Serotypic diversity and sequence variation of the ompA gene among Mannheimia haemofytica isolates from domestic ruminants

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Pubblicato: Dec 15, 2017
DOI: https://doi.org/10.12681/jhvms.15389
C. VOUGIDOU (Χ. ΒΟΥΓΙΔΟΥ)
Institute of Infectious and Parasitic Diseases of Thessaloniki, Ministry of Rural Development and Food
V. SANDALAKIS (Β. ΣΑΝΤΑΛΑΚΗΣ)
laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, WHO Collaborating Center;Regional Laboratory of Public Health (Crete)
A. PSAROULAKI (Α. ΨΑΡΟΥΛΑΚΗ)
laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, WHO Collaborating Cente
E. PETRIDOU (Ε.Ι. ΠΕΤΡΙΔΟΥ)
Laboratory? of Microbiology- and Infectious Diseases, Veterinary- School, Aristotle University of Thessaloniki
W. DONACHIE
'Moredun Research Institute, Pent lands Science Park
L. EKATERINIADOU (Λ.ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ)
Ινστιτούτο Κτηνιατρικών Ερευνών Θεσσαλονίκης, ΕΘΙΑΓΕ
Abstract

Pneumonia caused by Mannheìmia haemolytica is an important disease of ruminants. Because of its economic significance, several methods have been used to study the pathogenicity and epidemiology of M. haemolytica. The objectives of this study were to provide data about the prevalence of the different serotypes of the bacterium and to investigate the genetic diversity of a significant virulence factor, the ompA gene. Two methods, DNA sequencing analysis and DGGli, were used to study the polymorphisms of the ompA gene. Ninety-four isolates from pneumonic lungs were investigated. Capsular scrotyping showed that serotype Λ2 was the predominant serotype among ovine strains and the only serotype found in caprine strains. This is the first reported analysis of the ompA gene of M haemolytica strains isolated from goats. Analysis of the gene revealed five DGGli patterns and nine genotypic groups. The ovine isolates, which belonged to four DC G li patterns, showed a much greater diversity than the caprine strains, which belonged to just two DGGF patterns, Sequence analysis was used to verify the DGGE results and revealed eight genotypic groups among the ovine isolates and three among the caprine ones. Furthermore, the correlation of these results showed a great diversity of the ompA gene among serotype A2 isolates.

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Riferimenti bibliografici
Adlam C (1989) The structure, function and properties of cellular and extracellular components of Pasieurella haemolyiica. In: Pasieurella and pastcurcllosis, Academic Press, London; pp 75- 92.
Alexander TW, Cook SR, Yanke LJ, Booker CW, Morley PS, Read RR, Gow SP, McAllister TA (2008) A multiplex polymerase chain reaction assay for the identification of Mannheimia haemolyiica, Mannheìmìa glucosida and Mannheìmìa nuninaIis. Vet Microbiol 130:165-175.
Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard M (1999) Taxonomic relationships of the [Pasteurella] haemolyiica complex as evaluated by DNA-DNA hybridisations and 16S rRNA sequencing with proposal of Mannheim la haemolyiica gent nov,, comb, nov,, Mannheimia granulomaiis comb, nov., Mannheìmìa glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheìmìa varigena sp, nov, Int. J. Syst Evol Microbiol 49:67-86.
Angen O, Ahrens P, Bisgaard M (2Ö02) Phenolypic and genotypic characterization of Mannheìmìa (Pasteurella) haemolytìca-like strains isolated from diseased animals in Denmark, Vet Microbiol 84:103-114.
Ayalew S, Shrestha B, Montelongo M, Wilson ΛΕ, Confer AW (2011) Immunogcnicity of Mannheìmìa haemolytica recombinant outer membrane proteins SSA-1, OmpA, OmpP2, and OmpD15. Clin Vaccine Immunol 18:2067-74
Bcachey EH (1981) Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Pis 143:325-345.
Beher MG, Schnaitman CA, Pugsley ΛΡ (1980) Major heat-modifiable outer membrane protein in gram-negative bacteria: comparison with the ompA prolein of Escherichia coli. J Bactcriol 143:906-913,
Blackall PJ, Bisgaard M, Stephens CP (2002) Phenolypic characterisation of Australian sheep and cattle isolates of Mannheìmìa haemolyiica, Mannheìmìa granulomaiis and Mannheìmìa varigena, Aust Vet J 80:87-91
Blackall PJ, Bojesen AM, Christensen H, Bisgaard M (2007) Reclassification of [Pasteurella] trehalosi as Bihersielnia trehalosi gen, now, comb, nov, ïnt J Syst Evol Microbiol 57:666-674.
Brogden KA, Lehmkuhl HD, Cullip RC (1998) Pasteurella haemolyiica complicated respiratory infections in sheep and goats, Vet Res 29:233-254
Dabo SM, Confer AW, Quijano-Blas RA (2003) Molecular and immunological characterization of Pasteurella mullocida serotype A:3 OmpA; evidence of its role in Pr mullocida interact ion with extracellular matrix molecules. Mie rob Palhog 35:147-157
Davics RL, Donachic W (1996) ïntra-specific diversity and host specificity within Pasteurella haemolyiica based on νariation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins, Microbiology 142:1895-1907
Davies RL, Arkinsaw S, Selander RK. ( 1997) Evolutionary genetics of Pasteurella haemolyiica isolates recovered from cattle and sheep. Infect Immun 65:3585-3593.
Davies RL, Lee I (2004) Sequence diversity and molecular evolution of the heat-modiflable outer membrane protein gene [ompA) of Mannheìmìa (Pasteurella) haemolyiica, Mannheìmìa glucosida, and Pasteurella irehalosi. J Bacteriol 186:5741-5752.
Fisher SG and Lcrman LS (1983) DNA fragments differing by single base-pair substitutions arc separated in denaturing gradient gels: correspondence with theory. P. Natl. Acad. Sci. USA. 80:579-1583
Frank GH (1989) Pasteurcllosis of cattle, In: Pasteurella and pasteurellosis. Academic Press, London: pp 197-222
Fraser J, Donachic W, Quiric Mt Gilmour Ν J (1983) Rapid indirect hemagglutination test for serotyping Pasteurella haemolyiica. J Clin Microbiol 18:206-207.
Gilmour NJL, Gilmour JS (1989) Pasteurellosis of sheep. In: Pasteurella and pasteurellosis, Academic Press, London: pp 223-262.
Gürtler V, Barric Η13, Mayall BC (2001) Use of denaturing gradient gel electrophoresis to detect mutation in VS2 of the 16S-23S rDNA spacer amplified from Siaphylococcus aureus isolates, Electrophoresis 22:1920-1924.
Highlander SK (2001) Molecular genetic analysis of virulence in Mannheimia (Pasteurella) haemolyiica. Front biosci 6:1J 28-1150
Hounsome JD, Baillie S, Noofeli M, Riboldi-Tunnicliffe A, Burchmore RJ, Isaacs NW, Davies RL (2011) Outer membrane protein A of bovine and ovine isolates of Mannheimia haemolyiica is surface exposed and contains host species-specific epitopes. Infect Immun 79:4332^4-341.
Jukes TH, Cantor CR (1969) Evolution of protein molecules, In: Mammalian Protein Metabolism Academic Press, New York: pp 21-132
Kisiela DI, Czuprynski CJ (2009) Identification of Mannheimia haemolyiica adhesins involved in binding to bovine bronchial epithelial cells. Infect Immun 77:446-455.
Lawrence PK, Kiuiehotirat W, McDermott JE, Bumgarner RE (2010) A three-way comparative genomic analysis of Mannheimia haemolyiica isolates, BMC Genomics 11:535
Lo RY, Sorcnscn LS (2007) The outer membrane protein OmpA of Mannheimia haemolyiica Al is involved in the binding of fibronectin. FEMS Microbiol Lett 274:226-231
McAuIiffe L, Ellis RJ, Ayling RD and Nicholas RA (2003) Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting. J Clin Microbiol 41:4844-4847.
Muyzer G, and Smalla Κ (1998) Application of denaturing gradient gel electrophoresis (UGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Lecuwenhoek 73:127-141.
Nielsen EM, Enberg J, Fussing V, Petersen L, Brogen CH and On SL (2000) Evaluation of phenotypic and genotypic methods for sub typing Campylobacter jejuni isolales from humans, poultry, and cattle, J Clin Microbiol 38:3800-3810
Puohiniemi R, Karvonen M, Vuopio-Varkila J, Muoliala A, Helander IM, Sarvas M (1990) A strong antibody response to the periplasmic C-terminal domain of the OmpA prolein of Escherichia coli is produced by immunization with purified OmpA or with whole E. coli or Salmonella typhimurium bacteria. Infect Immun 58:1691-1696
Sailou N, Nei M ( 1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol Biol Evol 4:406-425
Sheffield VC, Cox DR, Lerman LS and Myers RM (1989) Attachment of a 40-base-pair G + C-rich sequenze (GC-clamp) lo genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes, Ρ Natl Acad Sci USA 86:232-236
Tamura K, Dudley J, Nei M, Kumar S (2007) MÏÎGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol E vol 24:1596-1599.
Thompson JD, Higgins DG, Gibson Τ J (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res 22:4673-46 SCI.
Untergasser A, Nijven H, Rao X, Bisseling T, Geurts R, Leunissen JA (2007) Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res 35: W71-74
Villard L, Gauthier D, Lacheretz A, Abadie G, Game Y, Maurin F, Richard Y, Borges Π, Kodjo A (2006) Serological and molecular comparison of Mannhehnia haemoiyiica and Pasteurella trehaiosi strains isolated from wild and domestic ruminants in the French Alps. Vet J 171:545-550
Younan M, Fodar L {1995) Characterisation of a new Pasteurella haemolytica serotype (Al7). Res Vet Sci 58:98.
Zecchinon L, Fett T, Desmecht 13 (2005) How Mannheimia haemolytica defeats host defence through a kiss of death mechanism. Vet Res 36:133-156
Zeng H, Pandher K, Murphy GL (1999) Molecular cloning of the Pasteurella haemolytica pomA gene and identification of bovine antibodies against Pom A surface domains. Infect Immun 67:4968-497.1.