Effect of water-based nutritional supplement on growth performance, carcass characteristics, serum biochemistry, and immunological response of broiler chicken
Resumen
Broiler lines are currently rigorously selected for faster growth and greater final body weight. Nutrition has a critical role in maintaining broiler body growth. This study aimed to determine how water-based nutritional supplements affected broiler chickens' growth performance, carcass characteristics, serum biochemistry, and immunological response. A total of 200 newly hatched unsexed broiler chicks (Cobb-500) were divided into four treatment groups (T1, T2, T3, and T4), replicated five times with ten birds in each, under a completely randomized design. Different concentrations of the supplement, 0% (T1), 2.5% (T2), 5% (T3), and 7.5% (T4), were added to the water. Growth performance, carcass characteristics, serum biochemical profile, and immune antibody response parameters were evaluated for a span of 35 days. The findings demonstrated that body weight gain and feed efficiency significantly improved in the birds provided nutritional supplements at 5 and 7.5% than in the 2.5% and control group (T1). The 5% and 7.5% nutritional supplement groups displayed lower mortality rates compared to other two groups. However, feed consumption did not significantly differ between treatments. The birds fed nutritional supplement at 5 and 7.5% showed enhanced (P<0.05) carcass yield than other groups whereas all other metrics, such as breast yield, thigh yield, wing weight, liver weight, heart weight, gizzard weight, and abdominal fat weight, were not significantly different (P>0.05) between treatments. There were no differences in albumin, total protein, globulin, cholesterol, uric acid, triglycerides, or glucose levels in serum between the various treatments. In comparison to the other groups, the birds administered nutritional supplements at 5% and 7.5% had higher immune antibody titers against the Newcastle disease and infectious bronchitis vaccinations. In conclusion, adding 5% of a nutritional supplement to the water may improve broiler overall performance while being more cost-effective.
Article Details
- Cómo citar
-
Khan, M., Afzal, Z., Arslan, M., Asad, T., Nisa, Q., Akhtar, R., Siddique, B., Nazir, S., Farooq, Z., Wadood, F., Younas, U., Salam, M., Khaliq, H., & Arshad, M. (2025). Effect of water-based nutritional supplement on growth performance, carcass characteristics, serum biochemistry, and immunological response of broiler chicken. Journal of the Hellenic Veterinary Medical Society, 76(2), 9209–9216. https://doi.org/10.12681/jhvms.38657
- Número
- Vol. 76 Núm. 2 (2025)
- Sección
- Research Articles
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Descargas
Los datos de descargas todavía no están disponibles.
Citas
Abdulameer, Y. S., & Alwan, I. A. (2022). Improvement of Growth performance,
Biochemical Blood Profiles, and Meat Peroxidation by the Inclusion of
Mustard Seed Extract in Broilers’ Drinking Water. Archives of Razi Institute,
(1), 429-437.
Adegbeye, M. J., Asaniyan, E. K., Igbalajobi, O. A., Oyedele, D. S., Elghandour,
M. M. M. Y., Salem, A. Z. M., & Falade, T. T. (2019). Influence of
selected plant seeds on the performance, carcass characteristics, sensory
evaluation, and economics of broiler chicken. Tropical Animal Health and
Production, 52(3), 1005-1012.
Adegbeye, M. J., Oloruntola, O. D., Asaniyan, E. K., Agunbiade, B., Oisagah,
E. A., & Ayodele, S. O. (2020). Pawpaw, black cumin, and mustard seed
meals dietary supplementation in broiler chickens: effect on performance,
gut microflora, and gut morphology. Journal of Agricultural Science and
Technology, 22(5), 1235-1246.
Ahmad, Z., Xie, M., Wu, Y., & Hou, S. (2019). Effect of supplemental cyanocobalamin
on the growth performance and hematological indicators of the
white pekin ducks from hatch to day 21. Animals, 9(9), 633.
Ahmed, M. E., & Abbas, T. E. (2011). Effects of dietary levels of methionine
on broiler performance and carcass characteristics. International Journal
of Poultry Science, 10(2), 147–151.
Alagawany, M., El-Hack, A., Mohamed, E., Farag, M. R., Sachan, S., Karthik,
K., & Dhama, K. (2018). The use of probiotics as eco-friendly alternatives
for antibiotics in poultry nutrition. Environmental Science and Pollution
Research, 25(11), 10611–10618.
Anwar, U., Ahmad, S., Abdelgayed, S. S., Hussain, M., Rehman, A., Riaz, M.,
Yousaf, M., Bilal, M. Q., Bhatti, S. A., & Rahman, M. A. (2022). Influence
of phytase with or without organic acid (Sodium Di-Formate) supplementation
on growth performance, carcass response, protein and mineral digestibility
in starter phase of broilers. Brazilian Journal of Poultry Science, 24.
Bolu, S. A., & Balogun, O. O. (2009). Effects of improved (addition of antimicrobials
and antioxidants) locally produced natural vitamin premix on
performance, hematology and some serum constituents of broiler chickens.
Nigerian Journal of Animal Production, 36(2), 246–255.
Carmen, R. P., & Mountney, J. G. (1998). Poultry Meat and Eggs Production.
Corzo, A., Kidd, M. T., & Kerr, B. J. (2003). Threonine need of growing female
broilers1, 2. International Journal of Poultry Science, 2(6), 367–371.
del Barrio, A. S., Mansilla, W. D., Navarro-Villa, A., Mica, J. H., Smeets, J. H.,
Den Hartog, L. A., & García-Ruiz, A. I. (2020). Effect of mineral and vitamin
C mix on growth performance and blood corticosterone concentrations
in heat-stressed broilers. Journal of Applied Poultry Research, 29(1), 23-33.
Deyhim, F., Stoecker, B. S., Adeleye, B. G., & Teeter, R. G. (1995). The effects
of heat distress environment, vitamin, and trace mineral supplementation on performance, blood constituents, and tissue mineral concentrations in
broiler chickens. Nutrition Research, 15(4), 521–526.
Elham, M., Azhar, K., Seyed, R. H., Tech, C., Mohd, H. B., & Homa, D. (2010).
The effect of methionine and threonine supplementations on immune responses
of broiler chickens challenged with infectious bursal disease.
American Journal of Applied Sciences, 7(1), 44–50.
Elnesr, S. S., Alagawany, M., Elwan, H. A. M., Fathi, M. A., & Farag, M. R.
(2020). Sodium butyrate improves the intestinal health of poultry. Annals
of Animal Science, 20(1), 1–13.
Elnesr, S. S., Ropy, A., & Abdel-Razik, A. H. (2019). Effect of dietary sodium
butyrate supplementation on growth, blood biochemistry, haematology
and histomorphometry of intestine and immune organs of Japanese quail.
Animal, 13(6), 1234–1244.
Faluyi, O. B., Agbede, J. O., & Adebayo, I. A. (2015). Growth performance
and immunological response to Newcastle disease vaccinations of broiler
chickens fed lysine supplemented diets. Journal of Veterinary Medicine
and Animal Health, 7(3), 77-84.
Fayyaz, A., Saleemi, M. K., Gul, S. T., Gilani, M. M., & Irshad, H. (2023).
Sero-epidemiology and Pathology of Infectious Bronchitis in Commercial
Poultry from Faisalabad Division. Pakistan Veterinary Journal, 43(1),
-152.
Ghoreyshi, S. M., Omri, B., Chalghoumi, R., Bouyeh, M., Seidavi, A., Dadashbeiki,
M., Lucarini, M., Durazzo, A., van den Hoven, R., & Santini,
A. (2019). Effects of dietary supplementation of L-carnitine and excess
lysine-methionine on growth performance, carcass characteristics, and
immunity markers of broiler chicken. Animals, 9(6), 362.
Grioui, N., Boukhris, H., Damergi, C., Hajji, W., Riahi, H., Abderrabba, M., ...
& Mejri, M. (2021). Dried tomato pomace in rabbit nutrition: effects on
carcass characteristics and meat quality. Turkish Journal of Veterinary &
Animal Sciences, 45(2), 281-287.
Horváth, M., & Babinszky, L. (2018). Impact of selected antioxidant vitamins
(Vitamin A, E and C) and micro minerals (Zn, Se) on the antioxidant
status and performance under high environmental temperature in poultry.
A review. Acta Agriculturae Scandinavica, Section A—Animal Science,
(3), 152–160.
Islam, M. S., Bhuiyan, M. E. R., Begum, M. I. A., Miah, M. A., & Myenuddin,
M. J. B. J. (2004). Effects of vitamin-mineral premix supplementation on
body weight and certain haemato-biochemical values in broiler chickens.
Bangladesh Journal of Veterinary Medicine, 2(1), 45-48.
Karavolias, J., Salois, M. J., Baker, K. T., & Watkins, K. (2018). Raised without
antibiotics: impact on animal welfare and implications for food policy.
Translational Animal Science, 2(4), 337–348.
Khan, M. I., Anjum, F. M., Sohaib, M., & Sameen, A. (2013). Tackling metabolic
syndrome by functional foods. Reviews in Endocrine and Metabolic
Disorders, 3(14), 287-297.
Khan, M. M., Khose, K. K., Manwar, S. J., Gole, M. A., Ali, S. S., & Siddiqui,
M. F. (2018). Effect of L-threonine supplementation in diets on immune
response and carcass traits in broiler chickens.
Khan, M. T., Mehmood, S., Mahmud, A., & Javed, K. (2019). Performance traits,
blood biochemistry, immune response and economic appraisal of broilers
fed different levels of poultry byproducts compost. Journal of Animal &
Plant Sciences, 29(6), 1549-1557.
Khatun, A., Chowdhury, S. Das, Roy, B. C., Dey, B., Haque, A., & Chandran, B.
(2019). Comparative effects of inorganic and three forms of organic trace
minerals on growth performance, carcass traits, immunity, and profitability
of broilers. Journal of Advanced Veterinary and Animal Research, 6(1), 66.
Kidd, M. T., & Kerr, B. J. (1996). L-threonine for poultry: A review. Journal
of Applied Poultry Research, 5(4), 358–367.
Kidd, M. T., McDaniel, C. D., Branton, S. L., Miller, E. R., Boren, B. B., &
Fancher, B. I. (2004). Increasing amino acid density improves live performance
and carcass yields of commercial broilers. Journal of Applied
Poultry Research, 13(4), 593–604.
Kogut, M. H. (2009). Impact of nutrition on the innate immune response to
infection in poultry. Journal of applied poultry research, 18(1), 111-124.
Lee, D.-N., Wu, F.-Y., Cheng, Y.-H., Lin, R.-S., & Wu, P.-C. (2003). Effects
of dietary chromium picolinate supplementation on growth performance
and immune responses of broilers. Asian-Australasian Journal of Animal
Sciences, 16(2), 227–233.
Leeson, S., & Summers, J. D. (1999). Feeding systems for poultry. Feeding
Systems and Feed Evaluation Models., 211–237.
Li, M., Gao, Y., Lan, G., & Gu, Z. (2014). Effects of ultraviolet-B radiation on
immunity and carcass characteristics in quail. Journal of Applied Poultry
Research, 23(3), 429-436.
Lima, H. J. D., Barreto, S. L. T., Donzele, J. L., Souza, G. S., Almeida, R. L.,
Tinoco, I. F. F., & Albino, L. F. T. (2016). Digestible lysine requirement
for growing Japanese quails. Journal of Applied Poultry Research, 25(4),
-491.
Lutful Kabir, S. M. (2009). The role of probiotics in the poultry industry. International
Journal of Molecular Sciences, 10(8), 3531–3546.
Moghaddam, H. S., & Emadi, M. (2014). The effect of threonine and vitamin A
on immune system in broiler chickens. International Journal of Advanced
Biological and Biomedical Research 26, 756–763.
Narinc, D., Aksoy, T., Onenc, A., & Curek, D. I. (2015). The Influence of Body
Weight on Carcass and Carcass Part Yields, and Some Meat Quality Traits
in Fast-and Slow-Growing Broiler Chickens. Kafkas Üniversitesi Veteriner
Fakültesi Dergisi, 21(4), 527-534.
National Research Council (NRC) (1994). Nutrient requirements of poultry. 9th
Rev. ed. Natl. Acad. Press, Washington, DC.
Nollet, L., Van der Klis, J. D., Lensing, M., & Spring, P. (2007). The effect of
replacing inorganic with organic trace minerals in broiler diets on productive
performance and mineral excretion. Journal of Applied Poultry
Research, 16(4), 592–597.
Ojabo, L. D., Oluremi, O. I. A., Carew, S. N., & Uza, D. V. (2013). Heamatology
and serum biochemistry of pullet grower chickens fed sweet orange (Citrus
sinensis) fruit peel meal based diets. Res Opin Anim Vet Sci. 3(8):252–256.
Petracci, M., & Cavani, C. (2011). Muscle growth and poultry meat quality
issues. Nutrients, 4(1), 1–12.
Raza, F. K., Khan, S. A., Raza, A., Saeedand, M. A., & Bashir, I. N. (1997).
Effect of vitamin E deficiency and excess on immune system of broiler
chickens. International Journal of Animal Sciences, 12, 39–41.
Rehman, M. S., Mahmud, A., Mehmood, S., Pasha, T. N., Hussain, J., & Khan,
M. T. (2017). Blood biochemistry and immune response in Aseel chicken
under free range, semi-intensive, and confinement rearing systems. Poultry
Science, 96(1), 226–233.
Rinttilä, T., & Apajalahti, J. (2013). Intestinal microbiota and metabolites—Implications
for broiler chicken health and performance. Journal of Applied
Poultry Research, 22(3), 647–658.
Ripon, M. R., Rashid, H., Rahman, M., Ferdous, F., Arefin, S., Sani, A. A.,
... & Rafiq, K. (2019). Dose-dependent response to phytobiotic supplementation
in feed on growth, hematology, intestinal pH, and gut bacterial
load in broiler chicken. Journal of Advanced Veterinary and Animal Research,
(2), 253-259.
SAS Institute Inc. 2002-03. SAS/STAT User’s Guide: Statistics. Version 9.1.
SAS Inst. Inc., Cary, NC.
Sugiharto, S. (2016). Role of nutraceuticals in gut health and growth performance
of poultry. Journal of the Saudi Society of Agricultural Sciences,
(2), 99-111.
Swain, B. K., Johri, T. S., & Majumdar, S. (2000). Effect of supplementation of
vitamin E, selenium and their different combinations on the performance
and immune response of broilers. British Poultry Science, 41(3), 287–292.
Tarhyel, R., Hena, S. A., & Tanimomo, B. K. (2012). Effects of age on organ
weight and carcass characteristics of Japanese quail (Coturnix japonica).
Scientific Journal of Agricultural, 1(1), 21–26.
Toghyani, M., Toghyani, M., Gheisari, A., Ghalamkari, G., & Mohammadrezaei,
M. (2010). Growth performance, serum biochemistry and blood hematology
of broiler chicks fed different levels of black seed (Nigella sativa)
and peppermint (Mentha piperita). Livestock Science, 129(1–3), 173–178.
Upadhaya, S. D., Lee, B. R., & Kim, I. H. (2016). Effects of ionised or chelated
water-soluble mineral mixture supplementation on growth performance,
nutrient digestibility, blood characteristics, meat quality and intestinal microbiota
in broilers. British Poultry Science, 57(2), 251–256.
Upadhayay, U., & Vishwa, P. C. V. (2014). Growth promoters and novel feed
additives improving poultry production and health, bioactive principles
and beneficial applications: the trends and advances-a review. International
Journal of Pharmacology, 10(3), 129–159.
Wang, J., Yue, H., Wu, S., Zhang, H., & Qi, G. (2017). Nutritional modulation
of health, egg quality and environmental pollution of the layers. Animal
Nutrition, 3(2), 91–96.
Whitehead, C. C. (2002). Nutrition and poultry welfare. World’s Poultry Science
Journal, 58(3), 349-356.
Yadav, S., & Jha, R. (2019). Strategies to modulate the intestinal microbiota
and their effects on nutrient utilization, performance, and health of poultry.
Journal of Animal Science and Biotechnology, 10(1), 1–11.
Yilmaz, E., & Gul, M. (2023). Effects of cumin (Cuminum cyminum L.) essential
oil and chronic heat stress on growth performance, carcass characteristics,
serum biochemistry, antioxidant enzyme activity, and intestinal
microbiology in broiler chickens. Veterinary Research Communications,
(2), 861-875.
