Isolation, identification and genetic variation analysis of pseudorabies virus mutant strain from Chinese pig farm


Publiée : Jul 10, 2024
Mis à jour : 2024-07-10
Versions :
2024-07-10 (2)
B Zhou
X Wang
C Xin
J Lin
S Dong
Y Wen
R Zhang
H Shan
Hongliang Zhang
Résumé

ABSTRACT: Pseudorabies (PR) is an important infectious disease affecting pig farms worldwide. In this study, we reported a pseudorabies virus (PRV) isolated from a Bartha-K61-vaccinated pig farm in Linyi, Shandong Province, China. Evidence from virus isolation, electronic microscope observation,laboratory animal infection, and histopathologic examination confirmed that the etiological agent of the disease is PRV SD-2017. Sequence alignment of the gE gene indicated that it belongs to a new mutated PRV strain. The gB sequence alignment showed that compared with the Bartha and Kaplan genotype 1 strains, SD-2017 strain had a deletion of three amino acids at positions 75-78 (SPG) and an insertion of one amino acid at position 94 (G). The gC gene had 7 amino acids (A, A, A, S, T, P and A) inserted at positions 69-75 in comparison with the Bartha-K61 vaccine strain. The median lethal dose (LD50) of PRV SD-2017 strain on rabbits was 3.16×102.0TCID50/ml by Karber method. Histopathologic examinations showed that multiple lesion sites were observed in brains, lungs, livers and kidneys. PRV SD-2017 is different from other reports and should be paid more attention to avoid economic losses.


Keywords: Pseudorabies virus; Sequence alignment; Amino acid mutations;Mutant strain

Article Details
  • Rubrique
  • Research Articles
Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Biographie de l'auteur
X Wang

College of Veterinary Medicine, Shandong Agricultural University, Taian, China

Références
Ai JW, Weng SS, Cheng Q, Cui P, Li YJ, Wu HL, Zhu YM, Xu B, Zhang
WH (2018): Human endophthalmitis caused by pseudorabies virus
infection, China, 2017. Emerg. Infect. Dis. 24, 1087-1090.
An TQ, Peng JM, Tian ZJ, Zhao HY, Li N, Liu YM, Chen JZ, Leng CL,
Sun Y, Chang D, Tong GZ (2013): Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012. Emerg. Infect. Dis. 19, 1749-
Fan J, Zeng X, Zhang G, Wu Q, Niu J, Sun B, Xie Q, Ma J (2016): Molecular characterization and phylogenetic analysis of pseudorabies virus
variants isolated from Guangdong province of southern China during
-2014. J. Vet. Sci. 17 (3), 369.
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M,
Zhou X (2008): Geminivirus strain demarcation and nomenclature.
Arch. Virol. 153 (4), 783-821.
Freuling CM, Muller TF, Mettenleiter TC (2017) Vaccines against pseudorabies virus (PrV). Vet. Microbiol. 206, 3-9.
Gu Z, Hou C, Sun H, Yang W, Dong J, Bai J, Jiang P (2015): Emergence
of highly virulent pseudorabies virus in southern China. J. Vet. Res.
(3), 221-228.
Karger A, Schmidt J, Mettenleiter TC (1998): Infectivity of a pseudorabies virus mutant lacking attachment glycoproteins C and D. J. Virol.
(9), 7341-7348.
Mettenleiter TC (2000): Aujeszky’s disease (pseudorabies) virus: the virus
and molecular pathogenesis--state of the art, June 1999. Vet. Res. 31
(1), 99-115.
Mettenleiter TC (2003): Pathogenesis of neurotropic herpesviruses: role
of viral glycoproteins in neuroinvasion and transneuronal spread. Virus Res. 92 (2), 197-206.
Mettenleiter TC, Klupp BG, Weiland F, Visser N (1994): Characterization of a quadruple glycoprotein-deleted pseudorabies virus mutant
for use as a biologically safe live virus vaccine. J. Gen. Virol. 75 ( Pt
, 1723-1733.
Müller T, Hahn EC, Tottewitz F, Kramer M, Klupp BG, Mettenleiter TC,
Freuling C (2011): Pseudorabies virus in wild swine: a global perspective. Arch. Virol. 156 (10), 1691-1705.
Okazaki K (2007): Proteolytic cleavage of glycoprotein B is dispensable
for in vitro replication, but required for syncytium formation of pseudorabies virus. J. Gen. Virol. 88 (Pt 7), 1859-1865.
Pensaert MBKP (1989): Pseudorabies virus (Aujeszky’s disease), In: Pensaert, M. (Ed.) Virus Infections of Porcines. Elsevier Science Publishers: New York.
Pomeranz LE, Reynolds AE, Hengartner CJ (2005): Molecular biology
of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69 (3), 462-500.
Sun Y, Liang W, Liu Q, Zhao T, Zhu H, Hua L, Peng Z, Tang X, Stratton
CW, Zhou D, Tian Y, Chen H, Wu B (2018) Epidemiological and
genetic characteristics of swine pseudorabies virus in mainland China
between 2012 and 2017. PeerJ (San Francisco, CA) 6:e5785.
Tang Y, Liu J, Wang T, An T, Sun M, Wang S, Fang Q, Hou L, Tian Z,
Cai X (2016): Live attenuated pseudorabies virus developed using the
CRISPR/Cas9 system. Virus Res. 225, 33-39.
Wang CH, Yuan J, Qin HY, Luo Y, Cong X, Li Y, Chen J, Li S, Sun Y,
Qiu HJ (2014): A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the
PRV variant emerging in Bartha-K61-vaccinated swine population in
China. Vaccine 32 (27), 3379-3385.
Wang Y, Qiao S, Li X, Xie W, Guo J, Li Q, Liu X, Hou J, Xu Y, Wang L,
Guo C, Zhang G (2015): Molecular epidemiology of outbreak-associated pseudorabies virus (PRV) strains in central China. Virus Genes
(3), 401-409.
Wong G, Lu J, Zhang W, Gao GF (2019) Pseudorabies virus: a neglected
zoonotic pathogen in humans? Emerging Microbes & Infections 8 (1),
-154.
Wozniakowski G, Samorek-Salamonowicz E (2015): Animal herpesviruses and their zoonotic potential for cross-species infection. Ann Agric
Environ Med 22 (2), 191-194.
Wu X, Chen Q, Chen R, Che Y, Wang L, Wang C, Yan S, Liu Y, Xiu J,
Zhou L (2017): Pathogenicity and Whole Genome Sequence Analysis
of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China. Can J Infect Dis Med Microbiol. 2017:1-8.
Yang H, Han H, Wang H, Cui Y, Liu H, Ding S (2019): A Case of Human
Viral Encephalitis Caused by Pseudorabies Virus Infection in China.
Front Neurol. 10.
Yang X, Guan H, Li C, Li Y, Wang S, Zhao X, Zhao Y, Liu Y (2019):
Characteristics of human encephalitis caused by pseudorabies virus:
A case series study. Int. J. Infect. Dis. 87, 92-99.
Ye C, Guo J, Gao J, Wang T, Zhao K, Chang X, Wang Q, Peng J, Tian Z,
Cai X, Tong G, An T (2016): Genomic analyses reveal that partial
sequence of an earlier pseudorabies virus in China is originated from
a Bartha-vaccine-like strain. Virology 491, 56-63.
Ye C, Zhang QZ, Tian ZJ, Zheng H, Zhao K, Liu F, Guo JC, Tong W, Jiang
CG, Wang SJ, Shi M, Chang XB, Jiang YF, Peng JM, Zhou YJ, Tang
YD, Sun MX, Cai XH, An TQ, Tong GZ (2015): Genomic characterization of emergent pseudorabies virus in China reveals marked
sequence divergence: Evidence for the existence of two major genotypes. Virology 483, 32-43.
Yu T, Chen F, Ku X, Fan J, Zhu Y, Ma H, Li S, Wu B, He Q (2016):
Growth characteristics and complete genomic sequence analysis of a
novel pseudorabies virus in China. Virus Genes 52, 474-83.
Yu X, Zhou Z, Hu D, Zhang Q, Han T, Li X, Gu X, Yuan L, Zhang S,
Wang B, Qu P, Liu J, Zhai X, Tian K (2014): Pathogenic Pseudorabies
Virus, China, 2012. Emerg. Infect. Dis. 20, 102-104.
Zhai X, Zhao W, Li K, Zhang C, Wang C, Su S, Zhou J, Lei J, Xing G,
Sun H, Shi Z, Gu J (2019): Genome Characteristics and Evolution of
Pseudorabies Virus Strains in Eastern China from 2017 to 2019. Virol
Sin. 34, 601-609.
Zhou M, Wu X, Jiang D, Sui C, Chen L, Cong X, Xin X, Wang G, Li Y,
Tian F, Chen Z, Zhang H, Qi J, Wang Z, Wu J, Shan H, Du Y (2019):
Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014. Infect. Genet. Evol. 68, 161-171.
Zhang L, Ruan K, Sang G, Xu Z, Tong W, Yu H, Shan T, Gao F, Li L,
Kong N (2021): Tk-deleted Pseudorabies Virus Retains High Pathogenicity in Rats. J Vet Res, 65:401-405