Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation

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Publicado: Jul 10, 2024
Actualizado: 2024-07-10
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2024-07-10 (2)
DOI: https://doi.org/10.12681/jhvms.34504
B Ustuner
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-5999-4685
MK Soylu
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-4690-1029
E Sengul
Department of Breeding Techniques, Sheep Breeding Research Institute, 10200 Bandırma, Balıkesir, Turkey
https://orcid.org/0000-0003-4918-0607
D Karakci
Department of Biochemistry, Faculty of Veterinary Medicine, Namık Kemal University, 59030, Tekirdag, Turkey
https://orcid.org/0000-0002-1884-1874
E Gokce
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Namık Kemal University, 59030, Tekirdag, Turkey
https://orcid.org/0000-0002-7678-3289
A Aktar
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-2975-2594
F Ak Sonat
Department of Physiology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-3308-0778
T Cimen
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-5280-9360
O Huraydin
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-4125-6841
MM Yılmaz
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0001-6050-791X
Z Nur
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Görükle, Bursa, Turkey
https://orcid.org/0000-0002-1438-221X
Resumen

This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen.


In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentrations of 1 mM (βG1), 2 mM (βG2), and 4 mM (βG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation, and determination of total antioxidant activity.


The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the βG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P<0.05). While the DNA fragmentation rate of the control group was higher than βG4 group (P<0.05), it was similar to βG1 and βG2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates.


In line with our results, supplementation of 1mM, 2 mM and 4 mM β-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.

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Citas
Aisen EG, Alvarez HL, Venturino A, Garde JJ (2000) Effect of trehalose and
EDTA on cryoprotective action of ram semen diluents. Theriogenology
(5):1053-1061. https://doi.org/10.1016/S0093-691X(00)00251-X.
Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, Irvine
DS (1998) Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod
(5):1037-1046. https://doi.org/10.1095/biolreprod59.5.1037.
Aitken RJ, Baker MA (2004) Oxidative stress and male reproductive biology, Reprod Fertil Dev 16:581-588. https://doi.org/10.10371/
RD03089 .
Aitken RJ, Jones KT, Robertson SA (2012) Reactive oxygen species and
sperm function in sickness and in health. J Androl 33(6):1096-1106.
https://doi.org/10.2164/jandrol.112.016535.
Ak Sonat F, Alcay S, Toker MB, Peker S, Ustuner B (2018) The effects of
dietary restriction and administration of β-glucan from Euglena gracilis
on the sperm characteristics and reproductive organs of rats. Andrologia 50(9):e13088. https://doi.org/10.1111/and.13088.
Alvarez JG, Storey BT (1989) Role of glutathione peroxidase in protecting
mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 23:77-90. https://doi.org/10.1002/
mrd.1120230108 .
Amaral A, Lourenço B, Marques M, Ramalho-Santos J (2013) Mitochondria functionality and sperm quality. Reproduction (Cambridge, England) 146(5):163-174. https://doi.org/10.1530/REP-13-0178.
Atessahin A, Bucak MN, Tuncer PB, Kızıl M (2008) Effects of anti-oxidant
additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rum Res 77(1):38-
https://doi.org/10.1016/j.smallrumres.2008.03.002.
Azimi G, Farshad A, Farzinpour A, Rostamzadeh J, Sharafi M (2020) Evaluation of used Purslane extracts in Tris extenders on cryopreserved
goat sperm. Cryobiology 94:40-48. https://doi.org/10.1016/j.cryobiol.2020.05.001 .
Barsanti L, Passarelli V, Evangelista V, Frassanito AM, Gualtieri P (2011)
Chemistry, physico-chemistry and applications linked to biological activities of β-glucans. Nat Prod Rep 28(3):457-466. https://doi.
org/10.1039/c0np00018c.
Bashir KMI, Choi JS (2017) Clinical and physiological perspectives of
β-glucans: the past, present, and future. Int J Mol Sci 18(9):1906. https://doi.org/10.3390/ijms18091906.
Bell M, Wang R, Hellstrom WJ, Sikka SC (1993) Effect of cryoprotective
additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm. J Androl 14:472-478.
https://doi.org/10.3390/ijms18091906.
Breivik T, Opstad PK, Engstad R, Gundersen G, Gjermo P, Preus H (2005)
Soluble β‐1, 3/1, 6‐glucan from yeast inhibits experimental periodontal disease in Wistar rats. Int J Mol Sci 32(4):347-352. https://doi.
org/10.3390/ijms18091906.
Bucak MN, Tuncer PB, Sariozkan S, Ulutas PA, Coyan K, Baspinar N, Ozkalp B (2009) Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen. Res Vet Sci 87(3):468-472. https://doi.org/10.1016/j.
rvsc.2009.04.014.
Bucak MN, Gungor Ş, Sariozkan S, Bilgili A (2015) Spermanın dondurulmasında antioksidanların etkisi. Türkiye Klinikleri 1(3):39-47.
Collodel G, Moretti E, Noto D, Corsaro R, Signorini C (2022) Oxidation of
polyunsaturated fatty acids as a promising area of research in infertility. Antioxidants 11(5):1002. https://doi.org/10.3390/antiox11051002.
Cotter P, Goolsby H, Prien S (2005) Preliminary evaluation of a unique
freezing technology for bovine spermatozoa cryopreservation.
Reprod Domest Anim 40:98-99. https://doi.org/10.1111/j.1439-
2004.00540.x.
Cui W, Wood PJ (2000) Relationships between structural features, molecular weight and rheological properties of cereal β-D-glucans. In: Hydrocolloids, Elsevier, Amsterdam: pp 159-168. https://doi.org/10.1016/
B978-044450178-3/50019-6.
Del Olmo E, Bisbal A, Maroto-Morales A, García-Alvarez O, Ramon M,
Jimenez-Rabadan P, Martínez-Pastor F, Soler AJ, Garde JJ, Fernandez-Santos MR (2013) Fertility of cryopreserved ovine semen is determined by sperm velocity. Anim Reprod Sci 138 (1-2): 102-109. https://
doi.org/10.1016/j.anireprosci.2013.02.007.
Dietrich-Muszalska A, Olas B, Kontek B, Rabe-Jabłońska J (2011) Beta-glucan from Saccharomyces cerevisiae reduces plasma lipid peroxidation induced byhaloperidol. Int J Biol Macromol 49(1): 113-116.
https://doi.org/10.1016/j.ijbiomac.2011.03.007.
Divya M, Karthikeyan S, Ravi C, Govindarajan M, Alharbi NS, Kadaikunnan S, Khaled JM, Almanaa TN, Vaseeharan B (2020) Isolation of β-glucan from Eleusine coracana and its antibiofilm, antidiabetic, antioxidant, and biocompatible activities. Microb Pathog 103955. https://doi.org/10.1016/j.micpath.2019.103955.
Doublier JL, Wood PJ (1995) Rheological properties of aqueous solutions
of (1--> 3)(1--> 4)-bD-glucan from oats (Avena sativa L.). Cereal
Chemi 72 (4): 335-340.
Gulmen S, Kiris I, Kocyigit A, Dogus DK, Ceylan BG, Meteoglu I (2010)
β-Glucan protects against lung injury induced by abdominal aortic
ischemia-reperfusion in rats. J Surg Res 164(2):325-332. https://doi.
org/10.1016/j.jss.2010.08.013.
Harada T, Ohno N (2008) Contribution of dectin-1 and granulocyte macrophage-colony stimulating factor (GM-CSF) to immunomodulating
actions of β-glucan. Int Immunopharmacol 8(4): 556-566. https://doi.
org/10.1016/j.intimp.2007.12.011.
Holt WV, Penfold LM (2014) Fundamental and practical aspects of semen
cryopreservation. In: Animal Andrology: Theories and Applications.
CAB International, UK: pp 76-100. https://doi.org/10.1079/97817806
0076.
Inanc ME, Gungor S, Ozturk C, Korkmaz F, Bastan I, Cil B (2019)
Cholesterol‐loaded cyclodextrin plus trehalose improves quality of frozen‐thawed ram sperm. Vet Med 64:118-124. .https://doi.
org/10.17221/146/2018-VETMED.
Ismail AA, Abdel-Khalek AE, Khalil WA, Yousif AI, Saadeldin IM,
Abomughaid MM, El-Harairy MA (2020) Effects of mint, thyme, and
curcumin extract nanoformulations on the sperm quality, apoptosis,
chromatin decondensation, enzyme activity, and oxidative status of
cryopreserved goat semen. Cryobiology 97:144-152. https://10.1016/j.
cryobiol.2020.09.002.
Kaya K, Ciftci O, Aydın M, Cetin A, Basak N (2019) Favourable effect of
β‐glucan treatment against cisplatin‐induced reproductive system damage in male rats. Andrologia 51(9): e13342. https://doi.org/10.1111/
and.13342.
Kayali H, Ozdag MF, Kahraman S, Aydin A, Gonul E, Sayal A, Odabasi Z,
Timurkaynak E (2005) The antioxidant effect of β-Glucan on oxidative
stress status in experimental spinal cord injury in rats. Neurosurg Rev
:298-302. https://doi.org/10.1007/s10143-005-0389-2.
Lei N, Wang M, Zhang L, Xiao S, Fei C, Wang X, Zhang K, Zheng W,
Wang C, Yang R, Xue F (2015) Effects of low molecular weight yeast
β-glucan on antioxidant and ımmunological activities in mice. Int J Mol
Sci 16(9):21575-21590. https://doi.org/10.3390/ijms160921575.
Liang J, Larbi A, Lv C, Ali S, Wu G, Quan G (2023) Fertility results after
exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin. Reprod Domest
Anim 58:431-442. https://doi.org/10.1111/rda.14304.
Longobardi V, Zullo G, Cotticelli A, Salzano A, Albero G, Navas L, Rufrano D, Claps S, Neglia G (2020) Crocin improves the quality of cryopreserved goat semen in different breeds. Animals 26: 10(6):1101. https://
doi.org/10.3390/ani10061101.
Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M, Nadia FM, Audrey G (2011) Effect of butylated hydroxytoluene on cryopreservation
of Boer goat semen in Tris egg yolk extender. Anim Repro Sci 129(1-
:44-49. https://doi.org/10.1016/j.anireprosci.2011.10.004.
Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M, Nadia FM
(2013) Effect of ascorbic acid concentrations, methods of cooling and
freezing on Boer goat semen cryopreservation. Reprod Domest Anim
(2):325-330. https://doi.org/10.1111/j.1439-0531.2012.02155.x.
Merati Z, Farshad A (2021) Supplementary role of vitamin E and amino acids added to diluent on goat sperm freezability. Cryobiology 100:151-
https://doi.org/10.1016/j.cryobiol.2021.02.004.
Morris ID, Ilott S, Dixon L, Brison DR (2002) The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet
assay) and its relationship to fertilization and embryo development.
Hum Reprod 17(4):990-998. https://doi.org/10.1093/humrep/17.4.990.
Mortimer ST (2000) CASA - practical aspects. J Androl 21:515-524. https://
doi.org/10.1002/j.1939-4640.2000.tb02116.x.
Mustofa I, Susilowati S, Suprayogi TW, Oktanella Y, Purwanto DA, Akintunde AO (2023) Combination of nanoparticle green tea extract in
tris-egg yolk extender and 39 °C thawing temperatures improve the
sperm quality of post-thawed Kacang goat semen. Anim Reprod 19(4):
e20220025. https://doi.org/10.1590/1984-3143-AR2022-0025.
Noiles EE, Bailey JL, Storey BT (1995) The temperature dependence in
the hydraulic conductivity, Lp, of the mouse sperm plasma membrane
shows a discontinuity between 4 and 0 °C. Cryobiology 32(3):220-238.
https://doi.org/10.1006/cryo.1995.1022.
Nowicka-Bauer K, Nixon B (2020) Molecular changes induced by oxidative stress that impair human sperm motility. Antioxidants 9(2):134.
https://doi.org/10.3390/antiox9020134.
Nur Z, Zik B, Ustuner B, Sagirkaya H, Ozguden CG (2010) Effects of
different cryoprotective agents on ram sperm morphology and DNA
integrity. Theriogenology 73(9):1267-1275. https://doi.org/10.1016/j.
theriogenology.2009.12.007.
Quinn PJ, Chow PYW, White IG (1980) Evidence that phospholipid protects ram spermatozoa from cold shock at a plasma membrane site.
J Reprod Fertil 60(2):403-407. https://doi.org/10.1530/jrf.0.0600403.
Ren F, Feng T, Dai G, Wang Y, Zhu H, Hu J (2018) Lycopene and alpha-lipoic acid improve semen antioxidant enzymes activity and cashmere
goat sperm function after cryopreservation. Cryobiology 84:27-32.
https://doi.org/10.1016/j.cryobiol.2018.08.006.
Rezaei A, Bahmani HR, Mafakheri S, Farshad A, Nazari P, Masoudi R
(2023) Protective effects of different doses of MitoQ separately and
combined with trehalose on oxidative stress and sperm function of
cryopreserved Markhoz goat semen. Cryobiology 110:36-43. https://
doi.org/10.1016/j.cryobiol.2022.12.019.
Richter J, Svozil V, Král V, Rajnohová Dobiášová L, Vetvicka V (2015)
β-glucan affects mucosal immunity in children with chronic respiratory
problems under physical stress: clinical trials. Ann Transl Med 3(4):52.
https://doi.org/10.3978/j.issn.2305-5839.2015.03.20.
Ruiz-Pesini E, Díez-Sánchez C, López-Pérez MJ, Enríquez JA (2007) The
role of the mitochondrion in sperm function: is there a place for oxidative phosphorylation or is this a purely glycolytic process? Curr Top
Dev Biol 77:3-19. https://doi.org/10.1016/S0070-2153(06)77001-6.
Salmani H, Nabi MM, Vaseghi-Dodaran H, Rahman MB, Mohammadi-Sangcheshmeh A, Shakeri M, Zhandi M (2013) Effect of glutathione in soybean lecithin-based semen extender on goat semen quality
after freeze-thawing. Small Rumin Res 112(1-3):123-127. https://doi.
org/10.1016/j.smallrumres.2012.12.015.
Selli J, Unal D, Mercantepe F, Akaras N, Kabayel R, Unal B, Atilay H
(2016) Protective effects of beta glucan in brain tissues of post-menopausal rats: a histochemical and ultra-structural study. Gynecol Endocrinol 32(3):234-239. https://doi.org/10.3109/09513590.2015.1110139
Sener G, Toklu H, Ercan F, G Erkanli (2005) Protective effect of β-glucan
against oxidative organ injury in a rat model of sepsis. Int immunopharmacol 5(9) :1387-1396. https://doi.org/10.1016/j.intimp.2005.03.007.
Sener G, Eksioglu-Demiralp E, Cetiner M, Ercan F, Yegen BC (2006)
β-glucan ameliorates methotrexate-induced oxidative organ injury via
its antioxidant and immunomodulatory effects. Eur J Pharmacol 542(1-
:170-178. https://doi.org/10.1016/j.ejphar.2006.02.056.
Skendi A, Biliaderis CG, Lazaridou A, Izydorczyk MS (2003) Structure and
rheological properties of water soluble β-glucans from oat cultivars of
Avena sativa and Avena bysantina. J Cereal Sci 38(1):15-31. https://
doi.org/10.1016/S0733-5210(02)00137-6.
Slamenová D, Lábaj J, Krizková L, Kogan G, Sandula J, Bresgen N, Eckl
P (2003) Protective effects of fungal (1-->3)-beta-D-glucan derivatives
against oxidative DNA lesions in V79 hamster lung cells. Cancer Lett
(2):153-160. https://doi.org/10.1016/s0304-3835(03)00336-7.
Sun L, Fan W, Wu C, Zhang S, Dai J, Zhang D (2020) Effect of substituting
different concentrations of soybean lecithin and egg yolk in tris-based
extender on goat semen cryopreservation. Cryobiology 92:146-150.
https://doi.org/10.1016/j.cryobiol.2019.12.004.
Talukdar DJ, Ahmed K, Sinha S, Deori S, Das GC, Talukdar P (2017) Cryopreservation induces capacitation-like changes of the swamp buffalo
spermatozoa. Buffalo Bull 36(1)-221-230.
Ustuner B, Nur Z, Alcay S, Toker MB, Sagirkaya H, Soylu MK (2015)
Effect of freezing rate on goat sperm morphology and DNA integrity.
Turk J Vet Anim Sci 39 (1):110-114. https://doi.org/10.3906/vet-1407-
Ustuner B, Gokce E, Toker MB, Onder NT, Soylu MK, Sagirkaya H, Nur Z
(2018) Effect of sperm pooling with seminal plasma collected i
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