Microbiological evaluation of ready-to-eat foods by conventional methods and MALDI-TOF MS

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DOI: https://doi.org/10.12681/jhvms.27518
P. KAYNAR MURSALOĞLU
Hitit Üniversity, Alaca Avni Çelik Vocational School, Çorum-Turkey
Y. NUMANOĞLU ÇEVIK
Microbiology and Reference Laboratory and Biological Products Department, General Directorate of Public Health, Minister of Health, Ankara-Turkey
Аннотация

The consumption of ready-to-eat foods in daily life is increasing rapidly. Food poisoning occurs as a result of the preparation and preservation of these foods under inappropriate hygienic conditions. There is also an increased risk of getting food poisoning during the summer heat. The purpose of this study was to evaluate the microbiological properties of ready-to-eat foods by conventional methods and investigate the applicability of using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of microorganisms in the bacteria isolates from these foods. Eighteen ready-to-eat food samples (tomato soup-G1, lentil soup-G2, tarhana soup-G3, yogurt soup-G4, ezo gelin soup-G5, chicken stew-G6, eggplant ragout-G7, green beans with meat-G8, bulgur pilaf-G9, rice pilaf-G10, macaroni-G11, rice pudding-G12, raspberry fruit dessert-G13, tomato salad G14, cucumber salad-G15, lettuce-G16, shepherd’s salad-G17, and yogurt-G18) on sale in restaurants in Ankara-Turkey were evaluated. For this evaluation, the samples were analyzed in terms of staphylococcal enterotoxin (SET-RPLA), Bacillus cereus (EN ISO 7932), Salmonella spp. (EN ISO 6579-1), Listeria monocytogenes (EN ISO 11290-1) and Escherichia coli (ISO 16649-2) using conventional microbiological methods. The use of MALDI-TOF MS for the identification of bacteria isolates was also evaluated in this study. Only bulgur pilaf-G9, raspberry fruit dessert-G13, and lettuce-G16 samples exhibited microbial growth on agar plates. Klebsiella pneumoniae was detected following the identification of the suspected Salmonella spp. in the bulgur pilaf-G9 sample. B. cereus (50 cfu /g) and E. coli (80 cfu /g) were detected in raspberry fruit dessert-G13 and lettuce-G16, respectively. These bacteria, which were isolated and identified by conventional methods were also rapidly confirmed by MALDI-TOF. In conclusion, these sampled foods, which were available for public consumption, met the general hygiene criteria. Therefore, the foods complied with the Turkish national legislation. The MALDI-TOF MS method has advantages over conventional methods employed in the microbiological evaluation of these foods, in terms of shorter application time, rapidity, and greater simplicity in identifying the causes of poisoning and causative agents involved in food poisoning.

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