A study of biochemical changes in river buffaloes (Bubalus bubalis) naturally infected with Anaplasma marginale


Опубликован: Jul 4, 2023
Версии:
2023-07-04 (1)
SM Jalali
https://orcid.org/0000-0003-0188-5974
AA Nikvand
https://orcid.org/0000-0002-4837-6766
D Gharibi
https://orcid.org/0000-0002-3614-0254
M Razi Jalali
https://orcid.org/0000-0003-2092-5652
M Yazdkhasti
https://orcid.org/0000-0001-7288-8801
F Kaviani
https://orcid.org/0000-0001-6835-7725
Аннотация

Anaplasma marginale as an intracellular rickettsia, endemic in tropical and subtropical regions of the world. This study was performed to determine A. marginale infection, molecularly, and to investigate its effects on some serum biochemical parameters in river buffaloes referred to Ahvaz abattoir, Southwest Iran. A total of 103 apparently healthy river buffaloes were randomly sampled. Whole blood samples were subjected to PCR analysis and blood smears were examined microscopically for the presence of Anaplasma inclusions. Serum biochemical parameters including total protein, albumin, glucose, urea, iron, total bilirubin, total cholesterol, Calcium, Phosphorus and Magnesium concentrations, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities and also serum electrolytes comprising Na and K were assessed. The results revealed that 16 and 32 samples were infected with A. marginale in microscopic and PCR assessment, respectively. Serum biochemical analysis showed no significant difference between A. marginale infected and non-infected buffalo groups. It can be concluded that infection with A. marginale does not lead to much pathogenicity in river buffaloes, so that in infected animals, no clinical signs or abnormalities in the performance indicators of various organs can be detected at the laboratory level. However, the role of this species as a reservoir of this microorganism in nature and in the epidemiology and transmission of anaplasmosis to other species remains significant. Buffaloes can be a substitute to raising cattle in regions with high occurrence of anaplasmosis.

Article Details
  • Раздел
  • Research Articles
Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Allen PC, Kuttler KL, Amerault TE (1981) Clinical chemistry of anaplasmosis: blood chemical changes in infected mature cows. Am J Vet Res 42(2): 322-325.
Amira AH, Răileanu C, Tauchmann O, Fischer S, Nijhof AM, Silaghi C (2020) Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt. Parasites Vectors 13(1): 1-11.
Bacanelli GM, Ramos CAN, Araújo FR (2014) Molecular diagnosis of Anaplasma marginale in cattle: quantitative evaluation of a real-time PCR (Polymerase Chain Reaction) based on msp5 gene. Pesq Vet Bras 34(1):29-33. http://dx.doi.org/10.1590/S0100-736X2014000100005
Benitez D, Mesplet M, Echaide I, Torioni de Echaide S, Schnittger L, Florin-Christensen M (2018) Mitigated clinical disease in water buffaloes experimentally infected with Babesia bovis. Ticks Tick Borne Dis 9(5):1358-1363. <http://dx.doi.org/10.1016/j.ttbdis.2018.04.012>
Constable PD, Hinchcliff KW, Done SH, Grunberg W (2017) Anaplasmosis. In: Veterinary Medicine, 11th ed., Elsevier, China P. 769-775.
Coskun A, Ekici O D, Guzelbektes H, Aydogdu U, Sen I (2012) Acute phase proteins, clinical, hematological and biochemical parameters in dairy cows naturally infected with Anaplasma marginale. Kafkas Univ Vet Fak Derg 18(3): 497-502.
Ganguly A, Maharana BR, Ganguly INDRAJIT, Kumar ANKIT, Potlya S, Arora D, Bisla RS (2018) Molecular diagnosis and haemato-biochemical changes in Anaplasma marginale infected dairy cattle. Indian J Anim Sci 88(9): 989-993.
Guatam OP, Sharma RD, Singh B (1970) Anaplasmosis-II. Clinical cases of anaplasmosis in cattle, buffaloes and sheep. Indian Vet J 47:1012-1019.
Jassem GA, Agaar OA (2015) Molecular and biochemical study of Anaplasma marginale in cattle in Wassit Province of Iraq. African J Bacteriol Res 7(4): 36-41.
Jones EW, Brock WE (1966) Bovine anaplasmosis: its diagnosis, treatment, and control. JAVMA 149:1624–1633.
Kocan KM, de la Fuente J, Blouin EF, Coetzee JF, Ewing SA (2010) The natural history of Anaplasma marginale. Vet Parasitol 167(2-4): 95-107.
Kocan KM, de la Fuente J, Guglielmone AA, Melendez RD (2003) Antigens and alternatives for control of Anaplasma marginale infection in cattle. Clin Microbiol Rev 16:698–712.
Kocan KM, de la Fuente J, Blouin EF, Garcia-Garcia JC (2004) Anaplasma marginale (Rickettsiales: Anaplasmataceae): recent advances in defining host-pathogen adaptations of a tick-borne rickettsia. Parasitol 129: S285–S300.
Kuttler KL (1984) Anaplasma infections in wild and domestic ruminants: a review. J Wildlife Dis 20: 12–20.
Lima DH, Vinhote W, Ubiali DG, Soares PC, Cordeiro MD, Silva JB, Fonseca AH, Barbosa JD (2019) Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects. Pesq Vet Bras 39(9): 700-709.
Mamun MAA, Begum N, Bari MA, Mondal MMH (2010) Haemoprotozoa of Buffaloea (Bubalus bubalis) in Kurigram. Bangladesh J Prog Sci Tech 8: 209-212.
Nikvand AA, Hasanpour Besati E, Gharibi D, Jalali SM (2020) Molecular and Hematologic Survey on Anaplasma marginale Infection in Slaughtered Water Buffaloes (Bubalous bubalis) in Ahvaz City, Iran. J Vet Res 75(2): 192-199.
Obregón D, Corona BG, de la Fuente J, Cabezas-Cruz A, Gonçalves LR, Matos CA, Armas Y, Hinojosa Y, Alfonso P, Oliveira MC, Machado RZ (2018) Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba. Vet Parasitol: Reg Stud 13: 180-187.
Reddy GR, More T, Sharma SP, Singh LN (1988) The oxidant defense system in water-buffaloes (Bubalus bubalis) experimentally infected with Anaplasma marginale. Vet Parasitol 27(3/4):245-249. <http://dx.doi. org/10.1016/0304-4017(88)90039-8>
Rymaszewska A, Grenda S (2008) Bacteria of the genus Anaplasma–characteristics of Anaplasma and their vectors: a review. Vet Med 53(11): 573-584.
Saetiew N, Simking P, Inpankaew T, Wongpanit K, Kamyingkird K, Wongnakphet S, Stich RW, Jittapalapong S (2015) Prevalence and genetic diversity of Anaplasma marginale infections in water buffaloes in Northeast Thailand. J Trop Med Parasitol 38: 9-16.‏
Sarangi LN, Rana SK, Prasad A, Ponnanna NM, Sharma GK (2020) Prevalence of antibodies to Anaplasma in cattle and buffaloes of different organized herds in India. J Parasit Dis 12:1-7.
Sharma SP (1987) Characterization of Anaplasma marginale infection in buffaloes. Indian J Anim Sci 57:76-78.
Sharma A, Singla LD, Kaur P, Bal MS, Batth BK, Juyal PD (2013) Prevalence and haemato-biochemical profile of Anaplasma marginale infection in dairy animals of Punjab (India). Asian Pacific J Trop Med 6(2): 139-144.
Silva JB, Fonseca AH, Barbosa JD, Cabezas-Cruz A, de la Fuente J (2014a). Low genetic diversity associated with low prevalence of Anaplasma marginale in water buffaloes in Marajó Island, Brazil. Ticks Tick Borne Dis 5: 801-804.‏
Silva JB, Vinhote WMS, Oliveira CMC, André MR, Machado RZ, da Fonseca AH, Barbosa JD (2014b) Molecular and serological prevalence of Anaplasma marginale in water buffaloes in northern Brazil. Ticks Tick Borne Dis 5(2): 100-104.
Singh A, Gill BS (1977) Note on the prevalence of subclinical anaplasmosis (Anaplasma marginale) in three herds of cattle and buffaloes in Punjab State. Indian J Anim Sci 47: 224-226.
Sisson D, Hufschmid J, Jolles A, Beechler B, Jabbar A (2017) Molecular characterisation of Anaplasma species from African buffalo (syncerus caffer) in kruger national park, South Africa. Ticks Tick Borne Dis 8: 400-406.
Srivastava R, Ahluwalia SS (1974) A clinical case of anaplasmosis in buffalo. Indian Vet J 51:371-374.
Vatsya S, Kumar RR, Singh VS, Arunraj MR (2013) Anaplasma marginale infection in a buffalo: a case report. Vet Res Int 1(2):51-53.
Ybanez AP, Sivakumar T, Ybanez RHD, Ratilla JC, Perez ZO, Gabotero SR, Inokuma H (2013) First molecular characterization of Anaplasma marginale in cattle and Rhipicephalus (Boophilus) microplus ticks in Cebu, Philippines. J Vet Med Sci 75, 27-36.‏
Наиболее читаемые статьи этого автора (авторов)