Rock salt and boron supplementation: Effects on oxidative stress in blood and semen of rams

PDF (English)
Pubblicato: Δεκ 17, 2025
DOI: https://doi.org/10.12681/jhvms.40490
S Akarsu
Fırat University, Faculty of Veterinary Medicine, Department of Animal Science, Elazığ, Turkey
ÜG Şimşek
Fırat University, Faculty of Veterinary Medicine, Department of Animal Science, Elazığ, Turkey
M Erişir
Fırat University, Faculty of Veterinary Medicine, Department of Biochemistry, Elazığ, Turkey
M Sönmez
Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey
İH Güngör
Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey
TC Acisu
Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey
M Erdem
Turgut Özal Unıversity, Faculty of Medicine, Department of Medical Biochemistry, Malatya, Turkey
MA Şimşek
Dokuz Eylül University, Faculty of Veterinary Medicine, Department of Animal Science, İzmir, Turkey
EN Elçi
Fırat University, Faculty of Veterinary Medicine, Department of Biochemistry, Elazığ, Turkey
Abstract

This study aimed to investigate the effects of boron and rock salt minerals, which are interesting as antioxidant minerals on malondialdehyde (MDA) and some antioxidants; glutathione level (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) enzyme activities in blood and sperm cells. For this purpose, a total of 36 rams were divided into six research groups, with six rams in each group. The research groups were designed as Control (Just 40 ml water), Salt (10 g rock salt/day), B20 (20 mg boric acid/day), B40 (40 mg boric acid/day), BT20 (20 mg boric acid + 10 g rock salt/day), BT40 (20 mg boric acid + 10 g rock salt/day). The additives were dissolved in 40 ml of water and administered orally with 50 ml syringes for 80 days. Semen and blood samples were taken from the rams at the end of the experiment. Salt added to the diet significantly increased MDA levels in blood and sperm cells. A significant decrease in plasma MDA levels were observed in the BT40 group and sperm cells in the B40 and BT40 groups. Salt and boric acid were found to be effective on GSH and antioxidant enzymes. In conclusion, it was found that boric acid added to the diet at a dose of 40 mg enhanced antioxidant metabolism and significantly reduced salt-induced lipid peroxidation.

Article Details
  • Sezione
  • Research Articles
Creative Commons License

Questo lavoro è fornito con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
AL-Enzy AFM, Saed ZJ, Naser AS, Mohammed TT, Abdulateef SM,
AL-Khalani FMH, Abdulateef, FM (2020) The Role of Adding
Sodium Chloride in Broiler Chicks Diets to Improve Production
Performance and Antioxidant Status during Heat Stress. Ann Trop
Med Public Health 23(S16): SP231612.
Avdatek F, Demirel HH, Küçükkurt İ, Acaröz DA, İnce S (2022) The
effects of boron on oxidative stress, reproductive parameters, and
DNA damage in testicular tissue in wistar rats on a fatty diet. J
Hellenic Vet Med Soc 73(4):4729-4738.
Ayşan E, Idiz UO, Şahin F (2024) Radiant Defense: Harnessing Boron-
Based Gel for Shielding Against Radiation-Induced Dermatitis
in Rat Models. Cerasus J Med 1(2):131-138.
Barbagallo F, La Vignera S, Cannarella R, Aversa A, Calogero AE,
Condorelli RA (2020) Evaluation of sperm mitochondrial function:
a key organelle for sperm motility. J Clin Med 9(2):363.
Cakir S, Eren M, Senturk M, Sarica ZS (2018) The effect of boron on
some biochemical parameters in experimental diabetic rats. Biol
Trace Elem Res 184:165-172.
Cengiz M, Sahinturk V, Yildiz SC, Şahin İK, Bilici N, Yaman SO,
Altuner Y, Ayhanci (2020) A Cyclophosphamide induced oxidative
stress, lipid per oxidation, apoptosis and histopathological changes
in rats: Protective role of boron. J Trace Elem Med Biol 62:126574.
Chavan S, Sava L, Saxena V, Pillai S, Sontakke A and Ingole D (2005)
Reduced Glutathione: Importance of specimen collection. I J of
Clin Biochem 20(1):150-152.
Cordiano R, Di Gioacchino M, Mangifesta R, Panzera C, Gangemi S,
Minciullo PL. Malondialdehyde as a Potential Oxidative Stress
Marker for Allergy-Oriented Diseases: An Update. Molecules.
Aug 9;28(16):5979. doi: 10.3390/molecules28165979. PMID:
; PMCID: PMC10457993.
Corino C, Rossi R (2021) Antioxidants in animal nutrition. Antioxidants
(12):1877.
Cortés S, Espinoza-Navarro O, Ferreccio C (2017) High Exposure to
Boron in Drinking Water and Sperm Parameters in Chilean Young
People. Int J Morphol 35(1):99-104.
El-Saadany AS, EL-Barbary AM, Shreif EY (2016) The influence of
dietary boron supplementation on performance and some physiological
parameters in Bandarah chickens 1-growing period. Egypt
Poult Sci J 36:4.
Estevez-Fregoso E, Kilic A, Rodríguez-Vera D, Nicanor-Juárez LE,
Romero-Rizo CEM, Farfán-García ED, Soriano-Ursúa MA (2023)
Effects of boron-containing compounds on liposoluble hormone
functions. Inorganics 11(2):84.
Góth L (1991) A simple method for determination of serum catalase
activity and revision of reference range. Clin Chim Acta 196:143-5.
Gu YF, Wu DQ, Chen HL, Wang J, Shang CF, Liu DY (2007) Effect
of boron on free radical in serum of chickens. Stud Trace Elem
Health 5:003.
Ince, S., Kucukkurt, I., Demirel, H. H., Arslan-Acaroz, D., & Varol,
N. (2020). Boron, a trace mineral, alleviates gentamicin-induced
nephrotoxicity in rats. Biological trace element research, 195(2),
-524.
Kalaçay D, Atakisi O (2022) Investigation the Effect of Boric Acid
effect on Antioxidant System, HDL Levels and PON Activity on
Rats Feding to the High-Fat Diet Cauc J Sci 9(1):49-6.
Kan F, Kucukkurt I (2023) The effects of boron on some biochemical
parameters: A review. J Trace Elem Med Biol 79:127249.
Kandemir Ç, Taşkın T (2022) Türkiye’de Koyun Irklarının Mevcut
Durumu ve Geleceği. Akdeniz Bölgesi Türk Tarım Araşt Derg
(1):97-106.
Khaliq H, Juming Z, Ke-Mei P (2018) The physiological role of boron
on health. Biol Trace Elem Res 186:31-51.
Krishnan BB, Selvaraju S, Gowda NKS, Subramanya KB, Pal D, Archana
SS, Bhatta R (2019) Dietary boron supplementation enhances
sperm quality and immunity through influencing the associated
biochemical parameters and modulating the genes expression at
testicular tissue. J Trace Elem Med Biol 55:6-14.
Kucukkurt I, Ince S, Demirel HH, Turkmen R, Akbel E, Celik Y (2015)
The Effects of Boron on Arsenic‐Induced Lipid Peroxidation and
Antioxidant Status in Male and Female Rats. J Biochem Mol Toxicol
(12):564-571.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurements
with the folin phenol reagent. J Biol Chem 193:265-275.
Mahmood N, Hameed A, Hussain T (2020) Vitamin E and Selenium
Treatment Alleviates Saline Environment‐Induced Oxidative Stress
through Enhanced Antioxidants and Growth Performance in Suckling
Kids of Beetal Goats. Oxid Med Cell Longev 1:4960507.
Matkovics B, Szabo I, Varga IS (1988) Determination of enzyme activities
in lipid peroxidation and glutathione pathways. Lab Diagn
:248–249.
Mohamed RS, Mohamed RH, Wehrend A, Tahoun EA, Hussein HA
(2024) The effects of saline water consumption on sperm parameters,
testicular histopathology, hormonal and antioxidants concentrations
in Barki Rams. BMC Vet Res 20(1):219.
Mohammed RS, Donia GR, Tahoun EA, El Ebissy EA (2019) Oxidative
Stress and Histopathological Alternations in Sheep as A Result of
Drinking Saline Water Under the Arid Conditions of Southern Sinai,
Egypt. Alex J Vet Sci 61:1.
Naim Z, Asaduzzaman M, Akter M, Islam MS (2023) Impact of climate
change on livestock production in Bangladesh-A review. Bangl J
Anim Sci 52(1):1-14.
Nielsen FH (2017) Historical and recent aspects of boron in human and
animal health. J. Boron 2(3):153-160.
Özdemir G, İnci H, Söğüt B, Şengül T, Yüksel H, Şimşek H, Özdemir
A (2016) Effects of dietary boron supplementation on performance
and some haematological and antioxidant parameters in Japanese
quail exposed to high stocking density. Eur Poult Sci or Arch Geflügelkd
:137.
Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of
lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal
Biochem 16:359-364.
Ponnampalam, E. N., Kiani, A., Santhiravel, S., Holman, B. W., Lauridsen,
C., & Dunshea, F. R. (2022). The importance of dietary
antioxidants on oxidative stress, meat and milk production, and their
preservative aspects in farm animals: Antioxidant action, animal
health, and product quality—Invited review. Animals, 12(23), 3279.
Rankins Jr DL, Pugh DG (2011) Feeding and Nutrition. In: Sheep &
Goat Medicine-E-Book. 2nd ed, Elsevier, Missouri: pp 18-49.
Salazar-Hernández, E., Bahena-Cuevas, O. E., Mendoza-Bello, J. M.,
Barragán-Bonilla, M. I., Sánchez-Alavez, M., & Espinoza-Rojo, M.
(2025). Relationship Between Brain Insulin Resistance, Carbohydrate
Consumption, and Protein Carbonyls, and the Link Between
Peripheral Insulin Resistance, Fat Consumption, and Malondialdehyde.
Biomedicines, 13(2), 404.
Silvestrini A, Meucci E, Ricerca BM, Mancini A (2023) Total antioxidant
capacity: biochemical aspects and clinical significance. Int J
Mol Sci 24(13):10978.
Simsek UG, Karabulut B, Kaya SO, Erişir M, Baykalir Y, Aslan S,
Cevik A, Kahramanogullari M (2024) The stimulatory effects of
boron on Japanese quail spermatological activity, histopathology,
and oxidative stress. Acta Veterinaria Brno 93(2):209-218.
Sogut I, Oglakci A, Kartkaya K, Ol KK, Sogut MS, Kanbak G, Inal
ME (2015) Effect of boric acid on oxidative stress in rats with fetal
alcohol syndrome. Exp Ther Med 9(3):1023-1027.
Sonawane H, Shelke D, Arya S, Ghole V, Behra B, Gaikwad S (2024)
Hypoglycemic and hepatoprotective activity of Phellinus fastuosus
on streptozotocin-induced diabetic rats and carbon tetrachloride-intoxicated
rats, respectively. Future J Pharm Sci 10(1):79.
Sun Y, Oberley WL, Lı Y (1988) A simple method for clinical assay of
superoxide dismutase. Clin Chem 34:497-500.
Surai, P. F., Earle-Payne, K. (2022). Antioxidant defences and redox
homeostasis in animals. Antioxidants, 11(5), 1012.
Tirascı, N., Simsek, U. G. (2024). The impacts of distinct light spectra
on the growth properties and maternal productivity of rats. Journal
of the Hellenic Veterinary Medical Society, 75(4), 8371-8380.
Turkez H, Geyikoglu F, Tatar A, Keles S, Ozkanc A (2007) Effects of
some boron compounds on peripheral human blood. Z Naturforsch
:889-896.
Washington IM, Van Hoosier G (2012) Clinical biochemistry and hematology.
In: The Laboratory Rabbit, Guinea Pig, Hamster, And Other
Rodents. 1st ed, Acad Press, Washington: pp: 57-116.
Yeni D, Gündoğan M (2018) Koçlarda bazı androlojikal parametrelerin
ve biyokimyasal özelliklerin mevsimle ilişkisi. Kocatepe Vet J
(1):70-85.
Zhao C, Chen S, Han Y, Zhang F, Ren M, Hu Q, Ye P, Li X, Jin E, Li
S (2023) Proteomic analysis of rat duodenum reveals the modulatory
effect of boron supplementation on immune activity. Genes
(8):1560.
Puoi leggere altri articoli dello stesso autore/i