Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation

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Δημοσιευμένα: Jul 10, 2024
Ενημερώθηκε: 2024-07-10
Εκδόσεις:
2024-07-10 (2)
DOI: https://doi.org/10.12681/jhvms.34504
B Ustuner
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-5999-4685
MK Soylu
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-4690-1029
E Sengul
Department of Breeding Techniques, Sheep Breeding Research Institute, 10200 Bandırma, Balıkesir, Turkey
https://orcid.org/0000-0003-4918-0607
D Karakci
Department of Biochemistry, Faculty of Veterinary Medicine, Namık Kemal University, 59030, Tekirdag, Turkey
https://orcid.org/0000-0002-1884-1874
E Gokce
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Namık Kemal University, 59030, Tekirdag, Turkey
https://orcid.org/0000-0002-7678-3289
A Aktar
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-2975-2594
F Ak Sonat
Department of Physiology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-3308-0778
T Cimen
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-5280-9360
O Huraydin
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-4125-6841
MM Yılmaz
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-6050-791X
Z Nur
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-1438-221X
Περίληψη

This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen.


In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentrations of 1 mM (βG1), 2 mM (βG2), and 4 mM (βG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation, and determination of total antioxidant activity.


The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the βG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P<0.05). While the DNA fragmentation rate of the control group was higher than βG4 group (P<0.05), it was similar to βG1 and βG2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates.


In line with our results, supplementation of 1mM, 2 mM and 4 mM β-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.

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