Detection of Quorum Sensing System (Cell to Cell Communication) Using Marker Strains in Vibrio alginolyticus Strains and Determine Virulence under Master of this System
Περίληψη
ABSTRACT
There are intercellular communication and social behaviors, including Quorum Sensing System (QS) in bacteria. The present study aims to investigate the production of QS signal molecules (N-acyl homoserine lactones, AHLs) in bacterial fish pathogen Vibrio alginolyticus (3 strains) and also determine the virulence using these AHLs under the management of the QS in V. alginolyticus. QS of V. alginolyticus was proved by cross validation assay used by AHL marker bacteria strains (Agrobacterium tumefaciens NT1 and Chromobacterium violaceum CV026). V. alginolyticus was not produced N-butanoyl homoserine lactone (BHL), while N-(3-okzododekanoyl)-L-homoserine lactone (OdDHL) production was detected in V. alginolyticus. The presence of virulence factors, such as biofilm, pigment, rhamnolipid, hemolysis, elastase, protease and amylase production, was investigated as phenotypically in V. alginolyticus. V. alginolyticus was found to produce biofilm, pigment, amylase and elastase; however, it was not determined to produce rhamnolipid, hemolysis and protease virulence.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Filik, N., & Kubilay, A. (2023). Detection of Quorum Sensing System (Cell to Cell Communication) Using Marker Strains in Vibrio alginolyticus Strains and Determine Virulence under Master of this System. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 73(4), 4945–4956. https://doi.org/10.12681/jhvms.28525
- Τεύχος
- Τόμ. 73 Αρ. 4 (2022)
- Ενότητα
- Research Articles
Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.
Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.
Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.
Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc
Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
REFERENCES
Aguirre-Guzman G, Mejia Ruiz H, and Ascencio F (2004). A review of extracellular virulence product of Vibrio species important in diseases of cultivated shrimp. Aquacult Res 35:1395–1404.
Bruhn JB, Dalsgaard I. and Nielsen KF et al. (2005). Quorum sensing signal molecules (acylated homoserine lactones) in Gramnegative fish pathogenic bacteria. Diseases of Aquatic Organisms 65: 43-52.
Chang SC and Lee CY (2020). Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes in Vibrio parahaemolyticus. Frontiers in microbiology 11: 534692.
Croxatto A, Chalker VJ and Lauritz J et al. (2002). VanT, a Homologue of Vibrio harveyi LuxR, Regulates Serine, Metalloprotease, Pigment and Biofilm Production in Vibrio anguillarum. Journal of Bacteriology 184(6): 1617-1629.
Deep A, Chaudhary U and Gupta V (2011). Quorum sensing and Bacterial Pathogenicity: From Molecules to Disease. J Lab Physicians 3(1): 4–11.
Dong Y, Zhang X and Soo HL et al. (2005). The two-component response regulator PprB modulates quorum-sensing signal production and global gene expression in P. aeruginosa. Molecular Microbiology 56: 1287-1301.
Dutta T and Srivastava S (2018). Small RNA-mediated regulation in bacteria: a growing palette of diverse mechanisms. Gene 656: 60-72.
Eickhoff MJ and Bassler BL (2018). SnapShot: bacterial quorum sensing. Cell 174: 1328-1328.
Filik F (2019). Bazı bakteriyel balık patojenlerinde biyofilm oluşumuna farklı maddelerin in vitro etkisinin tespiti. T.C. Isparta Uygulamalı Bilimler Üniversitesi Lisansüstü Eğitim Enstitüsü, Yüksek Lisans Tezi, Su Ürünleri Yetiştiriciliği Anabilim Dalı.
Filik N and Kubilay A (2019). Bazı bakteriyel balık patojenlerinde biyofilm oluşumunun farklı in vitro metodlarla tespiti. Acta Aquatica Turcica 15(3): 378-390.
Filik N and Kubilay A (2020). Aeromonas hydrophila balık patojeninde biyosensör suşlar aracılığıyla çevreyi algılama sisteminin tespiti ve virülens faktörleri Determination of quorum sensing system via Monitor strains and virulence factors in fish pathogen Aeromonas hydrophila. Ege Journal of Fisheries and Aquatic Sciences 37: 29-36.
Filik N (2020). Kültür Balıklarından İzole Edilen Aeromonas hydrophila Suşlarında Fenolik Bileşenlerin Çevreyi Algılama Sistemi Üzerine İnhibisyon Etkisi ve Suşlar Arasındaki Klonal İlişkinin Pulsed Field Jel Elektroforez Yöntemiyle Belirlenmesi. TC. Isparta Uygulamalı Bilimler Üniversitesi Lisansüstü Eğitim Enstitüsü, Doktora Tezi.
Gong QY, Yang MJ, Yang LF, Chen ZG, Jiang M and Peng B (2020). Metabolic modulation of redox state confounds fish survival against Vibrio alginolyticus infection. Microbial biotechnology 13(3): 796-812.
Gu D, Liu H and Yang Z et al. (2016). Chromatin immunoprecipitation sequencing technology reveals global regulatory roles of low-cell-density quorum-sensing regulator AphA in the pathogen Vibrio alginolyticus. Journal of Bacteriology 198: 2985-2999.
Jiang Q, Chen J and Yang C et al. (2019). Quorum sensing: a prospective therapeutic target for bacterial diseases. BioMed Research International 2019: 15.
Kalia VC, Raju SC and Purohit HJ (2011). Genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and -lactonase. Open Microbiol J 5: 1–13.
Kothari V, Sharma S and Padia D (2017). Recent research advances on Chromobacterium violaceum. Asian Pacific journal of tropical medicine 10(8): 744-752.
Lafisca A, Pereira CS and Giaccone V et al. (2008). Enzymatic characterization of Vibrio alginolyticus strains isolated from bivalves harvested at Venice Lagoon (Italy) and Guanabara Bay (Brazil). Revista do Instituto de Medicina Tropical de São Paulo 50: 199-202.
Liu G, Wu S and Jin W et al. (2016). Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities. Scientific reports 6: 1-12.
Liu W, Huang L and Su Y et al. (2017). Contributions of the oligopeptide permeases in multistep of Vibrio alginolyticus pathogenesis. MicrobiologyOpen 6: e00511.
Liu H, Wang Y and Cao J et al. (2020a). Antimicrobial activity and virulence attenuation of citral against the fish pathogen Vibrio alginolyticus. Aquaculture 515: 734578.
Liu H, Liu W and He X et al. (2020b). Characterization of a cell density-dependent sRNA, Qrr, and its roles in the regulation of the quorum sensing and metabolism in Vibrio alginolyticus. Applied microbiology and biotechnology 104(4): 1707-1720.
McClean KH, Winson MK and Fish L et al. (1997). Quorum sensing and Chromobacterium violeceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology 143: 3703-3711.
Miller MB and Bassler BL (2001). Quorum sensing in bacteria. Annu Rev Microbiol 55: 165-199.
Natrah FMI, Ruwandeepika HD, Pawar S, Karunasagar I, Sorgeloos P, Bossier P and Defoirdt T (2011). Regulation of virulence factors by quorum sensing in Vibrio harveyi. Veterinary microbiology, 154(1-2): 124-129.
Ng WL and Bassler BL (2009). Bacterial quorum-sensing network architectures. Annu Rev Genet 43: 197-222.
Nickzad A, Lépine F and Déziel E (2015). Quorum sensing controls swarming motility of Burkholderia glumae through regulation of rhamnolipids. PLoS One 10: e0128509.
Nitzan M, Rehani R and Margalit H (2017). Integration of bacterial small RNAs in regulatory networks. Annu Rev Biophys 46: 131.
Ohman DE, Cryz SJ and Iglewski BH (1980). Isolation and characterization of a P. aeruginosa PAO mutant that produces altered elastase. J Bacteriol 142: 836-842.
Rashiya N, Padmini N and Ajilda AAK et al. (2021). Inhibition of biofilm formation and quorum sensing mediated virulence in Pseudomonas aeruginosa by marine sponge symbiont Brevibacterium casei strain Alu 1. Microbial Pathogenesis 150: 104693.
Ravn L, Christensen AB and Molin S et al. (2001). Methods for acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics. Journal of Microbiological Methods 44: 239-251.
Reina JC, Pérez-Victoria I and Martín J et al. (2019). A quorum-sensing inhibitor strain of Vibrio alginolyticus blocks Qs-controlled phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa. Marine drugs 17: 494.
Reilly GD, Reilly CA and Smith EG (2011) Baker-Austin C. Vibrio alginolyticus-associated wound infection acquired in British waters, Guernsey, July. Euro Surveill 16: 19994.
Rémy B, Mion S and Plener L et al. (2018). Interference in bacterial quorum sensing: a biopharmaceutical perspective. Frontiers in pharmacology 9: 203.
Siegmund I and Wagner F (1991). New methods for detecting rhamnolipids excreted by Pseudomonas species during growth in mineral agar. BioTechniques 5: 265-268.
Syed KA, Beyhan S and Correa N et al. (2009). The Vibrio cholerae flagellar regulatory hierarchy control expression of virulence factors. Journal of Bacteriology 191: 6555–6570.
Swift S, Lynch MJ and Fish L et al. (1999). Quorum Sensing-Dependent Regulation and Blockade of Exoprotease Production in Aeromonas hydrophila. Infection and Immunity 67(10): 5192-5199.
Wu TK, Wang YK and Chen YC et al (2007). Identification of a Vibrio furnissii oligopeptide permease and characterization of its in vitro hemolytic activity. Journal of Bacteriology 189: 8215-8223.
Ye J, Ma Y and Liu Q et al (2008). Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases 31: 161-169.
