Effects of exogenous progesterone on the ovine serum progesterone profile during the non-breeding season

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Publiée : Οκτ 22, 2024
DOI : https://doi.org/10.12681/jhvms.34759
F Barati
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
P Hosseini
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
M Azari
Department of animal affairs, Jihad-agriculture organization, Chaharmahal, and Bakhtiari, Shahrekord, Iran.
Résumé

Exogenous progesterone (PRG) sources are used to induce ovine estrus during the non-breeding season. Intramuscular injection (IM) of PRG is an uncommon route for this purpose. This study investigated serum progesterone (P4) levels after IM administration of 50 mg of two different PRG products. Ten ewes were selected and received two consecutive PGF2α injections at 24-h intervals. Serum samples were collected (Time 0), followed by the injection of single doses of Fertigest® or Vetagesterone®. Serum P4 levels were assayed at times (T) 0, 30 min, 3,6,12,24,48,72,96, and 120 h after injection. A large variation in the serum P4 levels at T0 was detected. The serum P4 level at T0 was 4.3 ng/mL, which is considerable for anestrus ewes, following PGF2α administration. The mean time to reach maximum serum P4 concentration was 5.8 h, with a mean concentration of 22.5 ng/mL. Serum P4 levels returned to basal values (T0) after 48 h. An increased P4 production rate (PR) was detected following PRG administration, which continued for 24 h. A single injection of PRG reduced the variety of serum P4 levels from 24 h onward. The two different PRG generated different serum P4 concentrations. This study indicates an unusual change in serum P4 levels in anestrus ewes following PGF2α administration. 48 h after 50 mg PRG injection, the plasma levels of P4 reached basal levels in anestrus ewes.

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Bibliographies de l'auteur
P Hosseini, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

He is a DVSc candidate for theriogenology at SKU, Shahrekord, Iran

M Azari, Department of animal affairs, Jihad-agriculture organization, Chaharmahal, and Bakhtiari, Shahrekord, Iran.

Principle conductor of Shooli station for Bakhtiari sheep conservation.

Références
Awel H, Eshetu L, Tadesse G, Birhanu A, Khar SK (2009) Estrus synchronization in sheep with synthetic progestagens. Trop Anim Health
Product 41:1521-1524. https://doi.org/ 10.1007/s11250-009-9342-7
Ayaseh M, Mirzaei A, Boostani A, Mehrvarz M (2021) The effect of prostaglandin and gonadotrophins (GnRH and hCG) injection combined
with the ram effect on progesterone concentrations and reproductive
performance of Karakul ewes during the non‐breeding season. Vet
Med Sci 7:148-155. https://doi.org/10.1002/vms3.353.
Barker-Gibb M, Clarke IJ (2000) Effect of season on neuropeptide Y and
galanin within the hypothalamus of the ewe in relation to plasma luteinizing hormone concentrations and the breeding season: an immunohistochemical analysis. J Neuroendocrinol 12:618-626. https://doi.
org/10.1046/j.1365-2826.2000.00514.x.
Bedford CA, Harrison F, Heap R (1972) The metabolic clearance rate and
production rate of progesterone and the conversion of progesterone to
α-hydroxypregn-4-en-3-one in the sheep. J Endocrinol 55:105-118.
https://doi.org/10.1677/joe.0.0550105.
Berean D, Ergene O, Blaga-Petrean A, Bogdan I, Ciupe S, Cenariu M,
Pall E, Bogdan LM (2021) Comparative data about estrus induction
and pregnancy rate on lacaune ewes in non-breeding season after
melatonin implants and intravaginal progestagen. Indian J Anim Res
:517-521. https://doi.org/ 10.18805/IJAR.B-1282.
Chamley WA, Jonas HA, Parr R (1976) Content of LH, FSH, and growth
hormone in the pituitaries of pregnant and anestrous sheep. Endocrinol 98:1535-1538. https://doi.org/10.1210/endo-98-6-1535.
Dehkordi RS, Mirzaei A, Boostani A (2022) Reproductive efficiency of
treated Karakul ewes with short-term progesterone and hCG injections during the non-breeding and breeding seasons. Anim Reprod
Sci 239. https://doi.org/ 10.1016/j.anireprosci.2022.106969
Goodman RL, Maltby MJ, Millar RP, Hileman SM, Nestor CC, Whited
B, Tseng AS, Coolen LM, Lehman MN (2012) Evidence that dopamine acts via kisspeptin to hold GnRH pulse frequency in check in
anestrous ewes. Endocrinol 153:5918-5927. https://doi.org/10.1210/
en.2012-1611.
Hashemi M, Safdarian M, Kafi M (2006) Estrous response to synchronization of estrus using different progesterone treatments outside the
natural breeding season in ewes. Small Rum Res 65:279-283. https://
doi.org/ 10.1016/j.smallrumres.2005.07.051.
Manca ME, Manunta ML, Spezzigu A, Torres-Rovira L, Gonzalez-Bulnes
A, Pasciu V, Piu P, Leoni GG, Succu S, Chesneau D, Naitana S, Berlinguer F (2014) Melatonin deprival modifies follicular and corpus
luteal growth dynamics in a sheep model. Reproduction 147:885-895.
https://doi.org/ 10.1530/REP-13-0405.
Mansoor AR, Saleh ID, Dakheel HA (2020) Effect of addition of melatonin to the intravaginal sponges on oxidative status and reproductive performance in Iraqi awassi ewes. Ann Trop Med Public Health.
:120-127. https://doi.org/ 10.36295/ASRO.2020.23218.
Niasari-Naslaji A, Seidi Samani H, Shojaei A, Ganjkhanlou M, Baninajar
M (2022) Induction of estrus and fertility of Shal ewes using progesterone injections or intravaginal sponge followed by laparoscopic
AI during non-breeding season. Iran Vet J. https://doi.org/ 10.22055/
ivj.2021.300556.2389.
Thiéry J, Robel P, Canepa S, Delaleu B, Gayrard V, Picard‐Hagen N, Malpaux B (2003) Passage of progesterone into the brain changes with
photoperiod in the ewe. Eur J Neurosci 18:895-901
Thiery JC, Lomet D, Schumacher M, Liere P, Tricoire H, Locatelli A,
Delagrange P, Malpaux B (2006) Concentrations of estradiol in
ewe cerebrospinal fluid are modulated by photoperiod through pineal‐dependent mechanisms. J Pineal Res. 41:306-312. https://doi.
org/10.1046/j.1460-9568.2003.02796.x.
Yu XJ, Wang J, Bai YY (2018) Estrous synchronization in ewes: The use
of progestogens and prostaglandins. Acta Agric Scand A: Anim Sci
:219-230. https://doi.org/ 10.1080/09064702.2019.1674373