Molecular screening for Blastocystìs sp. in canine faecal samples in Greece

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Publiée : Nov 10, 2017
DOI : https://doi.org/10.12681/jhvms.14852
G. SPANAKOS (Γ. ΣΠΑΝΑΚΟΣ)
Department of Parasitology, Entomology and Tropical Diseases, National School of Public Health
E. PAPADOGIANNAKIS (Ε. ΠΑΠΑΔΟΓΙΑΝΝΑΚΗΣ)
Department of Parasitology, Entomology and Tropical Diseases, National School of Public Health
V. KONTOS (Β. ΚΟΝΤΟΣ)
Department of Veterìnary Public Health, National School of Public Health
P. MENOUNOS (Π. ΜΕΝΟΥΝΟΣ)
Department of Parasitology, Entomology and Tropical Diseases, National School of Public Health
E. VELONAKIS (Ε. ΒΕΛΟΝΑΚΗΣ)
Department of Microbiology and Applied Immunology, National School of Public Health
C. KOUTIS (Χ. ΚΟΥΤΗΣ)
Department of Public Health, Technological Education Institute of Athens
N. C. VAKALIS (Ν. ΒΑΚΑΛΗΣ)
Department of Parasitology, Entomology and Tropical Diseases, National School of Public Health
Résumé

Blastocystìs is an anaerobic parasitic microorganism which has been found in the intestinal tract of many vertebrates including humans and dogs. Recently, members of Blastocystìs sp. were classified into nine sub-types, based on phylogenetic trees derived from sequence analysis of the small sub-unit ribosomal RNA gene. The pathogenesis of Blastocystìs in the dog remains uncertain and the existence of pathogenic and non-pathogenic sub-types is under investigation. Feacal samples from 30 privately owned and 42 stray dogs, originated from central and northern Greece, were examined by both light microscopy and PCR assays for the detection of Blastocystìs sp. All samples were found to be negative by both assays. It seems that, in Greece, the dog is unlikely to be a "reservoir" of Blastocystìs sp., although a large scale epidemiologic study is needed to be carried out in order for more conclusive results to be drawn.

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