A stereological study: Volume estimation in the cerebellum of Norduz sheep
Abstract
In this research, it was aimed to investigate the cerebellum volume values of Norduz sheep raised within the borders of Gurpinar district of Van province. For this purpose, 6 male and 6 female Norduz sheep heads were selected as materials. The brains were dissected and kept in formaldehyde for a week. Cerebellums were separated from the brain hemispheriums. For better fixation, the cerebellums were kept in formaldehyde again for a week. Tissues obtained by sequential random sampling were included in tissue tracking. Then each tissue was embedded in paraffin. An average of 10-12 sections with a thickness of 5µm were obtained from the tissue cassettes obtained by a Rotary microtome (Leica RM, 2135, Nussloch Germany). Sections stained with hematoxylin and eosin were photographed with an x4 objective. From these photographs, total cerebellum, medulla and cortex volumes, as well as vermis volume, were calculated using the Shtereom I program using the Cavalieri Principle. The results were evaluated statistically with the Mann-Whitney U test. No statistical difference was detected in terms of male and female cerebellum volume values, Coefficient of Error (CE) and Noise calculations (p>0.05). However, in the evaluation between genders, it was determined that the only difference was seen in the medulla section of the hemispherium cerebelli sinister (p<0.05).
Keywords: Cavalieri Principle; cerebellum; Norduz sheep; stereology; volume.
Article Details
- How to Cite
-
Kiran, M., & Cakmak, G. (2024). A stereological study: Volume estimation in the cerebellum of Norduz sheep. Journal of the Hellenic Veterinary Medical Society, 75(3), 8041–8054. https://doi.org/10.12681/jhvms.36423
- Issue
- Vol. 75 No. 3 (2024)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
