Effects of Curcumin and Nanocurcumin on Biochemical Parameters, and Performance of Broiler Chickens under Physiological Stress
Abstract
This study aimed to evaluate the effects of curcumin and nanocurcumin on growth performance, antioxidant status, inflammatory response, and hematological changes in male broiler chickens subjected to experimentally induced stress via in-feed dexamethasone (DEX). A total of 400 one-day-old male Ross 308 chicks were randomly assigned to four groups: (1) a positive control (PC) group with no treatment, (2) a negative control (NC) group receiving 1.5 mg/kg DEX, (3) a group receiving 1.5 mg/kg DEX and 200 mg/kg curcumin (Cur), and (4) a nanocurcumin (Nano-Cur) group receiving 1.5 mg/kg DEX and 200 mg/kg nanocurcumin. Each group consisted of five replicates with 20 birds per replicate. Dietary supplementation with curcumin or nanocurcumin mitigated the adverse effects of DEX on body weight, feed conversion ratio, and mortality. During the stress period, Nano-Cur supplementation enhanced serum superoxide dismutase and glutathione peroxidase activity while reducing malondialdehyde levels. Additionally, Cur/Nano-Cur supplementation lowered serum total cholesterol, triglycerides, aspartate aminotransferase (AST), and alanine transaminase (ALT) levels, while increasing IgG and IgM compared to the NC group. Physiological stress affected serum levels of TNF-α and IL-8, while DEX increased IL-10 levels. However, dietary supplementation with curcumin or nanocurcumin reduced TNF-α and increased IL-8 and IL-10 levels. Furthermore, curcumin/nanocurcumin supplementation alleviated the adverse effects of DEX by decreasing heterophil counts and the heterophil-to-lymphocyte ratio, while increasing lymphocyte counts, hematocrit (HCT), and hemoglobin (HGB) levels. In conclusion, dietary curcumin and nanocurcumin supplementation can mitigate DEX-induced oxidative stress and inflammatory responses, supporting their potential role in improving stress resilience in broiler chickens
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Fathi, M., Saidian , S., Baghaeifar , Z., Mardani, P., Zandiyah, Z., Mansory, G., & Rezaee, V. (2026). Effects of Curcumin and Nanocurcumin on Biochemical Parameters, and Performance of Broiler Chickens under Physiological Stress. Journal of the Hellenic Veterinary Medical Society, 77(1), 10135–10144. https://doi.org/10.12681/jhvms.41130
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- V. 77 N. 1 (2026)
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Riferimenti bibliografici
Adedapo AA, Saba AB, Dina OA, Oladejo GMA. 2004. Effects of dexamethasone
on the infectivity of Trypanosoma vivax and serum
biochemistry change in Nigerian domestic chickens. Veterinarski
arhiv Trop J Anim Sci 74(5): 371–382.
Abd El-Hack, M. R., Bothaina A, Alaidaroos, Reem M. Farsi, Diaa E.
Abou-Kassem Mohamed T. El-Saadony, Ahmed M. Saad, Manal E.
Shafi, Najah M. Albaqami, Ayman E. Taha and 6. Elwy A. Ashour.
Impacts of Supplementing Broiler Diets with Biological Curcumin,
Zinc Nanoparticles and Bacillus licheniformis on Growth,
Carcass Traits, Blood Indices, Meat Quality and Cecal Microbial
Load. Anim 11, 1878.
Attia YA, Abd El AE, Abedalla AA, Berika MA, Al-Harthi MA, Kucuk
O, Sahin K, Abou-Shehema BM. 2016. Laying performance, digestibility
and plasma hormones in laying hens exposed to chronic
heat stress as affected by betaine, vitamin C, and/or vitamin E
supplementation. Springerplus 5(1):1619.
Attia YA, Al-Harthi MA, Hassan SS. Turmeric (Curcuma longa Linn.)
as a phytogenic growth promoter alternative for antibiotic and comparable
to mannan oligosaccharides for broiler chicks. Rev Mex
Cienc Pecu 2017; 8(1): 11-21.
Attia YA, Al-Harthi MA, Sh. Elnaggar A. 2018. Productive,
physiological and immunological responses of two broiler
strains fed different dietary regimens and exposed to heat
stress. Ital J Anim Sci 17(3):686–697.
Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. 2007. Bioavailability
of curcumin: problems and promises. Mol Pharm
:807–818.
Arab HA, Jamshidi R, Rassouli A, Shams G, Hassanzadeh MH. 2006.
Generation of hydroxyl radicals during ascites experimentally induced
in broilers. Bri Poult Sci. 47:216–222
Al-Sultan SI, Gameel AA. 2004. Histopathological changes in the livers
of broiler chicken supplemented with turmeric (Curcuma longa).
Int J Poult Sci 3:333–336.
Awad EA, Najaa M, Abidin Z, Zulkifli I, Soleimani AF. 2020. Effects
of heat stress on growth performance, selected physiological and
immunological parameters, caecal microflora, and meat quality in
two broiler strains. Asian-Australas J Anim Sci 33(5): 778–787.
Al wakeel A. Al wakeel, Mustafa Shukry, Ahmed Abdel Azeez, Shawky
Mahmoud & Michel Fahmy Saad. 2017. Alleviation by gamma
amino butyric acid supplementation of chronic heat stress-induced
degenerative changes in jejunum in commercial broiler chickens.
Stress 20 (6)562–572 https://doi.org/10.1080/10253890.2017.13
Barzegar Yarmohammadi A., Sharifi, S.D., Mohammadi-Sangcheshmeh,
A. 2020. Efficacy of dietary supplementation of nanoparticles-chromium,
chromiummethionine and zinc-proteinate, on performance of
Japanese quail under physiological stress. Ital jou of anim sci 19 (1),
–1134. DOI: https://doi.org/10.1080/1828051X.2020.1822763.
Cleary K. 2004. Effects of oxygen and turmeric on the formulation of
oxidative aldehyde in freshpack dill pickles [A Thesis submitted
to the Graduate Faculty of North Carolina].
Dejager L, Vandevyver S, Petta I, Libert C. 2014. Dominance of the
strongest: inflammatory cytokines versus glucocorticoids. Cytokine
Growth Factor Rev 25: 21–33.
Donatus IA, Sardjoko IA, Vermeulen NPE. 1990. Cytotoxic and cyto -
protective activities of curcumin. Effects on paracetamol-induced
cytotoxicity, lipid peroxidation and glutathione depletion in rat
hepatocytes. Biochem Pharmacol 39: 1869–1875.
Eid Y, Ohtsuka A, Hayashi K. 2003. Tea polyphenols reduce glucocorticoid-
induced growth inhibition and oxidative stress in broiler
chickens. Br Poult Sci 44(1): 127–132.
Eladl AH, Arafat N, El-Shafei RA, Farag VM, Saleh RM, Awadin WF.
Comparative immune response and pathogenicity of the H9N2
avian influenza virus after administration of Immulant®, based on
Echinacea and Nigella sativa, in stressed chickens. Comparative
Immunology, Microbiology and Infectious Diseases 65: 165–175.
Fathi M, Tanha T, Saeedyan S. 2022. Influence of dietary lycopene
on growth performance, antioxidant status, blood parameters and
mortality in broiler chicken with cold-induced ascites. Arch Anim
Nutr 8: 1–11. doi: 10.1080/1745039X.2022.2046451.
References
Fathi M, Saeedyan S, Kaoosi M. 2023. Gamma-amino butyric acid
(GABA) supplementation alleviates dexamethasone treatment-induced
oxidative stress and inflammation response in broiler chickens.
Stress 26(1): 2185861. DOI: 10.1080/10253890.2023.2185861.
Furukawa K, Kikusato M, Kamizono T, Toyomizu M. 2016. Time-course
changes in muscle protein degradation in heat-stressed chickens:
Possible involvement of corticosterone and mitochondrial reactive
oxygen species generation in induction of the ubiquitin–proteasome
system. Gen Comp Endocrinol 228: 105–110.
Galha V, Bondan EF, Lallo MA. 2008. Relationship between immunosuppression
and clinical coccidiosis in commercially raised broilers.
Rev Inst Cienc Health 26: 432–437.
Gao J, Lin H, Song ZG, Jiao HC. 2008. Corticosterone alters meat
quality by changing pre- and post-slaughter muscle metabolism.
Poult Sci 87: 1609–1617.
Gao J, Lin H, Wang XJ, Song ZG, Jiao HC. 2010. Vitamin E supplementation
alleviates the oxidative stress induced by dexamethasone
treatment and improves meat quality in broiler chickens. Poult Sci
: 318–327.
Gorabi AM, Razi B, Aslani S, Abbasifard M, Imani D, Sathyapalan
T, Sahebkar A. 2021. Effect of curcumin on proinflammatory cytokines:
A meta-analysis of randomized controlled trials. Cytokine
: 155541. DOI: 10.1016/j.cyto.2021.155541.
Galati G, Sabzevari O, Wilson JX, O’Brien PJ. 2002. Prooxidant activity
and cellular effects of the phenoxyl radicals of dietary flavonoids
and other polyphenolics. Toxicol 177: 91–104.
Gautam SC, Gao X, Dulchavsky S. 2007. Immunomodulation by curcumin.
Adv Exp Med Biol 595: 321–341.
Churchill M, Chadburn A, Bilinski RT, Bertagnolli MM. 2000. Inhibition
of intestinal tumors by curcumin is associated with changes in the
intestinal immune cell profile. J Surg Res 89: 169–175.
Ghanima MMA, Abd El-Hack ME, Othman SI, Taha AE, Allam AA,
Abdel-Moneim AME. 2020. Impact of different rearing systems
on growth, carcass traits, oxidative stress biomarkers, and humoral
immunity of broilers exposed to heat stress. Poult Sci 99(6):
–3078.
Haldar S, Ghosh T, Bedford M. 2011. Effects of yeast (Saccharomyces
cerevisiae) and yeast protein concentrate on production performance
of broiler chickens exposed to heat stress and challenged
with Salmonella enteritidis. Anim Feed Sci Technol 168: 61–71.
Hernandez F, Madrid J, Garcia V, Orengo J, Megias MD. 2004. Influence
of two plant extracts on broilers performance, digestibility, and
digestive organ size. Poult Sci 83: 169–174.
Houshmand M, Azhar K, Zulkifli I, Bejo MH, Kamyab A. 2012. Effects
of prebiotic, protein level, and stocking density on performance,
immunity, and stress indicators of broilers. Poult Sci 91: 393–401.
Heidary M, Hassanabadi A, Mohebalian H. 2020. Effects of in ovo
injection of nanocurcumin and vitamin E on antioxidant status,
immune responses, intestinal morphology and growth performance
of broiler chickens exposed to heat stress. J Liv Sci Techno 8(1):
–27. DOI: 10.22103/jlst.2020.15352.1310.
Huang WT, Niu KC, Chang CK, Lin MT, Chang CP. 2008. Curcumin
inhibits the increase of glutamate, hydroxyl radicals and PGE2 in
the hypothalamus and reduces fever during LPS-induced systemic
inflammation in rabbits. Eur J Pharmacol 593: 105–111. DOI:
1016/j.ejphar.2008.07.017.
Huang H, Chen J, Sun Q, Teng X. 2021. NLRP3 inflammasome is
involved in the mechanism of mitigative effect of selenium on
lead-induced inflammatory damage in chicken kidneys. Environ
Sci Pollut Res 28(9): 1–11.
Jang IS, Ko YH, Moon YS, Sohn SH. 2014. Effects of vitamin C or
E on the pro-inflammatory cytokines, heat shock protein 70 and
antioxidant status in broiler chicks under summer conditions.
Asian-Australas J Anim Sci 27(5): 749–760.
Lin H, Buyse J, Decuypere E. 2006a. Strategies for prevention of heat
stress in poultry. Worlds Poult Sci J 62: 71–85.
Lin H, Decuypere E, Buyse J. 2006b. Acute heat stress induces oxidative
stress in broiler chickens. Comp Biochem Physiol A Mol Integr
Physiol 144: 11–17.
Lin H, Decuypere E, Buyse J. 2004. Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus)
chronic exposure. Comp Biochem Physiol 139: 737–744.
Li Y, Cai HY, Liu GH, Dong XL, Chang WH, Zhang S, Zheng AJ, Chen
GL. 2009. Effects of stress simulated by dexamethasone on jejunal
glucose transport in broilers. Poult Sci 88(2): 330–337.
Lv ZP, Peng YZ, Zhang BB, Fan H, Liu D, Guo YM. 2018. Glucose
and lipid metabolism disorders in the chickens with dexamethasone-
induced oxidative stress. J Anim Phys Anim Nutr 102(2): e706.
Luo JW, Zhou ZL, Zhang H, Ma RS, Hou JF. 2013. Bone response of
broiler chickens (Gallus gallus domesticus) induced by corticosterone.
Comp Biochem Physiol Part A 164(2): 410–416.
Ma D, Liu Q, Zhang M, Feng J, Li X, Zhou Y, Wang X. 2019. iTRAQbased
quantitative proteomics analysis of the spleen reveals innate
immunity and cell death pathways associated with heat stress in
broilers (Gallus gallus). J Proteom 196: 11–21.
Mohamed AS, Abd El Latif MA, Hussein EA, Toson EM, Saleh M,
Kokoszynski D, Elnesr SS, Mohany M, Al-Rejaie SS, Elwan H.
Efficacy of dietary supplementation with zinc-chromium
mixture, organic selenium, or their combinations on growth performance,
carcass traits, and blood profiles of broilers under heat
stress conditions. Anim 13(15): 2539.
Jain NC. 1993. Essentials of veterinary hematology. Philadelphia (PA):
Lea and Febiger. p. 222–257.
Kim M, Kim Y. 2010. Hypocholesterolemic effects of curcumin via
up-regulation of cholesterol 7a-hydroxylase in rats fed a high fat
diet. Nutr Res Pract 4:191–195.
Kijparkorn S, Angkanaporn K. 2003. Effect of turmeric (Curcuma longa
Linn.) as an antioxidant on immune status and growth performances
of stressed broilers. [M.Sc. thesis]. The Chulalongkorn University,
Faculty of Veterinary Science.
Ma Z, Shayeganpour A, Brocks DR, Lavasanifar A, Samuel J. 2007.
High-performance liquid chromatography analysis of curcumin in
rat plasma: application to pharmacokinetics of polymeric micellar
formulation of curcumin. Biomed Chromatogr 21:546–552.
Mashaly MM, Hendricks GL, Kalama MA, Gehad AE, Abbas AO,
Patterson PH. 2001. Effect of heat stress on production parameters
and immune responses of commercial laying hens. Poult Sci
:889–894. https://doi.org/10.1093/ps/83.6.889
Nabavi SF, Daglia M, Moghaddam AH, Habtemariam S, Nabavi SM.
Curcumin and liver disease: from chemistry to medicine.
Compr Rev Food Sci Food Saf 13:62–77.
Nazar FN, Magnoli AP, Dalcero AM, Marin RH. 2012. Effect of feed
contamination with aflatoxin B1 and administration of exogenous
corticosterone on Japanese quail biochemical and immunological
parameters. Poult Sci 91(1):47–54.
Ohtsu H, Yamazaki M, Abe H, Murakami H, Toyomizu M. 2015. Heat
stress modulates cytokine gene expression in the spleen of broiler
chickens. J Poult Sci 52(4):282–287.
Osho SO, Adeola O. 2020. Chitosan oligosaccharide supplementation
alleviates stress stimulated by in-feed dexamethasone in broiler
chickens. Poult Sci 99(4):2061–2067.
Platel K, Srinivasan K. 2000. Influence of dietary spices and their active
principles on pancreatic digestive enzymes in albino rats. Nahrung
:42–46.
Priyadarsini KI. 1997. Free radical reactions of curcumin in membrane
models. Free Radic Biol Med 23:838–843.
Yadav R, Jee B, Awasthi SK. 2015. Curcumin suppresses the production
of pro-inflammatory cytokine interleukin-18 in lipopolysaccharide
stimulated murine macrophage-like cells. Indian J Clin Biochem
(1):109–112. https://doi.org/10.1007/s12291-014-0452-2
Palizgir MT, Akhtaria M, Mahmoudia M, Mostafaeia S, Rezaiemaneshc
A, Shahram F. 2018. Curcumin reduces the expression of interleukin
b and the production of interleukin 6 and tumor necrosis factor
alpha by M1 macrophages from patients with Behcet’s disease.
Immunopharmacol Immunotoxicol. https://doi.org/10.1080/0892
2018.1474921
Puvadolpirod S, Thaxton JP. 2000a. Model of physiological stress in
chickens: response parameters. Poult Sci 79:363–369.
Puvadolpirod S, Thaxton JP. 2000b. Model of physiological stress in
chickens: 2. Dosimetry of adrenocorticotropin. Poult Sci 79:370–
Rahmani M, Golian A, Kermanshahi H, Bassami MR. 2018. Effects
of curcumin or nanocurcumin on blood biochemical parameters,
intestinal morphology and microbial population of broiler chickens
reared under normal and cold stress conditions. J Appl Poult Sci
(1):200–209. https://doi.org/10.1080/09712119.2017.1284077
Rahmani M, Golian A, Kermanshahi H, Bassami MR. 2017. Effects of
curcumin and nanocurcumin on growth performance, blood gas
indices and ascites mortalities of broiler chickens reared under
normal and cold stress conditions. Ital J Anim Sci 16(3):438–446.
Reddy AC, Lokesh BR. 1996. Effect of curcumin and eugenol on iron-induced
hepatic toxicity in rats. Toxicol 107:39–45.
Surai PF. 2002. Natural antioxidants in avian nutrition and reproduction.
Nottingham: Nottingham University Press.
Surh YJ, Han SS, Keum YS, Seo HJ, Lee SS. 2000. Inhibitory effects
of curcumin and capsaicin on phorbol ester-induced activation of
eukaryotic transcription factors, NF-kappaB and AP-1. Biofactors
:107–112.
Suvanated C, Kijparkorn S, Angkanaporn K. 2003. Effect of turmeric
(Curcuma longa linn.) as an antioxidant on immune status and
growth performances of stressed broilers. [M.Sc. thesis]. The Chulalongkorn
University, Faculty of Veterinary Science, Thailand.
Sandur SK, Pandey MK, Sung B, Ahn KS, Murakami A, Sethi G,
Limtrakul P, Badmaev V, Aggarwal BB. 2007. Curcumin, demethoxycurcumin,
bisdemethoxycurcumin, tetrahydrocurcumin and
turmerones differentially regulate anti-inflammatory and anti-proliferative
responses through a ROS-independent mechanism. Carcino28:
–1773.
Srinivasan K, Sambaiah K. 1991. The effect of spices on cholesterol
alphahydroxylase activity and on serum and hepatic cholesterol
levels in the rat. Int J VitamNutr Res 61:364–369
Virden WS, Kidd MT. 2009. Physiological stress in broilers: ramifications
on nutrient digestibility and responses. J Appl Poult Res
:338–347.
Wang R, Jiao H, Zhao J, Wang X, Lin H. 2016. Glucocorticoids enhance
muscle proteolysis through a myostatin-dependent pathway at the
early stage. PLoS One 11(5): e0156225.
Xu YQ, Xing YY, Wang ZQ, Yan SM, Shi BL. 2018. Pre-protective
effects of dietary chitosan supplementation against oxidative
stress induced by diquat in weaned piglets. Cell Stress Chaperones
(4):703–710.
Zhang J, Hou X, Ahmad H, Zhang H, Zhang L, Wang T. 2014. Assessment
of free radicals scavenging activity of seven natural pigments
and protective effects in AAPH-challenged chicken erythrocytes.
Food Chem 145:57–65.
Zhang Z, Wang H, Jiao R, Peng C, Wong YM, Yeung VS, Huang Y, Chen
ZY. 2009. Choosing hamsters but not rats as a model for studying
plasma cholesterol-lowering activity of functional foods. Mol Nutr
Food Res 53:921–930.
