MCD (2015) Pseudomonas spp. and Serratia liquefaciens as Predominant Spoilers in Cold Raw Milk. J Food Sci 80: M1842-M1849.
Machado SG, Baglinière F, Marchand S, Van coillie E, Vanetti MCD, De block J, Heyndrickx M (2017) The Biodiversity of the Microbiota Producing Heat-Resistant Enzymes Responsible for Spoilage in Processed Bovine Milk and Dairy Products. Front Microbiol 8: 1-22.
Mami A, Hamedi AR, Henni J, Kerfouf A, Kihal M (2012) Screening of autochthonous Lactobacillus species from Algerian raw goats’ milk for the production of bacteriocin-like compounds against Staphylococcus aureus. Afr J Biotechnol 11: 4595-4607.
Mankai M, Boulares M, Ben Moussa O, Karoui R, Hassouna M (2012) The effect of refrigerated storage of raw milk on the physicochemical and microbiological quality of Tunisian semihard Gouda-type cheese during ripening. Int J Dairy Technol 65: 250-259.
Mcauley CM, Britz ML, Gobius KS, Craven HM (2015) Prevalence seasonality and growth of Enterococci in raw and pasteurized milk in Victoria, Australia. J Dairy Sci 98: 8348-8358.
Michel V, Hauwuy A, CHAMBA JF (2001) Raw cow milk microflora: diversity and influence of conditions of production. Dairy Sci Technol 81: 575-592.
Morandi S, Cremonesi P, Povolo M, Brasca M (2012) Enterococcus lactis sp. nov from Italian raw milk cheeses. Int J Syst Evol Microbiol 62:1992-1996.
Nahla TK (2006) Study on mesophilic Aeromonas in milk and some milk products in Port Said city. J Appl Sci Res 11: 1037-1041.
O’connell A, Ruegg PL, Jordan K, O’brien B, Gleeson D (2016) The effect of storage temperature and duration on the microbial quality of bulk tank milk. J Dairy Sci 99: 3367-3374.
Ouelbani R, Bensari S, Mouas TN, Khelifi D (2016) Ethnobotanical investigations on plants used in folk medicine in the regions of Constantine and Mila (North-East of Algeria). J Ethno Pharm 194: 196-218.
Paludetti LF, Jordan K, Kelly AL, Gleeson D (2018) Evaluating the effect of storage conditions on milk microbiological quality and composition. Irish J Agr Food Res 57: 52-62.
Paşca C, Mărghitaș LA, Dezmirean D, Bobiș O, Bonta V, Mărgăoan R, Chirilă F, Fit N (2015) The assessment of the antibacterial activity of some plant extracts on normal and pathogenic microflora from milk. Anim Sci Biotechnol 48: 166-172.
Perko B (2011) Microbiological quality of raw milk. Mljekarstvo 61: 114-124.
Quigley L, O’sullivan O, Stanton C, Beresford TP, Ross RP, Fitzgerald GF, Cotter PD (2013) The complex microbiota of raw milk. J Dairy Sci 96: 4928-4937.
Rasolofo EA, St-gelais D, Lapointe G, Roy D (2010) Molecular analysis of bacterial population structure and dynamics during cold storage of untreated and treated milk. Int J Food Microbiol. 138: 108-118.
Ribeiro Júnior JC, De oliveira AM, Silva FG, Tamanini R, De oliveira ALM, Beloti V (2018) The main spoilage-related psychrotrophic bacteria in refrigerated raw milk. J Dairy Sci 101: 75-83.
Samaržija D, Zamberlin Š, Pogacic T (2012) Psychrotrophic bacteria and milk quality. Mljekarstvo 62: 77-95.
Sanz Sampelayo MR, Chilliard Y , Schmidely P, Boza J (2007) Influence of type of diet on the fat constituents of goat and sheep milk. Small Ruminant Res 68: 42-63.
Sharifi Rad J, Lalanne GM, Alvarez AJH, Taheri Y, SHaheen SH, Kregiel D, Antolak H, Pawlikowska E, Jokanovic MB, Rajkovic J, Hosseinabadi T, Masic BL, Baghalpour N, Mohajeri M, Martins N (2020) Malva species: Insights on its chemical composition towards pharmacological applications. Phytother Res 34: 546-567.
Silvetti T, Morandi S, Brasca M (2014) Biopreservation potential of Enterococcus faecalis isolated from Italian traditional raw milk cheeses. J Food 12: 210-217.
Toqeer A, Kanwal R, Avub N (2006) Influence of temperature on growth pattern of Lactococcus lactis, Streptococcus cermoris and Lactobacillus acidophilus isolated from camel milk. J Biotechnol 5: 481-488.
Vithanage NR, Yeager TR, Jadhav SR, Palombo EA, Datta N (2014) Comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk. Int J Food Microbiol 189: 26-38.
Vithanage NR, Dissanayake M, Bolge G, Palombo EA, Yeager TR, Datta N (2017) Microbiological quality of raw milk attributable to prolonged refrigeration conditions. J Dairy Res 84: 92-101.
Vyletělova M, Hanuš O, Urbanova E, Kopunecz P (2000) The occurrence and identification of psychrotrophic bacteria with proteolytic and lipolytic activity in bulk milk samples at storage in primary production conditions. Czech J Anim Sci 45: 373-383.
Yabrir B, Zobiri A, Laoun A, Titouche Y, Chenouf NS, Ranebi D, Isselnane S, Mati A (2018) Bacteriological behavior of ewe’s raw milk collected in Algerian area steppe and refrigerated at 4°C or 7°C. Livestock Res Rural Dev 30(2). http://www.lrrd.org/lrrd30/2/byab30023.html
Yuan L, Sadiq FA, Liu TJ, LI Y, GU J, Yang HY (2018) Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes. J Zhejiang Univ-SC B 19: 630-642.