Effect of seasonal infertility period on boar sperm proteins and quality characteristics


Keywords:
boar semen semen analysis HSP90 GPX5 seasonal infertility
I.A. MICHOS
I.A. TSAKMAKIDIS
M. TSANTARLIOTOU
G. TSOUSIS
P. HADWEH
C.M. BOSCOS
Abstract
Swine seasonal infertility reduces the productivity and profitability of a pig farm. The main causes of this condition are elevated environmental temperatures and long photoperiod during the summer season. The aim of this study was to investigate which sperm proteins and parameters are affected during the period of seasonal infertility. Depending on the environmental temperatures, the period from October to June was considered as cold and the period from July to September as warm season. A total of 65 ejaculates from 18 boars were collected over a year. Each semen sample was evaluated for kinetics (Computer Assisted Semen Analyzer), morphology (Sperm Blue stain), viability (Propidium Iodide - Calcein AM stain), mitochondrial membrane potential (Rhodamine 123 – Propidium Iodide stain), membrane integrity and functionality (Hypo-osmotic swelling test) and sperm DNA integrity (Acridine Orange Test). Moreover, selected proteins (HSP90, GPX5, OPN) were detected and quantified. The kinetic parameters VSL, LIN and the midpiece abnormalities were significantly higher in the warm compared to the cold season (p<0.05), while a strong tendency towards higher values for HSP90 and GPX5 was observed in warm compared to cold season (p=0.07and p=0.06, respectively). In conclusion, among the boar sperm characteristics tested in our study, seasonal infertility period negatively affected VSL and LIN kinetics, while GPX5 seminal plasma enzyme and HSP90 sperm surface protein increased their sperm protective effects.
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References
Awda BJ, Mackenzie-Bell M, Buhr MM (2009) Reactive oxygen species and boar sperm function. Biol Reprod 81:553–561.
Barranco I, Ortega MD, Martinez-Alborcia MJ, Vazquez JM, Martinez EA, Roca J (2013) Season of ejaculate collection influences the freezability of boar spermatozoa. Cryobiology 67:299-304.
Barranco I, Tvarijonaviciute A, Perez-Patiño C, Vicente-Carrillo A, Parrilla I, et al. (2016) Glutathione Peroxidase 5 Is Expressed by the Entire Pig Male Genital Tract and Once in the Seminal Plasma Contributes to Sperm Survival and In Vivo Fertility. PLOS ONE 11:e0162958.
Basioura A, Boscos CM, Parrilla I, Tsousis G, Tsakmakidis IA (2018) Effect of astaxanthin on the quality of boar sperm stored at 17° C, incubated at 37° C or under in vitro conditions. Reprod Domest Anim 53:463-471.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM (2012) Application of computer-assisted semen analysis to explain variations in pig fertility. J Anim Sci, 90:779–789.
Cameron RD, Blackshaw AW (1980) The effect of elevated ambient temperature on spermatogenesis in the boar. J. Reprod Fertil 59:173–179.
Casas I, Sancho S, Briz M, Pinart E, Bussalleu E, Yeste M, Bonet S (2009) Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology 72:930-948.
De Rensis F, Kirkwood RN (2016) Control of oestrus and ovulation: fertility to timed insemination of gilts and sows. Theriogenology 86:1460-1466.
De Rensis F, Ziecik AJ, Kirkwood RN (2017) Seasonal infertility in gilts and sows: Aetiology, clinical implications and treatments. Theriogenology 96:111-117.
Drevet JR (2006) The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol 250:70-79.
Egbunike GN, Dede TI (1980) The Influence of Short-Term Exposure to Tropical Sunlight on Boar Seminal Characteristics. Int J Biometeor 24:129-135.
Flowers WL (1997) Management of boars for efficient semen production. J Reprod Fertil 52:67–78.
Flowers WL (2015) Factors Affecting the Efficient Production of Boar Sperm. Reprod Domest Anim 50:25-30.
González-Cadavid V, Martins JA, Moreno FB, Andrade TS, Santos AC, Monteiro-Moreira AC, Moreira RA, Moura AA (2014) Seminal plasma proteins of adult boars and correlations with sperm parameters. Theriogenology 82:697-707.
Hallak J, Sharma RK, Pasqualotto FF, Ranganathan P, Thomas AJ Jr, Agarwal A (2001). Creatine kinase as an indicator of sperm quality and maturity in men with oligospermia. Urology 58:446-451.
Hamilton TR, Mendes CM, de Castro LSD, Assis PM, Siqueira AF, Delgado JdeC, Goissis MD, Muiño-Blanco T, Cebrián-Pérez JÁ, Nichi M, Visintin JA, Assumpçao ME (2016) Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm. Oxid Med Cell Longev 2016:1687657.
Hao Y, Mathialagan N, Walters E, Mao J, Lai L, Becker D, Li W, Critser J, Prather RS (2006) Osteopontin Reduces Polyspermy During In Vitro Fertilization of Porcine Oocytes. Biol Reprod 75:726- 733.
Hao Y, Murphy CN, Spate L, Wax D, Zhong Z, Samuel M, Mathialagan N, Schatten H, Prather RS (2008) Osteopontin improves in vitro development of porcine embryos and decreases apoptosis. Mol Reprod Dev 75:291-298.
Hirai M, Boersma A, Hoeflich A, Wolf E, Foll J, Aumüller TR, Braun J (2001) Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors. J Androl 22:104-110.
Huang SY, Kuo YH, Tsou HL, Lee YP, King YT, Huang HC, Yang PC, Lee WC (2000) The decline of porcine sperm motility by geldanamycin, a specific inhibitor of heat-shock protein 90 (HSP90). Theriogenology 53:1177-1184.
Huszar G, Vigue L (1994) Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 15:71-77.
Karageorgiou MA, Tsousis G, Boscos CM, Tzika ED, Tassis PD, Tsakmakidis IA (2016) A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility. Acta Vet Brno 85:23-31.
Killian GJ, Chapman DA, Rogowski LA (1993) Fertility-associated proteins in Holstein bull seminal plasma. Biol Reprod 49:1202-1207.
Lee WY, Lee R, Kim HC, Lee KH, Cui XS, Kim NH, Lee IJ, Uhm SJ, Yoon MJ, Song H (2014) Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization. Asian-Australas J Anim Sci 27:1417-1425.
Love RJ (1978) Definition of a seasonal infertility problem in pigs. Vet Rec 103:443–446.
Najafi A, Zhandi M, Towhidi A, Sharafi M, Akbari Sharif A, Khodaei Motlagh M, Martinez-Pastor F (2013) Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology 66:275-282.
Peltoniemi OA, Love RJ, Heinonen M, Tuovinen V, Saloniemi H (1999) Seasonal and management effects on fertility of the sow: a descriptive study. Anim Reprod Sci 55:47-61.
Peña ST, Gummow B, Parker AJ, Paris DBBP (2016) Revisiting summer infertility in the pig: Could heat stress-induced sperm DNA damage negatively affect early embryo development? Anim Prod Sci 57:1975-1983.
Popwell JM, Flowers WL (2004) Variability in relationships between semen quality and estimates of in vivo and in vitro fertility in boars. Anim Reprod Sci 81:97–113.
Prunier A, Dourmad JY, Etienne M (1994) Effect of light regimen under various ambient temperatures on sow and litter performance. J Anim Sci 72:1461-1466.
Radomil L, Pettitt MJ, Merkies KM, Hickey KD, Buhr MM (2011) Stress and dietary factors modify boar sperm for processing. Reprod Dom Anim 46:39-44.
Riquet J, Gilbert H, Feve K, Labrune Y, Rose R, Billon Y, Giorgi M, Loyau T, Gourdine JL, Renaudeau D (2017) Genetic dissection of mechanisms underlying heat adaptation in pigs. In: 36th Conference of the International Society for Animal Genetics (ISAG), Illinois, U.S.A: pp.133.
Strzeżek J, Wysocki P, Kordan W, Kuklińska M, Mogielnicka M, Soliwoda D, Fraser L (2005) Proteomics of boar seminal plasma – current studies and possibility of their application in biotechnology of animal reproduction. Reprod Biol 5:279-290.
Tardif S, Laforest JP, Cormier N, Bailey JL (1999) The importance of porcine sperm parameters on fertility in vivo. Theriogenology 52:447–459.
Tejada RI, Mitchell JC, Norman A, Marik JJ, Friedman S (1984) A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril 42:87–91.
Valencia J, Gomez G, Lopez W, Mesa H, Henao FJ (2017) Relationship between HSP90a, NPC2 and L-PGDS proteins to boar semen freezability. J Anim Sci Biotechno 8:21.
Vilagran I, Castillo J, Bonet S, Sancho S, Yeste M, Estanyol JM, Oliva R (2013) Acrosin-binding protein (ACRBP) and triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity. Theriogenology 80:443-450.
Vilagran I, Castillo-Martín M, Prieto-Martínez N, Bonet S, Yeste M (2016) Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality. Anim Reprod Sci 165:22-30.
Yeste M, Bonet S, Rodríguez-Gil JE, Rivera Del Álamo MM (2018) Evaluation of sperm motility with CASA-Mot: which factors may influence our measurements? Reprod Fertil Dev 30:789-798.
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