Identification of single nucleotide variant in Mx1 gene associated with antibody response to Avian Influenza virus in Aseel chickens SNP in Mx1-gene for antibody response to AIV in Aseel


Опубликован: Oct 31, 2023
Обновлен: 2023-10-31
Версии:
2023-10-31 (2)
R Majeed
I Zahoor
A Anjum
M Ali
A Basheer
Аннотация

Aseel birds are known for their hardiness, thermotolerance, pugnacity, and robustness. However, they are highly susceptible to Avian Influenza (AI) and Newcastle Disease (ND) viruses which cause huge mortalities. Present study was designed to identify polymorphisms in exon-14 of Mx1, and 5’UTR & intron-7 of ROBO2 gene associated with antibody response to AIV (H9N2) and ND virus respectively in adult Aseel birds. After screening of 500 birds for HI-based antibody response against both viruses, two separate set of 40 birds, one for each virus, were selected on the basis of maximum divergence in their antibody response. The sequence data of exon-14 of Mx1 gene revealed 3 SNP, and that of ROBO2 gene showed 2 SNPs in intron-7 and 1 in 5’UTR. The genotypic frequencies of identified variants were tested for goodness-of-fit and only Mx_810 showed significant difference. The results of association analysis revealed a non-synonymous SNP (G>A), Mx1_567, significantly associated with pre- (P<0.01) and post-vaccination (P<0.01) antibody response against AIV. The LSD results showed that homozygous mutant (AA), and heterozygous (AG) genotypes had significantly greater values of pre- and post-vaccination antibody response compared with wild-type genotype. However, no SNP in the ROBO2 gene was found significantly associated with antibody response.

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Биография автора
I Zahoor, University of Veterinary and Animal Sciences, Lahore, Pakistan

Associate Professor

Department of Animal Breeidng and Genetics, University of Veterinary and Animal Sciences, Lahore, Pakistan

Библиографические ссылки
Al-Habib MF, Murtini S, Gunawan A, Ulupi N, Sumantri C. 2020 Poly
morphism of CD1B Gene and Its Association with Yolk Immuno
globulin (IgY) Concentration and Newcastle Disease Antibody Titer
in IPB-D1 Chicken. Tropical Animal Science Journal 43: 197-204.
doi:10.5398/tasj.2020.43.3.197.
Allonby JIH, Philippe BW. 2018 British Poultry Standards. Wiley-Black
well.
Associaton AP. 2015 The American Standards of Perfection. Publisher:
Andesite Press.
Babar ME, Nadeem A, Hussain T, Wajid A, Shah SA, Iqbal A, Sarfraz Z,
Akram M. 2012 Microsatellite marker based genetic diversity among
four varieties of Pakistani Aseel chicken. Pak Vet J 32: 237-241.
Bashir A, Usman M, Akram M, Zahoor I, Mahmud A. 2014 Effect of age
on production performance, egg geometry and quality traits of Lakha
variety of Aseel chicken in Pakistan. J Basic & Appl Sci 10: 384-386.
doi:10.6000/1927-5129.2014.10.50.
Cheng HH, Kaiser P, Lamont SJ. 2013 Integrated Genomic approaches to
enhance genetic resistance in chickens. Annual Review of Animal Bio
sciences 1: 239-260. doi:10.1146/annurev-animal-031412-103701.
Dodgson JB, Cheng HH, Burnside JOAN. 2000 Integrating quantitative
and molecular techniques in selection for disease resistance: World’s
Poultry Congress (ed., Montreal, Canada.
Dohner JV. 2001 The encyclopedia of historic and endangered livestock
and poultry breeds. 1st edn. Yale University Press.
Drobik-Czwarno W, Wolc A, Fulton JE, Arango J, Jankowski T, O’Sulli
van NP, Dekkers JCM. 2018a Identifying the genetic basis for resis
tance to avian influenza in commercial egg layer chickens. animal 12:
Drobik-Czwarno W, Wolc A, Fulton JE, Jankowski T, Arango J, O’Sul
livan NP, Dekkers JCM. 2018b Genetic basis of resistance to avian
influenza in different commercial varieties of layer chickens. Poultry
Science 97: 3421-3428. doi:https://doi.org/10.3382/ps/pey233.
Ewald SJ, Kapczynski DR, Livant EJ, Suarez DL, Ralph J, McLeod S,
Miller C. 2011 Association of Mx1 Asn631 variant alleles with reduc
tions in morbidity, early mortality, viral shedding, and cytokine re
sponses in chickens infected with a highly pathogenic avian influenza
virus. Immunogenet 63: 363-375. doi:10.1007/s00251-010-0509-1.
Fulton JE. 2004 Selection for avian immune response: a commercial
breeding company challenge. Poult Sci 83: 658-661. doi:10.1093/
ps/83.4.658.
Goddard ME, Hayes BJ. 2009 Mapping genes for complex traits in do
mestic animals and their use in breeding programmes. Nature Re
views Genetics 10: 381-391. doi:10.1038/nrg2575.
Grimm D, Staeheli P, Hufbauer M, Koerner I, Martinez-Sobrido L, Sol
orzano A, Garcia-Sastre A, Haller O, Kochs G. 2007 Replication fit
ness determines high virulence of influenza A virus in mice carrying
functional Mx1 resistance gene. Proc Natl Acad Sci 104: 6806-6811.
doi:10.1073/pnas.0701849104.
Guo F, Zhang S, Tripathi P, Mattner J, Phelan J, Sproles A, Mo J, Wills
Karp M, Grimes HL, Hildeman D, Zheng Y. 2011 Distinct roles of
Cdc42 in thymopoiesis and effector and memory T Cell differentia
tion. PLoS ONE 6: e18002. doi:10.1371/journal.pone.0018002.
Helal M, Hany N, Maged M, Abdelaziz M, Osama N, Younan YW, Ismail
Y, Abdelrahman R, Ragab M. 2021 Candidate genes for marker-as
sisted selection for growth, carcass and meat quality traits in rabbits.
Animal Biotechnology: 1-20. doi:10.1080/10495398.2021.1908315.
Huai Y. 2008 Incubation Period for human cases of Avian Influen
za A (H5N1) Infection, China. Emerg Infect Dis 14: 1819-1821.
doi:10.3201/eid1411.080509.
Khan MT, Mahmud A, Zahoor I, Javed K. 2016 Organic and inorganic
selenium in Aseel chicken diets: Effect on hatching traits. Poult Sci
: 1466-1472. doi:10.3382/ps/pew403.
Ko JH, Jin HK, Asano A, Takada A, Ninomiya A, Kida H, Hokiyama H,
Ohara M, Tsuzuki M, Nishibori M, Mizutani M, Watanabe T. 2002
Polymorphisms and the differential antiviral activity of the chicken
Mx gene. Genome Res 12: 595-601. doi:10.1101/gr.210702.
Ko JH, Takada A, Mitsuhashi T, Agui T, Watanabe T. 2004 Native anti
viral specificity of chicken Mx protein depends on amino acid vari
ation at position 631. Anim Genet 35: 119-122. doi:10.1111/j.1365
2004.01096.x.
Koerner I, Kochs G, Kalinke U, Weiss S, Staeheli P. 2006 Protective role
of beta interferon in host defense against Influenza A virus. J Virol 81:
-2030. doi:10.1128/jvi.01718-06.
Kumar S, Stecher G, Tamura K. 2018 MEGA7: Molecular evolutionary
genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:
-1874.
Lenschow DJ, Lai C, Frias-Staheli N, Giannakopoulos NV, Lutz A, Wolff
T, Osiak A, Levine B, Schmidt RE, Garcia-Sastre A, Leib DA, Pe
kosz A, Knobeloch KP, Horak I, Virgin HW. 2007 From the cover:
IFN-stimulated gene 15 functions as a critical antiviral molecule
against Influenza, Herpes, and Sindbis viruses. Proc Natl Acad Sci
: 1371-1376. doi:10.1073/pnas.0607038104.
Liu Q, Mena I, Ma J, Bawa B, Krammer F, Lyoo YS, Lang Y, Morozov
I, Mahardika GN, Ma W, García-Sastre A, Richt JA. 2015 Newcastle
disease virus-vectored H7 and H5 live vaccines protect chickens from
challenge with H7N9 or H5N1 Avian Influenza viruses. J Virol 89:
-7408. doi:10.1128/jvi.00031-15.
Liu T, Qu H, Luo C, Li X, Shu D, Lund MS, Su G. 2014 Genomic selection
for the improvement of antibody response to Newcastle disease and
avian influenza virus in chickens. PLoS ONE 9: e112685-e112685.
doi:10.1371/journal.pone.0112685.
Luo C, Qu H, Ma J, Wang J, Li C, Yang C, Hu X, Li N, Shu D. 2013
Genome-wide association study of antibody response to Newcastle
disease virus in chicken. BMC Genet 14: 42. doi:10.1186/1471-2156
-42.
Murree B, Nizamani Z, Leghari I, Soomro N, Tariq M, Parveen F. 2016
Pathology and transmission of experimental velogenic viscerotropic
Newcastle disease in wild pigeons, broiler and Aseel chickens. Sci
Intl 28: 3965-3971.
Psifidi A, Banos G, Matika O, Desta TT, Bettridge J, Hume DA, Dessie
T, Christley R, Wigley P, Hanotte O, Kaiser P. 2016 Genome-wide
association studies of immune, disease and production traits in in
digenous chicken ecotypes. Genet Sel Evol 48: 74-74. doi:10.1186/
s12711-016-0252-7.
Rajkumar U, Haunshi S, Paswan C, Raju MVLN, Rama Rao SV, Chatter
jee RN. 2017 Characterization of indigenous Aseel chicken breed for
morphological, growth, production, and meat composition traits from
India. Poult Sci 96: 2120-2126. doi:10.3382/ps/pew492.
Rajkumar U, Muthukumar M, Haunshi S, Niranjan M, Raju MVLN,
Rama Rao SV, Chatterjee RN. 2016 Comparative evaluation of car
cass traits and meat quality in native Aseel chickens and commercial
broilers. Br Poult Sci 57: 339-347. doi:10.1080/00071668.2016.116
Rowland K, Wolc A, Gallardo RA, Kelly T, Zhou H, Dekkers JCM, Lam
ont SJ. 2018a Genetic analysis of a commercial egg laying line chal
lenged With Newcastle Disease virus. Front. Genet. 9. doi:10.3389/
fgene.2018.00326.
Sambrook J, Fritsch E, Maniatis T. 1989 Molecular cloning: a laboratory
manual Harbor Cold Spring Harbor Laboratory Press, New York.
Sartika T, Sulandari S, Zein MS. 2011 Selection of Mx gene genotype as
genetic marker for Avian Influenza resistance in Indonesian native
chicken. BMC Proc 5: S37. doi:10.1186/1753-6561-5-s4-s37.
Seyama T, Ko JH, Ohe M, Sasaoka N, Okada A, Gomi H, Yoneda A, Ueda
J, Nishibori M, Okamoto S, Maeda Y, Watanabe T. 2006 Population
research of genetic polymorphism at amino acid position 631 in
chicken Mx protein with differential antiviral activity. Biochem Gen
et 44: 432-443. doi:10.1007/s10528-006-9040-3.
Sironi L, Williams JL, Moreno-Martin AM, Ramelli P, Stella A, Jianlin H,
Weigend S, Lombardi G, Cordioli P, Mariani P. 2008 Susceptibility
of different chicken lines to H7N1 highly pathogenic avian influenza
virus and the role of Mx gene polymorphism coding amino acid po
sition 631. Virology 380: 152-156. doi:10.1016/j.virol.2008.07.022.
Sivaraman G, Kumar S. 2013 Study of disease resistance patterns of broil
er chicken challenge with avian pathogenic E. coli strain. Indian J Poult Sci 48: 68-72.
Smith J, Smith N, Yu L, Paton IR, Gutowska MW, Forrest HL, Danner
AF, Seiler JP, Digard P, Webster RG, Burt DW. 2015 A comparative
analysis of host responses to avian influenza infection in ducks and
chickens highlights a role for the interferon-induced transmembrane
proteins in viral resistance. BMC Genom 16: 574. doi:10.1186/
s12864-015-1778-8.
Smits K, Iannucci V, Stove V, Van Hauwe P, Naessens E, Meuwissen
PJ, Arien KK, Bentahir M, Plum J, Verhasselt B. 2010 Rho GTPase
CDC42 is essential for human T-cell development. Haematologica
: 367-375. doi:10.3324/haematol.2009.006890.
Suba S, Nagarajan S, Saxena VK, Kumar M, Vanamayya PR, Rajukumar
K, Gowthaman V, Jain V, Singh DP, Dubey SC. 2015 Pathology of a
H5N1, highly pathogenic avian influenza virus, in two Indian native
chicken breeds and a synthetic broiler line. Indian J Exp Bio 53: 202
Usman M, Zahoor I, Basheer A, Akram M, Mahmud A. 2014 Aseel chick
en - A preferable choice for cost-effective and sustainable production
of meat-type poultry in the tropics. Sci Intl 26. doi:20153110296.
Uyeki TM, Katz JM, Jernigan DB. 2017 Novel influenza A viruses and
pandemic threats. The Lancet 389: 2172-2174. doi:10.1016/s0140
(17)31274-6.
Wang Y, Wang J, Li BH, Qu H, Luo CL, Shu DM. 2013 An association
between genetic variation in the roundabout, axon guidance receptor,
homolog 2 gene and immunity traits in chickens. Poult Sci 93: 31-38.
doi:10.3382/ps.2013-03512.
Zhang L, Li P, Liu R, Zheng M, Sun Y, Wu D, Hu Y, Wen J, Zhao G.
The identification of loci for immune traits in chickens using a
genome-wide association study. PLoS ONE 10: e0117269.
Zhao C, Pu J. 2022 Influence of Host Sialic Acid Receptors Structure
on the Host Specificity of Influenza Viruses. Viruses 14: 2141.
doi:10.3390/v14102141
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