Vaginal cytology during the estrous cycle and early pregnancy of ewes


M Haq’qi Qobadi
M Goli
https://orcid.org/0000-0003-4393-6493
T Mohammadi
H Cheraghi
Resumen

Changes in vaginal epithelial cells and blood progesterone and estrogen concentrations were compared between estrous cycle and early pregnancy in multiparous Sanjabee ewes. Twenty non-pregnant ewes were synchronized with intravaginal insertion of a controlled internal drug release (CIDR) device which was in place for 7 days, intramuscular administration of a GnRH analog (Alarelin acetate; 12.5 µg) at the time of CIDR insertion (Day -7), a PGF2α analog (D-cloprostenol sodium; 125 µg) and 500 I.U. of human chorionic gonadotropin (hCG) at the time of CIDR removal (Day 0). At Day 0, all ewes were introduced to four fertile rams and observed for estrous signs. Ewes exhibiting estrous signs were divided into two groups: MATED (confirmed pregnant with transrectal ultrasound scanning at Day 35) and NOT-MATED (confirmed non-pregnant with transrectal ultrasound scanning at Day 35). Vaginal smears and blood samples were taken daily and every other day, respectively, from the beginning of estrus for 20 days for cytology and hormone assay. Based on the results, during days 0 and 1 of the estrous cycle, only the percentages of intermediate cells differed between the groups (P < 0.05). During days 2 to 4, there was no difference in the cell populations between the groups (P ≥ 0.05). During days 5 to 16, the percentages of all cell types except the parabasal cells differed between the groups (P < 0.05). During days 17 to 20, the percentages of all types of vaginal epithelial cells differed between the groups (P < 0.05). Progesterone concentrations increased gradually from days 0 to 14 of the estrous cycle in both groups, however, they decreased significantly afterward in the NOT-MATED group. Estrogen concentrations changes showed an opposite pattern to that of progesterone in the study groups. Collectively, vaginal cytology can be used as a useful tool in assessing hormonal and physiological characteristics of the female reproductive system and thus provides a more accurate understanding of the physiology of estrous cycle and early pregnancy in ewes, which can be used to improve reproductive management.

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Biografía del autor/a
M Haq’qi Qobadi, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

A graduated DVM student

M Goli, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

Department of Clinical Science; Assistant professor

T Mohammadi, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

an assistant professor, Department of Basic Science

H Cheraghi, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

An assistant professor, Department of Clinical Science

Citas
Barrett DMW, Duggavathi R, Davies KL, Bartlewski PM, Bagu ET,
Rawlings NC (2007) Differential Effects of Various Estradiol-17beta
Treatments on Follicle-Stimulating Hormone Peaks, Luteinizing Hormone Pulses, Basal Gonadotropin Concentrations, and Antral Follicle
and Luteal Development in Cyclic Ewes. Biology of Reproduction
: 252-262. doi: 10.1095/biolreprod.106.058842
Bartlewski PM, Beard AP, Rawlings NC (1999) An ultrasonographic study of luteal function in breeds of sheep with different ovulation rates. Theriogenology 52 (1): 115-130. doi: 10.1016/S0093-
X(99)00114-4
Cabrera C, Maier GU, Cuneo M, McNabb BR (2019) The use of progesterone intravaginal devices is superior to use of the ram effect at
hastening the reproductive performance in transitional Targhee ewes.
Davies KL, Bartlewski PM, Pierson RA, Rawlings NC (2006) Computer
assisted image analyses of corpora lutea in relation to peripheral concentrations of progesterone: A comparison between breeds of sheep
with different ovulation rates. Animal Reproduction Science 96 (1-2):
-175. doi: 10.1016/j.anireprosci.2005.12.003
England GWC (1992) Vaginal cytology and cervicovaginal mucus arborisation in the breeding management of bitches. Journal of Small Animal Practice 33: 577-582. https://doi.org/10.1111/j.1748-5827.1992.
tb01060.x
Leigh OO, Raheem AK, Olugbuyiro JAO (2010) Improving the reproductive efficiency of the goat: Vaginal cytology and vulvar biometry
as predictors of synchronized estrus/breeding time in West African
dwarf goat. International Journal of Morphology 28 (3): 923-928. doi:
4067/S0717-95022010000300042
Leigh O, Raji L, Diakodue E (2013) Detection of standing heat in bitches:
Application of vaginal cytology. World Journal of Life Science and
Medical Research 3: 21-25.
Mayor P, Galvez H, Guimaraes DA, Lopez-Gatius F, Lopez-Bejar M
(2007) Serum estradiol-17β, vaginal cytology and vulval appearance
as predictors of estrus cyclicity in the female collared peccary (Tayassu tajacu) from the eastern Amazon region. Animal Reproduction
Science 97 (1-2): 165-174. doi: 10.1016/j.anireprosci.2005.12.017
Mingoas JPK, Ngayam LL (2009) Preliminary findings on vaginal epithelial cells and body temperature changes during oestrous cycle in
Bororo zebu cow. International Journal of Biological and Chemical
Sciences 3 (1): 147-51. doi: 10.4314/ijbcs.v3i1.42745
Mota-Rojas D, Alonso-Spilsbury M, Mayagoitia L, Trujillo ME, Valencia J (2005) Changes of vaginal epithelium in creole pigs ovulating during lactation. Brazilian Journal of Veterinary Research and
Animal Science 42 (5): 333-338. doi: 10.11606/issn.1678-4456.
bjvras.2005.26409
Ola SI, Sanni WA, Egbunike G (2006) Exfoliative vaginal cytology
during the oestrous cycle of West African dwarf goats. Reproduction
Nutrition Development 46 (1): 87-95. doi: 10.1051/rnd:2005067
Pérez-Martı́
nez M, Mendoza ME, Romano MC (1999) Exfoliative vaginal
cytology and plasma levels of estrone and estradiol-17β in young and
adult goats. Small Ruminant Research 33 (2): 153-158. doi: 10.1016/
S0921-4488(99)00008-5
Ribeiro CV, Neves TA, Fagundes GB, do Nascimento DM, da Silva CM,
Arrivabene M, Dias FE, Cavalcante TV (2019) Morphological characterization of vaginal epithelial cells of santainês ewes subjected
to estrus synchronization. Comunicata Scientiae 10 (1): 5-9. doi:
14295/CS.v10i1.2756
Sharma M, Sharma N (2016) Vaginal cytology: an historical perspective
on its diagnostic use. Advances in Animal and Veterinary Sciences 4
(6): 283-288. doi: 10.14737/journal.aavs/2016/4.6.283.288
Siregar TN, Melia J, Thasmi CN, Masyitha D, Wahyuni S, Rosa J, Panjaitan B (2016) Determining proportion of exfoliative vaginal cell
during various stages of estrus cycle using vaginal cytology techniques in aceh cattle. Veterinary Medicine International 3976125. doi:
1155/2016/3976125
Sitaresmi PI, Astuti PK, Widyobroto BP, Bintara S, Widayati DT (2018)
Exfoliative vaginal cytology and vaginal acidity profile in Ettawa-Saanen grade does. International Journal of Pure and Applied
Mathematics 118 (24): 1-16.
Zarkawi M, Soukouti A (2001) Serum progesterone levels using radioimmunoassay during oestrous cycle of indigenous Damascus does.
New Zealand Journal of Agricultural Research 44 (2-3): 165-169.
doi: 10.1080/00288233.2001.9513473
Zohara BF, Azizunnesa, Islam F, Alam GS, Bari FY (2014a) Exfoliative
vaginal cytology and serum progesterone during the estrous cycle of
indigenous ewes in Bangladesh. Journal of Embryo Transfer 29 (2):
-188. doi: 10.12750/JET.2014.29.2.188
Zohara BF, Azizunneasa, Islam F, Alam GS, Bari FY (2014b) Comparison of estrus synchronization by PGF2α and progestagen sponge
with PMSG in indigenous ewes in Bangladesh. GSTF International Journal of Veterinary Science (JVet) 1 (1): 27-37. doi: 10.13140/
RG.2.2.34174.41282