Evaluation of the use of L-carnosine in short-term storage of ram semen with spermatological and flow-cytometric analyzes
Resumen
This study was conducted to investigate the effect of adding different doses of L-carnosine to semen in vitro on the short-term storage of semen. Semen taken from 6 Akkaraman rams was used in the research. Semen taken from each animal was pooled. It was diluted with tris+egg yolk extender containing L-carnosine at different doses (1, 5, 10, 20, 50, 100 and 200 mM) and stored at +4°C for 96 hours. It was determined that 1, 5, 10, 20 mM L-carnosine doses increased total, progressive and rapid motility, kinematic parameters VCL, VSL, VAP values and high mitochondrial membrane potential level during short-term storage. It was also determined that these doses protected membrane integrity, reduced the rate of abnormal spermatozoon, acrosomal damage and low mitochondrial membrane potential level. It was determined that a dose of 200 mM L-carnosine had a toxic effect and negatively affected the parameters.
As a result, when ram semen is desired to be stored for a short time (for 96 hours), it may be recommended to add 1, 5, 10 and 20 mM L-carnosine amino acid to the semen extenders in order to preserve spermatological parameters.
Article Details
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Güngör, İH, Gür, S., Koca, R., Çakır Cihangiroğlu, A., Güler Ekmen, E., Acısu, T., Badıllı, N., Özer Kaya, Ş, Yüce, A., Sönmez, M., & Türk, G. (2024). Evaluation of the use of L-carnosine in short-term storage of ram semen with spermatological and flow-cytometric analyzes. Journal of the Hellenic Veterinary Medical Society, 75(3), 7977–7992. https://doi.org/10.12681/jhvms.36212
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- Vol. 75 Núm. 3 (2024)
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- Research Articles
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