Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows
Аннотация
The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.
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El Zeer, A., Fouda, M., Darwish, R., & Ateya, A. (2023). Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows. Journal of the Hellenic Veterinary Medical Society, 73(4), 5021–5030. https://doi.org/10.12681/jhvms.29524
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- Том 73 № 4 (2022)
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- Research Articles
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Библиографические ссылки
Aida, Y (1995) Characterization and expression of bovine MHC class II
genes. Bulletin de la Societe Franco-Japonaise Des Sciences Veterinaires 6: 17-24.
Al-Waith, HK, Mohamed, TR,Al-Anbari, NN (2018) Association between
DQA1 gene polymorphism and reproductive, immunity performance
and heat tolerance in Holstein cattle. Plant Archives 18: 2681-2686.
Altschul, SF, Gish, W, Miller, W, Myers, EW,Lipman, DJ (1990) Basic
local alignment search tool. Journal of Molecular Biology 215, (3):
-410.
Amills, M, Ramiya, V, Norimine, J,Lewin, HA (1998) The major histocompatibility complex of ruminants. Scientific & Technical Review
, (1): 108-120.
Andersson, L, Bóhme, J, Peterson, PA,Rask, L (1986) Genomic hybridization of bovine class II major histocompatibility genes: 2. Polymorphism of DR genes and linkage disequilibrium in the DQ-DR region.
, (2): 295-304.
Anton, I, Kovács, K, Holló, G, Farkas, V, Szabó, F, Egerszegi, I, Rátky,
J, Zsolnai, A,Brüssow, KP (2012) Effect of DGAT1, leptin, and TG
gene polymorphisms on some milk production traits in different dairy
cattle breeds in Hungary. Arch. Anim. Breed. 55, (4): 307-314.
Ardicli, S, Samli, H, Dincel, D, EKIZ, B, YALCINTAN, H, Vatansever,
B,Balci, F (2018) Relationship of the bovine IGF1, TG, DGAT1 and
MYF5 genes to meat colour, tenderness and cooking loss. Journal of
the Hellenic Veterinary Medical Society 69, (3): 1077-1087.
Band, M, Larson, JH, Womack, JE,Lewin, HA (1998) A Radiation Hybrid Map of BTA23: Identification of a Chromosomal Rearrangement
Leading to Separation of the Cattle MHC Class II Subregions. Genomics 53, (3): 269-275.
Boesenberg-Smith, KA, Pessarakli, MM,Wolk, DM (2012) Assessment of
DNA Yield and Purity: an Overlooked Detail of PCR Troubleshooting. Clinical Microbiology Newsletter 34, (1): 1-6.
Boldt, ABW, Beltrame, MH, Catarino, SJ, Meissner, CG, Tizzot, R,Messias-Reason, IJ (2016) A dual role for Mannan-binding lectin-associated serine protease 2 (MASP2) in HIV infection. Molecular Immunology 78: 48-56.
Boom, R, Sol, CJ, Salimans, MM, Jansen, CL, Dillen, PMW-v,Noordaa,
Jvd (1990) Rapid and simple method for purification of nucleic acids.
Journal of Clinical Microbiology 28, (3): 495-503.
Bradley, LM, Dalton, DK,Croft, M (1996) A direct role for IFN-gamma
in regulation of Th1 cell development. The Journal of Immunology
, (4): 1350-1358.
Brym, P, Kamiński, S,Wójcik, E (2005) Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations
with milk performance traits. Journal of applied genetics 46, (2): 179-
Buchanan, FC, Van Kessel, AG, Waldner, C, Christensen, DA, Laarveld,
B,Schmutz, SM (2003) Hot Topic: An Association Between a Leptin
Single Nucleotide Polymorphism and Milk and Protein Yield. Journal
of Dairy Science 86, (10): 3164-3166.
Bulla, J, Polák, P,Chrenek, P (2013) Pinzgauer cattle in Slovakia. Slovak
Journal of Animal Science 46, (4): 151-154.
Carvalho, TDd, Siqueira, F, Torres Júnior, RAdA, Medeiros, SRd, Feijó,
GLD, Souza Junior, MDd, Blecha, IMZ,Soares, CO (2012) Association of polymorphisms in the leptin and thyroglobulin genes with
meat quality and carcass traits in beef cattle. Revista Brasileira de
Zootecnia 41: 2162-2168.
Chen, M, Liang, Y, Li, W, Wang, M, Hu, L, Abuaku, BK, Huang, X, Tan,
H,Wen, SW (2015) Impact of MBL and MASP2 gene polymorphism
and its interaction on susceptibility to tuberculosis. BMC Infectious
Diseases 15, (1): 151.
Curi, RA, Palmieri, DA, Suguisawa, L, Ferraz, ALJ, de Oliveira, HN, Furlan, LR, Silveira, AC,Lopes, CR (2006) Effects of GHR gene polymorphisms on growth and carcass traits in Zebu and crossbred beef
cattle. Livestock Science 101, (1): 94-100.
Davies, CJ, Andersson, L, Mikko, S, Ellis, SA, Hensen, EJ, Lewin, HA,
Muggli-Cockett, NE, Poel, JJvd,Russell, GC (1997) Nomenclature
for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee. Animal Genetics 28, (3): 159-168.
Ding, W, Qiu, Q, Liu, G, Liu, J, Mao, R,Lin, B (2014) Metadata checklist:
identification of CHI3L1 and MASP2 as a biomarker pair for liver cancer through integrative secretome and transcriptome analysis.
Omics : a journal of integrative biology 18, (10): 658-661.
Dobicki, A, Walawski, K, Chladek, G, Nowopolska, A, Koliski, W,Zachwieja, A (2002) Dairy traits of the progeny of Red-and-White bulls
described through the genetic polymorphism of milk proteins and
principally of fraction alphaS1-CN. Annals of Animal Science 2, (1).
Dolmatova, I, Sedykh, T, Valitov, F, Gizatullin, R, Khaziev, D,Kharlamov,
A (2020) Effect of the bovine TG5 gene polymorphism on milk- and
meat-producing ability. Veterinary world 13, (10): 2046-2052.
FAO (2011) Molecular genetic characterization of animal genetic resources, Food and Agriculture Organization Animal Production Health
Guidelines Rome, Italy. No. 9.
Falconer, D.S. and Mackay, T.F.C. (1996). Introduction to Quantitative
Genetics. 4th Edn., Benjamin Cummings, London, UK., ISBN-13:
: 464.
Gan, Q-F, Zhang, L-P, Li, J-Y, Hou, G-Y, Li, H-D, Gao, X, Ren, H-Y,
Chen, J-B,Xu, S-Z (2008) Association analysis of thyroglobulin gene
variants with carcass and meat quality traits in beef cattle. Journal of
Applied Genetics 49, (3): 251-255.
Gautier, M, Faraut, T, Moazami-Goudarzi, K, Navratil, V, Foglio, M,
Grohs, Cc, Boland, A, Garnier, J-G, Boichard, D, Lathrop, GM, Gut,
IG,Eggen, A (2007) Genetic and Haplotypic Structure in 14 European
and African Cattle Breeds. Genetics 177, (2): 1059-1070.
Ghasemi, N, Zadehrahmani, M, Rahimi, G,Hafezian, SH (2009) Associations between prolactin gene polymorphism and milk production in
montebeliard cows. International Journal of Genetics Molecular Biology and Evolution 1, (3): 048-051.
Glass, EJ, Oliver, RA,Russell, GC (2000) Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle. 165,
(1): 134-138.
Goeldner, I, Skare, T, Boldt, ABW, Nass, FR, Messias-Reason, IJ,Utiyama, SR (2014) Association of MASP2 Levels and MASP2 Gene Polymorphisms with Rheumatoid Arthritis in Patients and Their Relatives.
PLOS ONE 9, (3): e90979.
Groeneveld, LF, Lenstra, JA, Eding, H, Toro, MA, Scherf, B, Pilling, D,
Negrini, R, Finlay, EK, Jianlin, H, Groeneveld, E, Weigend, S,Consortium, TG (2010) Genetic diversity in farm animals - a review. Animal Genetics 41, (s1): 6-31.
Gutiérrez, JP, Altarriba, J, Díaz, C, Quintanilla, R, Cañón, J,Evol., JPJGS
(2003) Pedigree analysis of eight Spanish beef cattle breeds. 35, (1):
-63.
Holm, S (1979) A Simple Sequentially Rejective Multiple Test Procedure.
Scandinavian Journal of Statistics 6, (2): 65-70.
Ibeagha-Awemu, EM, Prinzenberg, EM, Jann, OC, Lühken, G, Ibeagha,
AE, Zhao, X,Erhardt, G (2007) Molecular Characterization of Bovine
CSN1S2*B and Extensive Distribution of Zebu-Specific Milk Protein
Alleles in European Cattle. Journal of Dairy Science 90, (7): 3522-
Kamiński, S,Zabolewicz, T (2000) Associations between bovine beta-lactoglobulin polymorphism within coding and regulatory sequences and
milk performance traits. Journal of Applied Genetics 41, (2): 91-99.
Karimi, K, Beigi Nassiri, MT, Mirzadeh, K, Ashayerizadeh, A, Roushanfekr, H,Fayyazi, J (2009) Polymorphism of the b-Lactoglobulin Gene
and Its Association with Milk Production Traits in Iranian Najdi Cattle. Iranian Journal of Biotechnology 7, (2): 82-85.
Kasprzak-Filipek, K, Sawicka-Zugaj, W, Litwińczuk, Z, Chabuz, W,
Šveistienė, R,Bulla, J (2019) Assessment of the genetic structure of
Central European cattle breeds based on functional gene polymorphism. Global Ecology and Conservation 17: e00525.
Khatkar, MS, Thomson, PC, Tammen, I,Raadsma, HW (2004) Quantitative trait loci mapping in dairy cattle: review and meta-analysis.
Genetics Selection Evolution 36, (2): 163.
Komisarek, J,Antkowiak, I (2007) The relationship between leptin gene
polymorphisms and reproductive traits in Jersey cows. Polish journal
of veterinary sciences 10, (4): 193-197.
Liefers, SC, te Pas, MFW, Veerkamp, RF,van der Lende, T (2002) Associations between Leptin Gene Polymorphisms and Production, Live
Weight, Energy Balance, Feed Intake, and Fertility in Holstein Heifers. Journal of Dairy Science 85, (6): 1633-1638.
MacHugh, DE, Shriver, MD, Loftus, RT, Cunningham, P,Bradley, DG
(1997) Microsatellite DNA Variation and the Evolution, Domestication and Phylogeography of Taurine and Zebu Cattle (Bos taurus and
Bos indicus). Genetics 146, (3): 1071-1086.
McKay, SD, Schnabel, RD, Murdoch, BM, Matukumalli, LK, Aerts, J,
Coppieters, W, Crews, D, Neto, ED, Gill, CA, Gao, C, Mannen, H,
Wang, Z, Van Tassell, CP, Williams, JL, Taylor, JF,Moore, SS (2008)
An assessment of population structure in eight breeds of cattle using
a whole genome SNP panel. BMC Genetics 9, (1): 37.
Petrakova, L, Kerzienė, S,Razmaitė, V (2012) Contribution of different
breeds to Lithuanian Red cattle using pedigree information with only
a fraction of the population analyzed. Veterinarija ir Zootechnika 57,
(79): 62-66.
Rahbar, R, Rahimi, G, Pirsaraei, ZA,Gholizadeh, M (2010) Identification
of polymorphism in promoter region of growth hormone receptor
(GHR) gene and its association with milk related traits in Holstein
cows. African Journal of Biotechnology 9, (33).
Russell, GC, Davies, CJ, Andersson, L, Mikko, S, Ellis, SA, Hensen, EJ,
Lewin, HA, Muggli-Cockett, NE,Poel, JJvd (1997) BoLA class II nucleotide sequences, 1996: report of the ISAG BoLA Nomenclature
Committee. Animal Genetics 28, (3): 169-180.
Sanger, F, Nicklen, S,Coulson, AR (1977) DNA sequencing with
chain-terminating inhibitors. Proceedings of the National Academy
of Sciences 74, (12): 5463-5467.
Schennink, A, Bovenhuis, H, Léon-Kloosterziel, KM, Van Arendonk,
JAM,Visker, MHPW (2009) Effect of polymorphisms in the FASN,
OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition. Animal Genetics 40, (6): 909-916.
Selionova, MI, Dubovskova, MP, Chizhova, LN, Mikhailenko, AK, Surzhikova, ES,Plakhtyukova, VR (2019) Fatty acid composition of
blood lipids of young beef cattle of different genotypes of CAPN1,
GH, TG5, LEP genes. IOP Conference Series: Earth and Environmental Science 341, (1): 012079.
Signorelli, F, Orrù, L, Napolitano, F, De Matteis, G, Scatà, MC, Catillo, G,
Marchitelli, C,Moioli, B (2009) Exploring polymorphisms and effects
on milk traits of the DGAT1, SCD1 and GHR genes in four cattle
breeds. Livestock Science 125, (1): 74-79.
Sigurdardóttir, S, Borsch, C, Gustafsson, K,Andersson, L (1992) Exon
encoding the antigen-binding site of MHC class II beta-chains is divided into two subregions with different evolutionary histories. The
Journal of Immunology 148, (3): 968-973.
Silva, AA, Catarino, SJ, Boldt, ABW, Pedroso, MLA, Beltrame, MH,Messias-Reason, IJ (2018) Effects of MASP2 haplotypes and MASP2 levels in hepatitis C-infected patients. International Journal of Immunogenetics 45, (3): 118-127.
Singh, U, Deb, R, Alyethodi, RR, Alex, R, Kumar, S, Chakraborty, S,
Dhama, K,Sharma, A (2014) Molecular markers and their applications in cattle genetic research: A review. Biomarkers and Genomic
Medicine 6, (2): 49-58.
Socol, CT, Iacob, L, Mihalca, I,Criste, FL (2015): Molecular and population genetics tools for farm animal genetic resources conservation:
brief overview. Scientific Papers Animal Science and Biotechnologies.
Spelman, RJ,Bovenhuis, H (1998) Moving from QTL experimental results to the utilization of QTL in breeding programmes. Animal Genetics 29, (2): 77-84.
Svensson, EM, Anderung, C, Baubliene, J, Persson, P, Malmström, H,
Smith, C, Vretemark, M, Daugnora, L,Götherström, A (2007) Tracing
genetic change over time using nuclear SNPs in ancient and modern
cattle. Animal Genetics 38, (4): 378-383.
Świerzko, AS, Szala-Poździej, A, Kilpatrick, DC, Sobociński, M, Chojnacka, K, Sokołowska, A, Michalski, M, Mazerant, K, Jensenius,
JC, Matsushita, M, Krajewski, WR, Szczapa, J, Bąk-Romaniszyn, L,
Zeman, K,Cedzyński, M (2016) Components of the lectin pathway of
complement activation in paediatric patients of intensive care units.
Immunobiology 221, (5): 657-669.
Taberlet, P, Valentini, A, Rezaei, HR, Naderi, S, Pompanon, F, Negrini,
R,Ajmone-Marsan, P (2008) Are cattle, sheep, and goats endangered
species? Molecular Ecology 17, (1): 275-284.
Takeshima, S-N,Aida, Y (2006) Structure, function and disease susceptibility of the bovine major histocompatibility complex. Animal Science Journal 77, (2): 138-150.
Tamura, K, Dudley, J, Nei, M,Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution 24, (8): 1596-1599.
Troy, CS, MacHugh, DE, Bailey, JF, Magee, DA, Loftus, RT, Cunningham, P, Chamberlain, AT, Sykes, BC,Bradley, DG (2001) Genetic
evidence for Near-Eastern origins of European cattle. Nature 410,
(6832): 1088-1091.
Tsakanova, G, Stepanyan, A, Nahapetyan, K, Sim, RB, Arakelyan, A,Boyajyan, A (2018) Serine proteases of the complement lectin pathway
and their genetic variations in ischaemic stroke. Journal of Clinical
Pathology 71, (2): 141-147.
Tyul’kin, SV, Akhmetov, TM, Valiullina, EF,Vafin, RR (2013) Polymorphism of somatotropin, prolactin, leptin, and thyreoglobulin genes in
bulls. Russian Journal of Genetics: Applied Research 3, (3): 222-224.
Vandre, R, Sharma, A, Gowane, G, Ravi, R, Shweta, R, Sinha, R, Amod,
K, Madhu, S, Caser, D,Meshram, S (2014) Polymorphism and disease
resistance possessions of MHCclass II BoLA genes. Double Helix Research-International Journal of Biomedical and Life Sciences 5, (2):
-372.
Wu, J, Bai, JY, Li, L, Huang, S, Li, CM,Wang, GL (2015) Genetic polymorphisms of the BMAP-28 and MASP2 genes and their correlation
with the somatic cell score in Chinese Holstein cattle. Genet Mol Res
, (1): 1-8.
Xiong, S, Zhao, N, Qiu, Y, Zhang, L, Zuo, D,Chen, Z (2015) Plasma levels of mannan-binding lectin-associated serine protease 2 in children
with upper respiratory tract infection. Journal of Southern Medical
University 35, (6): 888-893.
Ytting, H, Christensen, IJ, Steffensen, R, Alsner, J, Thiel, S, Jensenius, JC,
Hansen, U,Nielsen, HJ (2011) Mannan-Binding Lectin (MBL) and
MBL-Associated Serine Protease 2 MASP2 Genotypes in Colorectal
Cancer. Scandinavian Journal of Immunology 73, (2): 122-127.
Zhang, H, Wei, Y, Zhang, F, Liu, Y, Li, Y, Li, G, Han, B, Wang, H, Zhao,
W,Wang, C (2019) Polymorphisms of MASP2 gene and its relationship with mastitis and milk production in Chinese Holstein cattle.
Biotechnology & Biotechnological Equipment 33, (1): 589-596.
Zinnatova, F,Zinnatov, F (2014) The role of lipid metabolism genes
(DGAT1, TG5) in improving the economically beneficial traits of cattle. Uchenye zapiski Kazanskoi gosudarstvennoi akademii veterinarnoi meditsiny im. NE Baumana 219, (3): 164-168
