Comparison of methods for measurement of somatic cell count in goat milk somatic cells

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Publiée : Oct 24, 2024
DOI : https://doi.org/10.12681/jhvms.35582
NM Zazharska
Dnipro State Agrarian and Economic University, Faculty of Veterinary Medicine, Yefremov Str., 25, Dnipro, 49000, Ukraine
Résumé

The aim of the study was to compare the efficiency of determining the number of somatic cell count in goat milk by different methods. Somatic cells in individual samples of milk from 28 goats were studied using analyzers "Somatos", "SomaCount Flow Cytometer" and by the counting method in milk films stained according to Romanovsky – Giemsa, May-Grünwald and with pyronin Y. For the counting of somatic cells in goat milk by Prescott and Breed method, it is recommended to stain milk films by the May-Grünwald method, because the cytoplasm and nuclei of somatic cells are clearly stained, and the cost of dyes is much less than the pyroninY method. When counting cells in milk films stained by any method, no samples with the SCC up to 100 ´ 103 cells/ml were found. Direct counting of somatic cells in milk films stained by any method reveals a greater number of cells than with the help of devices. It was found that the fat content in goat milk with SCC >3000 ´ 103 cells/ml is significantly higher (P < 0.05) compared to milk with SCC < 1000 ´ 103 cells/ml. The quality of goat's milk smears for counting of somatic cells quantity stained by the May-Grünwald method corresponds to the recommended method with pyronin Y, and the cost of dyes is 28.4 times lower. The distribution of somatic cell ranges is similar between different methods of staining smears, which once again proves the accuracy of the method of direct cell counting in milk films, although it is more labor-intensive than hardware methods.

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Références
Bagnicka E, Łukaszewicz M, Ådnøy T (2016) Genetic parameters of somatic
cell score and lactose content in goat’s milk. J Anim Feed Sci
(3):210-215. https://doi.org/10.22358/jafs/65552/2016
Borovuk I, Zazharska N (2022) Evaluation of broiler meat in experimental
listeriosis. J Adv Vet Anim Res 9(1):155-165. https://doi.org/10.5455/
javar.2022.i580
Boutinaud M, Guinard-Flament J, HélèneJammes (2004) The number and
activity of mammary epithelial cells, determining factors for milk
production. Reprod Nutr Dev 44(5):499-508. https://doi.org/10.1051/
rnd:2004054
de Vasconcelos ÂM, Martins TP, de Souza V, Bonfim JM, Pompeu RCFF,
Façanha DAE et al (2023) Effect of a 60-day organic selenium-supplemented
diet on the decrease of somatic cell counts in goat milk.
Trop Anim Health Prod 55(2). https://doi.org/10.1007/s11250-023-
-2
Erduran H (2023) Effect of parturition time and photoperiod on milk production,
quality, and somatic cell count traits of pure and crossbred
goats in a different production system. Trop Anim Health Prod 55(3).
https://doi.org/10.1007/s11250-023-03560-0
Fotina T, Fotina H, Ladyka V, Ladyka L, Zazharska N (2018) Monitoring
research of somatic cells count in goat milk in the eastern region
of Ukraine. J Hellenic Vet Med Soc 69(3):1101-1108. https://doi.
org/10.12681/jhvms.18882
Gautam PB, Sharma R, Atbhaiya Y, Gandhi K, Mann B (2023) Activities
of indigenous proteases in cow, buffalo and goat milk of Indian subcontinent
and their correlation with somatic cell count. Int Dairy J
:105567. https://doi.org/10.1016/j.idairyj.2022.105567
Gómez-Gascón L, Galán-Relaño A, Cardoso-Toset F, Barrero-Domínguez
B, Astorga RJ, Luque I, et al (2022) Lactate dehydrogenase: Detecting
high bacterial and somatic cells counts in goats from whole milk
samples. Small Rumin Res 208:106632. https://doi.org/10.1016/j.
smallrumres.2022.106632
Gotsulya АS, Zazharskyі VV, Davidenko PO, Zazharska NM, Kulishenko
OM, Panasenko OI, et al (2020) Features of experimental modeling
of tuberculosis in guinea pig with the participation of N’-(2-(5-((thephylline-
’-yl)methyl)-4-R-1,2,4-triazole-3-ylthio)acethyl)isonicotinohydrazide.
Ukr J Ecol 10(4):191-194. doi: 10.15421/2020_187
Hisira V, Zigo F, Kadaši M, Klein R, Farkašová Z, Vargová M, Mudroň
P (2023) Comparative analysis of methods for somatic cell counting
in cow’s milk and relationship between somatic cell count and
occurrence of intramammary bacteria. Vet. Sci. 10:468. https://doi.
org/10.3390/vetsci10070468
Kavitha G, Isloor S, Rathnamma D, Rajeshwari Y, Nagaraja C, Sadish S
(2011) Induction of mastitis in rabbit mammary glands with bovine
mastitis bacterial strains. Int J Pharma Bio Sci 2(3):266-276.
Leitner G, Silanikove N, Merin U (2008) Estimate of milk and curd yield
loss of sheep and goats with intrammamary infection and its relation
to somatic cell count. Small Rumin Res 74(1-3):221-225. https://doi.
org/10.1016/j.smallrumres.2007.02.009
Lianou DT, Michael CK, Gougoulis DA, Cripps PJ, Vasileiou NGC, Solomakos
N, et al (2022) High milk somatic cell counts and increased
teladorsagia burdens overshadow non-infection-related factors as predictors
of fat and protein content of bulk-tank raw milk in sheep and
goat farms. Foods 11(3):443. https://doi.org/10.3390/foods11030443
Looper M (2013) Reducing Somatic Cell Count in Dairy Cattle. Agr Nat
Resour https://www.uaex.edu/publications/PDF/FSA-4002.pdf
Pajor F, Sramek А, Tоth G, Pоti P (2013) Effect of somatic cell count on
some chemical, physical and bacterial properties of milk in a Hungarian
Alpine goat farm. Goat Milk Quality Regional IGA Conference,
Norway. Abstracts 2013:38.
Raynal-Ljutovac K, Gaborit P, Lauret A (2005) The relationship between
quality criteria of goat milk, its technological properties and the quality
of the final products. Small Rumin Res 60(1-2):167-177. https://
doi.org/10.1016/j.smallrumres.2005.06.010
Raynal-Ljutovac K, Pirisi A, de Crémoux R, Gonzalo C (2007) Somatic
cells of goat and sheep milk: Analytical, sanitary, productive and
technological aspects. Small Rumin Res 68(1-2):126-144. https://doi.
org/10.1016/j.smallrumres.2006.09.012
Rozos G, Skoufos I, Fotou K, Alexopoulos A, Tsinas A, Bezirtzoglou E et
al (2022) Safety issues regarding the detection of antibiotics residues,
microbial indicators and somatic cell counts in ewes’ and goats’ milk
reared in two different farming systems. Appl Sci 12(3):1009. https://
doi.org/10.3390/app12031009
Silanikove N, Merin U, Leitner G (2014) On effects of subclinical mastitis
and stage of lactation on milk quality in goats. Small Rumin Res
(1-3):76-82. https://doi.org/10.1016/j.smallrumres.2014.07.018
Silanikove N, Leitner G, Merin U, Prosser C (2010) Recent advances
in exploiting goat’s milk: Quality, safety and production aspects.
Small Rumin Res 89:110-124. https://doi.org/10.1016/j.smallrumres.
12.033
Sklyarov P, Fedorenko S, Naumenko S (2020) Oxidant/Antioxidant Balance
in Cows and Sheep in Antenatal Pathology. Ukr J Ecol 10(5):26-
https://doi.org/10.15421/2020_201
Šlyžius E, Anskienė L, Palubinskas G, Juozaitienė V, Šlyžienė B, Juodžentytė
R et al (2023) Associations between somatic cell count and milk
fatty acid and amino acid profile in alpine and saanen goat breeds.
Animals 13(6):965. https://doi.org/10.3390/ani13060965
Smistad M, Vatne MK, Sølverød L, Dean KR (2022) Sensitivity and specificity
of bacterial culture, qPCR, and somatic cell count for detection
of goats with Staphylococcus aureus intramammary infection using
Bayesian latent class models. Prev Vet Med 209:105793. https://doi.
org/10.1016/j.prevetmed.2022.105793
Soquila SS (2023). Direct and indirect measurement of somatic cell count
in detecting subclinical mastitis in goats. TSRJ 18(6):1085-1090.
https://doi.org/10.17605/OSF.IO/9Z82Q
Stuhr T, Aulrich K, Barth K, Knappstein K, Larsen T (2013) Influence
of udder infection status on milk enzyme activities and somatic cell
count throughout early lactation in goats. Small Rumin Res 111(1-
:139-146. https://doi.org/10.1016/j.smallrumres.2012.09.004
Sun X, Zhao R, Wang N, Zhang J, Xiao B, Huang F et al (2023) Milk somatic
cell count: From conventional microscope method to new biosensor-
based method. Trends Food Sci Technol 135:102-114. https://
doi.org/10.1016/j.tifs.2023.03.020
Sоlverоd L (2013) Udder health in Norwegian goat dairy herds. Goat Milk
Quality Regional IGA Conference, Norway. Abstracts 2013:49.
Tvarožková K, Tančin V, Uhrinčať M, Oravcová M, Hleba L, Gancárová
B et al (2023) Pathogens in milk of goats and their relationship with
somatic cell count. J Dairy Res. 2023:1-5. https://doi.org/10.1017/
s0022029923000237
Vasil’ M, Farkasova Z, Elecko J, Illek J, Zigo F (2017) Comparison of
biofilm formation by Staphylococcus aureus and Staphylococcus
epidermidis strains isolated from sheep milk using three diagnostic
methods. Pol J Vet Sci 20(4):795-801. doi: 10.1515/pjvs-2017-0100.
PMID: 29611650.
Vasylieva N (2021) Food security in times of covid-19: price aspects in
Ukraine and neighboring EU countries. MNJE 17(3):21-30. https://
doi.org/10.14254/1800-5845/2021.17-3.2
Yangilar, F., 2013. As a potentially functional food: Goats’ milk and products.
J. Food Nutr. Res., 1(4): 68-81.
Zazharska N, Fotina T, Yatsenko I, Tarasenko L, Biben I, Zazharskyi V et
al (2021) Comparative analysis of the criteria for goat milk assessment
in Ukraine and France. Ukr J Ecol 11(2):144-148. http://doi.
org/10.15421/2021_91
Zazharskyi V, Bigdan O, Parchenko V, Parchenko M, Fotina T, Davydenko
P et al (2021) Antimicrobial Activity of Some Furans Containing
,2,4- Triazoles. Arch Pharm Pract 12(2):60-65. https://doi.
org/10.51847/rbjb3waubb
Zazharskyi V, Parchenko M, Fotina T, Davydenko P, Kulishenko O,
Zazharskaya N et al (2019) Synthesis, structure, physicochemical
properties and antibacterial activity of 1,2,4-triazoles-3-thiols and
furan derivatives. Vopr Khimii i Khimicheskoi Tekhnologii 6:74-82.
https://doi.org/10.32434/0321-4095-2019-127-6-74-82
Zazharskyi V, Parchenko M, Parchenko V, Davydenko P, Kulishenko
O, Zazharska N (2020) Physicochemical properties of new S-derivatives
of 5-(5-bromofuran-2-yl)-4-methyl-1,2,4-triazol-3-thiols.
Vopr Khimii i Khimicheskoi Tekhnologii 6:50-58. https://doi.
org/10.32434/0321-4095-2020-133-6-50-58
Zigo F, Elečko J, Farkašová Z, Zigová M, Vasiľ M, Ondrašovičová S,
Lenka K (2019) Preventive methods in reduction of mastitis pathogens
in dairy cows. J Microbiol Biotechnol Food Sci 9(1):121-126.
https://doi.org/10.15414/jmbfs.2019.9.1.121-126
Zigo F, Farkasóvá Z, Eleko J, Lapin M, Chripková M, Czerski A (2014)
Effect of parenteral administration of Selenium and vitamin E on
health status of mammary gland and on selected antioxidant indexes
in blood of dairy cows. Pol J Vet Sci 17(2):217-223. https://doi.
org/10.2478/pjvs-2014-0031
Zigo F, Farkašová Z, Výrostková J, Regecová I, Ondrašovičová S, Vargová
M, Sasáková N, Pecka-Kielb E, Bursová Š, Kiss DS (2022)
Dairy Cows’ Udder Pathogens and Occurrence of Virulence Factors
in Staphylococci. Animals 12(4):470. https://doi.org/10.3390/
ani12040470