Leptospira spp. and Brucella ovis seroprevalence in sheep: preliminary results of one year surveillance program

PDF (English)
DOI: https://doi.org/10.12681/jhvms.16053
F. BERTELLONI
Department of Veterinary Sciences, University of Pisa
B. TURCHI
Department of Veterinary Sciences, University of Pisa
D. CERRI
Department of Veterinary Sciences, University of Pisa
P. PINZAUTI
Department of Veterinary Sciences, University of Pisa
F. FRATINI
Department of Veterinary Sciences, University of Pisa
Abstract

In order to assess the diffusion of brucellosis by Brucella ovis and leptospirosis in sheep flocks in Tuscany, 410 blood samples were collected from males of 76 breeding farms from January to December 2015. All sera resulted negative for B. ovis. A percentage of 17.80% sera was found positive for Leptospira spp. Among all breeding farms, 34.21% resulted positive. The most represented serovars were Pomona (6.34%), Hardjo (4.14%), Grippotyphosa (3.17%) and Bratislava (1.70%). The highest antibody titers were detected for serovar Hardjo (1:25600) and serovars Pomona and Grippotyphosa (1:1600). These results confirm the role of sheep as maintenance host for serovar Hardjo and highlight the spreading of serovar Grippotyphosa in the study area. Constant field investigation, especially on farm animals, could be useful to determine trends and diffusion of some occupational re-emerging diseases, such as leptospirosis.

Article Details
  • Sezione
  • Research Articles
Creative Commons License

Questo lavoro è fornito con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
Adler B, de la Peña Moctezuma A (2010) Leptospira and leptospirosis. Vet Microbiol 140(3-4):287-96.
Andreoli E, Radaelli E, Bertoletti I, Bianchi A, Scanziani E, Tagliabue S, Mattiello S (2014) Leptospira spp. infection in wild ruminants: a survey in Central Italian Alps. Vet Ital 50(4):285-91.
Ayral FC, Bicout DJ, Pereira H, Artois M, Kodjo A (2014) Distribution of Leptospira serogroups in cattle herds and dogs in France. Am J Trop Med Hyg 91(4):756-9.
Cerri D, Ebani VV, Fratini F, Pinzauti P, Andreani E (2003) Epidemiology of leptospirosis: observations on serological data obtained by a “diagnostic laboratory for leptospirosis” from 1995 to 2001. New Microbiol 26(4):383-9.
Cerri D, Ebani VV, Pedrini A, Bassi S, Bey RF, Andreani E, Farina R (2000) Evaluation of tests employed in serological diagnosis of brucellosis caused by Brucella ovis. New Microbiol 23(3):281-8.
Cerri D, Ebani VV, Pedrini A, Nuvoloni R, Renzoni G, Andreani E, Farina R (1999) Epididymitis by Brucella ovis: experimental infection in virgin ram lambs. New Microbiol 22(3):227-31.
Ciceroni L, Lombardo D, Pinto A, Ciarrocchi S, Simeoni J (2000) Prevalence of antibodies to Leptospira serovars in sheep and goats in Alto Adige-South Tyrol. J Vet Med B Infect Dis Vet Public Health 47(3):217-23.
de Bagüés JMP, Barberán M, Marín CM, Blasco JM (1995) The Brucella abortus RB51 vaccine does not confer protection against Brucella ovis in rams. Vaccine 13(3):301-4.
de Carvalho SM, Mineiro AL, Castro V, Genovez ME, Azevedo SS, Costa FA (2014) Leptospirosis seroprevalence and risk factors for sheep in Maranhão state, Brazil. Trop Anim Health Prod 46(2):491-4.
De Massis F, Di Provvido A, Di Sabatino D, Di Francesco D, Zilli K, Ancora M, Tittarelli M (2012) Isolation of Brucella suis biovar 2 from a wild boar in the Abruzzo Region of Italy. Vet Ital 48(4):397-404.
DGR 1204/2009: Delibera della Giunta Regionale N° 1204 del 21-12- 2009: Modifica delle linee guida per la profilassi e l’eradicazione della tubercolosi bovina, brucellosi bovina, brucellosi ovi-caprina e leucosi bovina enzootica, Bollettino Ufficiale Della Regione Toscana, N. 52, 30/12/2009 (Italian).
Director A, Penna B, Hamond C, Loureiro AP, Martins G, Medeiros MA, Lilenbaum W (2014) Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission. J Med Microbiol
(Pt 9):1234-6.
Farina R, Cerri D, Renzoni G, Andreani E, Mani P, Ebani V, Pedrini A, Nuvoloni R (1996) Leptospira interrogans in the genital tract of sheep. Research on ewes and rams experimentally infected with serovar hardjo (hardjobovis). New Microbiol 19(3):235-42.
Lilenbaum W, Varges R, Ristow P, Cortez A, Souza SO, Richtzenhain LJ, Vasconcellos SA (2009) Identification of Leptospira spp. carriers among seroreactive goats and sheep by polymerase chain reaction. Res Vet Sci 87(1):16-9.
Lopez G, Escobar GI, Ayala SM, Lucero NE (2006) Detection of antibodies to Brucella ovis in sheep milk using B. ovis and B. canis antigen. Vet Microbiol 116(1-3):232-8.
Magajevski FS, Girio RJS, Mathias LA, Myashiro S, Genovez MÉ, Scarcelli EP (2005) Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo. Braz J Microbiol 36(1):43-45.
Martins G and Lilenbaum W (2014) Leptospirosis in sheep and goats under tropical conditions. Trop Anim Health Prod 46(1):11-7.
Melo LDSS, Castro MBD, Leite RC, Moreira ÉC, Melo CBD (2010) Main aspects of Leptospira sp infection in sheep. Cienc Rural 40(5):1235-1241.
Petrović M, Špičić S, Potkonjak A, Lako B, Kostov M, Cvetnić Ž (2014) First evidence of Brucella ovis infection in rams in the Pirot Municipality, Serbia. Vet Ital Dec 29;50(4):259-68.
Picardeau M (2013) Diagnosis and epidemiology of leptospirosis. Med Mal Infect 43(1):1-9.
Picard-Hagen N, Berthelot X, Champion JL, Eon L, Lyazrhi F, Marois M, Peglion M, Schuster A, Trouche C, Garin-Bastuji B (2015) Contagious epididymitis due to Brucella ovis: relationship between sexual function, serology and bacterial shedding in semen. BMC Vet Res May 30;11:125.
Spicić S, Marjanović S, Zdelar-Tuk M, Cvetnić Z (2009) First evidence of Brucella ovis infection in Republic of Croatia. Dtsch Tierarztl Wochenschr Jun;116(6):209-13.
Tonin AA, Martins B, Zago RVMS, Tochetto C, Azenha NP, Schaefer PC, Badke MRT (2015) Outbreak of leptospirosis: reproductive losses in sheep. Comp Clin Path 24(4):961-965.
Treml F, Pikula J, Bandouchova H, Horakova J (2007) European brown hare as a potential source of zoonotic agents. Veterinarni Medicina-Praha 52(10):451-456.
Żmudzki J, Jabłoński A, Nowak A, Zębek S, Arent Z, Bocian Ł, Pejsak Z (2016) First overall report of Leptospira infections in wild boars in Poland. Acta Vet Scand 58(1):3.
Puoi leggere altri articoli dello stesso autore/i