Διέγερση της φλεγμονώδους απόκρισης στα κύτταρα Sertoli ορνιθίων μετά από έκθεση σε μετφορμίνη

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Δημοσιευμένα: Apr 20, 2024
DOI: https://doi.org/10.12681/jhvms.33979
G Michailidis
Laboratory of Physiology of Reproduction of Farm Animals, Department of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, Greece
https://orcid.org/0000-0003-0624-7568
I Giannenas
Laboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki
Περίληψη

The testis is considered as an immune-privileged site, and testicular Sertoli cells have been identified as key players for conferring this immune privilege. These cells play an important physiological role in the testis, as they support, nourish and protect the germ cells, and present immune properties such as immune factors secretion, phagocytosis and constitute also the blood testicular barrier which limit damage to germ cells. The aim of the present study was to evaluate the inflammatory response in chicken Sertoli cells induced by metformin, an insulin-sensitizer drug which also possess anti-inflammatory properties, by studying the expression pattern of innate immune-related genes, including Toll-like receptors (TLRs), and cytokines, after a kinetic stimulation (0 to 48 hours) by metabolic dysregulation metformin induced. Chicken Sertoli cells were exposed to lipopolysaccharide (1µg/ml, as LPS used to mimic a bacterial infection) or metformin. Metformin stimulated expression of some Toll Like Receptors (TLR1-2; 2-1), but did not modify expression of the TLR4, known to be bound by LPS; as well as the increase in expression of some interleukins (IL-1β, IL-6, IL-8, IFN-y) after metformin stimulation and even more after an LPS and metformin exposure. In conclusion, modification of energy cell metabolism by metformin lead to activation of some set of immune system in the chicken Sertoli cells and even more after activation by bacterial component (LPS).

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Αναφορές
Akira S, Takeda K, Kaisho T (2001) Toll-like receptors: critical proteins linking innate and acquired immunity. Nat Immunol 2:675-680.
Anastasiadou M, Theodoridis A, Avdi M, Michailidis G (2011) Changes in the expression of Toll-like receptors in the chicken testis during sexual maturation and Salmonella infection. Anim Reprod Sci 128:93-99.
Anastasiadou M, Michailidis G (2016) Transcriptional changes of cytokines in rooster testis and epididymis during sexual maturation stages and Salmonella infection. Anim Reprod Sci 171:41-48.
Dufour JM, Rajotte RV, Korbutt GS, Emerich DF (2003) Harnessing the immunomodulatory properties of Sertoli cells to enable xenotransplantation in type I diabetes. Immunol Invest 32:275-297.
El-Zayat SE, Sibaii H, Mannaa FA (2019) Toll-like receptors activation, signaling, and targeting: an overview. Bull Natl Res Cent 43:187.
Fukumura D, Incio J, Shankaraiah R, Jain RK (2016) Obesity and cancer: an angiogenic and inflammatory link. Microcirculation 23:191-206.
Gerard N, Syed V, Jégou B (1992) Lipopolysaccharide, latex beads and residual bodies are potent activators of Sertoli cell interleukin-1a production. Biochem Biophys Res Commun 185:154-161.
Giri S, Nath N, Smith B, Viollet B, Singh AK, Singh I (2004) 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J Neurosci 24:479-87.
Gonzalez G (2015) Nutrient-Induced inflammation in polycystic ovary syndrome: Role in the Development of Metabolic Aberration and Ovarian Dysfunction. Semin Reprod Med 33:276-286.
Guma M, Wang Y, Viollet B, Liu-Bryan R (2015) AMPK activation by A-769662 controls IL-6 expression in inflammatory arthritis. PLoS One 10:1-13.
Herath S, Fischer DP, Werling D, Williams EJ, Lilly ST, Dobson H, Bryant CE, Sheldon IM (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus. Endocrinology 147:562-570.
Ibeagha-Awemu EM, Lee JW, Ibeagha AE, Bannerman DD, Paape MJ, Zhao X (2008) Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells. Vet Res 39:1-12.
Kaur G, Thompson LA, Dufour JM (2014) Sertoli cells-immunological sentinels of spermatogenesis. Semin Cell Dev Biol 30:36-44.
Kogut MH, Swaggerty C, He H, Pevzner I, Kaiser P (2006) Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses. Microbes Infect 8:1866-1874.
Labuzek K, Suchy D, Gabryel B, Bielecka A, Liber S, Okopien B (2010) Quantification of metformin by the HPLC method in brain regions, cerebrospinal fluid and plasma of rats treated with lipopolysaccharide. Pharmacol Rep 62:956-965.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-ΔΔC[T]) method. Methods 25:402-408.
Michailidis G, Theodoridis A, Avdi M (2010) Transcriptional profiling of Toll-like receptors in chicken embryos and in the ovary during sexual maturation and in response to Salmonella enteritidis infection. Anim Reprod Sci 122:294-302.
Morrison CD, Brannigan RE (2015) Metabolic syndrome and infertility in men. Best Pr Res Clin Obs Gynaecol 29:507-515.
O’Neill LJ, Hardie DG (2013) Metabolism of inflammation limited by AMPK and pseudo-starvation. Nature 493:346-355.
Park DW, Jiang S, Tadie JM, Stigler WS, Gao Y, Deshane J, Abraham E, Zmijewski JW (2013) Activation of AMPK enhances neutrophil chemotaxis and bacterial killing. Mol Med 19:387-398.
Pineau C, Le Magueresse B, Courtens JL, Jégou B (1991) Study in vitro of the phagocytic function of Sertoli cells in the rat. Cell Tissue Res 264:589-98.
Rena G, Pearson ER, Sakamoto K, (2013) Molecular mechanism of action of metformin: old or new insights? Diabetologia 56:1898-1906.
Riccioli A, Filippini A, De Cesaris P, Barbaccia E, Stefanini M, Starace G, Ziparo E (1995) Inflammatory mediators increase surface expression of integrin ligands, adhesion to lymphocytes, and secretion of interleukin 6 in mouse Sertoli cells. PNAS 92:5808-5812.
Selawry HP (1994) Islet transplantation to immunoprivileged sites. In Pancreatic Islet Transplantation: Immunomodulation of Pancreatic Islets 75-86.
Soraya H, Farajnia S, Khani S, Rameshard M, Khorrami A, Banani A, Maleki-Dizaji N, Garjani A (2012) Short-term treatment with metformin suppresses toll like receptors (TLRs) activity in isoproterenol-induced myocardial infarction in rat: are AMPK and TLRs connected? Int. Immunopharmacol. 14:785-791.
Starace D, Galli R, Paone A, De Cesaris P, Filippini A, Ziparo E, Riccioli A (2008) Toll-like receptor 3 activation induces antiviral immune responses in mouse Sertoli cells. Biol Reprod 79:766-775.
Stephan JP, Viqar Syed BJ (1997) Regulation of Sertoli cell IL-1 and IL-6 production in vitro. Mol Cell Endocrinol 134:109-118.
Sun B, Qi N, Shang T, Wu H, Deng T, Han D (2010) Sertoli cell-initiated testicular innate immune response through Toll-like receptor-3 activation is negatively regulated by Tyro3, Axl, and Mer receptors. Endocrinology 151:2886-2897.
Tomczynska M, Bijak M, Saluk J (2016) Metformin - the drug for the treatment of autoimmune diseases; a new use of a known anti-diabetic drug. Curr Top Med Chem 16:2223-2230.
Vaez H, Rameshrad M, Najafi M, Barar J, Barzegari A, Garjani A (2016) Cardioprotective effect of metformin in lipopolysaccharide-induced sepsis via suppression of toll-like receptor 4 (TLR4) in heart. Eur J Pharmacol 772:115-123.
Walzem RL, Chen S (2014) Obesity-induced dysfunctions in female reproduction: lessons from birds and mammals. Adv Nutr 5:199-206.
Weaver EA, Ramachandran R (2023) Metformin improves ovarian function and increases egg production in broiler breeder hens. Reproduction 165:289-300.
Wu H, Wang H, Xiong W, Chen S, Tang H, Han D (2008) Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology 149:4402-4412.
Xie F, Anderson CL, Timme KR, Kurz SG, Fernando SC, Wood JR (2016) Obesity-Dependent Increases in Oocyte mRNAs are associated with Increases in Pro-Inflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice. Endocrinology 157:1630-1643.
Yunhe F, Bo L, Xiaosheng F, Zhicheng L, Dejie L, Fengyang L, Depenga L, Yongguo C, Shuangc F, Xichen Z, Zhang N, Yang Z (2013) Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells. Veterinary Immunology and Immunopathology 151:20-27.
Zhang M, Nii T, Isobe N, Yoshimura Y (2012) Expression of Toll-like receptors and effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokine in the testis and epididymis of roosters. Poult Sci 90:2054-2062.
Zhang X, Howell GM, Guo L, Collage RD, Loughran PA, Zuckerbraun BS, Rosengart MR (2014) CaMKIV-Dependent Preservation of mTOR Expression Is Required for Autophagy during Lipopolysaccharide-Induced Inflammation and Acute Kidney Injury. J Immunol 193:2405-2415.
Zhao X, Zmijewski JW, Lorne E, Liu G, Park YJ, Tsuruta Y, Abraham E (2008) Activation of AMPK attenuates neutrophil proinflammatory activity and decreases the severity of acute lung injury. Am J Physiol Lung Cell Mol Physiol 295:497-504.
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