| More

Determination of breakthrough maternal antibodies titres by two intermediate and two intermediate plus vaccine strains against Gumboro disease, in commercial broilers

Views: 68 Downloads: 43
P. BOUGIOUKLIS (Π. ΜΠΟΥΠΟΥΚΛΗΣ), I. GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ), P. IORDANIDIS (Π. ΙΟΡΔΑΝΙΔΗΣ)
P. BOUGIOUKLIS (Π. ΜΠΟΥΠΟΥΚΛΗΣ), I. GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ), P. IORDANIDIS (Π. ΙΟΡΔΑΝΙΔΗΣ)

Abstract


Two field trials (termed A and B) were carried out on 4 farms (groups) with 20,000 broilers each. The groups in A and Β were named Al, A2, A3, A4 and Bl, B2, B3, B4, respectively. The chicks in A were hatched from breeders of 39, 43, 46, and 56 weeks-old and in Β from 27, 31, 52, and 54 weeks-old. One vaccination to each group was administered against Gumboro in drinking water, in each trial. Intermediate plus vaccines I and II were used in groups 1 and 2 and intermediate I and II in groups 3 and 4. In A the vaccinations were conducted when the chicks were 15-days-old and in Β at 19-days-old. In both trials blood samples were collected at 8, 15, 19, 23, 30 and 37 days of age. Serum examinations by ELISA established that in groups Al, A2, A3 and A4 vaccinations were carried out in the presence of mean maternal antibody titres 7.1 log2, 7.2 log2, 6.3 log2 and 6.4 log2, respectively. A regression of antibodies up to 37 days were observed in these 4 groups. In groups Bl,  B2, B3 and B4 the vaccinations were carrie out in the presence of mean maternal antibody titres 7.3 log2, 7.4 log2,3.2 log2 and 3.3 log2, respectively. A strong serological response from 30 days was observed in these 4 groups. It is remarkable that intermediate plus vaccines to 7.3log2 and 7.41og2 mean maternal antibody titres caused active immune response in groups Bl and B2, respectively, contrary to what happened to similar titres in groups Al and A2. This important observation showed that age may be one of the main factors that influences the effectiveness of vaccinations, as well as the interference of maternal antibodies and the virulence of vaccines strains. The results of both field trials indicate that both intermediate vaccines at 15-days-old did not breakthrough mean maternal antibody titres higher than 6.3 log2, whereas intermediate plus vaccines succeded to breakthrough mean maternal antibody titres lower than 7.4 log2 at 19-days-old.


Keywords


Gumboro; vaccines; interference; maternal antibodies; broilers

Full Text:

PDF

References


Bougiouklis AP (2000). A contribution to the study of immunoprotection against IBDV in chickens. Doctoral thesis.

Bougiouklis AP, Georgopoulou I, Iordanidis Ρ (2000). Epizootic study of IBD in chickens durig the years 1990-1998 in North Greece. Bulletin of the Hellenic Veterinary Medical Society, 51, (3):221-224.

Conte S and Borne Ρ (2001). Monitoring vaccination with ELISA analysis. World Poultry, Elsevier International, The Netherlands, special issue (October):20-23

Gardin Y (1991). Monitoring infectious bursal disease vaccination using ELISA serology. Zootecnica International, (April):68-70

Giamborne J J, Yu M & Eckman M (1982). Field trials with an oil emulsion infectious bursal disease vaccine in broiler breeder pullets. Poultry Science, 61:1823-1827

Giamborne JJ, Clay R (1986). Vaccination of Day-old broiler chicks against Newcastle disease and infectious bursal disease using commercial live and/or inactivated vaccines. Avian Diseases, 30:557-561

Fadly AM, Winterfield RW, Olander HJ (1976). Role of the bursa of Fabricius in the pathogenicity of inclusion body hepatitis and infectious bursal disease virus. Avian Diseases, 20:467-477

Haddad EE, Whitfill CE, Avakian AP, Ricks CA, Andrews PD, Thoma JA, Wakenell PS (1997). Efficacy of a novel infectious bursal disease virus immune complex vaccine in broiler chickens. Avian Diseases, 41:882-889

Kouwenhoven B, Van den Bos J (1994). Control of very virulent infectious bursal disease virus (Gumboro disease) in the Netherlands with more virulent vaccines. In E.F. Kaletta, U, Heffels-Redmann & H. Lange-Herbst (Eds). Proceedings of the FirstInternational Symposium on infectious bursal disease and infectious anemia, Rauischholzhauzen, Germany (June):29-32

Luttticken D, Van Loon AAW, De Vries JHR (1994). Determination of the breakthrough titre of IBD vaccines or IBD challenge strains in MDA+ chickens. In E.F. Kaletta, U, Heffels-Redmann & H. Lange-Herbst (Eds). Proceedings of the First International Symposium on infectious bursal disease and infectious anemia, Rauischholzhauzen, Germany (June): 272-279

Nakamura T, Otaki Y, Nuova Τ (1992). Immunosuppressive effectof a highly virulent infectious bursal disease virus isolated in Japan. Avian Diseases, 36: 891-896

Maas RA, Venema S, Oei HL, Pol JMA, Classen IJTM, Huurne AAHM (2001). Efficacy of inactivated infectious bursal disease (IBD) vaccines: comparison of serology with protection of progeny chickens against IBD virus strains of varying virulence.Avian Pathology, 30:345-354

Solano W, Giamborne JJ, Williams JC, Lauerman LH, Pangala VS,Garces C (1986). Effect of maternal antibody on timing of initial vaccination of young white leghorn chickens against infectious bursal disease virus. Avian Diseases, 30:648-652

Toivanen P, Naukkarinen A, Vainio Ο (1987). What is the function of bursa Fabricius? In: Avian Immunology, 1st ed, CRC Press, Boca Raton, Florida, USA, 79

Wit JJ (2001). Counting the cost of IBDV. World Poultry, Elsevier International, The Netherlands, special issue (October):6-7

Wit JJ, Heijmans JF, Mekkes DR, Van Loon AAWM (2001). Validation of five commercial available ELISAs for the detection of antibodies against infectious bursal disease virus (serotype 1). Avian Pathology, 30:543-549

Xylouri-Frankiadaki E, M. Samouhos, D. Tambouratzis and E. Stoforos. Antibody level of Infectious Bursal Disease (IBD) in layers. Bulletin of the Hellenic Veterinary Medical Society, 49(3):182-188.

Van den Berg TP (2000). Acute infectious bursal disease in poultry: a review. Avian Pathology, 29:175-194

Van Loon AAWM, Derkx A, Steentjes AHA (2001). Field experience with Gumboro vaccination in different layer breeds. World Poultry, Elsevier International, The Netherlands, special issue (October):28-30

Van der Sluis W (2001). Determaning the optimal age for vaccination against IBDV. World Poultry, Elsevier International, The Netherlands, special issue (October):25-26


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 P. BOUGIOUKLIS, I. GEORGOPOULOU, P. IORDANIDIS