Use of mesophilic Lactic Acid Bacteria in the manufacture of Feta cheese

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Опубликован: Nov 30, 2017
DOI: https://doi.org/10.12681/jhvms.15081
S. B. KARAGEORGIS (Σ.Β. ΚΑΡΑΓΕΩΡΓΗΣ)
Institute of Food Hygiene of Athens,Ministry of Rural Development and Food
D. K. PAPAGEORGIOU (Δ.Κ. ΠΑΠΑΓΕΩΡΓΙΟΥ)
Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki
A. I. MANTIS (Α.Ι. ΜΑΝΤΗΣ)
Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki
S. A. GEORGAKIS (Σ.Α ΓΕΩΡΓΑΚΗΣ)
Laboratory of Milk Hygiene and Technology, Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki
Аннотация

The use of mesophilic lactic acid bacteria (LAB) in the manufacture of Feta cheese was studied. Five selected mesophilic strains, confirmed as Lactobacillus plantarum (2 strains), Lb. paracasei subsp. paracasein Lb. brevis and Lactococcus lactis subsp. lactis, isolated from ripened Feta and Teleme cheeses, were used in 7 different combinations, alone or in combination with Lb. delbrueckii subsp. bulgaricus and Streptococcus thermophilus (the control combination) for the manufacture of Feta cheese. Each combination of strains was used to prepare four different batches of Feta cheese, keeping all the other production parameters according to the traditional technology. The cheese batches were analyzed for bacteriological, chemical and sensory characteristics. The results showed that the populations of lactobacilli and lactococci increased from the beginning of the cheese manufacture reaching a population of more than 7.0 log10cfu/g. This level was maintained during the whole ripening period (60 days) and during the subsequent 60-day storage period. Only in batches prepared with the control combination F8 {Str. thermophilus I Lb. delbrueckii subsp. bulgaricus), did the population of LAB decline to 6.0 log10cfu/g at the end of the ripening period. Also, except for the yeast population which increased, in all other bacterial groups tested (coliforms, staphylococci, total contaminating bacteria, psychrotrophic bacteria, proteolytic and lipolytic bacteria) populations gradually decreased during the ripening period. Results of the chemical analysis showed a sharp increase in acidity (the cheese pH dropped to ca. 4.5 within 3-4 d) and, whereas the values of other chemical indices (moisture content, fat content and NaCl) were stabilized between the 15* and 30* day of ripening, proteolysis (nitrogen soluble in 12% TCA) and lipolysis (ADV) progressed throughout ripening. The assessment of the overall acceptance by the sensory panel was between "very good" and "excellent" for all cheeses. This suggests that the selected mesophilic starter cultures can be used alone or in combination with the traditional culture {Lb.delbrueckii subsp. bulgaricus I Str. thermophilus) in the production of Feta cheese, as the results of this work indicate that the wild (autochthonous) strains of Lb. plantarum and Lc. lactis subsp. Lactis are well adapted to the environmental conditions that prevail in Feta cheese. Batches prepared using these mesophilic starters received the highest score in the assessment of organoleptic quality of Feta cheese. Very good results were also obtained using the combination of the mesophilic starters Lb. brevis and Lc. lactis subsp. lactis or Lb. paracasei subsp. paracasei and Lc. lactis subsp. lactis alone or in combination with Lb. delbrueckii subsp. bulgaricus and Str. thermophilus.

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Библиографические ссылки
Alichanidis E, Polychroniadou A, Vafopoulou Ν (1981). Teleme cheese from deep-frozen curd. J. Dairy Sci. 64:732-739.
Anifantakis EM (1991a). Traditional Feta cheese. In: Feta and Related Cheese, eds. R. K. Robinson and AY. Tamine. Ellis Horwood limited, England, p. 49-69.
Anifantakis EM (1991b). Greek Cheeses a tradition of centuries, National Dairy Committee of Greece.
APHA (1992). Standard Methods for the Examination of Dairy Products 16th ed., American Public Health Association, Washington.
Cogan TM, Barbosa M, Beuvier E, Bianchisalvadori Β, Cocconcelli PS, Fernandes I, Gomez J, Gomez R, Kalantzopoulos G, Ledda
A, Medina M, Rea MC, Rodriquez E (1997). Characterization of the Lactic-Acid Bacteria in artisanal dairy-products. J. Dairy Res. 64:409-421.
Commission Regulation (EC) 1829/2002 of October 14 2002 amending the Annex to Regulation (EC) No 1107/96 with regard to the name "Feta".
Deeth HS, Fitz-Gerald CH (1976). Lipolysis in dairy products: A Review. Austr. J. Dairy Technol. 31:53-64.
Food and Beverage Code (F.B.C.) (1998). National Press, Athens (in Greek).
Giannakopoulos AL (1996). Analysis of data from biological experiments. Modern Education Publishing company, Thessaloniki (in Greek).
IDF (1988). Yogurt. Enumeration of characteristic microorganisms. Colony count technique at 37° C. Standard No 117A, International Dairy Federation, Brussels, Belgium.
IDF (1991). Butter, fermented milk and fresh cheese. Enumeration of contaminating microorganisms. Colony count technique at 30°C. Standard No 153, International Dairy Federation, Brussels, Belgium.
IDF (1991a). Milk. Enumeration of psychrotrophic microorganisms (colony count technique at 6.5°C). Standard No 101A, International Dairy Federation, Brussels, Belgium.
IDF (1996). Milk and milk products. Preparation of samples and dilutions for microbiological examination. Standard No 122,International Dairy Federation, Brussels, Belgium.
IDF (1997). Milk and milk based products. Enumeration of coagulate positive staphylococci. Colony count technique. Standard No 145A, International Dairy Federation, Brussels, Belgium.
IDF (1998). Milk and milked based products. Enumeration of Coliforms. Part 1. Colony count technique at 30 °C without resuscitation. Part 2. Most Probable Number technique at 30 °C without resuscitation. Standard No 73B, International Dairy Federation, Brussels, Belgium.
Issanchou S, Schlich Ρ, Lesschaeve I (1997). Sensory analysis methodological aspects relevant to the study of cheese. Lait 77:5-12.
Jellinek G (1985). Sensory evaluation of food. Theory and practice, Ellis Horwood Ltd., England and VCH Verlagsgesellchaft mbH, Germany.
Kaminarides SE, Laskos NS (1992). Yeasts in factory brine of Feta cheese. Austr. J. Dairy Technol. 47:68-71.
Karageorgis SB, Papageorgiou DK, Fletouris DI, Mantis AI, Georgakis SA (2006). Isolation, identification and selection of mesophilic lactic acid bacteria from ripened traditional white brined cheese. Greek J. of Dairy Sci. and Technol., (accepted) (in Greek)
Karaioannoglou P, Koidis P, Papageorgiou D, Mantis A (1985).Survival of Yersinia enterocolitica during the manufacture and storage of Feta cheese. Milchwissenschaft 40:204-206.
Katos AB (1986). Biostatistics. Paratiritis publishing company, Thessaloniki (in Greek).
Katsiari MC, Voutsinas LP, (1994). Manufacture of low-fat Feta cheese. Food Chem. 49:53-60.
Law ΒΑ, Castanon M, Shappe E (1976). The effect of non-starter bacteria on chemical composition and flavour of Cheddar cheese. J. Dairy Res. 43:117-125.
Litopoulou-Tzanetaki E, Tzanetakis Ν (1992). Microbiological study of white-brined cheese made from raw goat milk. Food Microbiol. 9:13-19.
Litopoulou-Tzanetaki E, Tzanetakis N, Vafopoulou-Mastrojiannaki A (1993). Effect of the type of lactic starter on microbiological chemical and sensory characteristics of Feta cheese. Food Microbiol. 10:31-41.
Lucey JA, Fox PF (1993). Importance of calcium and phosphate in cheese manufacture - A review. J. Dairy Sci. 76:1714-1724.
Mallatou H, Pappas CP, Voutsinas LP (1994). Manufacture of Feta cheese from sheep's milk, goat's milk or mixtures of these milks. Int. Dairy J. 4:641-664.
Manolkidis C, Polychroniadou A, Alichanidis E (1970a). Variation dans la composition du fromage Teleme au cours de sa maturation. Lait 50:38-48.
Manolkidis C, Polychroniadou A, Alichanidis E (1970b). Study of proteolysis during ripening of Teleme cheese. Lait 50:128-135.
Mantis AJ (1973). Production of staphylococcal enterotoxins in white-brined cheese feta. Docent-Doctoral Thesis. School of Vet. Med. Univ. Thessaloniki, (in Greek).
Mantis AJ (2000). Hygiene and technology on milk and milk products. Kyriakides brothers publishing company, Thessaloniki (in Greek).
Mantis AJ, Karaioannoglou Ρ (1999). Laboratory Food Microbiology, 2nd ed., Thessaloniki (in Greek).
Melas DS, Papageorgiou DK, Abrahim A, Mantis A (2001). Survival oîAeromonas hydrophila during the manufacture and ripening of Feta cheese. Milchwissenshaft. 56: 257-261.
Panetsos A, Georgakis SA, Mantis AJ, Karaioannoglou Ρ (1971). Survival of Brucella melitensis in experimentally contaminated Feta cheese. Proceedings, 4th National Symposium of Microbiology, Athens, pp. 171-179 (in Greek).
Papageorgiou DK, Abrahim A, Bori M, Doudounakis S (1998). Chemical and bacteriological characteristics of Pichtogalo Chanion cheese and mesophilic starter cultures for its production. J. Food Prot. 61:688-692.
Papageorgiou DK, Marth EH (1989). Fate of Listeria monocytogenes during the manufacture, ripening and storage of Feta cheese. J. Food Protect. 52:82-87.
Papageorgiou DK (2004). Application of Hazard Analysis Critical Control Points (HACCP) in the production line of Feta cheese. Proceedings, 3rd Hellenic Symposium on food hygiene and food technology, Athens. Vol. B, pp. 32-39 (in Greek).
Pappas CP, Kondyli E, Voutsinas LP, Mallatou H (1994). Effect of standardization of ewe's milk for casein/fat ration on the composition, sensory and rheological properties of Feta cheese. Int. Dairy J. 4:763-778.
Pappas CP, Kondyli E, Voutsinas LP, Mallatou H (1996a). Effects of starter level, draining time and aging on the physicochemical, organoleptic and rheological properties of Feta cheese. J. Soc.
Dairy Technol. 49:73-78.
Pappas CP, Kondyli E, Voutsinas LP, Mallatou H (1996b). Effects of salting method and storage time on composition and quality of Feta cheese. J. Soc. Dairy Technol. 49:113-118.
Peterson SD, Marshall RT (1990). Non-starter Lactobacilli in Cheddar cheese: A review. J. Dairy Sci. 73:1395-1410.
Polychroniadou A, Vlachos J (1979). The free amino acids of Teleme cheese. Lait 59:234-243.
Scott R (1986). Cheesemaking practice, second ed. Elsevier Appi. Sci. pubi.
Spinnler HE, Corrieu G (1989). Automatic method to quantify starter activity based on pH measurement. J. Dairy Res. 56:755-764.
Tukan SK, Humeid MA (1991). Salt and moisture relationships in brined cheese. Austr. J. Dairy Technol. 46:85-87.
Tzanetakis N, Litopoulou-Tzanetaki E (1988). Lactic acid bacteria in raw goat milk and some of their biochemical properties. Microbiol. Aliments Nutr. 7:73-80.
Tzanetakis N, Litopoulou-Tzanetaki E (1992). Changes in numbersand kinds of Lactic-Acid Bacteria in Feta and Teleme. 2 Greek cheeses from ewes milk. J. Dairy Sci. 75:1389-1393.
Tzanetakis N, Vafopoulou-Mastrojiannaki A, Litopoulou-Tzanetaki E (1995). The quality of white-brined cheese from goats milk made with different starters. Food Microbiol. 12:55-63.
Vafopoulou A, Zerfiridis G, Alichanidis E (1989). Accelerated ripening of Feta cheese, with heat-shocked cultures or microbial proteinases. J. Dairy Res. 56:285-296.
Vakaleris DG, Price WV (1959). A rapid spectrophotometric method for measuring cheese ripening. J. Dairy Sci. 42:264-276.
Vivier D, Rivemale M, Reverbel JP, Ratomahenina R, Galzy Ρ (1994). Study of the growth of yeasts from feta cheese. Int. J. Food Microbiol. 22:207-215.
Westall S, Filtenborg Ο (1998). Yeast occurrence in Danish Feta cheese. Food Microbiol. 15:215-222.
Yanai Y, Rosen B, Pinoky A, Sklan D (1977). The microbiology of pickled cheese during manufacture and maturation. J. Dairy Res. 44:149-153.
Zourari A, Desmazeaud MJ (1991). Characterization of Lactic Acid Bacteria isolated from Greek yogurts. 2. Strains of Lactobacillus delbrueckii subsp. bulgaricus and mixed cultures with Streptococcus salivarius subsp thermophilus. Lait 71:463-482.
Zourari A, Roger S, Chabanet C, Desmazeaud MJ (1991). Characterization of Lactic Acid Bacteria isolated from Greek yogurts. 1. Streptococcus salivarius subsp. thermophilus strains.Lait 71:445-461.
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