New aspects upon the classification of Staphylococcus intermedins


Published: Nov 13, 2017
Keywords:
Staphylococcus intermedius Staphylococcus pseudintermedius SIG classification dogs
E. PETRIDOU (Ε. ΠΕΤΡΙΔΟΥ)
R. FARMAKI (Ρ. ΦΑΡΜΑΚΗ)
A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ)
Abstract

In 1976, Staphylococcus intermedins was first described as being a new species isolated from pigeons, dogs, mink and horses. Until recently, the majority of coagulase-positive staphylococci originating from animals such as dogs and pigeons have been classified as S. intermedins. This species has been recognized to constitute normal skin flora of various animal species and to occasionally cause a variety of infections in dogs and cats. There have been six species of coagulase-positive staphylococci other than Staphylococcus aureus, namely S.intermedius, S.schleiferì subsp. coagulans, S.hyicus, S. lutrae, S.delphini and S. pseudintermedius. Sequence similarities of 16S rRNA genes among four of these species, except S. hyicus and S. lutrae, are 99% identical. In addition, it is very difficult to differentiate among S. intermedius, S. delphini and S. pseudintermedius phenotypically, and commercial kits are not available for the differentiation between of S. pseudintermedius and S. delphini. It has been shown that isolates phenotypically identified as Staphylococcus intermedius consist of four distinct species, including S. intermedius, Staphylococcus pseudintermedius and Staphylococcus delphini group A and B, which together represent the Staphylococcus intermedius group (SIG). Moreover, S. delphini group Β might be a novel species. In 2009, a new, rapid and inexpensive PCR-RFLP was developed and is capable of discriminating the SIG strains.

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References
Bannoehr, J., Franco Α., Iurescia M, Battisti Α., Fitzgerald R. 2009 Molecular diagnostic identification of Staphylococcus pseudintermedius. J.Clin.Microbiol.
Becker, K., C. von Eiff, B.Keller, M.Bruck, J.Etienne, and G.Peters. 2005. Thermonuclease gene as a target for specific identification of Staphylococcus intermedius isolates: use of a PCR-DNA
enzyme immunoassay. Diagn. Microbiol.Infect.Dis. 51:237-244
Devriese L.A., Vancanneyt, M., Baele M., Vaneechoutte, M., De Graef, E., Snauwaert, I., Dawyndt, P., Swings, J., Decostrere, Α., Haesebrouck F. (2005) Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals. Int.J.Syst.Evol. Microbiol. 55,1569-1573
Devriese L.A., Hermans K., Baele M., Haesebrouck F. (2009) Staphylococcus pseudintermedius versus Staphylococcus intermedius. Vet.Microbiol. 133: 206-207
Fox, G.E., J.D. Wisotzkey, and P.Jurtshuk, Jr. (1992). How close is close: 16S rRNA sequence identify may not be sufficient to guarantee species identify. Int.J.Syst.Bacteriol. 42:166-170
Freney, J., W.E.Kloos, V. Hajek and J.A.Webster (1999). Recommended minimal standards for description of new staphylococcal species. Int.J.Syst.Bacteriol. 49: 489-502
Gudding, R. 1983. Differentiation of staphylococci on the basis of nuclease properties. J.Clin. Microbiol. 18:1098-1101
Hajek, V. (1976). Staphylococcus intermedius, a new species isolated from animals. Int.J.Syst.Bacteriol.26,401-408
Harvey, R.G., Lloyd D.H. (1994) The distribution of Staphylococcus intermedius and coagulase-negative staphylococci on the hair, skin surface, within the hair follicles and on the mucus membranes of dogs. Vet. Derm. 5:75-81
Hill, P.B., Monello, K.A. (1994) Canine pyoderma. Journal of American Veterinary Association. 204:334-40
Ihrke, P.J., (1996) Bacterial skin disease in the dog: A guide to canine pyoderma. Newark, NJ: Veterinary Learning Systems. 97
Morris, D.O., Rook K.A., Shofer F.S., Rankin S.C (2006). Screening of Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus schleiferi isolates obtained from small companion animals for antimicrobial resistance: a retrospective review of 749 isolates (2003-04). Vet. Derm. 17: 332-337
Pinchbeck, L.R., Cole, L.K., Hillier, Α., Kowalski, J.J., Rajala-Schultz, P.J., Bannerman, T.L., York, S., 2006 Genotypic relatedness of staphylococcal strains isolated from pustules and carriage sites in dogs with superficial bacterial folliculitis. Am J Vet Res. 8,1337-1346
Quinn, P.J., Carter, M.E., Markey, B., Carter, G.R., 1994 Staphylococcus species. Clinical Veterinary Microbiology. Wolfe Publishing, AZ,USA,pp.ll8-126
Sasaki, T.,Kikuchi, K., Tanaka Y., Takahashi, N., Katama, S., Hiramatsu, K., 2007 Reclassification of phenotypically identified Staphylococcus intermedius strains. J.Clin.Microbiol. 45,2770-2778
Scott, D.W., Miller, W.H., Griffin, CE., 2001, Muller and Kirk's Small Animal Dermatology, 6th edn. Philadelphia: W.B. Saunders. Takahashi, T., I.Satoh and Ν Nikuchi. 1999. Phylogenetic relationships of 38 taxa of the genus Staphylococcus based 16S rRNA gene sequence analysis. Int.J.Syst.Bacteriol. 49:725-728
van Hoovels,L., Vankeerberghen, Α., Boel, Α., Van Vaerenbergh, K, De Beenhouwer, H.,2006. First case of Staphylococcus pseudintermedius infection in a human. J.Clin.Microbiol. 44,4608-4612
Varado,P.E., R.Kilpper-Balz, F.Biavaasco, G.Satta, and KH.Schleifer. 1988. Staphylococcus delphini sp.nov., a coagulase-positive species isolated from dolphins. Int.J.Syst.Bacteriol. 38: 436-439
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