Oxidative stress markers in canine parvovirus infection


Δημοσιευμένα: Oct 22, 2024
A Khoshvaghti
https://orcid.org/0000-0002-8577-0439
Ν Baziyari
M Dehghani
F Vaziri
Περίληψη

Parvovirus infections are among the deadly viral diseases in dogs and sick puppies. Young dogs are more prone to the virus. Antioxidants are important components of the defense system which prevent cell damage by neutralizing reactive oxygen species. Malondialdehyde is a reliable and commonly used marker of oxidative stress. The present study was performed to determine the role of oxidative stress markers in canine parvovirus disease.


Thirty Cane Corso male dogs with an average age of 3-6 months were enrolled in this study out of which 10 animals were spontaneously infected with the parvovirus and the other 10 were healthy. The activity of superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde were measured by colorimetric methods whereas the enzymatic activity of glutathione peroxidase (GPX) was measured. Catalase was measured by means of rapid spectrophotometry and chromography was used for obtaining the concentrations of vitamin D and vitamin E.


The increase in malondialdehyde in infected dogs demonstrates that oxidative stress plays a role in the pathogenesis of the parvovirus. The significant elevation in TAC, and lack of important changes in superoxide dismutase and catalase are indicative of a good response by the antioxidant system against oxidative stress induced by the parvovirus. In conclusion, oxidative stress is involved in the pathogenesis of parvovirus. Thus, strengthening the antioxidant system can be effective in the prevention and treatment of canine parvovirus infection.

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Αναφορές
Mazzaferro EM: Update on Canine Parvoviral Enteritis . Veterinary
Clinics of North America: Small Anim Pract 2020;50(6):1307-1325.
Mathios E ,Mylonakis Kalli I, Timoleon SR :Canine parvoviral enteritis: an update on the clinical diagnosis, treatment, and prevention.
Veterinary Medicine: Res Rep Trop Med Dove Press2016; 7: 91-100.
Decaro N, Martella V, Desario C, Bellacicco A.L, Camero M, Manna
L, d’Aloja D, Buonavoglia C: First detection of canine Parvovirus
type 2c in pups with haemorrhagic enteritis in Spain. J Vet Med B
infect Dis Vet Public Health 2006; 53(10):468-472.
Schaudien D, Polizopoulou Z, Koutinas A, Schwab S, Porombka
D, Baumgärtner W, Herden C: Leukoencephalopathy associated
with Parvovirus infection in Cretan hound puppies. J Clin Microb
;48(9):3169-3175.
Tóthová L, Celecová V, Celec P. Salivary markers of oxidative stress
and their relation to periodontal and dental status in children. Dis
Markers 2013; 34(1): 9-15.
Abdolsamadi H, Rafieian N, Goodarzi MT, Feradmal J, Davoodi P,
Jazayeri M, Taghavi Z, Hoseyni SM, Ahmadi-Motamayel F . Levels of salivary antioxidant vitamins and lipid peroxidation in patients
with oral lichen planus and healthy individuals. Chonnam Med J
; 50(2): 58-62.
Crnogaj M, Cerón JJ, Šmit I, Kiš I, Gotić J, Brkljačić M, Matijatko V,
Peres Rubio C, Kučer N, Mrljak V: Relation of antioxidant status at
admission and disease severity and outcome in dogs naturally infected with Babesia canis. BMC Vet Res 2017;13:114.
Ukwueze S, Akpan ES, Ezeokonkwo RC, Nwosuh CI, Anene BM:
Haematological, oxidative stress and electrolyte alterations in puppies with canine parvoviral enteritis. Iraqi J Vet Sci 2020; 34( 1):65-
Dodds WJ: Biomarkers of oxidative stress in dogs. Med Res Arch
;8 ( 5).
Gaweł S , Wardas M, Niedworok E, Wardas P: Malondialdehyde
(MDA) as a lipid peroxidation marker. Wiadomości Lekarskie 2004;
(9-10):453-5.
Kayar B, Dokuzeylul FM, Kandemir A, Kirbas A and Bayrakal ME.
Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde concentrations in cats seropositive for the feline coronavirus.
Vet Med 2015;6 (5): 274-281.
Gaykwad C J, Garkhal GE, Chethan SN, De UK: Stress using N‐acetylcysteine in canine parvoviral enteritis. J Vet Pharma Therapy 2018;
(1): 68-75.
Rubio CP, Martínez-Subiela S, Hernández-Ruiz J, Tvarijonaviciute A , Cerón J J , Allenspach K: Serum biomarkers of oxidative
stress in dogs with idiopathic inflammatory bowel disease . Vet J
, 221:56-61.
Panda D, Patra RC , Nandi S, Swarup D.Oxidative stress indices in
gastroenteritis in dogs with canine parvoviral infection. Res Vet Sci
Res 2009;86(1):36-42.
Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A
novel method for measuring antioxidant capacity and its application
to monitoring the antioxidant status in premature neonates. Clinic Sci
; 84:407-12.
SIES H. Strategies of antioxidant defense. European J Biochem
;215: 213-219.
Streck AF, Rüster D, Truyen U, Homeier T. An updated TaqMan
real-time PCR for canine and feline Parvoviruses. J Virol Methods
; 193(1): 6-8.
Arthur JR, Boyne R. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper
deficient cattle. Life Sci 1985;22,36(16),1569-75.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative
characterization of erythrocyte glutathione peroxidise. J Lab Clinic
Med 1967;70:158-169.
Esterbauer H, Cheeseman KH. Determination of aldehydic lipid
peroxidation products: malonaldehyde and 4-hydroxynonenal. Meth
Enzym 1990;186:407-21.
Góth L. A simple method for determination of serum catalase activity
and revision of reference range. Clinica Chimica Acta 1991;196(2-
:143-51.
Norusis MY. Spss for windows base system user’s guide release 6.0,
st ed. Spss Inc, Michigon.1993; 281-290.
Halliwell B .The definition and measurement of antioxidants in biological system. Free Radical Biology Med 1995;18,125-126.
Chapple ILC. Reactive oxygen species and antioxidants in inflammatory diseases. J Clinic Periodontal 1997;24:287-296. 22.
Noha M. Elsayed NM, Kubesy AA,Salem NY. Altered blood oxidative stress biomarkers in association with canine Parvovirus enteritis.
Com Clin Path 2020;29:355-359.
Mathios E Mylonakis ME, Kalli I, Rallis TS. Canine parvoviral enteritis: an update on the clinical diagnosis, treatment, and prevention.
Vet Med (Auckl).2016; 7: 91-100.
Harizan IM, Khinchi RK, Sharma MSK, Meena VK: Alterations in
haemato-biochemical and oxidative stress indices in dogs affected
with parvoviral enteritis. NAAS Rating 2021;10( 8s):428-431. DOI:
Panda D, Patra RC, Nandi S, Swarup D:Oxidative stress indices in
gastroenteritis in dogs with canine parvoviral infection. Res Vet Sci
;86:36-42.
Kataria D, Agnihotri D, Kumar S, Archana L: Comparative assessment of oxidative stress in dogs regarding CPV infection. Pharmaceutical innovation J 2020; 9(2): 115-117.
Janciauskiene S.The Beneficial Effects of Antioxidants in Health and
Diseases .Chronic Obstr Pulm Dis 2020; 7(3): 182-202.
Bohn T: Carotenoids and markers of oxidative stress in human obsorvational studies and intervention trails: Implicatioms for chronics
diseases . Antioxidants 2019;8 (6) ;179.
Jewell D, Yu s , Joshi D.Effects of serum vitamin E concentrations
on skin vitamin E concentrations in dogs and cats. Vet Therapy
;3(3):235-243
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