Effect of dietary guanidinoacetic acid supplementation on antiox idant status, biochemical parameters, inflammation cytokines and growth performance in broiler chickens

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Veröffentlicht: Feb 12, 2026
DOI: https://doi.org/10.12681/jhvms.41887
M Fathi
Department of Animal Science, Payame Noor University, Tehran, Iran
Abstract

This study examined the effects of dietary guanidinoacetic acid (GAA) on biochemical parameters, antioxidant status and inflammatory responses of chicks. Three hundred 1-d-old male broiler chicks (Ross 308) were randomly assigned to fifteen pens containing 20 chicks each, which was subjected to one of three dietary treatments supplemented with different levels of guanidinoacetic acid (GAA) at levels of (control, 0, 6 and 9 g/kg). On day 42, 2 birds per cage were weighed and euthanized, and samples were collected. Dietary GAA in 6 and 9 g/kg supplementation depressed growth performance (reduced feed intake & weight gain and increased feed conversion ratio) than broilers in the control group. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), Gamma glutamyltransferase (GGT), Alkaline Phosphatase (ALP) activity, and creatinine & urea levels showed a marked significant (P<0.05) increase in 6 and 9 g/kg groups supplemented groups compared to the control group. Also, high levels of GAA significantly reduced serum Nitric oxide levels without affecting cholesterol and triglyceride. Malondialdehyde (MDA) markedly increased along with a significant decrease of glutathione peroxidase (GPx), superoxide dismutase (SOD) and in serum and liver, and catalase (CAT) in serum in both 6 and 9g/kg groups compared to control group. None of the serum and liver inflammatory parameters were affected by high levels of GAA.  In conclusion, high levels of dietary GAA supplementation can induced oxidative stress by increasing lipid peroxidation and decreasing antioxidant function in broiler chickens.

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