Μοριακή αναζήτηση της Blastocystìs sp. σε κόπρανα σκύλου στην Ελλάδα


Δημοσιευμένα: Nov 10, 2017
Λέξεις-κλειδιά:
Blastocystìs σκύλος μικροσκοπική εξέταση PCR Ελλάδα
G. SPANAKOS (Γ. ΣΠΑΝΑΚΟΣ)
E. PAPADOGIANNAKIS (Ε. ΠΑΠΑΔΟΓΙΑΝΝΑΚΗΣ)
V. KONTOS (Β. ΚΟΝΤΟΣ)
P. MENOUNOS (Π. ΜΕΝΟΥΝΟΣ)
E. VELONAKIS (Ε. ΒΕΛΟΝΑΚΗΣ)
C. KOUTIS (Χ. ΚΟΥΤΗΣ)
N. C. VAKALIS (Ν. ΒΑΚΑΛΗΣ)
Περίληψη

Η Blastocystìs είναι ένα αναερόβιο παράσιτο που έχει βρεθεί στο έντερο πολλών σπονδυλωτών, περιλαμβανομένωντου ανθρώπου και του σκύλου. Πρόσφατα, η Blastocystìs ταξινομήθηκε σε εννέα υπότυπους, βάσει των φυλογενετικών δένδρων που προέκυψαν από την ανάλυση της αλληλουχίας της μικρής υποομάδας του rRNA γονιδίου. Η παθογένεια της Blastocystìs στο σκύλο παραμένει ασαφής και η ύπαρξη παθογόνων και μη-παθογόνων υπότυπων ακόμη ερευνάται. Δείγματα κοπράνων από 30 δεσποζόμενους και 42 αδέσποτους σκύλους, προερχόμενους από την Κεντρική και Βόρεια Ελλάδα εξετάστηκαν μικροσκοπικάκαι με τη μέθοδο PCR για τυχόν ανίχνευση της Blastocystìs sp. Όλα τα δείγματα βρέθηκαν αρνητικά και με τις δύο μεθόδους. Φαίνεται ότι στην Ελλάδα ο σκύλος μάλλον δεν συνιστά «δεξαμενή» της Blastocystìs sp., αν και απαιτείταιεπιδημιολογική μελέτη μεγάλης κλίμακας για να εξαχθούν ασφαλή συμπεράσματα.

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Abe N, Nagoshi M, Takami K, Sawano Y, Yoshikawa H. (2002). A survey of Blastocystis sp. in livestock, pets and zoo animals in Japan. Veterinary Parasitology, 106: 203-212.
Albrecht H, Stellbrink HJ, Koperski K, Greten H. (1995). Blastocystis hominis in human immunodeficiency virus-related diarrhea. Scan J Gastroenterol, 30: 909-914.
Arisue N, Hashimoto T, Yoshikawa H. (2003). Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates. Parasitology, 126:1-9.
Belova LM, Kostenko LA. (1990). Blastocystis galli sp. (Protista: Phizopoda) from the intestine of domestic hens. Parasitologiia, 24:164-168.
Belova LM. (1991). Blastocystis anatis sp. nov. (Rhizopoda, Lobosea) from Anas platyrhynchos. Zool Zh, 70: 5-10.
Brumpt E. (1912). Blastocystis hominis η. sp. et formes voisines. Bull Soc Pathol Exot, 5: 725-730.
Carrascosa M, Martinez J, Perez-Castrillon JL. (1996). Hemorrhagic proctosigmoiditis and Blastocystis hominis infection. Ann Intern Med, 124: 278-279.
Chapman S, Thompson C, Wilcox A, and Russell K. (2009). What is your diagnosis? Rectal scraping from a dog with diarrhea. Vet Clin Pathol, 38(1): 59-62.
Claerebout E, Casaert S, Dalemans AC, De Wilde N, Levecke B, Vercruysse J, Geurden Τ (2009). Giardia and other intestinal parasites in different dog populations in Northern Belgium. Vet Parasitol, 161(1-2): 41-6.
Clark CG. (1997). Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol, 87: 79-83.
Daryani A, Sharif M, Amouei A, Ettehad GH, Ziaei H, Gohardehi SH, Bastani R (2008). Blastocystis spp: a Neglected Zoonotic Protozoan. Proc ASEAN Congr Trop Med Parasitol, 3: 59-62.
Duda A, Stenzel D, Boreham PF. (1998). Detection of Blastocystis sp. in domestic dogs and cats. Veterinary Parasitology, 76: 9-17.
Ho LC, Jeyaseelan K, Singh M. (2001). Use of the elongation factorla gene in a polymerase chain reaction-based restriction-fragmentlength polymorphism analysis of genetic heterogeneity among Blastocystis species. Mol Biochem Parasitol, 112: 287-291.
Inpankaew T, Traub R, Thompson R.C and Sukthana Y (2007). Canine Parasitic Zoonoses in Bangkok Temples. Southeast Asian J Trop Med Public Health, 38 (2): 247-255.
Javier Lopez D, Katia Abarca V, Patricio Paredes M, Elisa Inzunza Τ (2006). Intestinal parasites in dogs and cats with gastrointestinal symptoms in Santiago, Chile. Rev Méd Chile, 134:193-200.
Kaneda Y, Horiki N, Cheng XJ, Fujits Y, Msruyama M, Tachibana H. (2001). Ribodemes of Blastocystis hominis isolated in Japan. Am J Trop Med & Hygiene, 65: 393-396.
Levy Y, George J, Shoenfeld Y. (1996). Severe Blastocystis hominis in an elderly man. J Infect, 33: 57-59.
Menounos PG, Spanakos G, Tegos N, Vassalos CM, Papadopoulou C and Vakalis NC. (2008). Direct detection of Blastocystis sp. in human faecal samples and subtype assignment using single strand conformational polymorphism and sequencing. Mol Cell Probes, 22: 24-29.
Pakandl M. (1991). Occurence oiBlastocystis sp. in pigs. Folia Parasitol, 38: 297-301.
Parkar U, Traub RJ, Kumar S, Mungthin M, Vitali S, Leelayoova S, Morris Κ, Thompson RCA. (2007). Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology, 134(3): 359-67.
Quilez J, Sanchez-Acedo C, Clavel A, Causape AC. (1995). Occurrence of Blastocystis sp. in cattle in Aragon, northeastern Spain. Parasitol Res, 81: 703-705.
Ribes JA, Vanover-Sams CL, Baker DJ. (2000). Zygomycetes in human disease. Clin. Microbiol. Rev, 13: 236-301.
Silberman JD, Sogin ML, Leipe DD, Clark CG. (1996). Human parasite finds taxonomic home. Nature, 380: 398.
Stensvold CR, Suresh GK, Tan KSW, Thompson RCA, Traub RJ, Viscogliosi E, Yoshikawa H, Clark CG. (2007). Terminology for Blastocystis sub-types - a consensus. Trends Parasitol, 23: 93-96.
Stensvold CR, Alfellani MA, Norskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG. (2009). Sub-type distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new sub-type. International Journal of Parasitology, 39: 473-479.
Stenzel DJ, Boreham PFL. (1996). Blastocystis hominis revised. Clin Microbiol Rev, 9: 563-584
Sun T, Katz S, Tanenbaum B, Schenone C. (1989). Questionable clinical significance of Blastocystis hominis infection. Am J Gastroenterol, 84:1543-1547.
Tan KSW. (2004). Blastocystis in humans and animals: new insights using modern methodologies. Vet. Parasitol, 126:121-144.
Termmathurapoj S, Leelayoova S, Aimpun P, Thathaisong U, Nimmanon T, Taamasri P, Mungthin M. (2004). The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol Res, 93:445-447.
Windsor JJ, MacFarlane L, Hughes-Thapa G, Jones KA, Whitside TM. (2002). Incidence of Blastocystis hominis in faecal samples submitted for routine microbiological analysis. Br J Biomed Sci, 59:154-157.
Yakoob J, Jafri W, Jafri N, Khan R, Islam M, Beg MA, Zaman V. (2004). Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. Am J Trop Med Hyg, 70: 383-385.
Yoshikawa H, Nagano L, Singh M, Takahashi Y. (1996). DNA polymorphism revealed by arbitrary primers polymerase chain reaction among Blastocystis trains isolated from humans, a chicken and a reptile. J Eukaryot Microbiol, 3:127-130.
Yoshikawa H, Nagano L, Wu Z, Yap EH, Singh M, Takahashi Y. (1998). Genomic polymorphism among Blastocystis hominis strains and development of sub-type-specific diagnostic primers. Mol Cell Probes, 12:153-159.
Yoshikawa H, Abe N, Iwasaka M, Kitano S, Nagano L, Wu Z, Takahashi Y. (2000). Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. J Clin Microbiol, 38:1324-1330.
Yoshikawa H, Wu Z, Hashimoto T, Geok-Choo N, Tan KS. (2007). Ultrastructural and phylogenetic studies on Blastocystis isolates from cockroaches. J. Eukaryot. Micribiol, 54: 33-37.
Zaki M, Daud A.S, Pugh R.N.H, AI-Ali F, Al-Mutairi G, Al-Saleh Q. (1991). Clinical report of Blastocystis hominis infection in children. J Trop Med Hyg, 94:118-122.
Zheng Zhang, Scott Schwartz, Lukas Wagner and Webb Miller (2000). "A greedy algorithm for aligning DNA sequences". J Comput Biol, 7(1-2): 203-14.
Χαραλαμπίδης ΣΘ. (2003). Παρασιτικά Νοσήματα ζώων και ανθρώπου. Πρωτοζωώσεις, Ελμινθώσεις, Αρθροποδώσεις.
University Studio Press, Θεσσαλονίκη pp: 239-250.
Τα περισσότερο διαβασμένα άρθρα του ίδιου συγγραφέα(s)