Μοριακή αναζήτηση της Blastocystìs sp. σε κόπρανα σκύλου στην Ελλάδα


Δημοσιευμένα: Νοε 10, 2017
Λέξεις-κλειδιά:
Blastocystìs σκύλος μικροσκοπική εξέταση PCR Ελλάδα
G. SPANAKOS (Γ. ΣΠΑΝΑΚΟΣ)
E. PAPADOGIANNAKIS (Ε. ΠΑΠΑΔΟΓΙΑΝΝΑΚΗΣ)
V. KONTOS (Β. ΚΟΝΤΟΣ)
P. MENOUNOS (Π. ΜΕΝΟΥΝΟΣ)
E. VELONAKIS (Ε. ΒΕΛΟΝΑΚΗΣ)
C. KOUTIS (Χ. ΚΟΥΤΗΣ)
N. C. VAKALIS (Ν. ΒΑΚΑΛΗΣ)
Περίληψη

Η Blastocystìs είναι ένα αναερόβιο παράσιτο που έχει βρεθεί στο έντερο πολλών σπονδυλωτών, περιλαμβανομένωντου ανθρώπου και του σκύλου. Πρόσφατα, η Blastocystìs ταξινομήθηκε σε εννέα υπότυπους, βάσει των φυλογενετικών δένδρων που προέκυψαν από την ανάλυση της αλληλουχίας της μικρής υποομάδας του rRNA γονιδίου. Η παθογένεια της Blastocystìs στο σκύλο παραμένει ασαφής και η ύπαρξη παθογόνων και μη-παθογόνων υπότυπων ακόμη ερευνάται. Δείγματα κοπράνων από 30 δεσποζόμενους και 42 αδέσποτους σκύλους, προερχόμενους από την Κεντρική και Βόρεια Ελλάδα εξετάστηκαν μικροσκοπικάκαι με τη μέθοδο PCR για τυχόν ανίχνευση της Blastocystìs sp. Όλα τα δείγματα βρέθηκαν αρνητικά και με τις δύο μεθόδους. Φαίνεται ότι στην Ελλάδα ο σκύλος μάλλον δεν συνιστά «δεξαμενή» της Blastocystìs sp., αν και απαιτείταιεπιδημιολογική μελέτη μεγάλης κλίμακας για να εξαχθούν ασφαλή συμπεράσματα.

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Λήψεις
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Αναφορές
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