Yeasts as a causative agent of bovine mastitis in Greece


Published: Jan 25, 2018
Keywords:
Bovine mycotic mastitis yeast Candida spp.
E. BOURTZI-HATZOPOULOU (Ε. ΜΠΟΥΡΤΖΗ-ΧΑΤΖΟΠΟΥΛΟΥ)
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ)
E. PETRIDOU (Ε. ΠΕΤΡΙΔΟΥ)
G. FILIOUSIS (Γ. ΦΙΛΙΟΥΣΗΣ)
Abstract

The aim of this study was to isolate fungi from mastitic milk of dairy cows and to identify fungal microorganisms involved in bovine mastitis. A total of 608 milk samples from clinical mastitis quarters from 580 animals in Northern Greece were collected, during the years 1997-2001. Antibacterial treatment was administrated to 50% of the sampled animals. Forty two (42) fungi were isolated and identified using mycological media and methods. From the 42 fungi isolates, 38 were yeasts and 4 moulds. The yeasts isolated were classified into the genera Candida, Geotrichum, Rhodotorula. From the thirty four (34) Candida species, 14 were identified as G tropicalis and 6 as G krusei. Furthermore, 4 isolates were classified as G pseudotropicalis, 4 as G albicans, 3 as G parapsilosis and 3 as G rugosa. Geotrichum candidum and Rhodotorula spp. were represented with 2 isolates. The isolated moulds were classified into the genus Aspergillus. Fungi were isolated in pure culture from 38 milk samples and in mixed culturewith bacteria from 4. In 510 samples only bacteria were cultured, while in 56 samples no growth of microorganism was observed. The results of the present study indicate that a level of 6.9% of mycotic mastitis is significant and yeasts are apparently implicated in mammary gland pathology causing economic loss.

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References
Awad, F., Mola, Α., Fayed, A , Abd, el-Halim., Refai, M, (1980). Studies of mycotic mastitis in Egypt. J Egyptian Vet Med Ass: 40(3) : 35-41
Barnett, JA., Pague, R.W., Yarrow, D., (1990). Yeasts characteristics and identification. Cambridge University Press, Second ed Bellani, L., Nardelli, L., Boni, P., 1973. La mammite bovine en Italie du Nord. Bull Off Int Epizootes 79:1109-17
Costa, E.O., Gandra, CR., Pires, M.F., Coutinho, S.D., Castilho, W., Teixeira, CM., (1993). Survey of bovine mycotic mastitis in dairy herds in the State of Sao Paulo, Brasil Mycopathologia. 124:13-17
Chengappa, M.M., Maddux, R.L., Greer, S.C., Pincus, LH., Geist, L.L., (1984). Isolation and identification of yeasts and yeasts-like organisms from clinical veterinary sources. J Clin Microbiol 19:427-28
Chermette, R., Bussieras, J., (1993). Parasitologie Vétérinaire Fase. V: Mycologie. Ec Nat Vet d' Alfort
Elad, D., Shpigel, N.Y., Winkler, M., Klinger, L, Fuchs, V., Saran, Α., Faingold, D., (1995). Feed contamination with Candida Krusei as a probable source of mycotic mastitis in dairy cows. J Am Vet Med Assoc Sep 1 207(5):620-2
Farnsworth, R.J., Sorensen, D.K., (1972). Prevalence and species distribution of yeast in mammary glands of dairy cows in Minnesota. Can J Comp Med 36: 329-332
Fransworth, R., Sorensen, D.K., (1975). The effect of penicillin, dihydrostreptomycin and Prednisolone Treatment of Experimental Candida krusei. Can J Comp Med, 39 : 340-348
Fransworth, R.J., (1977). Significance of fungal mastitis. J Am Vet Med Assoc 170:1173-1174
Hofmann, W., Immer, J., Lanz., E., (1965). Mycotic mastitis in cattle. Wien Tierarztl Mschr: 52:385-91
Keller, B., Scheibl, P., Bleckmann, E., Hoedemaker, M., (2000). Differentiation of yeasts in mastitis milk Mycoses 43 Suppl 1:17-9
Kirk, J.H., Bartllet, P.C., (1986). Bovine mycotic mastitis. Compendium Food Animal 8:106-110
Kitamura, H., Ami, A , Fuse, K, Seo, M., Itakura, C, (1990). Chronic mastitis caused by Candida maliosa in a cow. Vet Pathol 27:465-66
Lagneau, P.E., Labtahi, K., Swinne, D., (1996). Isolation of yeasts from bovine milk in Belgium. Mycopathologia. 135 (2): 99-102
Loftsgard, G., Lindquist, K., (1960). Bovine mycotic mastitis. Acta Vet Scan 1:201-220
Menhert, B., Ernst, K., Gedek, W., (1964). Hefen als Mastitiserryer bein Rind. Zentralblatt Veterinarmed lla:97-121
Moretti, A , Pasquali, P., Mencaroni, G., Boncio, L., Piergili Fioretti, D., (1998). Relationship between cell counts in bovine milk and the presence of mastitis pathogens (yeasts and bacteria).Zentralbl Veterinarmed (B) Apr; 45(3):129-32
Prasad, L., Prasad, S., (1966). Bovine mastitis caused by a Yeast in India Vet Ree 79 (25) 809-810
Quinn, P.J., Carter, M.E., Markey, B.K., Carter, G.R., (1994). In : Clinical Veterinary Microbiology p:327
Richard, J.L., McDonald, J.S., Fichtner, R.E„ Andersen, A.J., (1980). Identification of yeast from infected bovine mammary glands and their experimental infectivity in cattle. Am J Vet Res 41:1991-94
Singh, S.D., Thakur, D.K., Sudhan, N.A, Verna, B.B., (1992). Incidence of mycotic mastitis in cows and buffaloes. Indian Vet J 69:86-87
Sipka, M., Petrovic, D., (1975). High incidence of mycotic mastitis in cattle. Zenttralbl Veterinaermed (B) 22:353-361
Spika, M., (1957). Candida tropicalis as the cause of bovine mastitis Vet.Glasn. 11:747-51
Topolka, S., (1968). Mycotic mastitis. Vet Arh 38:242-246
Weigt U., (1970). Yeast-contaminated antibiotics as a cause of yeast mastitis. DWT 77:538-541
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