Effect of PITX2 gene polymorphism on growth traits in Awassi ewes

PDF (English)
Опубликован: июл. 11, 2024
DOI: https://doi.org/10.12681/jhvms.35024
M Bayraktar
Department of Animal Science, Cukurova University, Adana, Turkey
A Alkhammas
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq.
T Mohammed
a:1:{s:5:"en_US";s:101:"Department of Animal Production, College of Agriculture, Al-Qasim Green University, Babil 51001, Iraq";}
Аннотация

Genetics and environment influence quantitative traits. Several genes contribute to sheep's productive traits, including the paired-like homeodomain transcription factor 2 (PITX2). Therefore, the research investigated whether productive traits of Awassi ewes are associated with birth type and PITX2 gene variability. Two hundred and thirty-two ewes aged three to four years were studied (123 singleton ewes and 109 twin ewes). Phenotypic analysis was conducted by measuring body weight and body dimensions. Sheep genomic DNA was extracted and subjected to genotyping and sequencing to confirm the presence of variants derived from amplification of exon 5 of PITX2. A 382-bp amplicon displayed three genotypes, CC, CT, and TT. A comparison of PITX2 genotypes and birth types with productive traits showed that CC-type ewes had significantly heavier live body weights, birth weight, and higher body dimensions compared to CT-type and TT-type ewes. The CC genotypes were recorded strongly positive correlation with birth type, live body weight, height at back, width at shoulder, width at pelvic, chest width, and abdomen width ((r=0.81, P=0.02), (r=0.77, P=0.04), (r=0.97, P=0.02), (r=0.98, P=0.02), (r=0.96, P=0.03), (r=0.92, P=0.03), and (r=0.98, P=0.01) respectively. Sheep of the CC genotype had higher live body weights and body measurements, making them more productive. These findings provide valuable insight into the characteristics and functions of the PITX2 gene for the future of sheep breeding.

Article Details
  • Раздел
  • Research Articles
Лицензия Creative Commons

Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial» («Атрибуция — Некоммерческое использование») 4.0 Всемирная.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Abbasi MA, Ghafouri-Kesbi F (2011) Genetic (co) variance components for body weight and body measurements in Makooei sheep.
Asian-Australas J Anim Sci 24(6): 739-743.‏
Ajafar MH, Al-Thuwaini TM, Dakhel HH (2022a) Association of OLR1
gene polymorphism with live body weight and body morphometric
traits in Awassi ewes. Mol Biol Rep 1-5.‏
Ajafar MH, Kadhim AH, AL-Thuwaini TM (2022b) The Reproductive
Traits of Sheep and Their Influencing Factors. Rev Agric Sci 10: 82-
‏89.
Al-Shuhaib MBSA (2017) A universal, rapid, and inexpensive method for
genomic DNA isolation from the whole blood of mammals and birds.
J Genet 96(1): 171-176.‏
AL-Thuwaini TM (2022) Adiponectin and Its Physiological Function in
Ruminant Livestock. Rev Agric Sci 10: 115-122.‏
Al-Thuwaini TM, Al-Hadi ABA (2022) Association of lamb sex with
body measurements in single and twin on the Awassi ewes. Adv Anim
Vet Sci 10(8): 1849-1853.‏
Al-Thuwaini TM, Kareem ZA (2022) Novel missense variant L46Q of
fatty acid synthase gene and fatty acids content in Awassi sheep. Acta
Sci - Anim Sci 44.‏
Asaduzzaman M, Shahi PKJMG, Bari AFY (2020) Multi-farm evaluation
of morphometric, reproductive and productive traits of Jamuna basin
indigenous and Muzaffarnagari cross breed sheep of Bangladesh. J
Appl Anim Res 13(1): 31-50.‏
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a
genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3): 314.‏
Boujenane I, Diallo IT (2017) Estimates of genetic parameters and genetic
trends for pre-weaning growth traits in Sardi sheep. Small Rumin Res
‏61-68. 146:
Briata P, Ilengo C, Corte G, Moroni C, Rosenfeld MG, Chen CY, Gherzi
R (2003) The Wnt/β-catenin→ Pitx2 pathway controls the turnover of
Pitx2 and other unstable mRNAs. Mol Cell 12(5): 1201-1211.‏
Byun SO, Fang Q, Zhou H, Hickford JGH (2009) An effective method for
silver-staining DNA in large numbers of polyacrylamide gels. Anal
‏174-175. :(1)385 Biochem
Cao H, Dong X, Mao H, Xu N, Yin Z (2019) Expression analysis of the
PITX2 gene and associations between its polymorphisms and body
size and carcass traits in chickens. Animals 9(12): 1001.‏
Ghanem N, Zayed M, Mohamed I, Mohammady M, Shehata MF (2022)
Co-expression of candidate genes regulating growth performance and
carcass traits of Barki lambs in Egypt. Trop Anim Health Prod 54(5):
‏1-13.
Haldar A, Pal P, Rajesh MD, Pal SK, Majumdar D, Biswas CK, Pan S
(2014) Prolificacy and its relationship with age, body weight, parity, previous litter size and body linear type traits in meat-type goats.
Asian-Australas J Anim Sci 27(5): 628-634.‏
Kumar S, Dahiya SP, Malik ZS, Patil CS (2018) Prediction of body weight
from linear body measurements in sheep. Indian J Anim Res 52(9).‏
Mohammed MH, Al-Thuwaini TM, Al-Shuhaib MBS (2022) High association of a novel variant in the adiponectin gene with the litter size in
Awassi ewes. J Saudi Soc Agric Sci 21(5): 296-301.
Putra WPB, Agung PP, Anwar S, Said S (2019) Polymorphism of bovine
growth hormone receptor gene (g. 3338A> G) and its association with
body measurements and body weight in Pasundan cows. Trop Anim
Sci J 42(2): 90-96.‏
Wang F, Chu M, Pan L, Wang X, He X, Zhang R, Tao L, La Y, Ma L, Di
R (2021) Polymorphism detection of GDF9 gene and its association
with litter size in Luzhong mutton sheep (Ovis aries). Animals 11(2):
‏571.
Yan H, Jiang E, Zhu H, Hu L, Liu J, Qu L (2018) The novel 22 bp insertion mutation in a promoter region of the PITX2 gene is associated
with litter size and growth traits in goats. Arch Anim Breed 61(3):
‏329-336.
Zhang S, Xu H, Kang Z, Cai H, Dang R, Lei C, Chen H, Guo X, Lan
X (2018) Bovine pituitary homeobox 2 (PITX2): mRNA expression
profiles of different alternatively spliced variants and association
analyses with growth traits. Gene 669: 1-7.‏
Zhang S, Zhao H, Lei C, Pan C, Chen H, Lin Q, Lan X (2020) Effects
of genetic variations within goat PITX2 gene on growth traits and
mRNA expression. Anim Biotechnol 31(2): 107-114.‏
Zhao H, Wu X, Cai H, Pan C, Lei C, Chen H, Lan X (2013) Genetic variants and effects on milk traits of the caprine paired-like homeodomain
transcription factor 2 (PITX2) gene in dairy goats. Gene 532(2): 203-
Zhao H, He S, Wang S, Zhu Y, Xu H, Luo R, Lan X, Cai Y, Sun X (2018)
Two new insertion/deletion variants of the PITX2 gene and their effects on growth traits in sheep. Anim Biotechnol 29(4): 27
Наиболее читаемые статьи этого автора (авторов)