Antibiotic resistance and distribution of SodCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry

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Published: Jan 31, 2018
DOI: https://doi.org/10.12681/jhvms.15589
Keywords:
S. enteritidis plasmid analysis sodCI sopE and sefA genes blaTEM-1 blaTEM-2 genes
L. V. EKATERINIADOU (Λ.Β. ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ)
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA
E. BOUKOUVALA (Ε. ΜΠΟΥΚΟΥΒΑΛΑ)
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA
R. M. PAPI (Ρ.Μ. ΠΑΠΗ)
Department of Chemistry, Aristotle University of Thessaloniki
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ)
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA
V. GIANTZI (Β. ΓΙΑΝΤΖΗ)
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA
D. A. KYRIAKIDIS (Δ.Α. ΚΥΡΙΑΚΙΔΗΣ)
Department of Chemistry, Aristotle University of Thessaloniki
Abstract

Present work aimed to examine the antibiotic resistance of the Salmonella enterica serotype Enteritidis (SE) isolates from poultry, to study the plasmid-mediated ampicillin resistance and to detect and determine the distribution of sodCI, sopE and sefA genes. Thirty-five SE isolates from one-day chicks, layers and broilers were studied for susceptibility/resistance to sixteen antimicrobial agents; 23 (65.7%) of them showed resistance to ampicillin, 5 (14.3%) to ampicillin and tetracycline, 4 (11.45%) to tetracycline and 1 (2.9%) isolate showed multi-drug resistance. Ampicillin (AmpR) and ampicillin/tetracycline (AmpRTeR) resistance was easily transferred by conjugation, and all isolates except two possessed a common band. The molecular mass of the plasmid carrying ampicillin resistance was approximately determined at 41kb after DNA digestion with BamHI, HindIII, EcoRI, EcoRV and PstI restriction enzymes and ligation of EcoRI fragments to pET29c. For the detection of TEM-1 or/and TEM-2 ß-lactamases, two pairs of primers were used in a polymerase chain reaction (PCR). The PCR products showed the presence of blaTEM-1 gene in all isolates. The presence of SodCI, sopE, sefA genes was also examined by PCR. Twenty-two (62,8%) isolates carried the sodCI gene, thirty-four (97,2%) isolates carried the sopE gene and all isolates carried the sefA fimbrial locus.

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