Investigation of cytokine levels in calves with sepsis

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Published: Jul 10, 2022
DOI: https://doi.org/10.12681/jhvms.26655
Keywords:
Calf sepsis cytokine
H Guzelbektes
Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey. 2Department of Internal Medicine, Faculty of Veterinary Medicine, Kyrgyz Turkish Manas University, Bishkek, Kyrgyzstan.
I Sen
Department of Internal Medicine, Faculty of Veterinary Medicine, Kyrgyz Turkish Manas University, Bishkek, Kyrgyzstan.
U Aydogdu
Department of Internal Medicine, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey. 4Department of Internal Medicine, Faculty of Ceyhan Veterinary Medicine, Cukurova University, Adana, Turkey
C Er
Department of Internal Medicine, Faculty of Ceyhan Veterinary Medicine, Cukurova University, Adana, Turkey.
Alparslan Coşkun
Sivas Cumhuriyet University,
https://orcid.org/0000-0002-2242-9647
Abstract

This study aimed to detect changes in clinical parameters, biochemical and serum cytokine levels in calves with sepsis. In the study, 34 calves diagnosed with sepsis according to clinical and laboratory results and 7 calves deemed to be healthy according to clinical and laboratory results were used. The blood sample was taken from vena jugularis for determination of hematological, biochemical, and cytokine levels. Significant changes were observed in the clinical parameters of calves with sepsis. Serum concentrations of IL-1β, IL-6, IL-10, TNF-α, and INF-γ were significantly increased (p < 0.05) in calves with sepsis compared to the control group. Also, a significant increase (p < 0.05) in serum BUN, creatinine, CK, and CK-MB levels and a significant decrease in TP level (p < 0.05) were determined in calves with sepsis compared to the control group.

In conclusion, it was concluded that varying degrees of tissue/organ damage developed in calves with sepsis and cytokines played an important role in the development of inflammatory damage during sepsis.

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References
Albayrak H, Kabu M (2016) Determining serum haptoglobin and cytokine concentrations in diarrheic calves. F Ü Sağ Bil Vet Derg 30: 113-117.
Aldridge BM, Garry FB, Adams R (1993) Neonatal septicemia in calves: 25 cases (1985-1990). J Am Vet Med Assoc 203: 1324-1329.
Aydogdu U, Coskun A, Yildiz R, Guzelbektes H, Sen I (2018) Clinical importance of lipid profile in neonatal calves with sepsis. J Hellenic Vet Med Soc 69: 1189-1194.
Aydogdu U, Guzelbektes H (2018) Efect of colostrum composition on passive calf immunity in primiparous and multiparous dairy cows. Vet Med 63: 1-11.
Aydogdu U, Yildiz R, Guzelbektes H, Coskun A, Sen I (2016) Cardiac biomarkers in premature calves with respiratory distress syndrome. Acta Vet Hung 64: 38-46.
Aydoğdu U, Yıldız R, Güzelbekteş H, Coşkun A, Şen İ (2019) Yenidoğan ishalli buzağılarda mortalite indikatörü olarak kan laktat, glikoz, total protein ve gama glutamil transferaz seviyeleri. F Ü Sağ Bil Vet Derg 33: 201-206.
Aygun O, Yildiz R (2018) Evaluation of thrombomodulin and pentraxin-3 as diagnostic biomarkers in calves with sepsis. Vet Med 63: 313-320.
Basoglu A, Baspinar N, Tenori L, Hu X, Yildiz R (2014) NMR based metabolomics evaluation in neonatal calves with acute diarrhea and suspected sepsis: A new approach for biomarker/s. Metabolomics 4:134.
Basoglu A, Sen I, Meoni G, Tenori L, Naseri A (2018) NMR-Based plasma metabolomics at set intervals in newborn dairy calves with severe sepsis. Mediators Inflamm 2018:8016510.
Basoglu A, Sen I, Sevinc M, Simsek A (2004) Serum concentrations of tumor necrosis factor-a in neonatal calves with presumed septicemia. J Vet Intern Med 18:238-241.
Baykara N, Akalın H, Arslantaş MK, Hancı V, Çağlayan Ç, Kahveci F, Demirağ K, Baydemir C, Ünal N, Sepsis Study Group (2018) Epidemiology of sepsis in intensive care units in Turkey: a multicenter, point-prevalence study. Crit Care 22:93.
Blackwell TS, Christman JW (1996) Sepsis and cytokines: a current status. Br J Anaesth 77:110-117.
Bodur H, Şencan M, Dökmetaş İ, Elaldı N (2006) Sepsis Ve septik şoklu hastalarda plazma fibrinojen, D-dimer, protein C, protein S ve antitrombin III düzeyleri. C Ü Tıp Fakültesi Dergisi 28: 27-32.
Börekci G, Karakas Celik S, Kandemir O, Aras N, Yalin S (2014) Investigation of IL-1 beta, IL-1 receptor antagonist and IL-8 gene polymorphisms in patients with chronic hepatitis B and C. Mikrobiyol Bul 48:271-282.
Cavaillon JM, Conquy MA (2002) Involvement of pro-and anti-inflammatroy cytokines in sepsis, In: The Sepsis Text. Eds: Vincent JL, Carlet J, Opal SM. Kluwer Academic Publishers, New York: pp 159-198.
Constable PD, Kenneth W, Hinchcliff KW, Done SH, Grünberg W (2017) Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 11th ed, Saunders, Missouri.
Coskun A, Aydogdu U, Guzelbektes H, Sen I (2020) The levels of trace elements and macrominerals in calves with sepsis. Kafkas Univ Vet Fak Derg 26: 351-355.
Drosatos K, Lymperopoulos A, Kennel PJ, Pollak N, Schulze PC, Goldberg IJ (2015) Pathophysiology of sepsis-related cardiac dysfunction: driven by inflammation, energy mismanagement, or both? Curr Heart Fail Rep 12:130-40.
El-Ashker M, El-Sebaei M, Salama M (2012) Evaluation of the inflammatory reaction in calves with acute ruminal drinking. J Vet Sci Technol 3:116.
El-Deeb WM, Tharwat M (2015) Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis. Comp Clin Pathol 24:581-588.
Galvao KN, Felippe MJB, Brittin SB, Sper R, Fraga M, Galvão JS, Caixeta L, Guard CL, Ricci A, Gilbert RO (2012) Evaluation of cytokine expression by blood monocytes of lactating Holstein cows with or without postpartum uterine disease. Theriogenology 77:356-372.
Gelderblom H, Schmidt J, Londoño D, Bai Y, Quandt J, Hornung R, Marques A, Martin R, Cadavid D (2007) Role of interleukin 10 during persistent infection with the relapsing fever Spirochete Borrelia turicatae. Am J Pathol 170:251-62.
Karapinar T, Dabak DO, Kuloglu T, Bulut H (2010) High cardiac troponin I plasma concentration in a calf with myocarditis. Can Vet J 51: 397-399.
Kirbas A, Degirmencay S, Kilinc AA, Eroglu MS (2021) Evaluation of serum cardiac troponin-I concentration and cardiac enzyme activities in neonatal calves with sepsis. Isr J Vet Med 76: 3-10.
Miert AS (1995) Pro-inflammatory cytokines in a ruminant model: pathophysiological, pharmacological, and therapeutic aspects. Vet Q 17:41-50.
Naseri A, Sen I, Turgut K, Guzelbektes H, Constable PD (2019) Echocardiographic assessment of left ventricular systolic function in neonatal calves with naturally occurring sepsis or septic shock due to diarrhea. Res Vet Sci 126: 103-112.
Niewold TA, Tousaint MJM, Gruys E (2003) Monitoring health by acute phase proteins. In: Animal Welfare and Acute Phase Proteins. Proceedings Book of Fourth European Colloquium on Acute Phase Proteins, Segovia, Spain: pp 57-76.
Novelli G, Ferretti G, Poli L, Pretagostini R, Ruberto F, Perrella SM, Levi Sandri GB, Morabito V, Berloco PB (2010) Clinical results of treatment of postsurgical endotoxin-mediated sepsis with polymyxin-B direct hemoperfusion. Transplant Proc 42:1021-1024.
Okajima K (2001) Regulation of inflammatory responses by natural anticoagulants. Immunol Rev 184:258-274.
Peek SF, Apple FS, Murakami MA, Crump PM, Semrad SD (2008). Cardiac isoenzymes in healthy Holstein calves and calves with experimentally induced endotoxemia. Can J Vet Res 72:356-361.
Pinsky MR, Vincent JL, Deviere J, Alegre M, Kahn RJ, Dupont E (1993) Serum cytokine levels in human septic shock: Relation to multiple-system organ dysfunction and mortality. Chest 103:565-75.
Qiu G, Wang C, Smith R, Harrison K, Yin K (2001) Role of IFN-gamma in bacterial containment in a model of intra-abdominal sepsis. Shock 16:425-429.
Riche FC, Cholley BP, Panis YH, Laisne MJ, Briard CG, Graulet AM, Gueris JL, Valleur PD (2000) Inflammatory cytokine response in patients with septic shock secondary to generalized peritonitis. Crit Care Med 28:433-437.
Schulte W, Bernhagen J, Bucala R (2013) Cytokines in sepsis: Potent immunoregulators and potential therapeutic targets-an updated view. Hindawi Publishing Corporation Mediators of Inflammation,165974:16.
Sharma S, Yang B, Xi X, Grotta JC, Aronowski J, Savitz SI (2011) IL-10 directly protects cortical neurons by activating PI-3 kinase and STAT-3 pathways. Brain Res 1373:189-194.
Tsiotou AG, Sakorafas GH, Anagnostopoulos G, Bramis J (2005) Septic shock; current pathogenetic concepts from a clinical perspective. Med Sci Monit 11:RA76-85.
van Gucht S, Labarque G, Reeth KV (2004) The combination of PRRS virus and bacterial endotoxin as a model for multifactorial respiratory disease in pigs. Vet Immunol Immunopathol 102:165-178.
Worthley LI (2000) Shock: a review of pathophysiology and management. Part II. Crit Care Resusc 2:66-84.
Yıldız R, Beslek M, Beydilli Y, Özçelik M, Biçici Ö (2000) Evaluation of platelet activating factor in neonatal calves with sepsis. Vet Hekim Der Derg 89: 66-73.
Yilma AN, Singh SR, Fairley SJ, Taha MA, Dennis VA (2012) The anti-inflammatory cytokine, interleukin-10, inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV-inactivated Chlamydia trachomatis. Mediators Inflamm 2012:520174.
Zhang JM, An J (2007) Cytokines, inflammation and pain. Int Anesthesiol Clin 45: 27-37.