Quorum Sensing System in Pathogenic Aeromonas hydrophila: Discovery of LuxRI Homologs ahyI and ahyR Genes Responsible for N-Acyl Homoserine Lactone Signal Molecules

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Veröffentlicht: Oct 21, 2024
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2024-10-21 (1)
DOI: https://doi.org/10.12681/jhvms.34042
N Filik
Süleyman Demirel Üniversitesi (Süleyman Demirel University)
https://orcid.org/0000-0003-4376-7298
E Önem
Suleyman Demirel University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Isparta, Turkey
https://orcid.org/0000-0002-7770-7958
A Kubilay
Isparta University of Applied Sciences, Faculty of Egirdir Fisheries, Isparta, Turkey
Abstract

ABSTRACT


In the pathogenesis of infections; intercellular communication system called Quorum Sensing System (QS), which regulates production of many virulence factors, is held responsible. QS genes of Aeromonas hydrophila strains that cause Motile Aeromonas Septicemia in fish was investigated by PCR method in reserach. AhyI and ahyR genes responsible for virulence of 20 A. hydrophila strains and A. hydrophila ATCC 7966 reference strain isolated from disease fish were investigated by Polymerase Chain Reaction (PCR). By PCR analysis, both ahyI and ahyR genes responsible for generation of virulence in management of QS were detected in all A. hydrophila strains. In A. hydrophila strains, it initiates disease process by preventing premature stimulation of immune system by critical gene expressions such as virulence factors in management of QS. Ultimately, QS is responsible for virulence of A. hydrophila, and thus bacteria manage disease process. Keeping diseases under control by blocking communication between them by inhibiting of QS, which is critical in disease formation, is an important strategy in fight against bacteria. With identification of QS and all members of system (AHLs, AI, QSI e.g.), early diagnosis of disease is brought to agenda and it is expected to break new ground in diagnosis of fish diseases. As a result, A. hydrophila secretes AHL signal molecule with LuxI and ahyI gene, and detects AHL signal molecule with LuxR and ahyR gene. In this study, both ahyI and ahyR genes responsible for pathogenicity and virulence in management of QS were detected in all A. hydrophila strains by PCR analysis using 20 bp long primers. Thus, it has been proven that pathogen bacteria A. hydrophila possesses LuxI/LuxR-Type QS.


 


Keywords: Interbacterial communication, A. hydrophila, Quorum sensing system, ahyI and ahyR Genes, PCR

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N Filik, Süleyman Demirel Üniversitesi (Süleyman Demirel University)

Department: Department of Fish Diseases

 

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