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Antibiotic resistance of Staphylococcus intermedius strains isolated from 53 natural cases of canine pyoderma

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M. N. SARIDOMICHELAKIS (Μ. Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ), A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ), N. HELIADIS (Ν. ΗΛΙΑΔΗΣ), I. GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ), L. LEONTIDES (Λ. ΛΕΟΝΤΙΔΗΣ)
M. N. SARIDOMICHELAKIS (Μ. Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ), A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ), N. HELIADIS (Ν. ΗΛΙΑΔΗΣ), I. GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ), L. LEONTIDES (Λ. ΛΕΟΝΤΙΔΗΣ)

Abstract


Samples for bacterial cultures on blood agar were obtained from the skin lesions of 53 dogs demonstrating the typical clinical and cytological features of superficial (n=17) or deep (n=36) pyoderma. None of them had received any kind of antimicrobial therapy within the previous month. The Staphylococcus looking colonies were recultured on blood and Chapman agar for staphylococcal species identification and biotyping. All 53 coagulasepositive staphylococcal isolates were eventually identified as S. intermedius. The susceptibility for each of these isolates was tested against 12 different antibiotics with the disc diffusion method. Resistance rates were found to be 0% for amoxicillin/clavulanate, 11.3% for oxacillin, 7.5% for cefalexin, 5.7% for enrofloxacin, 32.1% for erythromycin, 11.3% for tylosin, 20.8% for lincomycin, 18.9% for clindamycin, 32.1% for doxycycline, 5.7% for amicacin, 7.5% for chloramphenicol and 92.5% for trimethoprim/sulfamethoxazole. No correlation between the resistance pattern and either the age, breed, sex, and the animals' lifestyle, or the depth of skin infection, the presence or not of underlying diseases and any past treatment with antibiotics was identified.


Keywords


Staphylococcus intermedius; dog; pyoderma; antibiotic resistance

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Allaker RP, Jensen L, Lloyd DH, Lamport AI (1992). Colonization of neonatal puppies by staphylococci. Br Vet J, 148: 523-528.

Barry AL, Thornsberry C (1980). Susceptibility testing: diffusion test procedures. In: Manual of Clinical Microbiology. 3rd ed, American Society for Microbiology, Washington DC: 463-474.

Bauer AW, Kirby WM, Sherris KC, Turk M (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol, 45: 493-496.

Bettenay S, Mueller R, Dell'Osa D (1994). Efficacy of doxycycline in canine and feline pyoderma. Proc 12th Ann Cong ESVD-ECVD: 64-65.

Bourdeau Ρ (1998). Treatment of canine pyodermas with an oral long acting association: baquiloprim + sufladimethoxine (Zanquilan®). In: Advances in Veterinary Dermatology. Vol 3, Butterworth - Heinemann, Oxford: 541-542.

Bourdeau P, Blumstein Ρ (1998). Comparison of two methods in the evaluation of antibiotics in canine pyodermas. Example with the associations amoxicillin + clavulanic acid (Synulox®) and

baquiloprim + sulphadimethoxin (Zanquilan®). Proc 15th Ann Cong ESVD-ECVD: 169-170.

Cerundolo R, de Caprariis D, Manna L, Gravino AE (1998). Recurrent deep pyoderma in German shepherd dogs with underlying ehrlichioses and hypergammaglobulinaemia. Vet Dermatol, 9:135-142.

Conceicao LG, Figueiredo G, Fabris VE, Lopes CAM, Franco SRVS (1998). Identification of bacterial flora and antimicrobial staphylococcal sensitivity from canine pyoderma in Brazil. In:

Advances in Veterinary Dermatology. Vol 3, Butterworth - Heinemann, Oxford: 546-547.

Cox HU, Hoskins JD, Riy AF, Newman SS, Luther DG (1984). Antimicrobial susceptibility of coagulase-positive staphylococci isolated from Louisiana dogs. Amer J Vet Res, 45: 2039-2042.

DeBoer DJ (1997). Recurrent canine pyoderma: predisposing factors and diagnostic approach. Proc 14th Ann Cong ESVD - ECVD: 9-12.

DeManuelle (2000) Deep pyoderma: getting the right drug to the right place. Compend Cont Educ Pract Vet, 22 (Suppl): 69-72.

DeSaxe M, Lloyd DH (1982). Antibiotic resistance and other characteristics of Staphylococcus aureus isolates from dogs. Assoc Vet Clin Pharmacol Therap Proc, 6: 50-54.

Frank LA, Kunkle GA (1993). Comparison of the efficacy of Cefadroxil and generic and proprietary cephalexin in the treatment of pyoderma in dogs. J Am Vet Med Assoc, 203:530-533.

Harvey RG, Noble WC (1994). A temporal study comparing the carriage of Staphylococcus intermedius on normal dogs with atopic dogs in clinical remission. Vet Dermatol, 5: 21-25.

Harvey RG, Marples RR, Noble WC (1994). Nasal carriage of Staphylococcus intermedius in humas in contact with dogs. Microb EcolHlth Dis, 7: 225-227.

Harvey RG, Hunter PA (1999). The properties and use of penicillins in the veterinary field, with special reference to skin infections in dogs and cats. Vet Dermatol 10:177-186.

Hinton M, Marston M, Hedges R (1978). The antibiotic resistance of pathogenic staphylococci and streptococci isolated from dogs. J Small Anim Pract, 19: 229-235.

Hirsh DC, Ruehl WW (1984). A rational approach to the selection of an antimicrobial agent. J Amer Vet Med Assoc, 185: 1058- 1061.

Hoie S, Fossum Κ (1989). Antibodies to staphylococcal DNAses from different animal species, including humans. J Clin Microbiol, 27: 2444-2447.

Holm B, Raue H, Bergstrom Κ, Peterson U, Morner A (1997). Antibiotic susceptibility of staphylococci isolated in Sweden from primary and recurring canine pyoderma. Proc 14th Ann Cong ESVD-ECVD: 192.

Hosmer BW, Lemeshow S (1989). Applied Logistic Regression. John Willey and Sons, New York: 106-118.

Ihrke Ρ J (1984). Therapeutic strategies involving antimicrobial treatment of the skin in small animals. J Amer Vet Med Assoc, 185:1165-1168.

Ihrke PJ (1987). An overview of bacterial skin disease in the dog. Brit Vet J, 143:112-118.

Ihrke PJ (1996). Bacterial Skin Disease in the Dog. A Guide to Canine Pyoderma. Bayer Inc.

Kloos WE (1980). Natural populations of the genus Staphylococcus. Ann Rev Microbiol, 34: 559-592.

Kloos WE, Wolfshohl JF (1982). Identification of the Staphylococcus species with the API STAPH - IDENT system. J Clin Microbiol, 16:509-516.

Kruse H, Hofshagen M, Thoresen SI, Bredal WP, Vollset I, Soli NE (1996). The antimicrobial susceptibility of Staphylococcus species isolated from canine dermatitis. Vet Res Com, 20: 205-214

Kunkle GA (1987). New considerations for rational antibiotic therapy of cutaneous staphylococcal infection in the dog. Semin Vet Med Surg, 2: 212-220.

Lloyd DH (1995). Update on pyoderma and yeast dermatitis. In: 19th Annual Waltham/OSU Symposium for the Treatment of Small Animal Diseases - Dermatology. Waltham, USA Ine: pp. 7-11.

Lloyd DH, Lamport AI, Feeney C (1996). Sensitivity to antibiotics amongst cutaneous and mucosal isolates of canine pathogenic staphylococci in the UK, 1980-96. Vet Dermatol, 7:171-175.

Lloyd DH, Lamport AI, Noble WC, Howell SA (1999). Fluoroquinolone resistance in Staphylococcus intermedius. Vet Dermatol, 10: 249-251.

Mason IS (1991). Canine pyoderma. J Small Anim Pract, 32: 381-386.

Mason IS (2001). Antibiotic selection in practice. Proc 17th Ann Cong ASVD-ACVD: 57-60.

Medleau L, Long RE, Brown J, Miller WH (1986). Frequency and antimicrobial susceptibility of Staphylococcus species isolated from canine pyodermas. Am J Vet Res, 47: 229-231.

Morner AP, Bergstrom Κ, Greko C, Franklin A (1996). Antibiotic sensitivity of staphylococci isolated from cases of canine pyoderma. Comend Cont Educ Pract Vet, 18 (Suppl): 48.

Naidoo J, Lloyd DH (1984). Transmission of genes between staphylococci on skin. In: Antimicrobials and Agriculture. Butterworths, London: 284-292.

Noble WC, Kent LE (1992). Antibiotic resistance in Staphylococcus intermedius isolated from cases of pyoderma in the dog. Vet Dermatol, 3: 71-74.

Noli C, Houwers D, Willemse Τ (1995). Study of the resistance patterns of Staphylococcus spp. isolated from dogs with pyoderma. Proc 12th Ann Cong ESVD-ECVD: 216.

Noli C (1998). Systemic antibacterial therapy. Proc 15th Ann Cong ESVD-ECVD: pp. 43-47.

Noli C, Boothe D (1999). Macrolides and lincosamides. Vet Dermatol, 10: 217-223.

Pedersen K, Wegener HC (1995). Antimicrobial susceptibility and rRNA gene restriction patterns among Staphylococcus intermedius from healthy dogs and from dogs suffering from pyoderma or otitis externa. Acta Vet Scand, 36: 335-342.

Pedersen Morner A, Bergstrom Κ, Greko C, Franklin A (1996). Antibiotic sensitivity of Staphylococci isolated from cases of canine pyoderma. Compend Cont Educ Pract Vet, 18 (Suppl): 48.

Pellerin JL, Bourdeau P, Navarro A (1997). Epidemiosurveillance of antimicrobial resistance of Staphylococcus intermedius isolates from dogs. Proc 14th Ann Cong ESVD - ECVD: 198.

Phillips WE, Williams Β J (1984). Antimicrobial susceptibility patterns of cacine Staphylococcus intermedius isolated from veterinary clinical specimens. Amer J Vet Res, 45: 2376-2379.

Piriz S, De La Fuente R, Valle J, Mateos E, Hurtado MA, Cid D, Ruiz-Santaquitteria JA, Vadillo S (1995). Comparative in vitro activity of 11 ß-lactam antibiotics against Staphylococcus intermedius strains isolated from staphylococcal dermatitis in dogs. J Vet Med B, 42: 293-300.

Quinn PJ, Carter ME, Markey BK, Carter GR (1994). Staphylococcus species. In: Clinical Veterinary Microbiology. Wolfe Publishing, Spain: 118-126.

Reedy LM (1995). Comparative results of 100 antibiotic sensitivity tests on Staphylococcus intermedius (1982-1994). Proc 11th Meeting AAVD. - ACVD: 91.

Roy AF, Newman SS, Cox HU, Hoskins JD (1984). Effect of betalactamase of Staphylococcus intermedius on disk agar diffusion susceptibility tests. Cornell Vet, 74: 354-360.

Saijonmaa Koulumies LE, Lloyd DH (1996). Colonization of canine skin with bacteria. Vet Dermatol, 7:153-162.

Saijonmaa-Kouloumies LE, Lloyd DH (2002). Colonization of neonatal puppies by Staphylococcus intermedius. Vet Dermatol,13:123-130.

Scott DW, Miller WH, Cayatte SM, Bagladi MS (1994). Efficacy of tylosin tablets for the treatment of pyoderma due to Staphylococcus intermedius infection in dogs. Can Vet J, 35: 617-621.

Scott DW, Miller WHJr, Rothstein SE, Bagladi MS (1996). Further studies on the efficacy of tylosin tablets for the treatment of pyoderma due to Staphylococcus intermedius infection in dogs. Can Vet J, 37: 617-618.

Scott DW (1997). Enrofloxacin-resistant S. intermedius. Vet Dermatol, 8:144.

Scott DW, Miller WH, Griffin CE (2001). Bacterial skin diseases. In: Small Animal Dermatology. 6th ed, WB Saunders, Philadelphia: 274-335.

Statistical Analysis Systems (2000). SAS Release 8e, SAS Institute, Cary, NC.

Stegemann M, Heukamp U, Scheer M, Krebber R (1996). Kinetics of antibacterial activity after administration of enrofloxacin in dog serum and skin: in vitro susceptibility of field isolates. Compend Cont Educ Pract Vet, 18 (Suppl): 30-34.

Schwarz S, Noble WC (1999). Aspects of bacterial resistance to antimicrobials used in veterinary dermatological practice. Vet Dermatol, 10:163-176.

Wegener HC, Pedersen Κ (1992). Variations in antibiograms and plasmid profiles among multiple isolates of Staphylococcus intermedius from pyoderma in dogs. Acta Vet Scand, 33: 391-394.

Woldehiwet Z, Jones JJ (1990). Species distribution of coagulasepositive staphylococci isolated from dogs. Vet Ree, 126: 485.


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