Prevalence of bovine mastitis pathogens and antibioresistance of Escherichia coli isolates

PDF
Published: Dec 6, 2017
DOI: https://doi.org/10.12681/jhvms.15162
Keywords:
Bovine Mastitis Antibacterials Antibioresistance Escherichia coli
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ)
Veterinary Research Institute of Thessaloniki, National Agricultural Research Foundation
P. TSAKOS (Π. ΤΣΑΚΟΣ)
Center of Veterinary Institutes of Thessaloniki, Institute of Infectious and Parasitic Diseases.
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)
Center of Veterinary Institutes of Thessaloniki, Institute of Infectious and Parasitic Diseases.
Abstract

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.

Article Details
  • Section
  • Research Articles

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Barrow PA, Hill AW (1989) The virulence characteristics of strains of Escherichia coli isolated from cases of bovine mastitis in England and Wales. Vet Microbiol, 20:35-48
Bishop JR, Bodine AB, Janzen JJ (1980) Sensitivities to antibiotics and seasonal occurrence of mastitis pathogens. J Dairy Sci, 63:1134-1137
Boulton MG, Ross GW (1977) Resistance of cephoxazolebenzylpenicillin combinations to destruction by beta-lactamases associated with bovine mastitis. J Comp Pathol, 87:145-149
Carter GR, Chengappa MM (1991) Antimicrobial drugs. In: Essentials of Veterinary Bacteriology and Mycology. 4th ed, Lea & Febiger, : 91-94
Chengappa MM (1990) Antimicrobial agents and susceptibility testing. In: Diagnostic Procedures in Veterinary Bacteriology and Mycology. 5th ed, Academic Press,: 479-492
Chamings RJ (1984) The effect of not treating mild cases of clinical mastitis in a dairy herd. Vet Ree, 115:499-500
Daniel RC, O'Boyle D, Marek MS, Frost AJ (1982) A survey of clinical mastitis in South-East Queensland dairy herds. Aust Vet J, 58:143-147
Eberhart RJ (1984) Coliform mastitis Vet Clin North Am Large Anim Pract, 6:287-300
Gonzalez RN, Jasper DE, Kronlund NC, Farver TB, Cullor JS, Bushneil RB, Dellinger JD (1990) Clinical mastitis in two California dairy herds participating in contagious mastitis control programs. J Dairy Sci, 73:648-660
Hartmann Η (1990) Rates of resistance of mastitis pathogens from cows in Switzerland. Schweiz arch Tierheilkd, 132:325-329
Hill AW (1994) Escherichia coli mastitis. In: CL Gyles, Escherichia coli in Domestic Animals and Humans,
Honkansen-Buzalski T, Myllys V, Pyorala S (1994) Bovine clinical mastitis due to coagulase negative Staphylococci and their susceptibility to antimicrobiols. J Vet Med B, 41:344-350
Iliadis N, Petridou Ev, Foukos A (1997) Clinical and subclinical bovine mastitis in area of Kilkis. Bui Hel Vet Med Soc, 48:32-36
Kremer WD, Lohuis JA, Schukken YH (1989) Escherichia coli mastitis in cattle.III. Antibacterial therapy. Tijdschr Diergeneeskd, 114:939-948
Linton AH, Howe K, Sojka WJ, Wray C (1979) A note on the range of Escherichia coli O-serotypes causing clinical bovine mastitis and their antibiotic resistance spectra. J Appi Bacteriol, 46:585-590
Miltenburg JD, de Lange D, Crauwels APP, Bongers JH, Tielen MJM, Schukken YH, Elbers ARW (1996) Incidence of clinical mastitis in a random sample of dairy herds in the southern Netherlands. Vet Ree, 139:204-207
Pearson JK, Mackie DP (1979) Factors associated with the occurrence, cause and outcome of clinical mastitis in dairy cattle.Vet Ree, 105:456-463
Quinn PJ, Carter ME, Markey BK, Carter GR (1994) Mastitis. In: Clinical Veterinary Microbiology. Mosby,: 327-344
Schalm OW, Carroll EJ, Jain NC (1971) Bovine Mastitis. Lea and Febiger, Philadelphia
Schukken YH, Grommers FJ, Geer D van de, Brand A (1989) Incidence of clinical mastitis on farms with low somatic cell counts in bulk milk. Vet Ree, 125:60-63
Steras O, Aursj J, Gronnings Aeter Gjul G, Jorstad A (1994) Effect of dry-cow therapy on subclinical mastitis - an evaluation of long-acting and short-acting intramammaria J Vet Med B,
:529-540
Sears PM, Smith BS, English PB, Herer PS, Gonzalez RN (1990) Shedding pattern of Staphylococcus aureus from bovine intramammary infections. J Dairy Sci, 73:2785-2789
Smith KL, Todhunter DA, Schoenberger PS (1985) Environmental pathogens and intramammary infection during the dry period. J Dairy Sci, 68:402-417
Verheijden JH, van Miert AS (1985) Therapeutic aspects of coli mastitis in ruminants. Vet Med Nauki, 22:40-47
Wang SM, Peighton MA, Capstick JA, Geiraty (1999) Epidemiological typing of bovine streptococci by pulse field gel electrophoresis. Epidemiol Infect 123: 317-324
Watts JL (1988) Etiological agents of bovine mastitis. Vet Microbiol, 16:41-66
Weigt U, Bleckmann E (1977) Sensitivity tests and therapy of mastitis. Dtsch Tierarztl Wochenschr, 84:234-235
White ME, Montgomery ME (1987) The resemblance of clinical attributes between mastitic cows with no growth on bacterial milk cultures and those with gram-positive bacteria cultured. Can J Vet Res, 51:181-184
Wilesmith JW, Frances PG, Wilson CD (1986) Incidence of clinical mastitis in a cohort of British dairy herds. Vet Ree, 118:199-204
Wilson CD, Agger N, Gilbert GA, Thomasson CA, Tolling ST (1986) Fields trials with cefoperazone in the treatment of bovine clinical mastitis. Vet Ree, 118:17-19
Wilson CD, Gilbert GA (1986) Pharmacokinetics of cefoperazone in the cow by the intrammary route and its effect on mastitis pathogens in vitro. Vet Ree, 118:607-609
Ziv G (1976) Activity of four cephalosporin antibiotics in vitro against bovine udder pathogens and pathogenic bacteria isolated from newborn calves. Antimicrob Agents Chemother, 9:418-421
Most read articles by the same author(s)