Heavy Metal Residues and Microbial Status of Farmed and Channeled Cat Fish

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Pubblicato: Apr 19, 2024
DOI: https://doi.org/10.12681/jhvms.33294
NA Ebied
animal health research institute, kafrelsheikh university
https://orcid.org/0000-0002-0833-9944
MS Abdou
Bacteriology department, Animal Health Research Institute, Kafr El-Sheikh branch, Agricultural Research Center (ARC), Egypt
ME Abass
Biochemistry department, Animal Health Research Institute, Kafr El-Sheikh branch, Agricultural Research Center (ARC), Egypt
AS Kassab
Fish diseases department, Animal Health Research Institute, Kafr El-Sheikh branch, Agricultural Research Center (ARC), Egypt
Abstract

The purpose of the study was to look into the bioaccumulation of Cu, Cd, Pb, Mn, Fe and Zn in the muscles and livers of Clarias gariepinus catfish from farms and channels sources of River Nile in Kafr El-Sheikh province, Egypt, with microbiological evaluation of fish quality, and  detection of Shiga toxin genes (stx1, stx2), and metal resistant genes (MRGs) in E. coli isolated and identified as Shiga toxin-producing E. coli (STEC) from fish muscles to predict the crucial hazards on consumers’ health from fish of both sources. The results showed increased metal levels in the tissues and liver of farmed fish than in the channeled, especially Pb detected by very high concentrations (16.39±1.26 and 11.90±1.68) mg/kg in 20% of examined muscles and liver samples of farmed fish, all detected levels of Pb were exceeded the permissible limits (PLs). Pb was not detected in the channel source. Mn, Fe and Zn were detected in all samples of both sources by (100%), but Mn and Fe only exceeded the PLs, while Zinc was with values lower than PLs. The (TBC), (TCC), Contamination rates with E.coli, STEC, and E.coli with (nccA and czcD) were found also significantly higher (p < 0.05) in examined muscle samples from farmed than channeled source. Stx2, nccA, and czcD genes were detected by 10%, 30%, and 20% respectively from identified STEC samples. In conclusion, farmed catfish had higher levels of heavy metals and bacterial pollution than channel catfish,in addition metal resistant bacteria may be considered an indicator of heavy metal contamination in the aquatic ecosystem

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Biografia autore
NA Ebied, animal health research institute, kafrelsheikh university
  • phd. food hygiene
  • Senior Researcher at Animal Health Research Institute
Kafr ash Shaykh, Egypt food safety, hygiene, control, and quality. ISO 9001, HACCP, GMP. lead auditor FSMS ISO 22OOO & FSSC 22000/ ERCA.
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