Antioxidant and antiproliferative effects of different Raphanus Sativus L. extracts
Abstract
This study aimed to investigate the phenolic and flavonoid levels, antioxidant activities and antiproliferative effects of Japanese, Chinese, Black, and Kohlrabi radish species on HEK293 immortalized human embryonic kidney cells in cell culture medium. DPPH radical scavenging activity, total polyphenol, and flavonoid content of the extracts were measured spectrophotometrically. The highest total flavonoid content in sap of radish extraction and methanolic extraction was 0.12±0.02 mg/g RE and 0.10±0.00 mg/g RE in Chinese radish; the highest total polyphenol content was 0.32±0.03 mg/g GAE and 0.23±0.03 mg/g GAE in Black radish; the DPPH radical scavenging activity is approved 39.68±2.43 µg/g GAE in Japanese radish in sap of radish extraction and 84.59±3.50 µg/g GAE in Black radish in methanolic extraction respectively. DPPH radical scavenging activity was higher in the methanolic extraction of all radishes. The antiproliferative effects of different extracts in HEK293 cells were investigated by the MTT method. The IC50 concentration was found at lower doses in the methanolic extraction of whole radishes compared to the sap of radish extractions. The lowest dose of antiproliferative effect was observed in red radish (160 mg/ml) in methanolic extraction, and the antiproliferative effect increased dose-dependent. As a result, both antioxidant content and antiproliferative effect may vary depending on the type of radish, soil, and geographical conditions of the region where it is grown.
Article Details
- Come citare
-
Tiraş, S., Kaya Kartal, Y., Ercan, N., & Sel, T. (2024). Antioxidant and antiproliferative effects of different Raphanus Sativus L. extracts. Journal of the Hellenic Veterinary Medical Society, 75(3), 7757–7764. https://doi.org/10.12681/jhvms.35433
- Fascicolo
- V. 75 N. 3 (2024)
- Sezione
- Research Articles
Questo lavoro è fornito con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
