Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep
Résumé
The aim of this study was to examine the polymorphic structures of the Myostatin (MSTN) gene locus in the native Morkaraman sheep, and to determine the distribution of genotype and allele frequencies. MSTN/HaeIII gene polymorphisms were identified in DNA isolated from blood samples from 262 Morkaraman sheep used in the study, using the PCR-RFLP method. According to the Hardy-Weinberg genetic balance test, it was observed that the distribution of genotype frequencies was not in balance (P<0.01) in the population studied. The MM, Mm and mm genotype frequencies of the MSTN gene in the population were determined as 11.1%, 62.6% and 26.3%, respectively, the frequencies of the M and m allele was 0.42 and 0.58 respectively. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values.While the FIS value was found as -0.312, the FIT value was determined as -0.303. The genotype and allele frequencies determined in terms of MSTN gene polymorphism were sufficient to reveal the genotype diversity of the breed.
Article Details
- Comment citer
-
Saygili, E., & Ozdemir, M. (2023). Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep. Journal of the Hellenic Veterinary Medical Society, 74(1), 5449–5456. https://doi.org/10.12681/jhvms.29799 (Original work published 12 avril 2023)
- Numéro
- Vol. 74 No 1 (2023)
- Rubrique
- Research Articles
Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
